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Tannins
Tannins
Safdar munir
Introduction
The term tannin was first time coined by Seguin in 1796. This
term was used to denote substances present in plant extract
which react with protein of animal hide, prevent their
.putrefaction and convert hide and skin into leather
Definition
Complex substances that usually occur as mixtures of“
polyphenols that are very difficult to separate since they don't
.”crystallize, are called tannins
OR
i) Precipitation)
ii) Anti-oxidizing properties)
iii) Astringent)
iv) Carcinogenicity)
v) Reaction with salts)
vi) Reaction with potassium ferricyanide and ammonia)
i) Precipitation)
;Tannins have ability to precipitate solutions of
Gelatin
Alkaloids
Glycosides
Heavy metals
Proteins
iv) Carcinogenicity)
Prolong use of tannin containing plant material is hazardous
because it causes cancer. Habitual use of Areca catechu can
.cause oral and esophageal cancer
v) Reaction with salts)
Tannins
:Precursors
(Phenolic acid (Gallic acid, Ellagic acid
Glucose residue
Between phenolic acids and glucose sugar, there is
ester linkage
:Properties
:Types
Hydrolysable tannins
Gallitannins Ellagitannins
Gallitannins Ellagitannins
:Precursors
Flavonoid
Catechin
Flavonol-3-4-diol
:Properties
When heated with acids, these are self condensated,
polymerized and converted to insoluble red colored
.complexes, called Phlobaphenes
:Examples
Hamamelis
Cinchona
Cinnamon
iii) Complex tannins)
These tannins are mixtures of both, hydrolysable and
condensed tannins
:Examples
Tea
Quercus
Castanea
2nd Classification
Tannin is a substance which is detected qualitatively by tanning
test (The Gold beater’s skin test) and quantitatively by its
.adsorption on standard hide powder
Tannins
:Gelatin test
Solution of tannin (0.5%-1%) precipitates 1% solution of gelatin
.containing 10% sodium chloride
Identification tests
:Phenazone test
.Take 5ml of aqueous extract of drug
.Add 0.5grams of sodium acid phosphate
.Warm it and cool
.Filter solution
.To the filtrate, add 2%solution of Phenazone
.Tannins will be precipitated
.Precipitates will be bulky and colored
Identification tests
:Gold beater’s skin test
Gold beater’s skin is a membrane prepared from intestine of Ox
.and I behaves similarly to un tanned skin
Natural tannins are used to Get leather from animal skin in: 1
(.tanneries.(now a days synthetic tannins are used
When incubated with red grape juice and red wines with a; <
high quantity of condensed tannins the Polio virus,Herpes
.simplex virus and various enteric viruses are inactivated
:Family
Hamamelidaceae
:Part used
Dried leaves
:Collection
Leaves are collected throughout
summers. Drying is done in
open air, preferably under
shade to preserve green
.color
:Constituents
Gallitannins
Ellagitannins
Free gallic acid
Pro-anthocyanadins
Hamamelase
:Uses
Astringent
Anti-hemorrhagic
Anti-inflammatory
Anti-carcinogenic
b) Catechu
:Botanical origin
Uncaria gambir
:Family
Rubiaceae
:Part used
Dried aqueous extract from leaves and
young twigs
:Collection
Boil leaves and twigs in water.
Evaporate to a pasty consistency.
Paste is put in cubes and dried in
.sun
:Constituents
Catechin
Catechutannic acid
Catechu red
Quercitin
Gambir flourescin
:Uses
Astringent
Dying
Tanning industry
c) Nutt gall
:Botanical origin
Quercus infectoria
:Family
Fagaceae
:Part used
Galls obtained from
twigs
:Formation of galls
Galls are pathological outgrowth formed on twigs of tree. Galls
arise due to deposition of eggs by small insect Adleria
.galactinctoria
;Steps are
Early summers, insects lay eggs on twigs
.Larvae come out from eggs and enter soft epidermis
Larvae secretes enzyme that stimulates abnormal growth around
.larvae
Starch in the tissue gets converted to sugars and stimulate cell
.division
.Disappearance of starch causes cell shrinkage
. Central cavity is formed in which larvae and pupae grows
.Larvae remains in gall for 5-6months
.Mature insect bores the covering of gall and escapes away
.Galls are collected before escaping of insect
:Constituents
Tannic acid
Gallic acid
Ellagic acid
Roburic acid
Syringic acid
Calcium oxalate crystals
:Uses
Haemostatic
Antidote for alkaloidal poisoning
Astringent
The end
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