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B.SC Microbiology Syllabus
B.SC Microbiology Syllabus
Sc MICROBIOLOGY - SYLLABUS
SEMESTER - I
UNIT I
of bacteria and fungi. Culture media and pure culture techniques. Anaerobic
culture techniques.
UNIT II
properties.
UNIT III
nutrients by active and passive transport, concepts of free energy. Energy rich
UNIT IV
Glycolysis, TCA cycle- peculiar pathways- HMP, ED, PPP, glyoxylate
pathway, glycolic acid cycle. Fermentation – lactic acid, propionic acid, acetic
UNIT V
UNIT I
Cleaning of glass wares Sterilization principle and methods- moist heat- dry heat
and filtration methods. Media preparation: liquid media, solid media, agar slants,
agar plates, basal, enriched, selective media preparation- quality control of media,
UNIT-II
Pure culture techniques: streak plate, pour plate, decimal dilution. Culture
UNIT-III
Microscopy: light microscopy, bright field microscopy, dark field
field microscopy, semi solid agar, Craigie’s tube method. Staining techniques:
smear preparation, simple staining, Gram’s staining, acid fast staining, staining of
Metachromatic granules.
UNIT-IV
UNIT-V
UNIT I
UNIT-II
UNIT III
Structure and bonding: Ionic and covalent bonding M.O. and V.B.
,bond angle and bond energy, hydrogen bonding, van der Waals interactions. Ionic
UNIT IV
S.P and d Block Elements: Oxides, halides and hydrides of alkali and alkaline
earth metals, B, Al, S,N,P and S, silicones, general characteristics of 3d elements.
UNIT-V
Coordination complexes: valence bond and crystal field theory, color, geometry
SEMESTER II
UNIT I
Vaccine,
UNIT II
UNIT III
UNIT IV
resistance.
UNIT V
mapping Lac operon – trp operon. Mutations – various types & Mechanism; Ames
UNIT-I
Agglutination reactions: Slide and Tube methods RBC agglutination IHA, TPHA
Bacterial.
UNIT-II
UNIT-III
Enumeration of different cell types, Perpheral blood cell counts, absolute cell
counts.
UNIT-IV
(Demonstration).
UNIT-V
UNIT I
solution, solubility product, common ion effect, hydrolysis of salts, pH, buffer and
UNIT II
UNIT III
energy, zero, first and second order kinetics, equilibrium constants (Kc, KP and
UNIT IV
Thermodynamics: First law, reversible and irreversible processes, internal
formation, Second law, entropy, free energy, and work function. Gibbs-Helmholtz
UNIT V
REFERENCE BOOKS
1. Prescott, L.M., Harley, J.P. and Klein, D.A. (1999) Microbiology. McGraw
Hill, New Delhi
2. Ketchum, P.A. (1984) Microbiology: Concepts and Applications. John Wiley
and Sons, New York.
3. Madigan, M.T., Martinko, J.M. and Parker, J. (1999). Brock’s Biology of
Micro organisms, 9th Edn. Prentice Hall, New Jersey.
4. Salle, A.J. (1992) Fundamental Principles of Bacteriology 7th Edn. Tata
McGraw Hill, New Delhi.
5. Mandelstam, J., McQuillen, K. and Dawes, L. (1992) Biochemistry of
Bacterial Growth, 3rd Edn. Blackwell Scientific Publications, Oxford.
6. Doelle, H.W. (1975) Bacterial Metablism. 2nd Edn. Academic Press, London.
7. Moat, A.G. and Foster, J.W. (1995) Microbial Physiology, 3rd Edn. John
Wiley and Sons, New York.
8. Roitt, I.M.(1988) Essential Immunology. Blackwell Scientific
Publications,Oxford.
9. Jewetz, E., Melnic, J.L. and Adelberg, E.A. (2000) Review of Medical
Microbiology ,19th Edn. Lange Medical Publications, U.S.A.
10. Ananthanarayan, R. and Jeyaram Paniker, C.K. (1994) Text Book of
Microbiology,6th Edn. Orient Longman, Chennai.
11. Jeyaram Paniker, C.K. (2006) Text Book of Parasitology. Jay Pee Brothers,
New Delhi.
12. Alexender, M. (1977) Introduction to Soil Microbilogy. John Wiley and
Sons, New York.
13. Stanbury, P.F., Whitaker, A. and Hall, S.J. (1995) Principles of
Fermentation Technology, 2nd Edn. Pergamon Press, Oxford.
14. Frazier, W.C. and Westhoff, D.C. (1988) Food Microbiolog,. 4th Edn.
McGraw Hill, New York.
15. Old, R. and Primrose, S.B. (1995) Principles of Gene Manipulation: An
Introduction to Genetic Engineering, 5th Edn. Blackwell Scientific
Publications, Oxford.
16. Freifelder, D. (1995) Molecular Biology. Narosa Publishing House, New
Delhi.
17. Timbury, M.C. (1986) Medical Virology, 9th Edn. Churchill Livingstone,
London.
18. Finegold, S.M. (2000) Diagnostic Microbiology, 10th Edn. C.V. Mosby
Company, St. Louis
19. Jagadish Chander (1996) A Text Book of Medical Mycology. Interprint, New
Delhi.
20. Greenwood, D., Slack, R.B. and Peutherer, J.F.(2002) Medical Microbiology,
16th Edn. Churchill Livingstone, London.
21. Arora, D.R. (2003) Text Book of Microbiology, 2nd Edn. CBS Publishers &
Distributors, New Delhi.
22. Arora, D.R. and Arora, B.(2002) Medical Parasitology, 1st Edn. CBS
Publishers & Distributors, New Delhi.
23. Dubey, R.C. and Maheswari, D.K. (2003) A Text Book of Microbiology, 1st
Edn. S. Chand & Co. Ltd., New Delhi.
24. Dubey, R.C. and Maheswari, D.K. (2002) Practical Microbiology, 1st Edn.
S. Chand & Co. Ltd., New Delhi
25. Sathyanarayana, U. (2002) Essentials of Biochemistry 1st Edn. Books and
Allied (P) Ltd., Kolkata.
26. Deb, A.C., (1999) Concepts of Biochemistry, 1st Edn. Books and Allied (P)
Ltd., Kolkata.
27. Collee, J.C., Duguid, J.P., Fraser, A.C. and Marimon, B.P. (1996) Mackie and
McCartney Practical Medical Microbiology, 14th Edn. Churchill
Livingstone, London.
28. Rose,A.H. (1976) Chemical Microbiology: An Introduction to Microbial
Physiology, 3rd Edn.Plenum, New York.
29. Gottschalk, G. (1986) Bacterial Mrtabolosm, 2nd Edn. Springer-Verlag, New
York.
30. Holt, J.S., Krieg, N.R., Sneath, P.H.A. and Williams, S.S.T. (1994) Bergey’s
Manual of Determinative Bacteriology, 9th Edn. Williams & Wilkins,
Baltimore.
31. Davis, B.D., Delbecco, R., Eisen, H.N. and Ginsburg, H.S. (1990)
Microbiology, 5th Edn. Harper & Row, New York.
32. Alexopolus, C.J. and Mims, C.W. (1979) Introductory Mycology, 3 rd Edn
.John Wiley and Sons, New York.
33. Schmidt, G.D. and Roberts, L.S. (1981) Foundations of Parasitology, 2nd
Edn, Mosby, St. Louis.
34. Tizard, R.I. (1983) Immunology: An Introduction. Saunders College
Publishing, Philadelphia.
35. Kuby, J. (1994) Immunology, 2nd Edn. H.W.Freeman and Company, New
York.
36. Elgert, K.D. (1996) Immunology:Understanding the Immune System. Wiley –
Liss, New York.
37. Cambell, R. (1983) Microbial Ecology, 2nd Edn. Blackwell Scientific
Publications, London.
38. Lynch, J.M. and Poole, N.J.(1979) Microbial Ecology: A. Conceptual
Approach. Blackwell Scientific Publications, London.
39. Rheinheimer, G. (1980) Aquatic Microbilogy, 2nd Edn. John Wiley & Sons,
New York.
40. Atlas, R.M. and Bartha, R. (1992) Microbial Ecology: Fundamentals and
Applications, 2nd Edn. The Benjamin / Cummings Publishing
Co.,Redwood City,CA.
41. Mitchell, R. (1974) Introduction to Environmental Microbiology. Prentice –
Hall. Inc. New Jersey.
42. Subba Rao, N.S. (1995) Soil Microorganisms and Plant Growth. Oxford &
IBH Publishing Co. Pvt. Ltd., New Delhi.
43. Paul, E.A. and Clark, F.E. (1989) Soil Microbiology and Biochemistry.
Academic Press, London.
44. Subbha Rao, N.S. (1995) Biofertilizers in Agriculture and Forestry, 3rd Edn.
Oxford & IBH Pub. Co. Pvt. Ltd., New Delhi.
45. Banwart, G.J. (1989) Basic Food Microbiology. CBS Publishers and
Distributors, New Delhi.
46. Casida, J.E. (1968) Industrial Microbiology. Wiley Eastern, New Delhi.
47. Adams, M.R. and Moss, M.O. (1995) Food Microbiology. Royal Society of
Chemistry, Cambridge.
48. Winnacker, E.L. (1987) From Genes to Clones: Introduction to Gene
Technology. VCH, Weinheim.
49. Glover, D.M. (1984) Gene Cloning:. The Mechanism of DNA Manipulation.
Chapman and Hall, London.
50. Peppler, H.J. and Pearl Man, D. (1979) Fermentation Technology, Vol 1 & 2,
Academic Press, London.
51. Brown, T.A. (1995) Gene Cloning. Chapman and Hall, London.
52. Maloy, S.R., Cronan, J.E., Jr. and David Freifelder. (1994) Microbial Genetics,
2nd Edn. Jones and Bartlett, Boston.
53. Walter Beck, J. and Davies, J.E.(1976) Medical Parasitology, 2nd Edn. C.V.
Mosby Company, St. Louis.
54. Robert Desowitz (1980) Ova and Parasites. Harper and Row Publishers, New
York.
55. Levanthal, R. and Cheadle, R.S. (1979) Medical Parasitology. S.A. Davies
Co., Philadelphia.
56. Chatterjee (1986) Medical Parasitology. Tata McGraw Hill, Calcutta.
57. Grierson, D. and Covery, S. (1989) Plant Molecular Biology, 2nd Edn.
Blackie, London.
58. Luria, S.E., Darnel, J.E., Jr., Baltimore, D. and Campbell, A. (1978) General
Virology, 3rd Edn. John Wiley & Sons, New York.
59. Fenner, F. and White, D.O. (1970) Animal Virology. Academic Press,New
York.
60. Hayes, W. (1968) The Genetics of Bacteria and their Viruses. Blackwell
Scientific Publications, London.
61. Bridge, E.A. (1994) Bacterial and Bacteriophage Genetics, 3rd Edn. Springer–
Verlag,New York.
62. Lennette, E.H. (1974) Diagnostic Procedures for Viral and Rikettsial Diseases.
American Public Health Association, New York.
63. Hoeprich, P.D. (1977) Infectious Diseases, 2nd Edn. Harper & Row
Publishers, New York.
64. Glick B.K. and Pasternak, J.J. (1999) Molecular Biotechnology. Principles and
Applications of Recombinant DNA. ASM Press, Washington, DC.
65. Stryer, L. (1995) Biochemistry. W.H. Freeman & Co., New York.
66. Ingraham, J.L. and Ingraham, C.A. (2000) Introduction to Microbiology, 2nd
Edn. Books / Cole Thomson Learning, UK.
67. Lee, J.D. (2001) Inorganic Chemistry. Blackwell Science,Oxford.
68. Sony, P.L. (2000) A Text Book of Inorganic Chemistry. S.Chand & Sons, New
Delhi.
69. Mathews, P. (1996) Advanced Chemistry.Cambridge University Press,
Cambridge.
70. Greenwood, N.N. and Earnshaw, A. (1989) Chemistry of the Elements.Mac
Millan Publication New York.
71. Cotton, F.A and Wilkinson, G. (1989) Inorganic Chemistry. John Wiley and
Sons, NewYork.
72. Finar, I.L. (1986) Organic Chemistry, Vol 1 & 2, Longman, London..
73. Schelegel, H.G. (1993) General Microbiology,7th Edn. Cambridge University
Press, Cambridge.
74. Lehninger, A.L., Nelson, D.L. and Cox, M.M. (1993) Principles of
Biochemistry,2nd Edn. CBS Publishers, New Delhi.
75. Pelczar, M.J., Jr., Chan, E.C.S and Kreig, N.R. (1993) Microbiology. McGraw
Hill, New York.
76. Negi, A.S. and Anand, S.C. (1997) A Text Book of Physical Chemistry, 5th
Edn. New Delhi.
77. Castellan (1996) Physical Chemistry. Macmillan India Ltd.,Chennai.
78. Atkins, P.W. (1997) Elements of Physical Chemistry. Oxford University
Press,Chennai.
79. Sadava, D.E.(2004) Cell Biology- organelle structure and function. Panima
Publishers Corporation, New Delhi.
80. Rastogi, S.C. (2002) Cell Biology, 2nd Edn. New Age International Publishers, New
Delhi
81. Powar, C.B. (2004) Cell Biology,3rd Edn.Himalaya Publishing House, Mumbai.
82. Rastogi, S.C.(2003) Cell and Molecular Biology. New Age International Publishers,
New Delhi
83. Gerald Karp,(2002) Cell and Molecular Biology :Concepts and Experiments,3rd Edn
John Wiley, New York.
84. Twyman, R.M.(2001) Developmental Biology- Instant Notes.Viva Books (p) Ltd.
Chennai.
85. Verma, P.S. and Agarwal, V.K. (2008) Cell Biology, Genetics, Molecular Biology,
Evolution and Ecology,5th Edn. S.Chand & Company Ltd., New Delhi.
86.Arora, M.P., Gurdarshan and Sandhu, S. (2004) Genetics,5th Edn. New Age
International Publishers, New Delhi.
87. Raven & Johnson (1990) Biology,4th Edn. Wm. C. Brown Publishers, London.
88.Pandey, S.N. and Sinha, B.K (2005) Plant Physiology,3rd Edn.Vikas Publishing
House,New Delhi
89. Mukherji, S. and Gosh, A.K.(2004) Plant Physiology .Tata McGraw Hill Publishers,
New Delhi.
91. Data, S.C. (1989) Plant Physiology. Central Book Depot, Allahabad.
93. Jacob, W.P. (1979). Plant Hormones and Plant Development. Cambridge University
Press, Cambridge.
94. Salisbury, F.B. and Ross, C.W. (1991) Plant Physiology. Wassworth Publication.
Co.. Belmont.
95. Ting, I.P. (1982) Plant Physiology. Addison Wesley Publication, Phillippines
97. Bonner, J. and Varner, J.E. (1979) Plant Physiology. Macmillan, New Delhi.
98. Daniel, W.W. (2005) Biostatistics; A foundation for analysis in the health sciences,
7th Edn. Jhon Wiley & sons Inc, New York.
99. Zar, J.H.(2006) Biostatistical analysis, 4th Edn. Pearson education Inc. New Jersey.
101. Wilson, K. and Walker, J. (2002) Practical Biochemistry: Principles & Techniques,
5th Edn. Cambridge University Press, Cambridge.
102. Sundar Rao, P.S.S. and Richard, J. (2006) Introduction to Biostatistics & Research
methods. Prentice-Hall of India(P) Ltd, New Delhi.
X. B.Sc. MICROBIOLOGY
UNIT I
and membrane protein diffusion, osmosis, ion channels, active transport, ion
pumps,
of membranes.
UNIT II
vacuoles, chloroplast, structure & function of cytoskeleton and its role in motility.
UNIT-III
euchromatin, transposons.
UNIT IV
Cell division and cell cycle: Mitosis and meiosis, their regulation, steps in cell
cycle, and
UNIT-V
stress response.
UNIT I
pathogens like bacteria, viruses into animal and plant host cells, alteration of host
diseases in animals and plants, cell-cell fusion in both normal and abnormal cells.
UNIT-II
Cell signaling: Hormones and their receptors, cell surface receptor, signaling
UNIT III
UNIT-IV
genes, cancer and the cell cycle, virus-induced cancer, metastasis, interaction of
cell growth.
UNIT V
Innate and adaptive immune system: Cells and molecules involved in innate
and cell lineages; stem cells; genomic equivalence and the cytoplasmic
UNIT-II
and germination.
UNIT III
determination.
UNIT IV
apical meristem; shoot and root development; leaf development and phyllotaxy;
and Antirrhinum.
UNIT-V
UNIT I
pathway.
UNIT-II
biosynthesis.
UNIT-IV
translocation of water, ions, solutes and macromolecules from soil, through cells,
UNIT V
SECOND SEMESTER
UNIT I
linkage and crossing over, sex linkage, sex limited and sex influenced characters.
UNIT II
Gene mapping methods: Linkage maps, tetrad analysis, mapping with molecular
in plants.
maternal inheritance.
UNIT III
analysis of genes.
Human genetics: Pedigree analysis, lod score for linkage testing, karyotypes,
genetic disorders.
Quantitative genetics: Polygenic inheritance, heritability and its measurements,
QTL
mapping.
UNIT IV
insertional mutagenesis.
UNIT V
Structural and numerical alterations of chromosomes: Deletion, duplication,
UNIT I
Principles and methods of taxonomy:Concepts of species and hierarchical taxa,
UNIT II
anatomy.
UNIT III
criteria used for classification in each taxon; classification of plants, animals and
UNIT V
UNIT I
UNIT II
UNIT III
protein and nucleotide sequence analysis; origin of new genes and proteins; gene
UNIT IV
through natural selection, migration and random genetic drift; adaptive radiation
UNIT V
UNIT I
methods; analysis of RNA, DNA and proteins by one and two dimensional gel
proteins using bacterial, animal and plant vectors; isolation of specific nucleic
cosmid, BAC and YAC vectors; in vitro mutagenesis and deletion techniques,
expression at RNA and protein level, large scale expression analysis, such as
UNIT II
UNIT III
X-ray diffraction and NMR; analysis using light scattering, different types
UNIT IV
staining techniques for EM, freeze-etch and freeze-fracture methods for EM,
UNIT V
mining methods for sequence analysis, web-based tools for sequence searches,
Objectives
enable students to build a repertoire of functional vocabulary and to move from the
lexical level to the syntactic level.
train students to summon words, phrases relevant to the immediate communication
tasks.
enable students to comprehend the concept of communication.
teach students the four basic communication skills- Listening, Speaking, Reading and
Writing.
Recommended Texts
Websites
www.tatamcgrawhill.com/digital_solutions/monippally
www.dictionary.cambridge.org
www.wordsmith.org
UOM S 003 ESSENTIALS OF SPOKEN AND PRESENTATION SKILLS
Objectives
train students to become aware of their thinking style and to enable them to convert
thinking into performance.
prepare students to evolve metal models for intra-personal and inter-personal
transactions.
make students reflect and improve their use of body language – posture, gesture, facial
expression, tone.
UNIT I: Thinking and Articulation – cognitive, affect, critical, creative aspects of articulation.
UNIT II: Acquisition of Oral and Aural Skills.
UNIT III: Communication Boosters – body language.
UNIT IV: Function of Cultural Codes in Presentation – etiquette.
UNIT V: Models of Presentation.
Recommended Texts
Objectives
Unit I- Introduction
Definition of Personality
Components of Personality – structural and functional aspects.
Determinants of Personality- biological, psychological and socio-cultural factors.
Assessment of Personality – observation, interview and psychological tests.
Misconceptions and Classifications.
Need for personality development.
PRACTICAL TRAINING
The course would include the following practical exercises.
Ice-breaking, Brainstorming and stimulation exercises. Thought stopping. Memory and study
skills training.
REFERENCES
1. Mile, D.J. (2004). Power of positive thinking. Delhi: Rohan Book Company.
2. Pravesh Kumar. (2005). All about self-motivation. New Delhi: Goodwill Publishing
House.
3. Dudley, G.A. (2004). Double your learning power. Delhi: Konark Press. Thomas
publishing Group Ltd.
4. Lorayne, H. (2004). How to develop a super power memory. Delhi: Konark Press.
Thomas publishing Group Ltd.
5. Hurlock, E.B. (2006). Personality Development, 28 th Reprint. New Delhi: Tata
McGraw Hill.
UOM S 007 COMPUTING SKILLS
Objective: The major objective in introducing the Computer Skills course is to impart training
for students in Microsoft Office which has different components like MS Word, MS Excel, MS
Access, Power point etc., at two levels based on their knowledge and exposure. It provides
essential skills for the user to get adapted to any work environment, as most of the systems in
any6 work place have MS Office installed for their day to day activities. The course is highly
practice oriented rather than regular class room teaching.
Pre-requisite: NIL.
Unit II: Word Processing – Open, Save and close word document; Editing text – tools,
formatting, bullets; Spell Checker; Navigating in word – keyword, Mouse; document
formatting – paragraph alignment, indentation, headers and footers, numbering; printing –
preview, options.
Unit III: File Management – Understanding the importance of file management; backing of
files, navigating thru My Computer and Windows Explorer; Files and Folders – editing,
retrieving, deleting, renaming, subfolders – manipulate windows – maximize, minimize; Power
point basics – terminology, templates, viewing.
Unit IV: Spreadsheets – MS Excel – opening, entering text and data, formatting, navigating;
Formulas – entering, handling and copying; Charts – creating, formatting and printing, header
and footer, centering data, printing.
References:
1. Introduction to Computers – Peter Norton, Tata McGraw-Hill.
1. Microsoft 2003 – Jennifer Ackerman Kettel, Guy Hat-Davis, Curt Simmons, Tata
McGraw-Hill.
Examination:
1. Internal assessment could be based on Theory and/or practicals.
2. End semester is based on practicals.