Course Course Course L T P C

18BTC106J IMMUNOLOGY C Professional Core

Code Name Category 3 0 2 4

Pre-requisite Co-requisite Progressive

Nil Nil Nil
Courses Courses Courses
Course Offering Department Biotechnology Data Book / Codes/Standards Nil

Course Learning Rationale (CLR): The purpose of learning this course is to: Learning Program Learning Outcomes (PLO)
CLR-1 : Examine the science of immunology and a detailed study of various types of immune cells 1 2 3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
CLR-2 : Distinguish immune systems produced molecules and their classification, structure and function

Environment & Sustainability

Analysis, Design, Research
Level of Thinking (Bloom)

Expected Proficiency (%)

Expected Attainment (%)

Engineering Knowledge

Individual & Team Work

CLR-3 : Choose methods used in immunology, particularly the use of specific antibody in bio-molecular applications

Design & Development

Project Mgt. & Finance

Modern Tool Usage

Life Long Learning

Society & Culture
Problem Analysis
CLR-4 : Evaluate knowledge about immune system, their cells, its interaction and how they fight against infectious diseases

Communication

PSO – 3
CLR-5 : Analyze the dysregulation of immune system functioning and ways to strengthen immune system

PSO - 1

PSO - 2
Ethics
CLR-6 : Evaluate the knowledge about how human body is designed and protected to fight against various pathogens

Course Learning Outcomes

At the end of this course, learners will be able to:
(CLO):
CLO-1 : Describe the immune system and their structure and classification 1 80 70 M - H H L L L H - H M H M H H
CLO-2 : Discuss about genetic control of antibody production, cellular immunology 2 80 70 M M - H H M H H - H M H M H H
CLO-3 : Explain various methods to assess immune function, their application and interpretation of the results 2 80 70 M M L H H - - H M H M H H H H
CLO-4 : Describe the role of the immune molecules in infectious diseases, autoimmunity, and cancer will be discussed 2 80 70 - - - H H M H H M H M L H H H
CLO-5 : Discuss about hypersensitive immune reaction, vaccination and cancer immunology 2 80 70 M M - H H - H H M H H M H H H
Describe how immune cells, organ and processes function to protect human body against infective agents and cancer
CLO-6 : 2 80 70 M L M H H M M H M H H M H H H
cells.

Duration (hour) 15 15 15 15 15
Isolation of immune cells from Human and
SLO-1 Overview of the immune system Immunoglobulin structure Major histo-compatibility Complex(MHC) Hypersensitive reactions
animals
S-1
Development and differentiation of the
SLO-2 Immunoglobulin types and function Antigen- antibody interaction MHC – types and function Type I and Type II reaction
hematopoietic stem cells
Antibodies biological and functional
SLO-1 Myeloid and Lymphoid lineage antibody affinity and avidity MHC Class I Type III and Type IV reaction
properties
S-2
Immune responses to infectious diseases
SLO-2 Lymphatic system Proteolytic digestion of antibodies Hemaagglutination reaction MHC Class II
introduction
antigen processing and presentations –
SLO-1 Lymphoid organs - types Monoclonal antibodies production Coombs test – direct and indirect Viral disease-HIV infection
Endogenous and Exogenous
S-3
SLO-2 Innate lymphoid cells Monoclonal antibodies applications precipitation reaction Diversity of MHC molecules Bacterial disease-Tuberculosis
S SLO-1 Lab 1:Laboratory safety principles and Lab 4: Antigen – Antibody reaction I – Lab 10: Active immunodiffusion – II – Lab 13: Enzyme linked Immunosorbent
Lab 7: Ouchterlony gel diffusion
4-5 SLO-2 Blood grouping Widal test Counter Current Immunoelectrophoresis assay (ELISA) – DOT
Agglutination principle, blood group types Widal test - slide method and test tube Types of ELISA, Direct vs Indirect ELISA,
SLO-1 Single radial immunodiffusion (SRID) Antigen – Antibody interaction
Rhesus group types method Dot ELISA Sandwich ELISA
S-6
incompatible blood transfusion and titer value, zone of equivalence Standard and test antigen
SLO-2 B Cell differentiation Parasitic disease-Malaria
hemolytic disease Quantitative Immuno assays Rocket Immunoelectrophoresis
B cell receptor structure and B cell signal
SLO-1 Receptors of Innate Immune system passive Immunodiffusion Biology of T lymphocyte Evading Mechanisms of pathogens
transduction
S-7
SLO-2 Types of Immune cells, Innate Immunity Antibody diversity Precipitation reaction T cell receptors and interaction with MHC Vaccine history and principle
Active Immunodiffusion – Rocket
SLO-1 Anatomical and Physiological barriers Light chain synthesis T-cell maturation Active and passive Immunization
immunoelectrophoresis
S-8
Heavy chain synthesis Cytokine receptor DNA vaccine, Edible vaccine and
SLO-2 Acquired Immunity, clonal selection theory SDS-PAGE and Western blot T-cell activation and differentiation
structure Adjuvants
 S SLO-1 Lab 5: Antigen – Antibody reaction II -rapid Lab 14: Enzyme linked Immunosorbent
Lab 2: Total Leukocyte count Lab 8: Repeat/Revision of experiments Lab 11: Immunoprecipitation
9-10 SLO-2 plasma reagin (RPR) test assay (ELISA) – Plate
Types of blood cells Flocculation reaction Quantitative Immuno assays - Radio- Thymic selection – Positive and negative
SLO-1 Tumor Immunology introduction
Leukocyte counting Rapid Plasma Reagin (RPR) test immunoassay selection
S-11
Comparative immunity - Plant Immune
SLO-2 Cytokine types and function Precipitation reaction, Immunoprecipitation T-cell activation and cytokine secretion Evidence for Tumor Immunity
system
Vertebrate and Invertebrate Immune Result interpretation
SLO-1 Role of cytokines in diseases Immunofluorescence – Direct and indirect Tumor immuno therapy
system Counter current immuno electrophoresis
S-12
SLO-2 Immunogens, Antigens and Haptens Complement system Immunohistochemistry Cytokine control of TH1 and TH2 CD4+ Autoimmunity introduction
Requirements for immunogenicity; major Function of CD8+ T cells, T Regulatory
SLO-1 Regulation of complement pathway flow cytometry, ELISA and types Genetic Basis of Autoimmunity
classes of antigens cells
S-13
antigen recognition by T and B Cell culture and experimental models, T-cell and B-cell cooperation, Pathways of Classification of auto-immunity
SLO-2 Role of complement proteins in diseases
lymphocytes analysis of gene expression Activation
S SLO-1
Lab 6: Single radial immunodiffusion Lab 9: Active Immunodiffusion I - Rocket
14- Lab 3: Differential Leukocyte count Lab 12: SDS-PAGE Lab 15: Western blotting
SLO-2 (SRID) Immunoelectrophoresis
15

Learning
1. Sudha Gangal, Shubhangi Sontakke, Textbook of basic and clinical immunology, Universities Press, 2013 2. Jenni Punt, Sharon Stranford, Patricia Jones, Judith A Owen, Kuby Immunology, 8 th ed., W.
Resources H. Freeman and Company, 2018

Learning Assessment
Continuous Learning Assessment (50% weightage)
Bloom’s Final Examination (50% weightage)
CLA – 1 (10%) CLA – 2 (15%) CLA – 3 (15%) CLA – 4 (10%)#
Level of Thinking
Theory Practice Theory Practice Theory Practice Theory Practice Theory Practice
Remember
Level 1 20% 20% 15% 15% 15% 15% 15% 15% 15% 15%
Understand
Apply
Level 2 20% 20% 20% 20% 20% 20% 20% 20% 20% 20%
Analyze
Evaluate
Level 3 10% 10% 15% 15% 15% 15% 15% 15% 15% 15%
Create
Total 100 % 100 % 100 % 100 % 100 %
# CLA – 4 can be from any combination of these: Assignments, Seminars, Tech Talks, Mini-Projects, Case-Studies, Self-Study, MOOCs, Certifications, Conf. Paper etc.,

Course Designers
Experts from Industry Experts from Higher Technical Institutions Internal Experts
1. Dr. C. N. Ramchand, Saksin Life sciences Pvt Ltd, Chennai, ramchand@saksinlife.com 1. Dr. Joe Varghese, CMC Vellore, joevarghese@cmcvellore.ac.in 1. Dr. S. Thyagarajan, SRMIST
2. Dr. Karthik Periyasamy, Aurobindo Pharma Limited, Hyderabad, karthikmpk@gmail.com 2. Prof. K Subramaniam, IITM, Chennai, subbu@iitm.ac.in 2. Dr. S. Nageswaran, SRMIST