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Enzyme Assay Methods
Enzyme Assay Methods
Enzyme Assay Methods
Manometric
Electrode method
method
Spectrophotometric methods
• Many substrates and products of enzyme reactions absorb
light either in the visible region or in the U.V. region.
These is easily
followed
NAD+ NADH
Oxidized form Reduced form Absorbs
at 340nm
NADP+ NADPH
D-glucose L-glucose
Cu+2
Cu+2 reduction
(colour change)
α-amylase
Starch + H2O glucose (reducing sugar)
Cu+2
Pyruvate
2,4 DNPH
2,4 dinitrophenyl
Hydrazone NaOH
(Brown colour) (alkaline pH)