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Enzymeimmobilization PDF
Enzymeimmobilization PDF
Enzymeimmobilization PDF
IMMOBILIZATION
• The enzyme in turn may be bound to another enzyme via cross linking.
Disadvantages:
• Not fit for industrial use as Effectiveness gradually
decreases.
• Ionic interactions between substrate and enzyme
disturbs the enzyme activity. Substrate specificity is
unchangeable.
CROSS LINKING
• This method is based on the
formation of covalent bonds
between the enzyme
molecules, by means of
multifunctional reagents,
leading to three dimensional
cross linked aggregates.
• Disadvantages of entrapment:-
– The enzyme can leak into the surrounding medium.
– Another problem is the mass transfer resistance to
substrates and products.
– Substrate cannot diffuse deep into the gel matrix.
REFERENCES
• http://enzymeimmobilization.blogspot.in/2011/02/enzyme-
immobilization.html
• https://en.wikipedia.org/wiki/Immobilized_enzyme
• https://www.google.co.in/search?q=enzyme+immobilization&espv
=2&biw=1600&bih=766&source=lnms&tbm=isch&sa=X&ved=0CAg
Q_AUoAmoVChMIxuqanKDOxwIVE3GOCh3ulQif