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Plasmid Files

Home » Resources » Plasmid Files » Yeast Plasmids » pPIC9K

pPIC9K
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Pichia pastoris multi-copy integrating vector for methanol-inducible  expression of a secreted
protein.
 
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Yeast Plasmids
pPIC9K

Plasmid Sets 
pPIC9K.dna
Map and Sequence File:    Download    Open    
Sequence Author:  Thermo Fisher (Invitrogen)

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Show Static Map

(9101) AatII AleI (76)

(9099) ZraI Eco53kI (206)
SacI (208)
(8659) ScaI PmeI (413)
BlpI (588)
XcmI (706)
BamHI (938)
PsiI (1144)
SnaBI (1218)
EcoRI (1222)
AvrII (1228)
MCS
EagI - NotI (1235)
AgeI (1300)
BtgZI (1754)
FspAI (1826)

(7286) AflIII - PciI

XbaI (2032)
(7170) BspQI - SapI Bsu36I (2088)
(7109) NdeI HpaI (2138)
(7059) BstZ17I
(7033) PflFI - Tth111I pPIC9K
9276 bp

SalI (3177)
(6036) SphI

(5880) SgrAI
(5709) PspXI
(5654) NruI NcoI (3632)
(5437)SmaI
(5435) TspMI - XmaI BspEI (3844)
(5313) AsiSI

BsrGI (4298)
(4644) BbvCI

5ʹ AGATCTAACATCCAAAGACGAAAGGTTGAATGAAACCTTTTTGCCATCCGACATCCACAGGTCCATTCTC
70

3ʹ TCTAGATTGTAGGTTTCTGCTTTCCAACTTACTTTGGAAAAACGGTAGGCTGTAGGTGTCCAGGTAAGAG
AOX1 promoter

AleI

ACACATAAGTGCCAAACGCAACAGGAGGGGATACACTAGCAGCAGACCGTTGCAAACGCAGGACCTCCAC
140
TGTGTATTCACGGTTTGCGTTGTCCTCCCCTATGTGATCGTCGTCTGGCAACGTTTGCGTCCTGGAGGTG
AOX1 promoter

Eco53kI SacI

TCCTCTTCTCCTCAACACCCACTTTTGCCATCGAAAAACCAGCCCAGTTATTGGGCTTGATTGGAGCTCG
210
AGGAGAAGAGGAGTTGTGGGTGAAAACGGTAGCTTTTTGGTCGGGTCAATAACCCGAACTAACCTCGAGC
AOX1 promoter

CTCATTCCAATTCCTTCTATTAGGCTACTAACACCATGACTTTATTAGCCTGTCTATCCTGGCCCCCCTG
280
GAGTAAGGTTAAGGAAGATAATCCGATGATTGTGGTACTGAAATAATCGGACAGATAGGACCGGGGGGAC
AOX1 promoter

GCGAGGTTCATGTTTGTTTATTTCCGAATGCAACAAGCTCCGCATTACACCCGAACATCACTCCAGATGA
350
CGCTCCAAGTACAAACAAATAAAGGCTTACGTTGTTCGAGGCGTAATGTGGGCTTGTAGTGAGGTCTACT
AOX1 promoter

PmeI

GGGCTTTCTGAGTGTGGGGTCAAATAGTTTCATGTTCCCCAAATGGCCCAAAACTGACAGTTTAAACGCT
420
CCCGAAAGACTCACACCCCAGTTTATCAAAGTACAAGGGGTTTACCGGGTTTTGACTGTCAAATTTGCGA
AOX1 promoter

Individual Sequences & Maps

α-factor pFA6a- pGBD-C3 pRS313 YCp50-poly
secretion GFP(S65T)- pGBDU-C1 pRS314 YCplac22
signal kanMX6 pGBDU-C2 pRS315 YCplac33
bleMX6 pFA6a- pGBDU-C3 pRS316 YCplac111
hphMX6 GFP(S65T)- pGBKT7 pRS403 YEp13
kanMX TRP1 pGilda pRS404 YEp24
kanMX2 pFA6a-GST- pHIL-D2 pRS405 YEp351
kanMX4 His3MX6 pHIL-S1 pRS406 YEp352
kanMX6 pFA6a-GST- pJG4-5 pRS413 YEp353
natMX6 kanMX6 pKLAC1 pRS414 YEp354
pAbAi pFA6a-GST- pKLAC2 pRS415 YEp355
pAD-GAL4 TRP1 pOAD pRS416 YEp356
pAG25 pFA6a-His3MX6 pOBD2 pRS423 YEp356R
pAG29 pFA6a- pP-secSUMO3 pRS424 YEp357
pAG32 His3MX6- pPIC3 pRS425 YEp357R
pAO815 PGAL1 pPIC3.5 pRS426 YEp358
patMX4 pFA6a- pPIC3.5K pTEF1/Zeo YEp358R
pAUR101 His3MX6- pPIC6α A pUCATPH YEp363
pAUR112 PGAL1-3HA pPIC6α B pY- YEp364
pAUR123 pFA6a- pPIC6α C secSUMOstar YEp365
pAUR224 His3MX6- pPIC6 A pY-SUMOstar YEp366
pBridge PGAL1-GFP pPIC6 B pYC2-E YEp366R
pDH25 pFA6a- pPIC6 C pYC2/CT YEp367
pEG202 His3MX6- pPIC9 pYC2/NT A YEp367R
pESC-HIS PGAL1-GST pPIC9K pYC2/NT B YEp368
pESC-LEU pFA6a-hphMX6 pPICZα A pYC2/NT C YEp368R
pESC-TRP pFA6a-kanMX6 pPICZα B pYC6/CT YEplac112
pESC-URA pFA6a-kanMX6- pPICZα C pYES2 YEplac181
pFA6-kanMX4 PGAL1 pPICZ A pYES2.1-E YEplac195
pFA6a pFA6a-kanMX6- pPICZ B pYES2/CT YIp5
pFA6a-3HA- PGAL1-3HA pPICZ C pYES2/NT A YIp351
His3MX6 pFA6a-kanMX6- pPINKα-HC pYES2/NT B YIp352
pFA6a-3HA- PGAL1-GFP pPink-HC pYES2/NT C YIplac128
kanMX6 pFA6a-kanMX6- pPink-LC pYES3/CT YIplac204
pFA6a-3HA- PGAL1-GST pRS303 pYES6/CT YIplac211
TRP1 pFA6a-natMX6 pRS304 pYM25 YRp7
pFA6a-5FLAG- pFA6a-TRP1 pRS305 pYM26
kanMX6 pFA6a-TRP1- pRS306 pYM44
pFA6a-12Pk- PGAL1 YCp50
kanMX6 pFA6a-TRP1-
pFA6a-13Myc- PGAL1-3HA
His3MX6 pFA6a-TRP1-
pFA6a-13Myc- PGAL1-GFP
kanMX6 pFA6a-TRP1-
pFA6a-13Myc- PGAL1-GST
TRP1 pFLD
pFA6a-bleMX6 pFLDα
pFA6a- pGAD-C1
GFP(S65T)- pGAD-C2
His3MX6 pGAD-C3
pGADT7-Rec
pGADT7-Rec
(linearized)
pGADT7 AD
pGAPZα A
pGAPZα B
pGAPZα C
pGAPZ A
pGAPZ B
pGAPZ C
pGBD-C1
pGBD-C2

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material.

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