Document ID: Title Print Date

A02182020-0022 Sodium Nitrite 03/02/2020

Revision Prepared by Date Prepared
01 Andrea Rei G. Baldeo 02/18/2020
Effective Date Received by Date Received
Gerlaine Pasion
Analyst 2
Approved by Date Approved
Jacqueline Padilla

Policy:

1) Dress for the Lab. This is a safety rule because your clothing is one of your best forms

of protection against an accident. For any science lab, wear covered shoes, long pants, and keep

your hair up so it can't fall into your experiment or a flame.Make sure you wear protective gear

as needed. Basics include a lab coat and safety goggles. You may also need gloves, hearing

protection, and other items, depending on the nature of the experiment.

2) Know the Location of Safety Equipment. It's a good idea to periodically check

equipment to make sure it is in working order. For example, does water actually come out of the

safety shower? Does the water in the eye wash look clean?

3) Know the Procedures and How to Use the Equipment. Knowing what to do during the

experiment is a critical part. Be sure to follow the instructions of your teacher before starting any

of the procedure to avoid unwanted accidents.

4) Don't eat or drink in the science laboratory. Don't store your food or beverages in the

same refrigerator that contains experiments, chemicals, or cultures.

 5) Dispose of Lab Waste Properly. Before you start an experiment, you should know

what to do at the end. Don't leave your mess for the next person to clean up.

• Are the chemicals safe to dump down the drain? If not, what do you do with them?

• If you have biological cultures, is it safe to clean up with soap and water or do you need

an autoclave to kill dangerous organisms?

• Do you have broken glass or needles? Know the protocol for disposing of "sharps".

Purpose:

To determine the concentration of Sodium Nitrite according to its labelled claim.

Scope:

 Determination of the concentration of Sodium Nitrite.

Person Responsible:

 Pharmaceutical Analyst Head, Analyst 1, Analyst 2, and Quality Assurance Manager

5. Standard Operating Procedures

ASSAY

 Procedure

Sample solution: Dissolve 0.400 g of sodium nitrite in 100.0 ml of water. Introduce 200

ml of the solution, while stirring continuously and keeping the tip of the pipette below the

surface of the liquid, into conical flask containing 30.0 ml of 0,1 M cerium sulphate.

Immediately stopper the flask and allow standing for 2 min.

Analysis: Add 10 ml of a 200 g/l solution of potassium iodide and 2 ml of starch

solution. While stirring continuously, titrate with 0.7 M sodium thiosulphate until the

blue colour disappears. Carry out a blank titration.

Acceptance Criteria: 98.5% to 100.5%