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NIMBUS IVD Operators Manual - EN
NIMBUS IVD Operators Manual - EN
MICROLAB NIMBUS
IVD
Operator’s Manual
Part Number 65517-01 (Rev. A)
November, 2011
Important Notice
• Reproduction of any part of this manual in any form whatsoever without the express
written consent of Hamilton Company is forbidden.
• The contents of this manual are subject to change without notice.
• All efforts have been made to ensure the accuracy of the contents of this manual.
However, should any errors be detected, Hamilton Company would greatly appreciate
being informed of them.
• The above notwithstanding, Hamilton Company can assume no responsibility for any
errors in this manual or their consequences.
• Copyright © 2011 Hamilton Company. All rights reserved.
ii Contents
Contents
Contents iii
2.8 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-25
2.8.1 Hamilton Heater Shaker (HHS) . . . . . . . . . . . . . . . . . . . . . . . . . . 2-25
2.8.2 Liquid Waste System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-26
iv Contents
Chapter 7 — Technical Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-1
7.1 Basic Instrument NIMBUS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-1
7.1 Shift-N-Scan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-3
7.1.1 Supported Symbologies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-3
7.1.2 Reading Accuracy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-3
7.1.3 Barcode Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-4
7.1.4 Positioning Sample Barcodes . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-5
7.2 CO-RE Gripper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-6
7.3 UV Lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-6
Glossary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . GL-1
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . IN-1
Contents v
MICROLAB® NIMBUS IVD Operator’s Manual (Rev. A)
Chapter 1
General Information
1.1.1 Symbols
The “Biohazard” symbol alerts the operator to situations where special care is required to remain
protected from chemical or biochemical hazards.
The “Warning!” symbol contains information that must be followed to prevent personal injury to those
operating the equipment.
The “Important” symbol gives instructions that must be followed to prevent damage to equipment or
loss of data.
The “Note” symbol provides useful information to improve system performance or directs you to
supplemental information to improve your understanding of overall operations.
Read the following safety notices carefully before using the NIMBUS.
1.4.1 General
Precautions
1.4.1.1 Instrument
During operation, the NIMBUS must be shielded from direct sunlight and
intense artificial light. The instrument should be positioned in the laboratory
in a way permitting personnel to access the front and sides of the instrument
for operation, maintenance, and opening and removal of protective covers.
Accordingly, to calculate how much room is needed, consider the dimensions
of the instrument (See Chapter 7 “Technical Specifications” on page 7-1) and
sufficient room for a person to move and work comfortably.
Never lift a fully installed instrument to transport it from one place to another.
It must be re-installed in the new work location by an authorized service
technician only.
The instrument weighs more than 225 lbs (102 kg). Necessary precautions
should be taken when transporting the instrument.
Only certified technicians are authorized to perform mechanical maintenance
on the NIMBUS.
For repair or shipment, all mechanical parts must be put in their rest
positions. A NIMBUS sent away for repair must also be decontaminated
(see “Decontamination” section on page 5-9) if it was in a laboratory
environment with infectious or hazardous materials.
The NIMBUS must be repacked in the original shipping crate only by an
authorized service technician (contact your local HAMILTON
representative). There must be no containers or tips on the NIMBUS during
transportation.
The service technician and the laboratory share the responsibility for the
installation qualification (IQ) and the operation qualification (OQ), i.e.
verification and training. The process qualification (PQ) is the sole
responsibility of the laboratory.
Only original HAMILTON NIMBUS specific parts and tools may be used
with the NIMBUS, e.g. pedestals, racks, CO-RE tips, and waste containers.
Commercially available liquid containers, such as microtiter plates and tubes,
may of course be used.
An interruption of power during a run may cause the loss of data. If data loss
is unacceptable, use an independent power supply or an Uninterruptible
Power Supply (UPS).
Any persons operating the NIMBUS and the PC running the NIMBUS
software must have attended a certified training course. Any departure from
the procedures given in this manual and during training could lead to
erroneous results or NIMBUS malfunction.
1.4.1.3 Method
Programming
Programming and validation of new methods is the responsibility of every
customer.
Perform test runs first with water and then with the final liquids, prior to
routine use. Test all the liquid classes you are going to use. A newly
programmed test method must first be run on the NIMBUS with the final
liquids, prior to validation of the method and routine use. The method
programmer should supervise this run.
Do not use methods which are not validated! Before using any newly created
or modified method for routine test purposes, a comparison study between the
method previously used and the new one must be carried out by the laboratory
supervisor to ensure that the processing and data evaluation of both methods
produce equal results.
When working with samples, which will be used in particularly sensitive
tests, take into account the evaporation and condensation that may occur
while the method is running.
If sampling aggressive liquids, use filter tips. Also use filter tips for tasks
which are sensitive to cross-contamination (aerosols).
Liquid level detection needs to be explicitly tested when working with
foaming liquids. Foam may affect the accuracy of liquid level detection.
Never disable any security measure.
1.4.1.4 Loading
Microplates must be placed such that well A1 is in the position defined in the
deck layout of the method.
When pouring liquid into the containers, ensure that there is no foam on the
surface of the liquid. Note that foam may cause pipetting problems.
Do not overfill reagent containers, tubes or other liquid containers.
Do not mix tip size and type (e.g. with or without filter or different volumes)
in the same tip rack.
Do not fill up tip racks with tips from another rack. Tips should be loaded in
the tip racks as they are provided in the original packaging. They are
individually labeled for identification.
1.4.2 Biohazard
Precautions
If the NIMBUS becomes contaminated with biohazardous or chemical
material, it should be cleaned in accordance with the maintenance procedures.
See Chapter 5 “Maintenance” on page 5-1. Observe and carry out the
maintenance procedures given. Failure to do so may impair the reliability and
correct functioning of the NIMBUS.
If working with biohazardous samples, observe and carry out the maintenance
procedures, paying particular attention to cleaning and decontamination.
Wear gloves when handling the pipetting arm and channels, the pedestals, and
the tips. Avoid touching tips discarded into waste container. Any surfaces on
which liquid is spilled must be decontaminated.
If working with biohazardous or chemical materials, the user must not touch
them.
The NIMBUS will drop its used tips into a waste container that should be
emptied during the daily maintenance or as soon as it is full.
1.4.3 Computer
Precautions
Use the necessary precaution to guard against software viruses. Use only
manufacturer’s original installation DVD/CD-ROM sets for the operating
system and the original NIMBUS software.
Do not return instruments to Hamilton Company without an RMA number. This number assures proper
tracking of your instrument. Instruments that are returned without an RMA number will be sent back to
the customer.
Hamilton Company reserves the right to refuse a return shipment of any Hamilton product that has
been used with radioactive, microbiological substances, or any other material that could be
hazardous to Hamilton employees.
Chapter 2
Description of the NIMBUS
Below are the standard and optional pipetting features of the NIMBUS.
Figure 2-2: How the NIMBUS uses the CO-RE technology to pick up tips.
The advantages of the CO-RE technology include the ability to use disposable
tips of various sizes as well as the use of various tools, all in the same run.
2.1.3 Tip-On
Recognition
The NIMBUS can detect when a tip is attached and when it is not. When a tip
is picked up, the outer sleeve of the pipette head moves upward, triggering a
sensor. When the tip is ejected, the spring-loaded sleeve moves downward,
and the sensor indicates the tip has been released. This security feature
prevents the accidental aspiration of liquid without a tip attached to the pipette
head.
The NIMBUS cannot differentiate between 300 µl and 50 µl tips, or between filtered and non-filtered
tips. When placing these kinds of tips onto the deck, pay special attention to their designated
locations.
2.1.8 Aspiration
Monitoring with cLLD
This mode is used to detect when the tip is no longer within the liquid. If this
mode is activated and cLLD is used during the aspiration, the capacitive
signal is monitored throughout the aspiration. If the signal changes abruptly,
the system will indicates that the tip is out of the liquid
2.1.9 Anti-Droplet
Control (ADC)
Pipetting volatile liquids can be a challenge. The liquid inside the tip can
evaporate, causing an increase in pressure and a resulting formation of a
droplet. The Anti-Droplet Control (ADC) mode can be turned on to
compensate for that increased pressure. As the pressure increases, the plunger
pulls back slightly, reducing the inner pressure and preventing the droplet
from forming.
Figure 2-8 shows NIMBUS as it would the sit on the bench or cart.
Figures 2-9, 2-10 and 2-11 point out the parts of the NIMBUS IVD base
instrument.
The Gantry (Figure 2-9) is the arm that houses the independent channels. It
moves left and right across the deck of the NIMBUS.
The lower front side of the NIMBUS has a blue display light which indicates
the status of the Gantry itself. See Table 2-1and Figure 2-9.
The front door of the NIMBUS enclosure has a handle for opening and
closing the door (Figure 2-9).
On the right side of the NIMBUS is the instrument’s power switch, power
port and communication port (Figure 2-10 and Figure 2-11).
Remove the power panel cover to get access to the power switch and ports.
2.4.1 Channels
The NIMBUS is configured with up to 4 independent pipetting channels.
Each channel incorporates a pipetting head. Two pipetting head sizes are
available: 1 ml and 5 ml. A NIMBUS can have one of the following
configurations:
• Up to four 1 ml channels
• Up to two 5 ml channels
Mixing the channel types is not supported at this time.
The channels have independent spacing. That is, there is no fixed maximum
spacing between channels. For example, three 1 ml channels can be
pipetting in the front of the NIMBUS while the fourth channel is pipetting
in the very rear of the NIMBUS. The minimum spacing between 1 ml
channels is 18 mm. The minimum spacing between 5 ml channels is 36 mm.
The 1 ml channels support the use of 10 µl, 50 µl, 300 µl, and 1000 µl filtered
and non-filtered disposable CO-RE tips. The 5ml channels support the use of
5 ml unfiltered and 4 ml filtered disposable CO-RE tips.
2.4.2 Decks
The NIMBUS can be configured with one of three deck configurations. The
options are:
• 9+2 deck
• 3x4 deck
• Shift-N-Scan deck
The 9+2 deck (Figure 2-13) has 11 positions, 9 of which are on the main
portion of the deck, in a 3x3 configuration. The other 2 positions are on the
left side of the deck. This portion of the deck can be adjusted to one of 5 levels
in the Z direction. The height-adjustable portion of the 9+2 deck can be
lowered in 12 mm increments, down to 48 mm below the level of the main
deck.
The 3x4 deck has 12 positions, all on one level (Figure 2-14).
The Shift-N-Scan deck has a 2x4 array of positions plus the Shift-N-Scan
device for holding up to 96 barcoded tubes. Everything is on one level. The
Shift-N-Scan device is affixed to the front three deck sites (shown as SS1-SS3
in Figure 2-15).
Barcoded tubes are placed into the device. A barcode reader is affixed to the
Gantry. The Gantry moves in the X direction to read the barcodes.
The Shift-N-Scan has special racks (Figure 2-16). Each row of these racks
moves independently. When a row of tubes is to be read, its row shifts slightly
in X, to a position that allows the reader to scan those barcodes through slots
between the tubes in the other rows.
The back wall of the Shift-N-Scan device has a series of barcode labels. If a
tube is missing from the row that is being scanned, the reader will see the
barcode label on the back wall. This special barcode indicates to the NIMBUS
that an expected tube is missing.
For all deck types, the maximum height of any item on the deck is 118 mm above the main deck
surface. Items that sit higher than that are in the “no-fly zone.” That is, the tips could collide with those
tall items and cause a crash. For tall labware items, consider using the two height-adjustable deck
sites on the 9+2 deck. The maximum height of items on those lowered deck sites is 166 mm.
Plate transport with CO-RE Grippers is only allowed within the deck area.
Also, plate rotation is not possible with the CO-RE Grippers.
The lamps are fitted with an integrated safety shield that protects the user
from mercury and broken quartz in the event of accidental breakage. This
protective coating provides excellent transmission of ultraviolet energy, with
minimal loss in output.
The covers of the enclosed NIMBUS are made of polycarbonate. The covers
prevent the transmission of UV energy to the outside of the NIMBUS.
Never disable any safety measure. Always operate the NIMBUS with the door closed. Exposure to
the lamps' (UV-C) energy is a hazard to skin and eyes.
It is the responsibility of the laboratory to verify the NIMBUS UV Lamp output meets the laboratory's
requirements for decontamination.
The lamps contain mercury. Manage them in accordance with local disposal laws.
After 400 hours of use, the UV lamp intensity degrades to some level. Each laboratory should
continue to verify - throughout the life of the lamps - that the intensity meets the laboratory's
requirements for decontamination.
2.5 Pedestals
The NIMBUS utilizes specially-designed blocks to hold various labware in
precise positions on the deck. These pedestals are easily placed onto, removed
from, or moved around the deck in order to customize each application. This
section describes the various pedestals available as of the date of this manual.
A single NTR rack can sit on an NTR pedestal on the main portion of the 9+2
deck or the other available decks. Stacked NTRs are not allowed on the main
portion of any deck, as they sit higher than the traverse height of the NIMBUS
channels.
The FTR pedestal holds tips in frames. “FTR” stands for Filtered Tip Racks,
but this pedestal holds both filtered and unfiltered tips that are in frames. The
latches on either side of the frame snap into the locks on the FTR pedestal.
For PCR plates, there are two PCR plate adapters available. There is an
adapter for 96-well plates and one for 384-well plates. A PCR plate adapter
does not stand alone as its own pedestal; it sits on top of a DWP pedestal.
The 96- and 384-well PCR adapters are for skirted and unskirted PCR plates
and for PCR strip-wells (Figure 2-21).
For 0.5 to 2 ml vials, there is the Small Tube Adapter. This adapter sits on top
of an NTR pedestal. The adapter holds up to 32 vials. See Figure 2-24.
The tip type is printed in plain text on the barcode label of the tip rack for visual identification. For
example, “50 ul” for the 50 µl CO-RE tips.
Before running a NIMBUS, verify the right tips are in the correct location. Picking up the wrong tips
could cause poor pipetting result or pipetting liquid into the head (which could damage it).
When transitioning from one tip type* to another, it is important to re-evaluate pipetting performance
and if necessary, adjust liquid class settings to achieve optimal pipetting performance.
* tip types: size (10 µl vs. 50 µl, 300 µl, 1000 µl or 5000 µl); color (black vs. clear); filters (non-filtered
vs. filtered; sterility (sterile vs. non-sterile).
The 10 µl, 50 µl and 300 µl unfiltered tips are stackable. Larger tips, or tips
that are filtered, are not stackable.
Disposable tips for the NIMBUS are only available through Hamilton
Company.
NTRs require the use of the CO-RE Grippers. After a rack of tips is
consumed, the gripper will pick up the empty rack and put it into a stack of
empty racks or into the waste bin.
NTRs come in a package of 5 stacks of 4 racks each. NTRs in stacks can only
be placed onto the lowered portion of the 9+2 deck.
A stack of NTRs on the main deck will be too tall for the NIMBUS's traverse height and will potentially
cause a crash.
Attached to the waste block is the Tip Eject Plate. This plate is intended to be
removed by the operator for cleaning and during teaching or maintenance
modes. The plate is easily removed; just pull up and away from the waste
block.
Wear gloves when removing the tip eject plate, as it could be contaminated.
Under the tip eject plate are the 1 ml Teaching Needles. On a NIMBUS
configured with 1ml channels, these needles are used for daily maintenance,
calibration, teaching labware positions, and for some service activities.
The 5 ml Teaching Needle is located in a position just in front of the tip eject
plate. On a NIMBUS configured with 5 ml channels, it is used for daily
maintenance, calibration, teaching labware positions, and for some service
activities.
To the right of the waste block is the Waste Bin. The waste bin holds the
ejected tips. This bin is meant to be lined with a waste bag. When the
NIMBUS is idle, the operator can remove the waste bin. The waste bin uses a
frame to hold the waste bag in place.
To the rear of the tip eject plate is a place for the optional liquid waste tub. If
equipped with the liquid waste system, the tub drains to a carboy that has a
level sensor. The liquid waste system is a good option for extraction
applications.
Finally, the waste block has positions for the optional CO-RE Grippers.
The CO-RE Grippers come with park position brackets. These brackets
attach to the waste block and are adjustable. The brackets have magnets,
as do the CO-RE Gripper paddles. The magnets hold the paddles in place.
2.8 Accessories
A variety of accessories and other devices are available for use on the
NIMBUS. This section describes the most common of these. Many of these
items will come with their own documentation describing their installation
and use.
It is not recommended to locate the HHS immediately next to the waste block, as it is difficult to attach
the integration plate to the deck at those locations.
Chapter 3
Setting up the NIMBUS
2. Connect one end of the Ethernet cable into the Ethernet port of the
NIMBUS. Connect the other end into the network interface port in
the NIMBUS computer.
4. There are two level shelf assemblies on the deck assembly (Figure 3-4).
Remove the two socket-head screws from each of the level shelf
assemblies, using an Allen wrench.
The left side deck can be moved up or down at 12 mm increments. There are 5 possible positions
relative to the main deck: 0, -12, -24, -36, and -48 mm.
6. Carefully replace the left side deck onto the two level shelf assemblies.
7. Install the four button-head screws into the left side deck, using an
Allen wrench (refer to Figure 3-3).
3. Place the waste bin behind the waste station as shown in Figure 3-7.
You may find that the bag fits better if you cut off a portion from the top of the bag before placing it
into the waste bin.
Where screen shots are provided in this manual, they are taken from revision B of the NIMBUS
Independent Channel software.
Once the software is installed, you will see up to 6 new icons on the computer
desktop:
• Hamilton Method Editor - NIMBUS methods are written and edited in the
Method Editor.
• Hamilton Run Control - Methods are run via the Run Control. The Run
Control also provides a Control Panel for locking and unlocking the
NIMBUS door, among other functions.
2. Click Network and Internet > Network and Sharing Center >
“Change adapter settings.”
3. Select “Local Area Connection.” In the resulting Local Area
Connection Properties window, double-click the connection
“Internet Protocol Version 4 (TCP/IPv4).” Select “Use the
following IP address” and then type in the IP address
192.168.100.101.
4. Place your curser in the Subnet mask box. You should see
255.255.255.0 automatically appear (Figure 3-9).
5. Click OK to save the properties. Close any opened dialog boxes to
return to the desktop.
4. Click the Deck Configuration line on the right side of the window.
A browse button (a small box with three dots) will appear. Click the
box. The Deck Configuration window appears (Figure 3-11).
5. Select the radio button that matches the deck type of your NIMBUS
(9+2, 3x4, Shift-N-Scan). If you have a 9+2 deck, also select the
recess value from the drop-down list for the left side portion of your
deck (0, -12, -24, -36, -48 mm). Click OK.
The 9+2 NIMBUS deck comes from the factory with the lowered portion of the deck set to the lowest
position (-48 mm).
8. If you would like to enable Clot Detection with cLLD, then double-
click the “Clot Detection” line.
9. Leave the Labware Gripper option disable.
10. If you would like to enable Tip Type Detection, then double-click
on the “Hardware tip detection” line.
11. If you would like to enable Aspiration Monitoring with cLLD, then
double-click the “Monitor aspirate during cLLD” line.
12. The default for Daily and Weekly Maintenance is such that it is required
in order to operate the NIMBUS.
13. The programmer for the NIMBUS has the option of enabling the
various method programming steps. To do this, click on the “+”
symbol next to “Step Selection.” That opens up the list of options,
each of which can be set to Visible or Hidden.
14. If the TADM feature was installed, set the TADM mode to
Recording (if the method has not yet been defined) or to Monitoring
(if the method and its liquid class guard bands have been set).
15. Save the configuration and exit the Configuration Editor. Exit the
Run Control.
Chapter 4
Operation
Make sure there are enough tips to run the protocol. Make sure the tip waste
bin is empty. Make sure the tip eject plate is attached to the waste block.
Do not put anything on the main deck that sits higher than 118 mm above the deck. If using the
lowered portion of a 9+2 deck, avoid putting anything there that is taller than 166 mm.
To place a pedestal on the deck, align the holes on the left and right of the pedestal with the pins on
either side of the deck site.
If the NIMBUS is powered off, you can move the gantry by hand to give you full access to the deck. If
the NIMBUS is on and the door is closed, you can use the Control Panel in the Run Control to “Park”
the Gantry.
4.3 Running a
Method
To run a method:
1. Double-click on the Hamilton Run Control icon on the desktop.
2. From the Run Control window, click File > Open and browse to
find the method you wish to run.
3. Click on View on the menu bar. You can select which windows you
would like to see:
- Method View
- Nimbus Deck View
- Trace View
Once these windows are showing, you can resize and rearrange them how
you like. Figure 4-4 shows one way the windows can be arranged. The
views can also be turned on and off by clicking the appropriate toggle
buttons on the right side of the icon bar.
Figure 4-1shows the Run Control with a method ready to run. This shows the
Trace View (upper left), the Method View (right), and the Deck View (lower
left).
During a run, the deck view will be colorized, illustrating the activities that are taking place. The
method view will highlight the step that is being performed. The trace view will show text that
gives the details of the steps that were performed. The trace view details are also written to a log
file that is stored on the hard drive.
4. Methods can be run with simulation mode on or off. The Status box
in the Run Control will indicate if Simulation is on by having the
word “(Simulator)” showing. To toggle Simulator on or off, click
Settings > Simulation Mode.
5. Compare the deck view with what is on the deck of the NIMBUS,
and make sure they match. You can look at a close-up of the deck
view by clicking on it and using the mouse wheel to zoom in. You
can also use the various zoom tools found in the View menu. In
addition, the right edge of the deck view window has various icons
that match different views you can see: front, back, left, right, top.
6. When you are ready to start the method, close the door of the
enclosed NIMBUS. Then click the green Start button at the top of
the Run Control. You can also use the F5 button on the keyboard to
start a method.
7. To pause a run, either click the blue Pause icon at the top of the Run
Control (Figure 4-2). The NIMBUS will finish the last command it
received and then come to a stop. To resume after a pause, click the
icon again.
8. To abort a run, click the red Abort icon at the top of the Run Control.
A confirmation dialog will appear, asking if you are sure you would
like to abort the run. Click OK. The NIMBUS will finish the last
command it received, come to a stop, and the method will be aborted.
An aborted method cannot be restarted from the point it was aborted. An aborted method must restart
at the beginning of the method.
9. After a run, you can click the Control Panel icon at the top of the
Run Control to perform various actions: park the arm, eject the tips,
initialize the NIMBUS, and lock and unlock the door.
10. To run the method again, click the green Start button.
11. When you are finishing running the NIMBUS, close the Run
Control.
The recovery options are given as buttons at the bottom of the window. Here
are the options:
• Cancel. This will abort the method, unless the method has built-in error
recovery options.
• Abort. This will abort the method.
• Exclude. The channels that detected a missing tip will not be used in the
subsequent pipetting steps.
• Repeat. The channels will try again to pick up tips at that location.
• Next. The channels will move to the next location in the rack and attempt
to pick up tips from there.
The recovery options are given as buttons at the bottom of the window. Here
are the options:
• Cancel. This will abort the method, unless the method has built-in error
recovery options.
• Abort. This will abort the method.
• Bottom. The tips will go to the bottom of the container and aspirate from
there.
• Exclude. The channels that experienced the error will not be used in the
subsequent pipetting steps. This could cause errors in the subsequent
dispense step.
• Repeat. The channels will try again to find the liquid surface.
• Air. The tips will go to the clearance height and aspirate air.
Always have the NIMBUS door closed and locked before starting or resuming a run!
If you cannot reach the plate and the keyboard simultaneously, close the door,
click the “Lock Door” button, and then use the Move buttons to position the
plate onto a pedestal. When the plate is in a safe position to be released, click
the “Release Plate” button.
4. After releasing the plate, click “Park CORE Tool.” The CO-RE
paddles will be returned to their station. Close the dialog.
Clicking “Drop CORE Tool” will release the CO-RE paddles where the channels currently sit.
Chapter 5
Maintenance
If the NIMBUS is run more that about 12,000 tip eject cycles per channel per 6 months, then a more
frequent PM schedule is strongly recommended.
Dilute the concentrated Microcide SQ with deionized water according to the instructions on the bottle.
Do not use cleaning or disinfecting solutions which contain hypochlorite, such as bleach.
2. Move the gantry by hand to the far left of the deck. Remove the tip
waste bin from the NIMBUS deck. Remove the waste bin frame and the
tip waste bag. Dispose of the tip waste bag according to laboratory
procedures. Place a new bag in the bin and secure it with the bin frame.
Put the tip waste bin back in place on the deck of the NIMBUS. Refer
to Figure 3-5 thru Figure 3-7.
3. If the NIMBUS is equipped with the Liquid Waste System, inspect
the liquid waste carboy. Empty it if it is full or nearly full.
4. Click the checkbox marked “Empty Tip Waste / Liquid Waste.”
Doing so indicates you have performed this task.
5. Remove the tip eject plate from the waste bar to reveal the
maintenance needles. Clean the tip eject plate with soap and water
or with Microcide SQ. Dry thoroughly.
6. Close the door of the NIMBUS. Grab the handle, pull the door
toward you a short distance, and then move the door downward
until it is fully closed.
7. Click the checkbox marked “Check tightness of Pipetting Channels”
and click Continue. The instrument will initialize and then perform
an over- and then under-pressure check on each channel.
8. After the pipette channel pressure test has completed successfully,
click the checkbox marked “Check LLD” and click Continue. Each
channel will then perform a cLLD check by touching the corner of
the waste bar.
9. When the cLLD check is completed successfully, a box will appear
indicating the end of the Daily Maintenance (Figure 5-3). Click OK.
Close any open Maintenance window.
10. Open the door of the NIMBUS. Reinstall the cleaned tip eject plate.
Close the door.
6. Inspect the pedestals for damage as you place them back onto the
deck. Replace damaged pedestals.
7. Inspect any on-deck devices for damage. Replace or repair damaged
devices.
8. When finished, click the checkbox marked “Check condition of
carriers and other devices.”
9. Move the gantry by hand to the far left of the deck. Remove the tip
waste bin from the NIMBUS deck. Remove the waste bin frame and
the tip waste bag. Dispose of the tip waste bag according to
laboratory procedures.
10. Spray the waste bin and waste bin frame with Microcide SQ and
wipe with a paper towel.
11. Remove the tip eject plate from the waste bar. Clean the tip eject
plate with soap and water or with Microcide SQ. Dry thoroughly.
12. Spray Microcide SQ onto a paper towel and wipe down the tip
waste block and the area where the tip waste bin sits. Make sure the
waste block is dry.
Do not spray Microcide SQ onto the teaching needles. Do not wipe down the teaching needles. If
the teaching needles are dirty, remove them from the waste block and clean them with soap and
water. Rinse them with deionized water. Dry them thoroughly before returning them to the waste
block.
13. If the NIMBUS is equipped with the Liquid Waste System, inspect
the liquid waste carboy. Empty it if it is nearly full. Spray the
outside of the carboy with Microcide SQ and wipe down with a
paper towel. Wipe down the liquid waste tubing with a paper towel
wetted with Microcide SQ and the liquid waste tub on the waste
block.
14. Place a new bag in the tip waste bin and secure it with the waste bin
frame. Put the tip waste bin back in place on the deck of the
NIMBUS.
15. Click the checkbox marked “Empty and clean Tip Waste / Liquid
Waste.” Doing so indicates you have performed this task.
16. Gently pull each pipette head down from the Gantry to make them
accessible.
17. Spray deionized water onto a lint-free paper towel. See Figure 5-5.
For each channel, wipe down the tip eject sleeve (the black outer
part) of the pipette head with the wetted towel. See Figure 5-6.
18. Then lift up the tip eject sleeve and wipe the o-ring and stop disk. The
stop disk is the very bottom part of the pipette head. See Figure 5-7.
Do not get liquid inside the pipette head (inside the hole of the stop disk).
If it is necessary to move the channels in the Y direction, move them gently by hand by pushing close
to where they are attached to the Y spindle (the long threaded rod upon which the channels travel).
Never force them to move, as this may damage or misalign the channels.
19. When finished, click the checkbox marked “Clean each channel,
stop disk, o-ring, and tip eject sleeve.”
20. Spray the outer covers of the NIMBUS and wipe dry. Wipe the
inner covers and Gantry cover with a paper towel wetted with
Microcide SQ and wipe dry. When finished, click the checkbox
marked “Clean the side covers.”
21. Make sure the tip eject plate is not on the waste block. Close the
door.
22. Click the checkbox marked “Check tightness of Pipetting Channels”
and click Continue. The instrument will initialize and then perform
an over- and then under-pressure check on each channel.
23. After the pipette channel pressure test has completed successfully,
click the checkbox marked “Check LLD” and click Continue. Each
channel will then perform a cLLD check by touching the corner of
the waste bar.
24. When the cLLD check is completed successfully, a box will appear
indicating the end of the Weekly Maintenance (Figure 5-8). Click OK.
Close any open Maintenance window.
25. Open the door of the NIMBUS. Reinstall the cleaned tip eject plate.
Close the door.
5.6 Decontamination
To decontaminate the NIMBUS, perform the Weekly Maintenance. Use
Microcide SQ instead of water to clean the stop disks, o-rings, and tip
eject sleeves.
After Daily or Weekly Maintenance, ultraviolet germicidal
decontamination can be done (on systems with the UV option).
For systems equipped with the UV Lamp option, use the computer’s
desktop utility to activate the UV decontamination. The door of the
NIMBUS must be closed for the operation.
The utility will ask for the duration for UV decontamination. The UV
lamps will automatically shut off after the specified time (maximum of
one hour). During the decontamination, the Gantry will automatically
move form one side of the deck to the other, repeatedly. This is to
maximize the UV exposure to the surfaces inside the NIMBUS.
Decontamination by UV can only be done where the surface is directly
exposed to the UV light. Dirty surface, shadowed surfaces, or
unexposed surfaces are not decontaminated by UV exposure.
To replace a UV lamp:
1. Make sure lamp is cold and NIMBUS is powered off.
2. Open the NIMBUS door. Move the Gantry by hand to access the
lamp to be changed.
3. The lamp is secured by two sockets (UV Spring Socket and UV
Light Socket, see Figure 5-9). Push the lamp toward the spring
socket. This will free the other end away from its socket. Then pull
the lamp from the spring socket.
4. Dispose of the used lamp according to the local regulations.
5. Install the new lamp by putting one end into the spring socket and
then connect the other end into the fixed socket.
6. Verify the functioning of the new lamp. It is the responsibility of
your laboratory to verify the functioning of the UV system for your
particular needs.
Chapter 6
Parts and Accessories
6.2 Pedestals
61050-01 NTR pedestal, for nestable tip racks (NTRS) and for the small
tube adapter
61053-01 DWP pedestal, for deepwell plates and reservoirs, and for
PCR plate adapters
Part Number
6.7 Accessories
6.8 Consumables
Chapter 7
Technical Specifications
Parameter Specification
Throughput
4 x 1 mL: 540s to fill one 96-well microplate with 100 L
sample, with new tips for each
sample.
Operating
Temperature: 15o to 35oC (59o to 95oF)
Relative Humidity: 10% to 90% R.H. non-condensing
Storage
Temperature: -20oC to 70oC @ 90% humidity non-
condensing
CSA Certification
Installation category: II
Pollution degree: 2
Parameter Specification
Note: Indoor Operation and Use Only Per Section 3.6.6.2 “pollution degree 2” is
defined as “normally only non-conductive pollution occurs (addition of foreign
matter, solid, liquid, or gaseous (ionized gasses), that may produce a reduction of
dielectric strength or surface resistivity).” Occasionally, however a temporary
conductivity cause by condensation must be expected.
The design specifications mentioned above are valid under the following
No warranty can be given that the above specifications for trueness and precision are met with any
other liquid or environment than the ones specified.
7.2 Shift-N-Scan
7.2.1 Supported
Symbologies
The following bar code symbologies can be detected by the Shift-N-Scan
system:
• ISBT standard
• Code 128 (subset B and C)
• Code 39
• Codabar
• Code 2 of 5 Interleaved
• UPC A/E
• JAN/EAN 8
7.2.2 Reading
Accuracy
The rate of inaccurate reading of sample and container bar codes is less than 1
ppm.
The above-mentioned specification is valid under the following conditions:
• Bar code symbology module: ISBT standard
• Code density: 0.0065 in (0.1651 mm)
• Print contrast (PCS): > 80% ( = 650 nm)
• Bar code reading with error detected is defined as an accurate reading.
7.2.3 Barcode
Specifications
Code 39 None
Print quality The barcode print must be of a high quality. Offset, typographic,
intaglio and flexographic printing are suitable.
Mechanical dot matrix and thermo matrix printing are not suitable.
7.2.4 Positioning
Sample Barcodes
The label (Figure G-1) must be glued within a range of between 20 mm to 100
mm from the bottom of the tube.
The label must fit tightly at an angle of approximately 90° to the tube.
Parameter Specification
7.4 UV Lamp
The Supplied Uv lamps provide 50 W/cm2 of ultraviolet intensity at 254 nm.
Appendix A
Getting Technical Assistance
If you require additional assistance with your NIMBUS, please contact your
authorized Hamilton Company distributor or the Hamilton Company directly.
Please have the following information available when requesting technical
assistance:
• NIMBUS model number
• NIMBUS serial number
• NIMBUS configuration
• Description of the problem
• Your contact information
If possible, please also supply the following additional information:
• What was happening at the time of the error
• What you have tried so far to solve the problem
• Version of software (see “Finding the Software Version” section
on page A-2)
• Screen shots of any errors (see “How to Get a Screen Shot”
section on page A-2)
• Photos that could more readily illustrate the problem
• Trace files, log files (found in the” Hamilton Company / Logfiles”
directory)
• A copy of the method (see the NIMBUS IVD Programmer’s Manual,
65518-01, Rev. A.)
Please see below for contact information.
Appendix B
Deck Accessibility
The two dots shown in Figure B-2 at each corner of the deck represent the
extremes of travel for the two 5 ML channels. For example, the rear 5ml
channel cannot access the front three rows of wells in a 96-well plate in the
front row of pedestals.
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-----------
Appendix C
Regulatory
C.1 Regulatory
Affairs
CE, CSA and UL conformity are maintained for the NIMBUS.
C.1.2 In Vitro
Diagnostics
The MICROLAB® NIMBUS is an In Vitro Diagnostic Device and therefore
regulated by the Directive 98/79/EC of the European Parliament and of the
Council of 1998-10-27 on in vitro diagnostic medical devices.
C.1.3 Declaration of
Conformity
The declaration of conformity is part of the delivery of the NIMBUS
instrument.
Appendix D
Warranty
Glossary
Term Definition
ADC Anti Droplet Control to prevent drops while pipetting highly volatile solvents.
Barcode Mask The barcode mask defines the basic structure of a barcode. It is a pattern to which a
barcode must conform. The assignment of a specific Labware item can be done in
this manner. The barcode mask can require a barcode to contain specific strings at
fixed positions. It can also contain wildcards.
Container identification Barcode for the identification of a container. Serves for a unique identification of a
vessel, e.g. a sample test tube.
Deck The work surface (work area) of the NIMBUS. The area where the pipetting channels
perform liquid handling or transport steps.
Enclosure Protective covering for the NIMBUS Instrument, featuring a hinged front door made of
transparent polycarbonate. With this assembly, the work surface of the NIMBUS is
covered in such a way that it is shielded from user intervention and other outside
influences (such as dust). At the same time, it protects the user from the movements
of the NIMBUS.
Firmware Lower Level program code that is carried out on the processors of the NIMBUS
Instrument.
Hardware Error Type of error that is caused by a technical problem with the hardware.
HHS Hamilton Heater Shaker. Unit to heat of shake microplates in SBS format.
HSB Heater Shaker Box. Interface unit which is needed if more than two HHS are being
used.
Labware Refers to movable items to be placed on the NIMBUS deck, such as pedestals,
containers, or racks.
Glossary GL-1
MICROLAB® NIMBUS IVD Operator’s Manual (Rev. A)
Term Definition
LIMS Higher level data processing system, generally known as Laboratory Information
Management System, also LMS.
Liquid Includes all kinds of liquids, including reagents, controls, standards, and samples.
LLD (Liquid Level Detection of liquid surface which may be achieved either by pressure or capacitive
Detection) signal detection.
MAD (Monitored Air Aspiration monitoring feature. During the aspiration process, the pressure within the
Displacement) pipetting channel is measured in real time.
Method The method contains all instruction that must be executed during a run.
MTP (Microtiter plate) In general, a microtiter plate is assumed to have 96 wells (8 x 12) 9 mm apart.
There are also plates with 384 wells (16 x 24 / 4.5 mm), or others with a
different size.
Pedestal A block that sits on the deck of the NIMBUS and which holds plates, tips, reagents,
etc.
Pipetting arm Assembly equipped with the pipetting device and/or plate handler.
Pipetting channel Hardware that performs the functions of picking up a tip, aspirating, dispensing,
tip eject, liquid level detection and the Y/Z-movements.
Pooling Pipetting of different liquids in to one well.
Processing Step Defines what must be carried out on the NIMBUS instrument, as well as the location it
must be carried out and possible interaction with other system components or
labware. The action is defined in accordance with the methods, the loading and the
tasks.
Rack Group of containers, as DWP, MTP, etc.
Run Execution of the processing steps defined in the method with the aim of processing
one or more liquids and containers (e.g. MTP). The run is a series of timed
commands, in order to carry out processing on the NIMBUS according to the
processing plan.
Run abort Cancelled run by the user or by the NIMBUS.
Run visualization Visualization of the current run, reporting the status of the NIMBUS.
Side Touch Pipetting on the wall of a container of its center to prevent droplets.
TADM Total Aspiration and Dispense Monitoring. The pressure inside each individual
pipetting channel is monitored, during aspiration and dispensing.
Tip Disposable tip for pipetting.
GL-2 Glossary
MICROLAB® NIMBUS IVD Operator’s Manual (Rev. A)
Term Definition
Tube A container for liquid, usually having a circular cross-section and a cylindrical length
section.
User User of the software. Access rights for different types of users can be defined, such as
operators, laboratory manager, etc.
Waste Bin A device on the NIMBUS deck to collect used disposable tips.
Work area The area of the NIMBUS to which access is provided during the processing. Elements
to be pipetted or handled can be placed in this area.
Glossary GL-3
MICROLAB® NIMBUS IVD Operator’s Manual (Rev. A)
Index
A
About this manual 1-1
Accessing the Maintenance Utility 5-2
Accessories 2-25, 6-4
Hamilton Heater Shaker (HHS) 2-25
Liquid Waste System 2-26
Addendum
Hamilton Company instrument warranty D-1
Adjust Left Side of 9+2 Deck 3-2
After a Run 4-4
Air Displacement Pipetting 2-1
Anti-Droplet Control (ADC) 2-5
Aspiration Monitoring with cLLD 2-5
Axes and Dimensions 2-9
B
Barcode Specifications 7-4
Basic Instrument NIMBUS 7-1
C
Capacitive Clot Detection 2-5
Channels 2-10
Computer Requirements 1-7
Configuration Options 2-10
Channels 2-10
CO-RE Grippers 2-13
Decks 2-10
Ultraviolet (UV) Lamp 2-13
Connect Cables 3-1
Consumables 6-4
CO-RE Gripper 7-6
CO-RE Grippers 2-13, 6-3
D
Deck Accessibility B-1
Decks 2-10
Decontamination 5-9
Description of the NIMBUS 2-1
Disposable CO-RE Tips 2-20
Nestable Tip Racks (NTRs) 2-21
Tips in Frames 2-22
Disposable Tip Attachment with the CO-RE Technology 2-2
Disposable Tips 6-1
Door Not Locked 4-8
F
Features of the Base Instrument 2-7
Finding the Software Version A-2
IN-1
MICROLAB® NIMBUS IVD Operator’s Manual (Rev. A)
G
General Information 1-1
About this manual 1-1
Getting Technical Assistance
Finding the Software Version A-2
How to Get a Screen Shot A-2
Technical Support in Europe, Asia, and Africa A-2
Technical Support in the Americas and Pacific Rim A-1
Glossary GL-1
Gripped 4-11
H
Hamilton Company instrument warranty D-1
Hamilton Heater Shaker (HHS) 2-25, 6-3
Handling Errors during a Run 4-5
Door Not Locked 4-8
Liquid Not Found 4-7
Missing Tips 4-5
Object Gripped 4-11
Power Off 4-10
How to Get a Screen Shot A-2
I
Important symbol 1-1
Install NIMBUS Software 3-5
Install the Waste Bin 3-4
Intervals 5-1
L
Liquid Level Detection (LLD) 2-3
Liquid Not Found 4-7
Liquid Waste System 2-26
M
Maintenance 5-1
Maintenance Fluids 6-4
Materials Required 5-1
Missing Tips 4-5
Monitored Air Displacement (MAD) 2-4
N
Nestable Tip Racks (NTRs) 2-21
Note symbol 1-1
O
Operation 4-1
P
Parts and Accessories 6-1
Accessories 6-4
Consumables 6-4
CO-RE Grippers 6-3
Disposable Tips 6-1
Hamilton Heater Shaker (HHS) 6-3
Maintenance Fluids 6-4
Pedestals 6-2
IN-2
MICROLAB® NIMBUS IVD Operator’s Manual (Rev. A)
R
Reading Accuracy 7-3
Replacing UV Lamp 5-10
Running a Method 4-2
S
Safety Symbols 1-1
Set the Computer's IP Address 3-6
Set the NIMBUS Configuration 3-7
Setting up the Deck 4-1
Setting up the NIMBUS 3-1
Shift-N-Scan 7-3
Barcode Specifications 7-4
Positioning Sample Barcodes 7-5
Reading Accuracy 7-3
Supported Symbologies 7-3
Software and Manuals 6-4
Supported Symbologies 7-3
Symbols
Important 1-1
Note 1-1
Procedure 1-2
Warning 1-1
IN-3
MICROLAB® NIMBUS IVD Operator’s Manual (Rev. A)
T
Teaching Needles 6-3
Technical Support in Europe, Asia, and Africa A-2
Technical Support in the Americas and Pacific Rim A-1
Tip Size Recognition 2-3
Tip Waste 2-23, 6-3
Tip-On Recognition 2-3
Tips in Frames 2-22
Total Aspiration and Dispense Monitoring (TADM) 2-6
Turn on the NIMBUS 3-10
U
Ultraviolet (UV) Lamp 2-13
UV Lamp 7-6
W
Warning symbol 1-1
Waste Electrical and Electronic Equipment (WEEE) 1-6
IN-4