2CAZO NY Taventews Operator’s Reference Manual MODEL 340 SPECTROPHOTOMETER SEQUOIA-TURNER CORPORATION 850 Maude Avenue, Mountain View, CA 94043 (415) 969-5533, Telex 4947667 Printed: October 1986 PIN: 0340001PRODUCT WARRANTY Your SEQUOIA-TURNER Model 340 Spectrophotometer is guaranteed to be free of defects in workmanship and materials under normal use for a period of one year from the date of purchase to the consumer. ‘The Hebility of SEQUOIA-TURNER CORPORATION is limited to repair or replacement and in no event shall SEQUOIA-TURNER be liable for any collateral or consequential damages or loss. Instruments subjected to misuse, abuse, neglect or unauthorized repair or modifications will be excluded from this warranty. This guarantee specifically excludes expendables and consumables, All warranty claims must be directed to the SEQUOIA~TURNER CORPORATION distributor responsible for the sale of the instrument.SECTION 1 1 1-2 SECTION 2 21 2-2 2-3 SECTION 3 3 3 3 SECTION 4 a1 a2 4-3 4-4 a5 46 SECTION 5 SL 5-2 TABLE OF CONTENTS GENERAL SYSTEM INTRODUCTION System Description Specifications INSTALLATION Standard Equipment Instrument Checkout Installation OPERATION Deseription Operation Analog Output PREVENTIVE MAINTENANCE General Fuse Replacement Cuvette Holder Removal Lamp Replacement Cover Removal Voltage Selection TROUBLESHOOTING General TroubleshootingSEQUOIA-TURNER MODEL 340 SECTION 1 GENERAL SYSTEM INTRODUCTION 1-1 SYSTEM DESCRIPTION ‘The monochromator on the Model 340 Spectrophotometer consists of a plane diffraction grating in an {7 Ebert mounting with first surface reflective optics. This design ensures efficiency and gives the 340 a continuous wavelength range of 330nm to 1000nm to cover most routine ULV. and colorimetric tests. This range can be expanded to 195nm with a Far U.V. accessory. Only three operator controls make the 340 one of the simplest spectrophotometers in the world to use. A large 3 1/2 digit LED display provides easy readability to minimize errors. With a simple turn of a knob, the readout can be switched from percent transmittance to absorbance. It's so easy, the Model 340 requires virtually no training time. 1-2 SPECIFICATONS Readout ~ Seven segment, 3 1/2 digits, selectable in two mod 0.0-100.0 percent Transmittance 071.999 Absorbance Wavelength Range - 330-1000nm (extendable to 195nm) Monochromator - Diffraction grating, f7 Ebert Spectral Bandwidth - 8.0nm nominal Wavelength Accuracy - within 2nm over full range Wavelength repeatability - within 0.5nm. Stray Radiant Energy - less than 0.5% Photometric Linearity - better than 1% Detector ~ Solid state silicon device Standard sample holder - accommodates 13 x 100 mm round cuvettes (adapters for other round and square cuvettes available) Outputs - 0-1.0VDC linear in transmittance Power requirements - 100, 120, 220, 240 VAC, 50/60 Hz, 50 watts Dimensions width-39em (15 3/8") X depth-29 em (11 1/2") X height-13 em (4 1/2") Shipping Weight - 7.3Kg (16 Ib.) LlSEQUOIA-TURNER MODEL 340 SECTION 2 INSTALLATION 241 Standard Equipment ‘The SEQUOIA-TURNER MODEL 340 consists of the following: Model 340-000 Digital Spectrophotometer Power Cord 330-199 Cuvette Holder for 13 mm round cuvettes (installed) 380-005 Stray Light Filter (UV) for 330-385 nm 380-006 Stray Light Filter (VIS) for 385-500 nm 380-007 Stray Light Filter” (VIS) for 420-890 nm (installed) 380-008 Stray Light Filter (IR) for 725-1000 nm 1/16" Allen Wrench 0.6 Amp Slow Blow Fuse (for 100 and 120 VAC use only) 0.3 Amp Slow Blow Fuse (for 220 and 240 VAC use only) 340-001 Operation Manual Dust Cover 2-2 Instrument Checkout After the 340 is unpacked inspect it for physical damage. Report any observed damage immediately to the Freight Carrier and your dealer. 2-3 Installation ‘A. The 340 requires either 100, 120, 220 or 240 VAC at 50 Hz or 60 Hz from a properly wired and grounded, single phase power outlet. The 340 is delivered preset to 120 VAC. Verify which voltage is present and set the voltage selection board on the back panel (see FIG 2-1) to the appropriate setting as follows: 1. Locate power inlet connector on back panel. 2, Be sure the power cord is not installed and slide the plastic protective cover to the left exposing the voltage selector board. If the voltage shown is incorrect, pull back the black fuse lever to remove the fuse and remove the board by pulling it firmly with a set of small pliers. 3, Turn the board until the proper voltage is facing you and reinsert the board. The proper voltage is now visible from the rear of the instrument. Insert the proper fuse for the voltage selected. For 100 and 120 VAC use 0.6 Amp Slow Blow fuse. (installed) For 220 and 240 VAC use 0.3 Amp Slow Blow fuse. (included in accessories) 21SEQUOIA-TURNER MODEL 340 CAUTION ‘Two spare fuses are provided with the instrument, 0.3 Amp and 0.6 Amp. In addition, the instrument comes with a 0.6 Amp fuse installed for 100 and 120 VAC operation, In areas where 220 and 240 VAC are used, the installed fuse and the spare 0.6 Amp fuse should be discarded and replaced with the 0.3 Amp fuse. 5. Slide the cover to the right. B, Locate the instrument on a vibration-free laboratory surface. Allow two inches behind panel for air circulaton and power cord. C. Connect the power cord to instrument and to the proper outlet after verifying that the voltage of the instrument has been properly set. D, Turn power switch (left hand knob on front panel) to Transmission Mode. The instrument panel should illuminate, If the display does not illuminate, immediately turn the instrument off and consult the troubleshooting section (see section 5-2). E. Adjust lamp for maximum output (see section 4-4). F, Refer to section 3 for operational details of the 340. Analog Out Fuse Puller Fuse NX Voltage a Selector Lamp Cover Power Connector FIG 2-1, Back Panel, Model 340 2.2SEQUOIA-TURNER MODEL 340 SECTION 3 OPERATION 3-1 Deseription Refer to FIG 3-1 for location of the components. A. Controls and Indicators: 1. MODE SWITCH - OFF - Turns instrument off. TRANS - Turns power on and places instrument in transmission mode. ABS - Turns power on and places instrument in absorbance mode. 2. ZERO - When instrument is in TRANS mode and the ZERO SET button is pressed and held, this’ knob is used to set the display to 0.0 % T. 3. 100%T/0A - In TRANS mode is used to set 100% T on the display. In ABS mode is used to set .000 A on display. Consists of two knobs, a larger knob next to the panel for coarse setting and a smaller knob in front for fine setting. 4, DISPLAY - 3 1/2 digit display to indicate transmission or absorbance. 5. TRANS - Indicator LED lights when the instrument is in transmission mode. 6. ABS -Indicator LED lights when the instrument is in absorbance mode. 7, WAVELENGTH READOUT - 3 digit readout of the wavelength in nanometers, 8. WAVELENGTH ADJUST - Thumbwheel knob to set the wavelength of the instrument. 9, ZERO SET - Button is pressed when the instrument is adjusted to 0.0% T with the ZERO knob. - B, Cuvette Holder: The standard cuvette holder supplied with the instrument takes a 13mm by 100mm tube, To insert tube, lift cover from the front and insert tube until it reaches the bottom. Close cover. See your dealer for optional cuvette holders available for your 340. C. Stray Light Filters: For optimum performance, a set of stray light filters are supplied, covering the wavelength range of 330 to 1000nm. To change filters, push back handle on filter slightly to disengage lock and lift straight up. Insert proper filter (it will automatically lock into place). The instrument must have a stray light filter in position for proper operation. 3.1SEQUOIA-TURNER MODEL 340 4, DISPLAY 5. TRANS 6. ABS at 8. WAVELENGTH ADJUST LIGHT FILTER ., CUVETTE HOLDER 1. MODE SWITCH 2. ZERO 3. 100%T/0A 7. WAVELENGTH 9. ZERO COARSE FINE READOUT SET FIG 3-1 SEQUOIA-TURNER Model 340 Spectrophotometer 3-2 Operation Perform proper installation steps (see section 2) before instrument is operated for the first time. A. Turn mode switch to TRANS, Allow a 15 minute warm-up. B. Adjust instrument to desired wavelength. C. Insert appropriate stray light filter into position. D. Place cuvette with water or other blank solution into cuvette holder. E. Press and hold ZERO SET button while adjusting ZERO knob until display indicates 0.0. Release ZERO SET button.SEQUOIA-TURNER MODEL 340 1, For transmission measurements: a. Set MODE switch to TRANS. b. Adjust 100%T/0A COARSE knob to approximately 100. ¢. Adjust 100%T/0A FINE knob to exactly 100.0. d. Replace cuvette with sample cuvette and read transmission from digital display. 2. For absorbance measurements: a, Set MODE switch to ABS. b. Adjust 100%T/0A COARSE knob to approximately .000. ¢. Adjust 100%T/0A FINE knob to exactly .000. d. Replace cuvette with sample cuvette and read absorbance from digital display. 33 Analog Output External devices, such as data processing instruments or strip chart recorders, may be interfaced to the 340, Spectrophotometric analog output is on the rear panel (see FIG 2-1). The zero to one volt DC output is equivalent to the transmittance of the sample whether the 340 is in ABS or TRANS mode. Connection is made to the 340 with standard banana plugs. Observe the polarity as marked on the panel. 3.3SEQUOIA-TURNER MODEL 340 SECTION 4 PREVENTIVE MAINTENANCE 4-1 General ‘The 340 has been designed with the same reliability inherent in previous TURNER spectro- photometers but with modern solid state electronics. It requires minimal preventive mainten- ‘ance and only the care one would give any quality instrument. It should be kept clean and out of dusty areas or those with temperature and humidity extremes, 4-2 Fuse Replacement : ‘The fuse is mounted in a holder which prevents access unless the power cable is removed. First turn off instrument and then remove cable from power outlet. ‘A. Remove power cable from back of instrument. B, Slide plastic cover to left exposing fuse holder. C. Locate black lever marked FUSE PULL. Swing lever out and to the left. D. The fuse can now be removed. E. Replace the fuse only with the proper Slow-Blow type. If voltage is changed refer to installation instructions (section 2-3) . 0.6 Amp for 100 and 120 VAC applications 0.3 Amp for 220 and 240 VAC applications F, Insert fuse and push lever back into place. G. Slide cover to right and replace power cord in instrument and then into power outlet. 4-3 Cuvette Holder Removal SEQUOIA-TURNER manufactures a variety of cuvette holders to increase the versatility of the 340. The holders are easily removed as follows: A, Lift the cover on the cuvette holder. Locate the Allen screw at the inside back of the cuvette holder and about 1 em (.4") down. B, Insert the 1.6mm (1/16") Allen Wrench into the screw and loosen it several turns (CCW). C. Lift the cuvette holder out of the instrument. 41SEQUOIA-TURNER MODEL 340 D, Reverse the steps above to install the alternate cuvette holder. 4-4 Lamp Replacement A. Turn the instrument off. B. Locate the two thumb screws on the lamp housing on the right side of the instrument. C, Remove the two thumb screws and remove the lamp housing cover. D. The lamp is removed by pressing it toward the rear of the instrument while turning it counter-clockwise. Install a new lamp (Sequoia-Turner part number 330-058). Clean the lamp with lens tissue. F, Align the lamp. 1, Turn the instrument on, 2, Set the instrument in TRANS mode and adjust the wavelength to 340nm with the UV (330-385nm) stray light filter in place. 3. Adjust the 100%T/0A knob for about 50% Transmittance on the display. If it does not affect the readout set it to maximum, 4. Grasp the black knob at the base of the lamp holder and move it until the display increases. Continue adjusting until the display is at a maximum, The 100%T/0A knob may have to be turned down to keep the display from overranging. The filament should be kept in vertical alignment at all times. 8. When the reading is maximized, turn the instrument off and replace the lamp housing cover. 4-5 Cover Removal NOTE This should only be performed under the direction of a qualified service representative. There are no user adjustable or replaceable parts inside this instrument. ‘A. Turn off instrument and remove the power cable. B, Remove the cuvette holder (see section 4-3). C, Turn the instrument over and remove the six screws holding the cover on. D. Turn the instrument right side up and lift the cover off. E, Reverse the above steps to replace cover. 4-6 Voltage Selection See section 2-3 in the installation procedure. 42SEQUOIA-TURNER MODEL 340 SECTION 5 ‘TROUBLESHOOTING 5-1 General ‘The 340 has no user replaceable or adjustable components inside the cover. Do not remove the cover unless specifically authorized by the dealer or the factory. If steps below do not solve problem contact your dealer's service representative. 5-2 Troubleshooting * Front panel does not illuminate - 1. Turn power off. 2, Remove power cord from instrument and main power line. 3. Remove and inspect fuse (see section 4-2). Replace if necessary. 4. Reinsert power cord firmly to ensure proper contact. 5. Turn instrument on and verify proper operation. * Front panel illuminates, source lamp does not - 1. Turn coarse 100%T/0A knob fully clockwise. 2. If lamp still does not illuminate, replace and align lamp (see section 4-4). 3. Turn instrument on and verify proper operation. * Front panel and source illuminate, display will not change - 1. Perform installation procedure (see section 2-3) and operation procedure (see section 3-2). 5.1