The Process in Which A Comminuted Drug or Sample Plant Is Extracted Its Soluble Constituents by The Slow Passage of Suitable Solvent Through A Column of Drug After Imbibition
Test performed Expected positive Result with Interpretation of result with
result sample discussion 1. Molisch test purple or red positive Molisch’s test is a chemical test which is used to check for the presence of carbohydrates in a given analyte. Since sugar is a carbohydrate it will definitely have a positive result. We had a red color on this test. 2. Fehlings test Yellow to brick red positive a clear blue solution containing copper sulfate and a colorless solution containing sodium tartrate. You add both solutions to the sugar and place the entire mixture in boiling water. If the sugar is reducing, a brick red precipitate forms. If you add sucrose or another non- reducing sugar, the mixture stays clear blue. 3. Barfoeds test Red precipitate Negative The negative result indicates that it has no reducing monosaccharide. 4. Osazone test Crystals positive Needle shape glucosazone crystals as viewed under the microscope. 5. Seliwanoffs Red color is produced positive This test is given positive by test ketohexoses so it is answered by fructose, sucrose and other fructose containing carbohydrates. Ketohexose containing disaccharide. Upon continuous boiling, aldoses get converted to ketoses and give a positive reaction with Seliwanoff reagent. 6. Aniline Red color is produced positive The paper turns white to slight acetate test red. Presence of pentose sugar in the supplied sample. 7. Mucic acid Crystals positive The dicarboxylic acid formed test from galactose is called mucic acid and is insoluble in cold aqueous solution. Those acids formed from the other common sugars are soluble in H20. Thus the formation of the insoluble precipitate is an indication of the presence of galactose. 8. Iodine test Blue color is formed positive The solution turns blue immediately due to the formation of starch-iodine complex, or the solution turns reddish-brown.
Test performed Expected positive Result with sample Interpretation of
result result 1. Molisch test purple or red Negative 2. Fehlings test Yellow to brick red Negative 3. Barfoeds test Red precipitate Negative 4. Osazone test Crystals Negative 5. Seliwanoffs Red color is produced Negative test 6. Aniline Red color is produced Negative acetate test 7. Mucic acid Crystals Negative test 8. Iodine test Blue color is formed Negative
The Process in Which A Comminuted Drug or Sample Plant Is Extracted Its Soluble Constituents by The Slow Passage of Suitable Solvent Through A Column of Drug After Imbibition