Ćelijske kulture

FBS

penicilin 100 UI/ml ?

streptomycin 100 µg/ml

DMEM 10 ml

Ekstrakcija

Gingko Biloba leaves 150 g

10% methanol (liquid) 10 ml

70% ethanol 5 ml

ethyl acetate 30 ml

sodium sulfate

acetone 3 ml

Bioautografija

ethyl acetate

acetic acid

formic acid

agar

MTT test

4 mM HCl

0.1% NP40

MTT 3-(4 5-dimethylthiazol-2-yl)-2 5-diphenyltetrazolium br…) 10 µl

Phosphate-buffered saline (PBS) 5 ml

Trypan Blue

0.4% Trypan Blue solution 3 ml

Comet assay

agarose (solid) 3g

PBS 3g

LMA 3g

NaCl (solid) 50 g

Tris 3g

NaOH 40 g

Triton X-100 5 ml

Na2EDTA 3g

DMSO 20 ml

ethidium bromide 0.1 ml

Ćelije
6
HeLa cells 2 X 10
6
fibroblast cells 2 X 10