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LWT - Food Science and Technology 125 (2020) 109216

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LWT - Food Science and Technology


journal homepage: www.elsevier.com/locate/lwt

Intelligent evaluation of black tea fermentation degree by FT-NIR and T


computer vision based on data fusion strategy
Ge Jin, Yujie Wang, Luqing Li, Shanshan Shen, Wei-Wei Deng, Zhengzhu Zhang, Jingming Ning∗
State Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Science & Technology, Anhui Agricultural University, Hefei, Anhui, 230036, PR China

A R T I C LE I N FO A B S T R A C T

Keywords: Fermentation is the most crucial step in black tea processing. In this study, we used a combination of Fourier
Black tea fermentation time transform near-infrared spectroscopy (FT-NIR) and computer vision system (CVS) for comprehensively evalu-
Computer vision ating the black tea fermentation degree. 110 samples of black tea at different fermentation degrees were col-
FT-NIR lected in this research. The tea polyphenols (TP) content was measured using a UV–Vis spectrometer, and TP
Mid-level fusion
degradation during black tea fermentation was measured for classifying the degree of fermentation. Two mid-
Tea polyphenols
level strategies were used to analyze the fusion signals of FT-NIR and CVS. K-nearest neighbors, linear dis-
criminant analysis, and a support vector machine (SVM) were applied for classification modeling. The ad-
vantages of FT-NIR and CVS were integrated based on mid-level fusion, and the results obtained were better than
those obtained using independent methods for evaluating black tea fermentation. The mid-level fusion strategy
that combined FT-NIR and CVS for principal component analysis feature extraction was the most effective in the
SVM model. Classification accuracies of calibration and prediction sets were each 100%. Therefore, in this study,
it was demonstrated that a combination of FT-NIR and CVS at mid-level fusion strategy can be used as a method
to rapidly evaluate the black tea fermentation degree.

1. Introduction changes in the color and smell of tea leaves (Zhu et al., 2019); however,
experience, psychological state, environment, and other factors affect
Black tea is one of the most enjoyable and popular beverages in the the evaluation process, making it highly subjective. To objectively
world (Gallardo, Alegret, & Valle, 2005; Mao et al., 2018) and accounts evaluate black tea fermentation, some nondestructive and rapid de-
for 78% of tea consumption worldwide (Kraujalytė, Pelvan, & tection techniques have been applied. An electronic nose device
Alasalvar, 2016). Black tea is made from fresh tea leaves in five steps (Bhattacharyya et al., 2007; Sharma et al., 2015) and an electronic
(Lin & Zhu, 2004), namely withering, rolling, fermentation, drying, and tongue (Ghosh et al., 2015) were developed to evaluate black tea fer-
sorting. Fermentation is considered the most critical step in black tea mentation. However, the aforementioned methods are complex, greatly
processing. The essence of fermentation is the oxidation reaction of tea affected by the environment, and costly. Therefore, evaluating black tea
polyphenols (TP). As fermentation duration increases, TP content de- fermentation in a simple, quick, and accurate manner remains a pro-
creases. At this point, a series of chemical reactions occur, including the blem to be solved urgently.
formation of theaflavins (TFs) and thearubigins (TRs) from catechins Near-infrared spectroscopy (NIR) is advantageous because it allows
under the action of polyphenol oxidase (PPO) and peroxidase (PO) rapid and nondestructive evaluation of tea leaf quality (Chen, Zhao,
(Marie-Claude, Shengmin, Yang, Chi-Tang, & Rosen, 2004; Samanta Huang, Zhang, & Liu, 2006; Li, Jin, Sun, Ye, & Liu, 2019). Favorable
et al., 2015). Lipids, amino acids, carotenoids, and terpenoids form results were achieved when NIR spectroscopy was used to monitor the
some volatile organic compounds. These compounds together impart ratio of TFs, TRs (Dong, Li, et al., 2018), and phenolic compounds
black tea its distinctive flavor (Gill, Kumar, & Agarwal, 2011). How- (Zareef et al., 2019) in black tea fermentation. However, it only mon-
ever, insufficient or excessive fermentation affect black tea quality itored chemical changes during fermentation and overlooked color-re-
(Jolvis Pou, 2016). Therefore, identifying the most appropriate amount lated information. The black tea fermentation evaluation method is
of black tea fermentation is essential. Exploring TP degradation is relatively single-faceted. A previous study used computer vision system
conducive to achieve the most appropriate fermentation degree. (CVS) to evaluate black tea fermentation (Dong, Liang, et al., 2018);
Traditionally, artisans evaluate the fermentation degree based on however, the study only analyzed the correlation between color


Corresponding author.
E-mail address: ningjm1998009@163.com (J. Ning).

https://doi.org/10.1016/j.lwt.2020.109216
Received 17 November 2019; Received in revised form 17 February 2020; Accepted 27 February 2020
Available online 03 March 2020
0023-6438/ © 2020 Elsevier Ltd. All rights reserved.
G. Jin, et al. LWT - Food Science and Technology 125 (2020) 109216

characteristics and fermentation quality indexes. Further research is and divided into calibration set and prediction set according to a ratio
required to comprehensively evaluate the relationship among fermen- of 2:1. The calibration set was used for calibrating model and the
tation color, chemical composition change, and black tea fermentation prediction set that used to test the robustness of the model. Eventually,
degree. A single sensor is insufficient for evaluating the high complexity the calibration set contained 73 samples and the prediction set con-
of food, and data fusion can improve food quality evaluation (Rosa, tained 37 samples. To examine the performance of the model, correct
Leone, Cheli, & Chiofalo, 2017). Using NIR spectroscopy and CVS to classification rate (CCR) were used:
evaluate fish freshness (Huang et al., 2016; Wu, Yang, Zhou, Lai, &
NRight
Zhong, 2019) is more effective than using a single sensor. CCR =
N
This study established a quick, simple, and objective method for
determining the black tea fermentation degree; we combined Fourier where NRight is the number of correct classified samples in calibration
transform near-infrared spectroscopy (FT-NIR) and CVS. No study has set or prediction set, N is the total number of samples in calibration set
reported the application of data fusion technology to evaluate black tea or prediction set.
fermentation. The objectives of this study were (1) to investigate TP Considering color and spectral characteristics as independent vari-
content at different fermentation time; (2) to compare the performances ables and degradation results of four groups of TP as dependent vari-
of FT-NIR and CVS in evaluating black tea fermentation; and (3) to ables, a fermentation degree discrimination model was developed.
compare and analyze the feasibility and advantages of principal com-
ponent analysis (PCA) and successive projections algorithm 2.2. Image acquisition
(SPA) + Pearson correlation analysis (Pearson) feature extraction data
fusion methods. The CVS, comprising a lighting room and digital camera, was used
to obtain image information during black tea fermentation. To ensure
2. Materials and methods light source stability, a 40 × 40 × 40 cm3 black box was equipped with
two fluorescent lamps. The light was turned on 30 min before image
2.1. Sample preparation collection. In addition, a digital camera (ESO 700D, Canon) with a re-
solution of 2592 × 1728 pixels was installed, with the distance be-
Black tea samples at different fermentation time were collected from tween the camera and the sample fixed at 40 cm. Images were acquired
the Jindong tea factory (Huangshan, Anhui) on April 19, 2019. The using the following camera settings: manual exposure with a shutter
samples were produced using traditional manufacturing processes, such speed of 1/100 s (zoom and flash functions off) and an ISO number of
as tea leaf collection (one bud with two leaves), withering, rolling (with 400. All the images were captured using the same camera settings. A
a rolling machine diameter = 90 mm), and fermentation at different previous method (Huang et al., 2016) was used as reference to extract
time intervals. The finely crushed tea leaves were placed in a fermen- color features (R, G, B, H, V, S, L*, a*, and b*) from the images.
tation room where the temperature was controlled at 30 ± 3 °C and
humidity was 90 ± 5%. The fermentation time was 300 min, and 10 2.3. Spectra acquisition
samples were collected every 30 min, that is, a total of 110 samples
were collected. During sample acquisition, computer vision experi- The black tea fermentation samples were removed from the −80 °C
ments were performed. The 110 collected samples were placed in a refrigerator. Then recover to room temperature in the drying vessel.
storage box with dry ice during transportation to the laboratory. The samples were scanned in a diffuse reflection mode using an FT-NIR
Finally, they were stored in a refrigerator at −80 °C until further ex- spectrometer (Bruker Optics Inc, Ettlingen, Germany). Each spectrum
perimentation. was the average of 32 scans recorded between 800 and 2500 nm, with a
The experimental process is presented in Fig. 1. In addition, before resolution of 8 cm−1. The samples were analyzed in triplicate with a
modeling, The 110 fermented samples were numbered consecutively rotation of 120° after each step. Spectra were collected at an ambient

Fig. 1. Flowchart of the primary step in data fusion analysis for evaluating black tea fermentation.
TP: Tea polyphenols; PCA: Principal component analysis; SPA: Successive projections algorithm; Pearson: Pearson correlation analysis.

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G. Jin, et al. LWT - Food Science and Technology 125 (2020) 109216

Fig. 2. Scheme of data fusion approaches used to combine the CVS and FT-NIR.

temperature of 25 ± 3 °C (Li, Wei, Ning, & Zhang, 2015). (Gallardo et al., 2005).

2.4. Extraction and determination of TP 2.5.2. K-nearest neighbors


K-nearest neighbors (KNN) is a classification method based on the
TP content was determined using a UV–Vis spectrophotometer assay close proximity of similar samples in a pattern space. It calculates the
according to the Chinese standard method (GB/T8313-2018). In a 10- distance between K known samples in the nearest field and the position
mL centrifuge tube, 0.2 g of the black tea fermentation sample and of the samples to be judged (Berrueta, Alonso-Salces, & Héberger,
5.0 mL of 70% methanol were mixed. The mixture was extracted at 2007). The optimization analysis of parameter k is necessary to improve
70 °C for 10 min, cooled to room temperature, and then centrifuged at the performance of the KNN classifier (Chen, Zhao, Chen, Lin, & Zhao,
3500 rpm for 10 min. The supernatant was transferred to a 10-mL 2011). If k is too large, the model becomes simpler and big classes will
volumetric flask. The aforementioned steps were repeated to re-extract overwhelm small ones; if k is too small, the model becomes complex
the supernatant in the next step. The supernatants obtained in each step and prone to overfitting. According to the principle of cross validation
were mixed in a 10-mL centrifuge tube, and deionized water was added of highest classification accuracy, k value was screened and optimized
to obtain a 10 mL solution. Then, 1 mL TP extract was added to a 100- to obtain the best model results.
mL volumetric flask, and deionized water was added to obtain a 10 mL
solution. 2.5.3. Linear discriminant analysis
Gallic acid solution (10, 20, 30, 40, and 50 μg/mL), water (1 mL), LDA was one of the most popular supervised methods, which at-
and polyphenol extract (1 mL) were added to a 10-mL volumetric flask, tempts to find linear combinations of features to find the best linear fit
and Folin Ciocalteu was added (5 mL, 10.0%). After a 5-min reaction, for separating two or more sets of samples (Chen, Ke, Cai, Zhu, & Chen,
4 mL of 7.5% Na2CO3 was added and allowed to remain for 1 h at room 2015).
temperature. A UV–Vis spectrophotometer was used to determine the
absorption value of the test solution at a wavelength of 765 mm.
2.5.4. Support vector machine
The main idea of the support vector machine (SVM) is to establish a
2.5. Multivariate data analysis classification hyperplane as the decision surface. It is advantageous
because of its nonlinear and small sample sets (Min & Lee, 2005). In this
2.5.1. Principal component analysis study, SVM was built using RBF as kernel function. RBF kernel function
PCA is the most commonly used dimension reduction technology parameters, namely c and gamma (g), are crucial for designing effective
that converts original variables into new irrelevant variables, which is SVM models. The parameter c minimizes the fitting error and the
called principal components (Bro & Smilde, 2014). The purpose is to complexity of the model, while g defines the nonlinear mapping from
improve the data processing speed and saves considerable time and cost the input space to a high-dimensional feature space. In order to obtain

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G. Jin, et al. LWT - Food Science and Technology 125 (2020) 109216

the best generalization performance, the grid-search program searches black tea fermentation was moderate. After 3.5 h, the TP continued to
for the optimal combination of (c, g) through 5-fold cross validation degrade, and the lowest degradation rate of TP was 28.63%, which
and the ranges of c and g were both 2−10-210 (Li, Xie, Ning, Chen, & resulted in excessive fermentation. The decline trend of TP content was
Zhang, 2019). similar to the results of the study (Muthumani & Kumar, 2007).
Therefore, the black tea fermentation samples could be classified ac-
2.5.5. Data fusion strategy cording to the TP degradation rate as preliminary, insufficient, mod-
The data can be combined basically at three levels, namely low-, erate, and excessive fermentation.
mid-, and high-level fusion. In mid-level fusion, informative features of
the raw data from each instrument are separately extracted using sev- 3.2. Computer vision data analysis
eral types of variable selections or feature extraction algorithms and are
then aligned and concatenated into a single matrix that is used for With an increase in the fermentation duration, the color of black tea
multivariate analysis (Borràs et al., 2015). Fig. 2 summarizes that in changed to some extent. During fermentation, PPO oxidized TP to form
this study, two mid-level fusion methods combine two different feature o-quinones (a highly unstable form), which then formed TFs and TRs. In
extraction information blocks. The mid-level fusion of PCA feature ex- addition, chlorophyll degraded to form pheophytin (Gill et al., 2011).
traction consisted of merging variables that had been previously se- During fermentation, these compounds led to the change in the black
lected by applying the PCA dimension reduction technology. Char- tea color. Black tea images at different fermentation time are presented
acteristic variables with cumulative variance > 99% were extracted. in Fig. 3. During black tea fermentation, the color changed from green
FT-NIR and CVS both generated three new variables, respectively. For to deep copper red. To accurately classify black tea fermentation ac-
the mid-level fusion of SPA and Pearson feature extraction, three cording to color characteristics, multivariate analysis techniques were
variables extracted using FT-NIR were selected for SPA, and four vari- used, which include different algorithm types. Three classical modeling
ables extracted using Pearson correlation (> 0.6) were selected for CVS methods, namely KNN, LDA, and SVM, were used to model the black
(Supplementary Table S1). tea fermentation quality index. The main motivation for testing clas-
sification algorithms with different properties (linear/non-linear) was
2.5.6. Software to solve complex pattern classification problems by selecting the op-
The algorithms were executed in MATLAB 2014b operating on timal decision. As presented in Table 2, the KNN, LDA, and SVM models
Windows 7, and mapping was performed using Origin 2017 (Origin correctly classified the samples, with classification calibration set ac-
Lab. Corp., Northampton, MA). curacies of 65.75%, 91.78%, and 89.04%, respectively, and prediction
set accuracies of 89.19%, 89.19%, and 94.59%, respectively. The effect
3. Results and discussion of KNN model calibration set was very poor, which might because the
sample of black tea fermentation degree was unbalanced, and KNN is
3.1. Data analysis of TP not good during training. Although LDA and SVM model achieved good
results, revealed that 9 characteristic values of R, G, B, H, S, V, L*, a*,
The changes in TP values during fermentation are presented in and b* based on computer vision were not sufficiently effective in
Table 1. During black tea fermentation, PPO and PO cause enzymatic evaluating black tea fermentation. This might due to black tea fer-
oxidation and polymerization of TP to form compounds that impart mentation involves chemical changes in addition to color changes.
flavor and special tastes to black tea. As presented in Table 1, during
preliminary fermentation, the average TP content of black tea was the 3.3. FT-NIR spectra information analysis
highest at 140.46 mg/g. After fermentation for 0.5–2.0 h, the TP con-
tent decreased significantly, which implies that TP were rapidly oxi- FT-NIR was used to assess the chemical information of the black tea
dized. At higher concentrations of TP, the enzyme reaction rate reduced fermentation process. Fig. 4 presents the raw spectra of the black tea
to maintain a maximum enzyme reaction rate (Muthumani & Kumar, fermentation samples, which recorded the FT-NIR spectra curve for
2007). After fermentation for 2.5–3.0 h, the degradation rate of TP was wavenumbers in the range 800 and 2500 nm. The spectrogram of the
in the range 21.35%–21.39% and the TP rarely degraded. At this time, four fermentation degrees depicts slight differences, which indicates
that during fermentation, the chemical components and the changes in
Table 1 surface color affected the FT-NIR spectral response characteristics of the
Effect of fermentation duration on TP content during black tea fermentation. samples (Porep, Kammerer, & Carle, 2015). In this study, standard
normal variate transformation (SNV) was applied for spectra pre-
Fermentation TP (mg/g) Average Standard TP Group
processing, eliminating the effect of uneven sample size on light pro-
time (h) (mg/g) deviation decline
(mg/g) rate pagation. Because of the redundancy in spectral data, PCA was used to
(%) reduce data dimensionality and to visualize the distribution of samples
during black tea fermentation. Fig. 5a shows the scores for the first two
0.0 126.47–153.78 140.46 7.45 0.00% Preliminary
PCs. The total variance explained by the first two components was
fermentation
0.5 126.47–162.18 138.28 12.58 1.56% Insufficient 97.14% (PC1 = 88.97% and PC2 = 8.17%). It can be seen that the four
1.0 118.49–158.40 127.82 11.88 9.00% fermentation fermentation stages are almost all separate. This indicates that FT-NIR
1.5 114.85–129.13 123.35 3.97 12.18% combined with PCA can effectively determine the degree of black tea
2.0 110.50–136.13 120.04 8.39 14.54% fermentation. The main spectral contributions observed in the loading
2.5 100.84–121.43 110.50 6.47 21.35% Moderate
3.0 97.48–126.05 110.42 10.29 21.39% fermentation
are shown in Fig. 5b. PC1 obtains information bands: 1350–1375 nm
3.5 97.48–111.76 104.20 4.94 25.82% Excessive (C–H group first-order frequency doubling) and 1800–1900 nm (C]O
4.0 93.28–109.66 100.42 4.79 28.51% fermentation bonds). As for PC2, the main contribution comes from the following
4.5 90.76–113.03 100.25 6.13 28.63% band 1380–1420 and 1900–2000 nm (O–H combination bands). During
5.0 87.39–105.46 100.88 5.34 28.25%
the fermentation of black tea, some important chemical changes, such
TP: Tea polyphenols. as catechins, amino acids, and tea pigments, are associated with the
Preliminary fermentation: 0 h. absorption peaks of the principal component load figure.
Insufficient fermentation: 0.5–2.0 h. The PCA-processed FT-NIR datasets were severally used as input
Moderate fermentation: 2.5–3.0 h. matrices for the KNN, LDA, and SVM models. The identification results
Excessive fermentation: 3.5–5.0 h. of the calibration and prediction sets are presented in Table 2. For the

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G. Jin, et al. LWT - Food Science and Technology 125 (2020) 109216

Fig. 3. Black tea images at different fermentation time: (a) preliminary fermentation, (b) insufficient fermentation, (c) moderate fermentation, and (d) excessive
fermentation.

Table 2
Comparison of discrimination accuracies obtained using KNN, LDA, and SVM
methods on different fermentation samples.
Model Data Parameters Correct classification rate
source
Calibration set Prediction set

KNN NIR k=3 90.41% 89.19%


CVS k=3 65.75% 89.19%
LDA NIR – 89.19% 86.30%
CVS – 91.78% 89.19%
SVM NIR c = 64, g = 2.2974 97.26% 89.19%
CVS c = 21.1121, 89.04% 94.59%
g = 0.143587

g: gamma.

KNN, LDA, and SVM models, classification calibration set accuracies


were 90.41%, 89.19%, and 97.26%, respectively, and prediction set
accuracies were 89.19%, 86.30%, and 89.19%, respectively. Compared
with the model of image information, the prediction set of SVM model
(non-linear) and LDA (linear) model correct classification rate is re-
duced. But, in the KNN model, the performance of FT-NIR was better
than that of CVS. This result indicating that the chemical information of Fig. 4. Raw spectral data of fermented black tea.
black tea fermentation is a very complex change, and the determination
of fermentation degree by a single sensor needs to be improved. sensor data to evaluate food quality is insufficient, and a combination of
multisensor data technology is necessary (https://www.sciencedirect.
com/science/article/pii/S0260877417301796 Borràs et al., 2015).
3.4. Data fusion based on FT-NIR and CVS signals
Therefore, in this study, two different mid-level fusion feature extrac-
tion methods were used to evaluate the black tea fermentation degree.
Black tea fermentation is complex and involves a change in color,
SPA was used to extract three characteristic wavelengths: 802, 945, and
aroma, and chemical composition; therefore, acquisition of single-

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G. Jin, et al. LWT - Food Science and Technology 125 (2020) 109216

Fig. 5. PCA of spectral data of fermented black tea. (a) Score plot; (b) Loading plot.

Table 3 was very beneficial to data fusion. For the SVM model, two methods of
Comparison of results of KNN, LDA, and SVM models of classification for dif- mid-level fusion feature extraction were compared. PCA feature ex-
ferent fermentation samples using different feature extraction methods at mid- traction is better than SPA and Pearson because the feature variables
level fusion. extracted using SPA and Pearson retain the features of most related
Model Feature extraction Parameters Correct classification rate variables; however, some variables that were not significantly corre-
lated were overlooked. More chemical information was characterized
Calibration set Prediction set by fusing spectral and image data collected during black tea fermen-
KNN SPA + Pearson k=1 80.82% 75.68%
tation. Spectral information explains the change in catechins (Dong
PCA k=1 87.67% 91.89% et al., 2017), and image information corresponds to generation of TFs
LDA SPA + Pearson – 98.63% 91.89% and TRs (Dong, Liang, et al., 2018), which are formed by TP degrada-
PCA – 95.89% 94.59% tion. Therefore, the black tea fermentation process can be compre-
SVM SPA + Pearson c = 512, 100.00% 94.59%
hensively evaluated by using FT-NIR and CVS.
g = 0.353553
PCA c = 6.9644, 100.00% 100.00% A low-level fusion method requires numerous sensors and extensive
g = 1.31951 data processing (Kiani, Minaei, & Ghasemi-Varnamkhasti, 2016), which
is time-consuming and difficult to apply in black tea fermentation.
However, because of the potential modern industrial applications of
1661 nm (Supplementary Fig. S1). Absorption at 802, 1661, and black tea, data must be processed and analyzed in real time. The mid-
945 nm can be attributed to N–H stretching (Wang et al., 2018), C–H level fusion method efficiently addresses this challenge because using it
and O–H stretching (Wu, Meng, et al., 2019), and Ar-OH stretching for feature extraction significantly reduces the data dimension. Mid-
(Leme et al., 2018), respectively. These characteristic wavelengths were level fusion methods achieved favorable results in the evaluation of
related to TP. A Pearson correlation analysis was used to extract cor- black tea fermentation because the fusion of data from complementary
relation variables H, S, a*, and b*. Data from these two different sensors sensors responded to different characteristic phenomena and increased
were combined into a single matrix for a multivariate analysis. As the correct classification rate (Rosa et al., 2017). Real-time monitoring
presented in Table 3, in the SPA and Pearson feature extraction model, of black tea fermentation should consider the possibility of rapid data
the calibration set accuracies of KNN, LDA, and SVM were 80.82%, processing and automated online monitoring of industrial-scale opera-
98.63%, and 100%, respectively, and the prediction set accuracies were tions. The data fusion method offers a viable solution, and con-
75.68%, 91.89%, and 94.59%, respectively. Adulterated olive oil (Li, temporarily, the results are encouraging; however, urgently designing a
Xiong, & Min, 2019) for SPA extraction of characteristic spectral in- portable NIR and a low-cost camera is necessary because of their por-
formation fusion did not improve the model. However, in this study, table applications.
SPA spectral information feature extraction and integrated image in-
formation improved model performance. It may be that integrating
multiple different types of sensors more effective than a single spectral 4. Conclusion
data fusion. In PCA feature extraction modeling, calibration set ac-
curacies of KNN and LDA were 87.67% and 95.89%, and prediction set In this paper, we demonstrated the feasibility of applying FT-NIR
accuracies were 91.89% and 94.59%, respectively. PCA of SVM was the and CVS technologies for evaluating black tea fermentation. The results
most effective with calibration and prediction set accuracies of 100% present that the mid-level fusion method is better in evaluating the
each. After the fusion of multiple-information, compared with FT-NIR black tea fermentation degree than using a single feature, implying that
data, the performance of the KNN model has not improved, suggesting information obtained from an individual sensor is insufficient. In ad-
that the model after data fusion may not necessarily improve. For the dition, the PCA and SPA and Pearson mid-level fusion feature extraction
LDA model, the prediction set results are improved in both middle level methods were compared. The mid-level fusion SVM model based on
data fusion. In addition, the SVM model obtained the best classification PCA feature extraction had the highest classification accuracy, and the
results. SVM is recognized as the best method for solving classification calibration and prediction set accuracies were 100% each. The results
problems by projecting data into high dimensions, and constantly of this study provided a theoretical basis to automate the black tea
seeking optimal classification (Ouyang, Zhao, & Chen, 2014), which fermentation process. In the future, a portable NIR device and a simpler
CVS at the mid-level need to be developed to automate the monitoring

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G. Jin, et al. LWT - Food Science and Technology 125 (2020) 109216

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