Best in class
MACSQuant® Instrument quick guide
Start-up, shutdown, maintenance
Instrument start-up
1. Ensure the MACSQuant® Instrument main power switch
is in the on-position ‘I’.
2. Tap the touchscreen monitor to activate the
MACSQuantify™ Software.
3. After logging on to MACSQuantify Software, the
instrument is in data analysis mode.
4. Prime the instrument (power the lasers, activate fluidics)
by clicking the icon and choose ‘Acquisition mode’
(figure 1).
5. Optical bench requires at least 30 minute warm up
before any fluorescence measurement can be made.
 Note: While the instrument is warming, it is best
practice to perform a flush cycle followed by a clean.
Refer to section ‘Rinse, flush, and clean programs’
below.
6. Instrument can be monitored by the status bar
(figure 2) or hardware monitor (figure 3).
For detailed information refer to the user manual.
Figure 1: Priming instrument
Figure 2: Instrument status bar indicating the MACSQuant Instrument
is in data analysis mode (top) or acquisition mode (bottom)
Instrument shutdown
1. 
The MACSQuant Instrument can be configured
to automatically power down the lasers by
pre-selecting a time under
Edit > Options (default) > Timers > Standby Timer.
2. 
To manually shutdown the instrument from acquisition
mode, click the icon.
Choosing ‘Instrument off’ will initiate a fluidic washing
3. 
cycle, fill fluidics with storage solution, power down
the lasers, and shutdown the MACSQuantify Software.
Choosing ‘Data Analysis Mode’ will initiate a fluidic
4. 
washing cycle, fill fluidics with storage solution, and
power down the lasers. The software will stay active
for data analysis or other tasks.
5. 
Upon full instrument shutdown, the monitor will be
in stand-by mode, until the touchscreen is tapped.
This will initiate the MACSQuantify Software to restart.
Figure 3: Monitoring of optical bench under View > Hardware
Basic maintenance of the
MACSQuant® Instrument
MACSQuant® Buffers and solutions
Three fluidic containers are housed on the MACSQuant
Instrument to supply MACSQuant Buffers to the instrument.
A fourth container collects the waste.
Running Buffer (# 130-092-747)
Sterile, ready-to-use buffer with preservative.
Used as sheath fluid, buffer A or B, and during
‘Rinse’ program.
Washing Solution (# 130-092-749)
Sterile, ready-to-use basic solution
containing detergent. Used during ‘Flush’
and ‘Shutdown’ programs.
Storage Solution (# 130-092-748)
Sterile, ready-to-use solution. Used for
overnight and long term storage of the
instrument. Automatically fills fluidics
upon instrument shut down.
Waste collection bottle
If measuring biohazardous material, it is
recommended to add 100 mL of MACS Bleach
Solution (# 130-093-663) to waste container.
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MACS, the MACS logo, MACSQuant, and MACSQuantify are registered trademarks or trademarks of Miltenyi Biotec GmbH. Unless otherwise specifically indicated,
Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use. Copyright © 2014 Miltenyi Biotec GmbH. All rights reserved.
Rinse, flush, and clean programs
The MACSQuant Instrument has several rinsing programs
to clean fluidics between samples, to remove partial clogs,
or to decontaminate fluidics after running biohazardous
material.
Rinse program
For washing of fluidics after a poor quality sample
(aggregates, dead cells) a rinse program can be chosen by
clicking the icon on the status bar. This program uses
the MACSQuant Running Buffer.
Flush cycle
For clearing fluidics of partial or full clogs a flush cycle can
be used by right-clicking the icon on the status bar and
choosing ‘Flush’. This 16’ program uses the MACSQuant
Washing Solution to incubate the flow cell and back flush
the solution through the tubing system.
Clean program
For decontamination of the instrument, a clean cycle can
be performed by right-clicking the icon on the status
bar and choosing ‘Clean’. The program can also be used
to remove clogs in combination with a Flush cycle. This
10’ process uses on-screen instructions to walk the user
through the procedure. A tube with 0.5 mL of MACS Bleach
Solution is required.
Periodic maintenance
Column exchange (optional)
Exchange the MACSQuant Column (# 130-094-458)
every three months. For detailed information refer
to the user manual.
Hydrophobic air filter exchange
Exchange filters when they come in contact with liquid,
and at least once a year.
Cleaning of uptake needle
The instrument should be turned OFF before needle
cleaning. To prevent contamination or blockages, wipe
needle with tissue soaked with 70% ethanol.
Are you in need of additional assistance?
Visit www.miltenyibiotec.com/locations to find
your nearest Miltenyi Biotec contact. 130-106-087