= PYNR TELANGANA VETERINARY UNIVERSITY ‘COLLEGE OF VETERINARY SCIENCE: HYDERABAD ~s00 030 DEPARTMENT OF VETERINARY MIC No, of credits: 5 (3+2) ROBIOLOGY No. of Units: 5 THEORY COURSE OUTLINES UNrT-1 (GENERAL & SYSTEMATIC VETERINARY BA 1 Tntroduction and History of Microbiology 7 2. | Classification and nomenclature of ; = porta Sage licroscopy : Principles of tmicroscopy - Types of microscopes (Bright field, dark { field, fuorescent, phase contrast, confocal & electron micr aes. ast, confocal & electron microscope) and Micror cytoplasmic membrane, capsule fimbriac, flagella, nuclear body and ribosomes) Endospore - sporulation and germination 6.__| Growth and nutritional requirements of aerobie and anaerobie bacteria TRE. [ Normal, opportunistic and saprophytic bacterial Hora: Types and sources oF infection, mode of transmission, pathogenicity, virulence, detemnianants of| virulence; Epizootic and enzootic diseases; Bacteraemia, Septicaemia and Toxaemia; Bacterial toxins: Endotoxins and Exotoxins ‘9, [Bacterial genetics (Mutations, Transformation, Transduction and Conjugation); I | Plasmids [0 TaAntiione “Treatment and Contol of bacterial infections [Study of the important ‘pathogenic bacteria in relation to their morphology, isolation, cultural and | amtigenic characters; epidemiology and pathogenesis; diagnosis, prevention and control of | diseases caused by the following genera. | 11&12, | Gram positive covet | Genus: Staphylococcus spp: Saureus, S.hyieus & S.intermedius — | Genus: Sireptocoeeis”spp: S pyogenes, S.zooepidemicus, S.equi, ‘S.equisimilis, S.canis, Mastitis Streptococci (S.agalactae, Suberis & S.dysgalactiag) TTrregular, nonsporing Gram positive rods | Genus: Corynebacterium spp: C.pseudotuberculosis, C.rens Truperella cs Rhodococcus equi (C-equi) Tid} Regular, non sporing Gram positive rods ~ Genus: Exysipelothrix spp: E.rhusiopathiae “ Genus: Listeria spp: L.monocytogenes / 3} Endospore forming Gram positive rods es Coy 75. - (17. | Genus: Bacillus spp: Banthracs and other anthracoid organisms iw Genus: Clostridium spp: C.perfringens, C.chauvoei, Csepyicim, Conovsi, Cage ox | & Chotulinum 5 Tat Genus: feabacerum sp Novis, Maa epmpex, Magn subsp. 7 _| paratuberculosis 75. | Genus: Actinomyces sp: A.bovis U7 Genus: Nocardia sp: N. asteroides. Genus: Streptomyces cy \ | Genus: Dermatophilus sp: D.congolensig_~_ “59,1 | Facultative anaerobic Gram ra ods; Family : Enterobacteriaceae 21822, | Genus: Escherichia sp: E.coli ; \_-7 | Genus: Salmonella spp: ‘S.choleraesuis serovars: S.ty} S.pullorum, S.galilnarum : Klebsiella sp: K.pneumoniae pphimurium, S.abortus equi,| Genus; Proteus spp: P.vulgari, P.mirabilis _| Genus: Yersinia spp: Y.cnterocolitica, Y.pseudotubergulo Gaus aeruginosa, — Burkholderia Y-pestis mallet (P.mall jens: P ‘Actinobacillus ligni i : Haemophilus parasuis, Avibacterium paragallinarum (H.paragallinarum) Brucella spp: Babortus, B.suis, it 3132, | Leptospira and other Spirochaetes Genus: Leptospira spp: L.bif_exa, L.interrogans serovars — I cterohaemorthagi Canicola, Pomona, Hebdomadis, Grippotyphosa Genus: Borrelia spp: B.anserina | Mycoplasmas or Mollicutes | Genus: Mycoplasma spp: M.mycoides, M.bovis, M.agalactiae, M.capricolum, | Mcapri, M.hyopneumoniae, M.hyothinis, M.hyopneumoniae, M. gallisepticum, | M.gallinarum, M.synoviae, M.meleagridis M.canis, | 34833. | Rickettsia and Chlamydia \_/ | Genus: Rickettsia spp: Rurickettsii Genus: Ehrlichia spp: E.canis Sf Genus: Cowdria spp: C.ruminantium Genus: Neorickettsia spp: N.helminthoeca Genus: Chlamydia spp: C.trachomatis, 36. ‘Other pathogens :Genus: Coxiella sp: C.burnetii 37. _ | Emerging , re-emerging and transboundary bacterial pathogens 1 3. Veterinary \ 4, Clinical Veterinary Microbiology by B.K. Markey, Reference books: a 1. Microbiology by Michael J.Pelezar, E.C.S. Chan and Noel R.Krieg (5" edition) 2. Bergey's Manual of Determinative Bacteriology by J.G. Holt and N.R.Krieg (9° Edition) 1994 ES. FitzPatrick, S.Fanning, and Pt 1 (2"* edition) Cullinane and D. Magu 5, Animal Microbiology by A. Buxton and G, Fraser (Volume 1) UNIT- I: GENERAL & SYSTEMAT | oaerophilic, motile, helical /vibrioid Gram negative bacteria i ~~ __| Genus: Campylobacter spp: C fetus. 1 | 29. Genus: Bordetella sp: B-bronchisepica ~ ~ | | Genus: Moraxella sp: M.bovis | 30) | Gram negative, Anaerobic, straight, curved and helical bacteria | | L Genus: Dichelobacter nodosus (Bacteroides nodosus) | L Genus: Fusobacterium spp: F.necrophorum | | 38. | a. Introduction, elassificat bb, Growth and nutritional requirements of c, Reproduction in Fungi ‘elation to their morphology, Holation, growth, Study of follow! 's, epidemiology, pathogenesis, diagnosis and cultural, biocher cies lowing a iia Mucor, RRS 39. fa —Rspersillus, Peneillium - = a sick (Trichophyton, Microsporam, Epdermaphyion) and Malasseda | see cmphie fungi ~ Sporolrichum, Blastomyces, Coecidioides and Histoplasma spp a2. - a a": MICROBIAL BIOTECHNOLOGY. M z Introd jm y and jis applications] | aT Gone nbinant DNA technology and is application vectors and expression vectors; transformation and Reference bool 1. Molecular Cloning by 2. Concepts in Biotechnology by D. Balasubrama Green, Kunth in (Universities press). UNIT IY: 56, _5t__|hom 58 Si | Tapes of nmi = id organs, tissues and Cells 7) y - RU ok iz Antigens (T dependent, 1 ns, heterophile antigens, cross reacting | antigens, blood group antigens), hapten, epitope, specificity, mitogens and factors ffecting immunogenicity; Adjuvants. ‘Antibody: Structure, physiochemical properties and functions of various classes of od Ockrtoxeoels immunoglobulins «_ : | Theories of antibody produetion- ‘Major histocompatability complex (MHC) structure, function and gene organization. ‘Antigen processing and presentation _ Hybridoma and monoclonal antibodies _~ 5 67. plement system: activation pathways ‘and biological consequences 7 @. | Cytokines: general properties, major types and function, 7 ©. Tiypersenstiviy: classification and mechanism of induction <7 70. ‘Autoimmunity; | Tmmunotolerance V7 _J Ti. | Goncept oF Immunity 1 Microbes, Vaccines and other biologicals. Reference books: jenni Punt, Sharon A. Stranford, Patricia P. Jones I. Kuby Immunology by Judith A. Owen, (7* edition). 2. Veterinary Immunology by Ian R.Tizard (9” edition). 3. Roit's essential Immunology by Ivan M.Roitt & Peter J. Delves (11 4. Veterinary Immunology ~ Basic Concept and Applicatifons by Y. aba ( ) 2006. edition).73._| Classification of viruses |_ 74, Structure and composition of viruses, ; | Viral Replication _ 76. __| Cultivation of viruses — Assay methods; Purification of viruses 77. | Genetic and” Non-genetie viral interactions; Virus-Cell interactions; Via | Pathogenesis - Inclusion bodies ~ Interferon, Oncogenesis, Latency and immunopathology ~ Prevention and Control of viruses ~ Antiviral agents. Studies ‘on General properties, Antigens, Cultivation, Pathogenesis, Epidemiotogy, Cli signs, Diagnosis, Prevention and Control of the following viruses and Prions causing diseases Livestock and Poultry. RNA Viruses l_ Birnavirida :80, | Reoviridac Rotavirus, Bluctongue virus, African horse sickness viru 81882 | Paramyxovi ‘Newcastle disease virus, Canine Distemper virus, PPR virus Physico-chemical properties of Viruses 83._ | Rhabdoviridae 8, Ephemeral fever virus, Vesicular stomatitis virus | [8 | Boravirdse 83,__| Onthomyxoviridae (86. —TCoronaviridae | 87._| Artriviridae ‘88889. | Picomavitidae | Foot and mouth disease virus; Diflerential diagnosis of EMD | with other vesicular dsease(Swine vesicular dscase vzus, | __| vita epi vis, Avian encephalomyti [90 eae sie ean 91. | Togavi 92, | Flaviviridae frac jrea erences Jeucosis virus, Bovine leukemia virus, Bo virus ne immunodeficieney DNA viruses Poxviridae Capripox virus, Avipox virus, Cowpoxvirus, Vace Lumpy: 0x Virus, Parapox virus (Ort) Herpesviridae | Bovine herpesviruses (IBRV); Equine Herpesvinuses (Equine | abortions, Equine coitalexanthema); Gallid Herpesvirus (LTV, eames | Herpesvirus (Pseudorabies virus) Alephaline Herpesvitus | ovcrv) Infectious Canine Hepats vis, gg drop Some vis Fowl adenovirus, lclsion body heats Hydropercaiam syndrome Papillomatosis, Bovine &Equine Papillomaviruses Canine parvovins, Feline Pareucopena vis, Paring Papovaviridae ‘Spongiform Encephalopathies of animal; Scrape, Bovine Spongiform Encephalopathy ‘Other viral agents; Emerging , re-emerging and transboundary viruses & viral infections Reference Books ' Fenner's Veterinary Virology by N, James MacLachlan and Edward J Dubovi (4° Edition) 2011.