Fluorescence microscopy results

Test Patient results Normal range

ANA Test Negative Positive >1/20

Negative<1/20
 Fluorescence microscopy results
Liver screen test

Test Patient results Normal range

ANA Test Negative Positive >1/20

Negative<1/20

Anti smooth antibody Negative

Anti LKM Negative

Anti mitochondrial antibody Negative

 Fluorescence microscopy results

Test Patient results Normal range

Anti parietal cell antibodies Negative Positive >1/20

Negative<1/20

ANA fluorescence microscopy report

.The test is weakly positive

.On morphological base, the pattern cannot well be determined

 ANA fluorescence microscopy report

.The test is positive

On morphological base, the pattern is most probably: AC5

.AC5: Nuclear Pattern, course speckled

Titer: 1/40

Target Antiges: hnRNP, U1-RNP, SM & & RNA polymerase 111

Clinical association: systemic lupus erythematosus & connective tissue

.disease
 ANA fluorescence microscopy report

.The test is weakly positive

Pattern: Nuclear AC1

Titer: 1/40

.AC1: Homogenous

Traget Antigens: dsDNA, Nucleosomes & histon

.Clinical association: SLE or Drug induced SLE

 ANA fluorescence microscopy report

.The test is positive

Pattern: Nuclear AC1 or AC4

.AC1: Homogenous

Traget Antigens: dsDNA, Nucleosomes & histon

.Clinical association: SLE or Drug induced SLE

.AC4: Fine Speckled

Target Antiges: Mi-2

.Clinical association: Dermatomyositis & idiopathic Myelositis

 ANA fluorescence microscopy report

.The test is positive

Pattern: Nucleolar AC8 & 9

Target Antiges: PM-Scl, U3-nRNP/fibrillarin

Clinical association: Polymyositis/ Dermatomyositis overlap syndrome &

.progressive systemic sclerosis
 ANA fluorescence microscopy report

.The test is positive

Pattern: Nuclear AC4

.AC4: Fine Speckled

Target Antiges: Mi-2, Ku

Clinical association: Poly/Dermatomyositis, progressive systemic

sclerosis & systemic lupus erythematosus
 ANA fluorescence microscopy report

.The test is positive

On morphological base, the pattern is most probably: AC19 or 21

.AC19 or 21: Cytoplasmic pattern

Titer: 1/80

Target : SRP Ag, Ribosomal P or to less extend Mitochondrial antibody

Clinical association: Autoimmune myositis, SLE or to less extend

.primary biliary cirrhosis
 Fluorescence microscopy results

Test Patient result Normal range

Anti Smooth Ab. Negative Positive >1/20

Negative<1/20

Anti Soluble Antigen Negative Positive >1/20

Negative<1/20
 Fluorescence microscopy results

Endomysial Antibody: Negative.

.Antigliadin antibodies: Negative

Fluorescence microscopy results

Test Patient results Normal range

ANA Test Negative Positive >1/20

Negative<1/20
Anti u1 RNP Negative

Anti Centromere Negative

Anti double strand DNA Negative Positive >1/20

Negative<1/20

Anti smith antibody Negative

Anti-pm/scl antibody Negative

Anti Jo Antibody Negative

 ANA fluorescence microscopy report

.The test is positive

Titer: 1/80

On morphological base, the pattern is most probably: AC5

Nuclear Pattern: AC5

.AC5: Course speckled

Target Antiges: hnRNP, U1-RNP, SM & & RNA polymerase 111

Clinical association: systemic lupus erythematosus & connective tissue

.disease
 ANA fluorescence microscopy report

To less extend the pattern: AC4

Nuclear Pattern: AC4

.AC4: Fine Speckled

Target Antiges: Mi-2, Ku

Clinical association: Poly/Dermatomyositis, progressive systemic

sclerosis & systemic lupus erythematosus
 ANA fluorescence microscopy report

.The test is positive

On morphological base, the pattern is most probably: AC9 or 10

.AC9 or 10: Nucleolar pattern

Titer: 1/80

Target Antiges: SCL-70, RNA Polymerase 1, U3-nRNP/Fibrillarin &

NOR-90

.Clinical association: Progressive systemic sclerosis

Note The DNA double strand will be repeated by the fluorescence microscope
.method for further evaluation & the result will be given to the patient later on
 ANA fluorescence microscopy report

.The test is positive

Pattern: Nuclear AC20 or to less extend AC18

AC20

Traget Antigens: Jo-1

. Clinical association: Polymyositis or SLE

AC18

Traget Antigens: Lysosomes & GWB proteins

.Clinical association: Neurological disease, some time in normal persons

 Fluorescence microscopy report

.The ANA test is positive

Pattern: AC6

AC6: Ant-Multiple nuclear dots

Target Antigens: Sp100, Sp140, PML, SUMO and MJ/NXP-2

.Clinical association: Primary biliary cholangitis

Anti smooth antibody test is negative.

Note: The sample has been received from outside the lab
 ANA fluorescence microscopy report

The test on fluorescent microscopical based is equivocal in the

.intensity of the color and not conclusive

ANA fluorescence microscopy report

.The test is weakly positive

On morphological base, the pattern is most probably: AC19

Cytoplasmic Pattern: AC19

.AC5: Fine speckled

 Titer: 1/40

Target Antigens: PL7, PL12, ribosomal P protein & SRP

.Clinical association: Myositis & systemic lupus erythematosus

 ANA fluorescence microscopy report

.The test is positive

On morphological base, the pattern is AC23

.AC23: Cytoplasmic pattern , Rings & Rods

Titer: 1/80

Target Antiges: Autoantigen inosin monophosphate dehydrogenase 2

Clinical association: HCV patients post-IFN/ribavirin therapy, rare in

SLE, Hashimoto`s and healthy controls
 ANA fluorescence microscopy report

.The test is weakly positive

.Pattern: Nuclear, Homogenous, but non-specific

Titer: 1/40

.Clinical association: Autoimmune disease

 Fluorescence microscopy results

Myasthenia gravis antibodies tests:

Heart & skeletal muscle antibody is negative.

 Fluorescence microscopy results
Sample: CSF

Test: NMO antibody

.Result: Negative

Fluorescence microscopy results

.Sample: Blood

Test: Anti aquaporin-4

.Result: Negative