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    PCR (polymerase chain reaction) is a laboratory technique used to amplify a single or few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. It uses thermal cycling, consisting of cycles of repeated heating and cooling of the DNA melting and annealing of primers to the DNA followed by primer extension with DNA polymerase. This allows the exponential amplification of the target DNA sequence. Some applications of PCR include DNA fingerprinting, diagnostics of genetic diseases, clone DNA for sequencing or expression of genes.

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    PCR (polymerase chain reaction) is a laboratory technique used to amplify a single or few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. It uses thermal cycling, consisting of cycles of repeated heating and cooling of the DNA melting and annealing of primers to the DNA followed by primer extension with DNA polymerase. This allows the exponential amplification of the target DNA sequence. Some applications of PCR include DNA fingerprinting, diagnostics of genetic diseases, clone DNA for sequencing or expression of genes.

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    11 views1 page

    3.5 PCR Virtual Lab

    Uploaded by

    William Ryu
    PCR (polymerase chain reaction) is a laboratory technique used to amplify a single or few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. It uses thermal cycling, consisting of cycles of repeated heating and cooling of the DNA melting and annealing of primers to the DNA followed by primer extension with DNA polymerase. This allows the exponential amplification of the target DNA sequence. Some applications of PCR include DNA fingerprinting, diagnostics of genetic diseases, clone DNA for sequencing or expression of genes.

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    Unit 3 - Genetics

    3.5.2 PCR
    Virtual Lab
    http://learn.genetics.utah.edu/content/labs/pcr/

    Read the intro “What are these things doing in my PCR?” and answer the questions below.

    1. What are primers? How many are used? Where do they attach? Why are they
    necessary?
    2. What is DNA polymerase? What does it do? Where do we get the DNA polymerase
    that is used in PCR? Why can’t we use human DNA polymerase?
    3. What are nucleotides? Why do we need to add them to the mixture? What happens
    to them?

    Begin the virtual lab.

    4. What does PCR stand for and what is it used for?


    5. Where could you extract the DNA sample?
    6. PCR tubes are designed for…
    7. Name all the reagents that are placed in the PCR tube.
    8. What is the name of the machine that performs PCR?
    9. At what temperature do the DNA fragments separate? What enzyme normally does
    this in our cells?
    10. Why do the primers find their complementary sequence before the DNA strand
    comes back together? What temperature is the thermocycler at now?
    11. How is the DNA polymerase activated?
    12. Summarize the three steps that are repeated to copies of a DNA sequence.
    13. During which cycle does your desired strand actually appear?
    14. After 30 cycles how many DNA strands do you have?

    What are some specific applications of PCR?

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