DUPLEX OLIGONUCLEOTIDE

Technical Data Sheet (TDS) For Research Use Only

YOUR ORDER SON DATE

0465L016084 1000853927 *1000853927* 8-Nov-17

CUSTOMER ADDRESS / INSTITUTION
BIANCO ALBERTO FRANCE STRASBOURG CEDEX 67084
OLIGO NAME BATCH SCALE CHEMISTRY BASES
1 6469668 1000 nmol RNA 23
SEQUENCE (5’ 3’) * Phosphorothioate linkage
1 GAA-CAG-GUC-GGA-CAU-CAC-CUU-55

OLIGO NAME BATCH SCALE CHEMISTRY BASES

1BIS 6469669 1000 nmol RNA 23
SEQUENCE (5’ 3’) * Phosphorothioate linkage
1 AAG-GUG-AUG-UCC-GAC-CUG-UUC-55

BACKBONE (BASES + LINKAGES)

A 6 4 MW (g.mol-1)1 8111.1 7297.5 Quantity

C 6 5
G 5 6 Ext. coeff. 236400 224800
U/T 4/0 6/0 (l.mol-1.cm-1)1
Other 2 2 (nmol) 48.0
1
s
Wobbles 0 0 Theoretical values (µg) 739.61
MODIFICATION(S) OLIGO OLIGO
Mod 3’ Mod 3’
Mod 5’ ATTO 647N 807.62 Mod 5’
Mod 1 0 Mod 1 0
Mod 2 0 Mod 2 0
Mod 3 0 Mod 3 0
Mod 4 0 Mod 4 0
Mod 5 dT 304.20 2 Mod 5 dT 304.20 2
Mod 6 0 Mod 6 0
Mod 7 0 Mod 7 0
Mod 8 0 Mod 8 0
Mod 9 0 Mod 9 0
Mod 0 0 Mod 0 0
PURIFICATION(S)
HPLC-RP
ADDITIONAL SERVICE(S)
FORMAT Dried ALIQUOTING
Number of Aliquots 0

QUALITY CONTROL(S)
MS Passed MS Passed
DELIVERY CONDITION
Room Temperature
RECONSTITUTION
To Make up to 100 µM concentration add 480 µl of recommended buffer.
To Make up to 20 µM concentration add 2.40 ml of recommended buffer.
To Make up to 5 µM concentration add 9.60 ml of recommended buffer.
LONG-TERM STORAGE
DNA -20 °C / RNA -70 °C
COMMENTS
General:
Specific: Annealing buffer used is 50 mM TRIS-HCl, 100 mM NaCl, pH 7.6

HELP EUROPE NORTH AMERICA

If you have any questions feel free to call our Oligo Centre depending on your location: If you have questions, please contact our Technical
[Belgium, France, Germany, Luxembourg, The Netherlands, United Kingdom, Switzerland, Support Team at +1 858 793 2661 or send an e-mail to
Austria] 00 800 666 00 123 (European toll free number), or send an email to ivd.na@egt-biotech.com
oligo@eurogentec.com
Kaneka Eurogentec S.A. Liège Science Park - Rue Bois Saint-Jean 5 - 4102 SERAING BELGIUM EGT North America, Inc.
Tel: +32(0)4 372 74 00 - Fax: +32(0)4 264 07 88 E-mail: info@eurogentec.com 34801 Campus Drive - Fremont, CA 94555
RPM Liège T.V.A.-(BE)-0427.348.346 - Web: www.eurogentec.com Tel.: +1 (510) 896 -1350 * Fax: +1 (510) 791-9572
E-mail: ivd.na@egt-biotech.com * Web: www.egt-biotech.com
Ber CTDS Annealing 11.rtf
 DUPLEX OLIGONUCLEOTIDE
Technical Data Sheet (TDS) For Research Use Only
YOUR ORDER SON DATE

0465L016084 1000853927 *1000853927* 8-Nov-17

CUSTOMER ADDRESS / INSTITUTION
BIANCO ALBERTO FRANCE STRASBOURG CEDEX 67084
OLIGO NAME BATCH SCALE CHEMISTRY BASES
2 6469670 1000 nmol RNA 23
SEQUENCE (5’ 3’) * Phosphorothioate linkage
1 AAU-CAC-GGC-AGC-CAG-GUC-CUU-55

OLIGO NAME BATCH SCALE CHEMISTRY BASES

2BIS 1229378 1000 nmol RNA 23
SEQUENCE (5’ 3’) * Phosphorothioate linkage
1 AAG-GAC-CUG-GCU-GCC-GUG-AUU-55

BACKBONE (BASES + LINKAGES)

A 5 4 MW (g.mol-1)1 8087.1 7336.5 Quantity

C 7 5
G 5 7 Ext. coeff. 230100 222200
U/T 4/0 5/0 (l.mol-1.cm-1)1
Other 2 2 (nmol) 50.0
1
s
Wobbles 0 0 Theoretical values (µg) 771.18
MODIFICATION(S) OLIGO OLIGO
Mod 3’ Mod 3’
Mod 5’ ATTO 647N 807.62 Mod 5’
Mod 1 0 Mod 1 0
Mod 2 0 Mod 2 0
Mod 3 0 Mod 3 0
Mod 4 0 Mod 4 0
Mod 5 dT 304.20 2 Mod 5 dT 304.20 2
Mod 6 0 Mod 6 0
Mod 7 0 Mod 7 0
Mod 8 0 Mod 8 0
Mod 9 0 Mod 9 0
Mod 0 0 Mod 0 0
PURIFICATION(S)
HPLC-RP
ADDITIONAL SERVICE(S)
FORMAT Dried ALIQUOTING
Number of Aliquots 0

QUALITY CONTROL(S)
MS Passed MS Passed
DELIVERY CONDITION
Room Temperature
RECONSTITUTION
To Make up to 100 µM concentration add 500 µl of recommended buffer.
To Make up to 20 µM concentration add 2.50 ml of recommended buffer.
To Make up to 5 µM concentration add 10.00 ml of recommended buffer.
LONG-TERM STORAGE
DNA -20 °C / RNA -70 °C
COMMENTS
General:
Specific: Annealing buffer used is 50 mM TRIS-HCl, 100 mM NaCl, pH 7.6

HELP EUROPE NORTH AMERICA

If you have any questions feel free to call our Oligo Centre depending on your location: If you have questions, please contact our Technical
[Belgium, France, Germany, Luxembourg, The Netherlands, United Kingdom, Switzerland, Support Team at +1 858 793 2661 or send an e-mail to
Austria] 00 800 666 00 123 (European toll free number), or send an email to ivd.na@egt-biotech.com
oligo@eurogentec.com
Kaneka Eurogentec S.A. Liège Science Park - Rue Bois Saint-Jean 5 - 4102 SERAING BELGIUM EGT North America, Inc.
Tel: +32(0)4 372 74 00 - Fax: +32(0)4 264 07 88 E-mail: info@eurogentec.com 34801 Campus Drive - Fremont, CA 94555
RPM Liège T.V.A.-(BE)-0427.348.346 - Web: www.eurogentec.com Tel.: +1 (510) 896 -1350 * Fax: +1 (510) 791-9572
E-mail: ivd.na@egt-biotech.com * Web: www.egt-biotech.com
Ber CTDS Annealing 11.rtf