EXPERIMENT 9:

VITAMIN
ANALYSIS
VITAMINS
Organic compound essential for proper
body functioning.
Obtained from the dietary sources
Cofactor in conjugated enzymes

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VITAMINS
Two classes of Vitamins:
• Water-soluble Vitamins
• Fat (lipid) soluble Vitamins

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1. WATER-SOLUBLE VITAMINS:
Vitamin C (Ascorbic acid)
Vitamin B Complex: (B1 to B12)
• Vitamin B1 (Thiamine)
• Vitamin B2 (Riboflavin)
• Vitamin B3 (Niacin)
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2. FAT-SOLUBLE VITAMINS:
Vitamin A (Retinol, β-carotene)
Vitamin D (Calciferol)
Vitamin E (Tocopherol)
Vitamin K (Phylloquinone/
Menaquinone)

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EXPERIMENTAL
RESULTS:

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A. PREPARATION FROM
COMMERCIALLY-PREPARED
VITAMINS
CCl4 (Carbon Tetrachloride) is used to
dissolve fat-soluble Vitamins (A, D, E).
A nonpolar solution.
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 B. ORGANIC SOURCES
Carrots – source of Vitamin A
Fish liver oil – source of Vitamin D
Coconut oil – source of Vitamin E
Yeast – source of Vitamin B3 (Niacin)
Tomatoes – source of Vitamin C

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 C. VITAMIN A ANALYSIS
SbCl3 (Antimony Trichloride) in
Chloroform is used to detect Vitamin A
or carotenoids.
Colorimetric method: “Carr-Price
Method”
Positive result: Blue-colored complex
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 D. VITAMIN D ANALYSIS
Pyrogallol in Benzene – used to dissolve
Vitamin D.
AlCl3 (Aluminum Chloride) – inhibits
Vitamin D – dependent Calcium
absorption.

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 D. VITAMIN D ANALYSIS
Principle: Aluminum will form a complex
with Vitamin D, inhibiting it from being
absorbed by the body.
Result: Yellow solution with white
precipitate

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 E. VITAMIN E ANALYSIS
Nitric acid in alcohol is a very explosive
solution.
Positive result: Formation of 2 layers.
Top layer – orange-colored solution
Bottom layer – yellow –colored solution

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 F. NIACIN ANALYSIS
NaOH – used to neutralize the solution
Copper (III) Sulfate – used to dissolve
Vitamin B
Result: Deep blue solution

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 G. VITAMIN C ANALYSIS
Phosphomolybdic acid is reduced into
Molydenum Blue by Ascorbic acid.
Color change is directly proportional to
the amount of Vitamin C present.
Result: Blue-colored complex

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