Bioinformatics-II (BIF501)

Assignment No.02
Student id mc180204524

1. Retrieve the DNA sequence of your candidate gene.

HBB hemoglobin subunit beta [ Homo sapiens (human) ]

>NC_000011.10:c5227071-5225464 Homo sapiens chromosome 11, GRCh38.p13 Primary

Assembly
ACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGA
GGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGC
AGGTTGGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAAACTGGGCATGTGGAGACAGAGAAG
ACTCTTGGGTTTCTGATAGGCACTGACTCTCTCTGCCTATTGGTCTATTTTCCCACCCTTAGGCTGCTGG
TGGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGATGCTGTTATGGG
CAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTGGAC
AACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACT
TCAGGGTGAGTCTATGGGACGCTTGATGTTTTCTTTCCCCTTCTTTTCTATGGTTAAGTTCATGTCATAG
GAAGGGGATAAGTAACAGGGTACAGTTTAGAATGGGAAACAGACGAATGATTGCATCAGTGTGGAAGTCT
CAGGATCGTTTTAGTTTCTTTTATTTGCTGTTCATAACAATTGTTTTCTTTTGTTTAATTCTTGCTTTCT
TTTTTTTTCTTCTCCGCAATTTTTACTATTATACTTAATGCCTTAACATTGTGTATAACAAAAGGAAATA
TCTCTGAGATACATTAAGTAACTTAAAAAAAAACTTTACACAGTCTGCCTAGTACATTACTATTTGGAAT
ATATGTGTGCTTATTTGCATATTCATAATCTCCCTACTTTATTTTCTTTTATTTTTAATTGATACATAAT
CATTATACATATTTATGGGTTAAAGTGTAATGTTTTAATATGTGTACACATATTGACCAAATCAGGGTAA
TTTTGCATTTGTAATTTTAAAAAATGCTTTCTTCTTTTAATATACTTTTTTGTTTATCTTATTTCTAATA
CTTTCCCTAATCTCTTTCTTTCAGGGCAATAATGATACAATGTATCATGCCTCTTTGCACCATTCTAAAG
AATAACAGTGATAATTTCTGGGTTAAGGCAATAGCAATATCTCTGCATATAAATATTTCTGCATATAAAT
TGTAACTGATGTAAGAGGTTTCATATTGCTAATAGCAGCTACAATCCAGCTACCATTCTGCTTTTATTTT
ATGGTTGGGATAAGGCTGGATTATTCTGAGTCCAAGCTAGGCCCTTTTGCTAATCATGTTCATACCTCTT
ATCTTCCTCCCACAGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCA
CCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCA
CTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAACT
GGGGGATATTATGAAGGGCCTTGAGCATCTGGATTCTGCCTAATAAAAAACATTTATTTTCATTGCAA

2. Write that why you selected that

gene.
Because alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide
chains in adult hemoglobin, Hb A. The normal adult hemoglobin tetramer consists of two alpha
chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain
causes beta-zero-thalassemia. Reduced amounts of detectable beta globin causes beta-plus-
thalassemia. The order of the genes in the beta-globin cluster is 5'-epsilon -- gamma-G --
gamma-A -- delta -- beta--3'.

3. Find the seqaunce of its 5 orthologs. Perform MSA and also make
phylogenetic tree. (Multiple sequence alignment using MEGA tool only.
(Note that any vertion of MEGA tool can be used).

Macaca mulatta
>NC_041767.1:63067679-63069138 Macaca mulatta isolate AG07107 chromosome 14,
Mmul_10, whole genome shotgun sequence
ACAGACACCATGGTGCATCTGACTCCTGAGGAGAAGAATGCCGTCACCACCCTGTGGGGCAAGGTGAACG
TGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGTTGGTATCAAGGTTACAAGACATGCTTAAGGAGGCAAA
TAGAAGCTGGGCATGTGGAGACAGAGAAGACTCTTGGGTTTCTGATAGGCACTGACTCTCTCTGCCTATT
GGGCTATTTTCCCACCCTTAGGCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGG
GATCTGTCCTCTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTTGGTG
CCTTTAGTGATGGCCTGAATCACCTGGACAACCTCAAGGGTACCTTTGCCCAGCTCAGTGAGCTGCACTG
TGACAAGCTGCATGTGGATCCTGAGAACTTCAAGGTGAGTCTATGGGACCCTTGATGTTTTCTTTCCCCT
TCTTTTCTATGATTAACTTCATGTCATAGGAAGAGGATAAGTATCAGGGTACAGTTTAGAATGGGGAAGA
GACAAATGATTGAATCAGTGTGGAACTCTCAGGATCATTTAGTGTCTTTTATTTGCTGTTTATAACAATT
GTTTTCTTTTGTGTTTAATTCCTGCTTTCTTTTTTTTCTTCTCCGCAATTTTTATTATTATACTTAATGT
CTTAATGCTGTGTATAACAAAAGGAAATATCTTTGAGATACATTAAGTAACTTAAAAAAAACAGCTTACA
CGGTCTGCCTAGTACACTACTATTTGGAATATATGTGTGGTTATTTGCATATTCATAATCTACCTACTTT
ATTTTCTTTCTTTTTAATTAATACATAATCATTATACATATTTATTGGTTAAAGCATAATGTTTTAATAT
GTATACACATTGCATATTGACCAAATCAGGGTAATTTTGCTTTTGTAATTTTTTAAAATGCTTCTTGCTT
TAATATACTTTTTTGTTTATTTTATTTCTAATACTTCCCCTAATCTCTTTCTTTCAAGGCAATAAGCATA
CAATGTATCATGCTTCTTTGCACCATTCTAAAGAATAACAGTGATAATTTCTGGGTTAAGGCAATAGCAA
TATTTCTGCATTTAAGTAGAATATTTCTGCATATAAATTGTAACTGATGTAAGAGGTTTCATATTGCTAA
TAACAGCTATAATCCAGTGATCATTTTGCTTTTATTTTATGGTTGAGATAAGGCTGGATTATTCTGAGTC
CAAGCTAGGCCCTTTTGCTAATCATGTTCATATCTCTTGTCTCCCTCCCACAGCTCCTGGGCAACGTGCT
GGTGTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCGCAAGTGCAGGCTGCCTATCAGAAAGTG
GTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTACCACTAAGCTCACTTTCTTGCTGTC

Pongo abelii
>NC_036914.1:61910249-61911895 Pongo abelii isolate Susie chromosome 11,
Susie_PABv2, whole genome shotgun sequence
ACATTTGCTTCTGACACAATTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGA
GGAGAAGTCTGCTGTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGC
AGGTTGGTATCAAGGTTACAAGACAGGCTTAAGGAGACAAATAGAAGCTGGGCATGTGGAGACAGAGAAG
ACTCTTGGGTTTCTGATAGGCCCTGACTCTCTCTGCCTATTGGGCTATTTTCCCACCCTTAGGCTGCTGG
TGGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGATGCTGTTATGGG
CAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTGGAC
AACCTCAAGGGCACCTTTGCCAAGCTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACT
TCAGGGTGAGTCTATGGTACCATTGATGTTTTCTTTCCCCTTCTTTTCTATAGTTAAGTTCATGTCATAG
GAAGGGGATAAGTATCAGGGTACAGTTTAGAATGGGAAACTGACGAATGATTGCATCAGTGTGGAACTCT
CAGGATCATTTTAGTTTCTTTTATTTGCTGTTCATAACAATTGTTTTCTTTTGTTTAATTCTTGCTTTCT
TTTTTTTCTTCTCTGCAATTTTTACTATTATCCTTAATGCCTTAACGTTGTGTATAACAAAAGGAAATAT
CTCTGAGATACATTAAGTAACTTAAAAAAAAAGTTTACACAGTCTGCCTAGTATAATGTACTGCCAAATA
GTACATTACTATTTGGAATATATGTGTGCTTATTTGCATATTCATAATCTACCTACTTTATTTTCTTTTA
TTTTTAATTGATACATAATCATTATACATATATATGGGTTAAAGCATAATGTTTCAATATGTGTACACAT
ATTGACCAAATCAGGGTAATTTTGCATTTGTAATTTTAAAAAATGCTTTCTTGTTTTAATATACTTTTTT
GTTTATTTTATTTCTAATACTTTCCCTAATCTCTTTCTTTCAGGGCAATAATCATACAATGTATCATGCC
TCTTTGCACCATTCTAAAGAATAACAGTGATAATTTCTGGGTTAAGGCAATAGCAATATTTCTGCATATA
AATATTTCTGCATATAAATATAATATTTCTGCCTATAAATTGTAACTGACGTAAGAGGTTTCATATTGCT
AATAGCAGCTACAATCCAGCTATCATTCTGCTTTTATTTTATGGTTGGGATAAGGCTGGATTATTGTGAG
TCCAAGCTAGGCCCTTTTGCTAATCATGTTCATACCTCTTGTCTCCCTCCCACAGCTCCTGGGCAATGTG
CTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCGCAAGTGCAGGCTGCCTATCAGAAAG
TGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTACCACTAAGCTCGCTTTCTTGCTGTCCAATTTGT
ATTATAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAACTGGGGGATATTATGAAGGGCCTTGAGCATCT
GGATTCTGCCTAATAAAAAACATTTATTTTCATTGCA

Ursus maritimus
>NW_007925017.1:935063-936462 Ursus maritimus isolate Baiyulong unplaced
genomic scaffold, UrsMar_1.0 scaffold240, whole genome shotgun sequence
AAGATCAGCGCTGGGCATAAAAGGAAGAGCAGGGCCAGCTGCTGCTTATACTTGCTTCTGACACAACCGT
GTTCACTAGCAACCACAAAGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGTCTCTCGTCACCGGCC
TGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGTACCAAACCGGAAGGCAAGG
CTGGCTTACGGAGAGTGAATGGAGGCTGGGCAGGTGGAAATGCTCAGTCGCCTGGGCTTCTGACAGGCTC
TGACTCCTCTGTGCTCTGTGCTGTTTTCACCCCTCAGGCTTCTGGTTGTCTACCCCTGGACTCAGAGGTT
CTTTGACTCCTTTGGGGACCTGTCCTCTGCTGATGCTATTATGAACAACCCCAAGGTCAAGGCCCATGGC
AAGAAGGTGCTGAACTCCTTTAGTGATGGCCTGAAGAATCTGGACAACCTCAAGGGCACCTTTGCTAAGC
TGAGCGAGCTGCACTGTGACAAGCTGCACGTGGATCCCGAGAACTTCAAGGTGAGTCTGTGGGACCCTCA
GTGTTCTCCTTTTTCTTTTTTTAAGATCAAGTTCATGGTGTCCGGAAAGGGTATGAACCTTGGGTGCTGT
TTAGAGTGGAAAGGAGGTATTCCAAAGCATCAGTATGGACTCCTCAGGACTGTGTGTGTGTGTGTTTTTT
TTCTTTTACCCTCTTGCTCACAGTTGTTTCCTCTTGTTCTCTTTTTGTTTCCCGCAAATTATTTTCTTTA
TTGTTTTGATTAAACTTTTGAGTGTTTGAAAAGTGCTTTTAATCCATTCAAAATCATTTTTTTTCCTAAT
ACTGTTTTCCCTTGTCTTTTCCTTTGAAAGCAAAGAGGATAAAATCTGATTGCTTCTTTGCCTAGTTGGA
AAGAATAACCATGGTAACTTAAGTTCTGGATTAAGGCAGGAGGAAAGAAACATTTGTAAATGTAAAATCA
GTCTGATATGTTTGGAGTCACAAGGGTAGTAACATCTGAGATCCAGCTATCTTTCTGCTTATATTCTAGG
GGCATAGCTTGGGGCTGTGGATGGGGCTGGAATACGTTGAGTCTTGGCTGGGTCTGTCTGCTAATCACGC
ACATGTCTCTTGTCTCTTCCCCGCAGCTCCTGGGCAACGTGCTGGTGTGTGTGCTGGCTCACCACTTTGG
CAAAGAGTTCACCCCTCAGGTGCAGGCTGCCTATCAGAAGGTGGTGGCTGGTGTGGCCAATGCCCTGGCC
CACAAGTACCACTGAGCTCCCTTCCTGCTTTCCAGGAAGAGTCCCCTGGTCCCTAGAACCCAACACCTGA
ACTTGGGGAAATTGTGAAGTGCTTTGAGCACCTGGCTCCTGCCTAATAAAGAACACTTCTTTTCATTGCA

Chinchilla lanigera
>NW_004955414.1:c21189363-21188012 Chinchilla lanigera isolate Chin_1 unplaced
genomic scaffold, ChiLan1.0 scaffold00013, whole genome shotgun sequence
CAGGACCAACTGCTGCTTACGCTTGCTTCTGACACAGCTGTGTTTACTAGCAACCCAGAAACAGACACTA
TGGTGCACCTGACTGGTGAGGAGAAGGCTGCCGTGGCCGGCCTGTGGGAGAAAGTGAACGTGGAAGAAAT
TGGTGGTGAGGCCCTGGGCAGGTTGGTATCAGGTTATAAGATGCTTTGCGAGGCTAATGGAAGCTGGGTA
ATTGGAAACAGAGAGGTTTCCTGGGTTTCTGACAGGCGCTGATGTTCTCTGTCCCTTGGTCTGCTCTCAC
TCCTCAGGCTGCTGGTTGTCTACCCATGGACCCAGAGGTTCTTTGAGACTTTTGGGGACCTGTCCTCTGC
TTCTGCTATTATGGGCAACCCTAAAGTGAAGGCTCATGGTGCGAAGGTGCTGAACTCCTTCAGTGAGGGC
CTGCACCACCTGGACAACCTCAAGGGCACCTTCGCTAAACTGAGTGAGCTGCACTGTGACAAACTGCACG
TGGATCCTGAGAACTTCCGGGTGAGTCTGTGGGACTGGGCTCCTGTGACATCCTTCCTCTTTCTTTCTAC
AGCCACGTTCTCACCATGAGAAAAGGGAGTTAGGGTTAGGGACTAGTTTAGGATGAGAATGGTGTGTCTG
TTTGTATCCCCGTGGACCTCCGAAGATCATTTATTACACTTTATTCTGTGCTCAGTACTATTCTTTCTTT
CCTTTCCTTCTTGTTTTCCTCTTTTTTCTGTAATTTTGTCTCTTTTTTACTAAATGTTTTATTTTAAACC
AATTTTTCTTCTATTCCTCTGTTTTTGTTTGTCTTCTTTCAAACACTTTCTCTAGTTTCTTTCTAGACAA
AGTGGATAAAATGTACCATGTTTCTCCACCCAGGTTTGAATGGTAACTGTGACATTATATTTTCTGGGTT
AAGGCATTAGGGACAAATATTTCTGCCTATAAATTCTGGCTATCTTATCAGTGGTAGCAGCTATCCAGCT
ATTGTTTCATTACCAGTTTTTGGTTACAGGAAAAGCACAGGTTGAGATGAGTTTGGAATACTCTGTGTTT
AACTAGGCATGTTCGTGTCTCTCATTTCTTCCCCACAGCTCCTGGGTAACATGATTGTCATCGCTCTGGC
CCATCACTTCGGCCCGGAATTCAACCCTGCTGTGCATGCTGCCTTCCAGAAGGTCGTGGCTGGTGTGGCT
AATGCCCTGGCTCACAAGTACCACTGAGCGTTCTTTTCTGCCCTCCACTTCTTGAAGAAGGTCTCTCGGT
CCCCTGAGAACAATTACTAAATCCAGGGGAAATTATGGAGTTCCTTGAACATCTGGCTTCTGCCTAATAA
AGAACATTTATCTTCACTGCAA

Nomascus leucogenys
>NC_044395.1:c66160039-66158294 Nomascus leucogenys isolate Asia chromosome
15, Asia_NLE_v1, whole genome shotgun sequence
GGCTGTCATCACTTAGACCTCACCCTGTGGAGCCACACCCTAGGGTTGGCCAATCTACTCCCAGGAGCAG
GGAGGGCAGGAGCCAGGGCTGGGCATAAAAGTCAGGGCAGAGCCATCTATTGCTTACACTTGCTTCTGAC
GCAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCCGCCGT
TACTGCCCTGTGGGGCAAGGTGAAGGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGTTGGTATCAAGG
TTACAAGACAGGCTTAAGGAGGCAAATAGAAACTGTGCATGTGGAGACAGAGAAGGCTCTTGGGTTTCTG
ATAGGCACTGACTCTCTCTGCCTATTGGGCTATTTTCCCACCCTTAGGCTGCTGGTGGTCTACCCTTGGA
CCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAA
GGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACC
TTTGCCCAGCTGAGTGAGCTACACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGGTGAGTCTAT
GGGACCCTTGATGTTTTCTTTCCCCTTCTTTTCTATGGTTAAGTTCATGTCATAGGAAGGGGATAAGTAT
CAGGGTACAGTTTAGAATGGGAAAGAGACGAATGATTGCATCAGTGTGGAACTCTCAGGATCATTTAGTT
TCTTTTATTTGCTGTTCATAACAATTGTTTTCTTTTGTTTAATTCTTGCTTTTTTTTCTTCTCTGCAATT
TTTACTATTATACTTAATGCCTTAACGTTGTGTATAACAAAAGGAAATATCTCTGAGATACATTAAGTAA
CTTAAAAAAAGTTTACGCAGTCTGCCTAGTACATTACTATTTGGAATATATGTGTGCTTATTTGCATATT
CATAATCTACCTACTTTATTTTCTTTTATTTTTAATTGATACATAGTCATTATACATATTTATGGGTTAA
AGCATAATGTTTTAATATGTGTACACATATTGACCAAATCAGGGTAATTTTGCATTTGTAATTTTAAAAA
ATGCTTTCTTGTTTTAATATACTTTTTTGTTTATTTTATTTCTAATACATTCCCTAATCTCTTTATTTCA
GGGCAATAATCATATAATGTATCATGCCTCTTTGCACCATTCTAAAGAATAATTGTGATAATTTCTGGGT
TAAGGCAATAGCAATATTTCTGCATATAAATATTTCTGCATATAAATATAATATTTCTGCATATAAATTG
TAACTGATGTAAGAGGTTTCATATTGCTAATAGCAGCTACAATCCAGCTATCATTCTGCTTTTATTTTAT
GGTTGAGATAAGGCTGGAGTATTCTGAGTCCAAGCTTGGCCCTTTTGCTAATCATGTTCATACCTCTTGT
CTCCCTCCCACAGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACC
CCGCAAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTACCACT
AAGCTTGCTTTCTTGCTGTCCAGTTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAACTGG
GGGATATTATGAAGGGCCTTGAGCATCTGGATTCTGCCTAATAAAAAACATTTATTTTCATTGCAA

using MEGA tool

 4. Find the protein secondary structure of candidate gene sequence
and its orthologs sequences (after translation) using GOR4 and
discuss what difference you found in the secondary structure of
these sequence.

HBB hemoglobin subunit beta [ Homo sapiens (human) ]

>NC_000011.10:c5227071-5225464 Homo sapiens chromosome 11, GRCh38.p13 Primary

Assembly
ACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGA
GGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGC
AGGTTGGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAAACTGGGCATGTGGAGACAGAGAAG
ACTCTTGGGTTTCTGATAGGCACTGACTCTCTCTGCCTATTGGTCTATTTTCCCACCCTTAGGCTGCTGG
TGGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGATGCTGTTATGGG
CAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTGGAC
AACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACT
TCAGGGTGAGTCTATGGGACGCTTGATGTTTTCTTTCCCCTTCTTTTCTATGGTTAAGTTCATGTCATAG
GAAGGGGATAAGTAACAGGGTACAGTTTAGAATGGGAAACAGACGAATGATTGCATCAGTGTGGAAGTCT
CAGGATCGTTTTAGTTTCTTTTATTTGCTGTTCATAACAATTGTTTTCTTTTGTTTAATTCTTGCTTTCT
TTTTTTTTCTTCTCCGCAATTTTTACTATTATACTTAATGCCTTAACATTGTGTATAACAAAAGGAAATA
TCTCTGAGATACATTAAGTAACTTAAAAAAAAACTTTACACAGTCTGCCTAGTACATTACTATTTGGAAT
ATATGTGTGCTTATTTGCATATTCATAATCTCCCTACTTTATTTTCTTTTATTTTTAATTGATACATAAT
CATTATACATATTTATGGGTTAAAGTGTAATGTTTTAATATGTGTACACATATTGACCAAATCAGGGTAA
TTTTGCATTTGTAATTTTAAAAAATGCTTTCTTCTTTTAATATACTTTTTTGTTTATCTTATTTCTAATA
CTTTCCCTAATCTCTTTCTTTCAGGGCAATAATGATACAATGTATCATGCCTCTTTGCACCATTCTAAAG
AATAACAGTGATAATTTCTGGGTTAAGGCAATAGCAATATCTCTGCATATAAATATTTCTGCATATAAAT
TGTAACTGATGTAAGAGGTTTCATATTGCTAATAGCAGCTACAATCCAGCTACCATTCTGCTTTTATTTT
ATGGTTGGGATAAGGCTGGATTATTCTGAGTCCAAGCTAGGCCCTTTTGCTAATCATGTTCATACCTCTT
ATCTTCCTCCCACAGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCA
CCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCA
CTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAACT
GGGGGATATTATGAAGGGCCTTGAGCATCTGGATTCTGCCTAATAAAAAACATTTATTTTCATTGCAA
Translate results
>rf 1 Untitled
TFASDTTVFTSNLKQTPWCILLRRSLPLLPCGARTWMKLVVRPWAGWYQGYKTGLRRP
IETGHVETEKTLGFLIGTDSLCLLVYFPTLRLLVVYPWTQRFFESFGDLSTPDAVMGNPK
VKAHGKKVLGAFSDGLAHLDNLKGTFATLSELHCDKLHVDPENFRVSLWDACFLSPSFL
WLSSCHRKGISNRVQFRMGNRRMIASVWKSQDRFSFFYLLFITIVFFCLILAFFFFLLRN
FYYYTCLNIVYNKRKYLDTLSNLKKNFTQSAYITIWNICVLICIFIISLLYFLLFLI
DTSLYIFMGSVMFYVYTYPNQGNFAFVILKNAFFFYTFLFILFLILSLISFFQGN
NDTMYHASLHHSKEQFLGGNSNISAYKYFCIIVTDVRGFILLIAATIQLPFCFYF
MVGIRLDYSESKLGPFANHVHTSYLPPTAPGQRAGLCAGPSLWQRIHPTSAGCLSESGGW
CGCPGPQVSLSSLSCCPISIKGSFVPVQLLNWGILRALSIWILPNKKHLFSLQ
10 20 30 40 50 60 70
| | | | | | |
TFASDTTVFTSNLKQTPWCILLRRSLPLLPCGARTWMKLVVRPWAGWYQGYKTGLRRPIETGHVETEKTL
ccccccceeeeccccccceeeeccccceeccccccceeeeeecceeeeecccccceecccccccchhhhc
GFLIGTDSLCLLVYFPTLRLLVVYPWTQRFFESFGDLSTPDAVMGNPKVKAHGKKVLGAFSDGLAHLDNL
eeeeccccceeeeccccceeeeeccccceeeecccccccccccccccccccccceeeecccchhhhhhhc
KGTFATLSELHCDKLHVDPENFRVSLWDACFLSPSFLWLSSCHRKGISNRVQFRMGNRRMIASVWKSQDR
cccchhhhhhhhccccccccceeeeeccceeeccceeeeecccccccccchhhhhcceeeeeeecccccc
FSFFYLLFITIVFFCLILAFFFFLLRNFYYYTCLNIVYNKRKYLDTLSNLKKNFTQSAYITIWNICVLIC
cchhhheeeeeeccchhhhhhhceeeccceeeceeeecccccchhhhhhhccccceeeeeeeeceeeeee
IFIISLLYFLLFLIDTSLYIFMGSVMFYVYTYPNQGNFAFVILKNAFFFYTFLFILFLILSLISFFQGNN
eeechhhhhhhhhcccceeeeccceeeeeeeccccccchhhhhhccchhhhhhhhhhhhhhhhhhccccc
DTMYHASLHHSKEQFLGGNSNISAYKYFCIIVTDVRGFILLIAATIQLPFCFYFMVGIRLDYSESKLGPF
ceeeeeeeccchhhhhccccccccccceeeeeccccchhhhhhhhccccccceeeeeecccccccccccc
ANHVHTSYLPPTAPGQRAGLCAGPSLWQRIHPTSAGCLSESGGWCGCPGPQVSLSSLSCCPISIKGSFVP
ccccccccccccccccceeecccccccceeccccceeeecccccccccccccccccccccccccccccee
VQLLNWGILRALSIWILPNKKHLFSLQ
eeeechhhhhhhhhhhccccccceeec
Sequence length : 517
GOR4 :
Alpha helix (Hh) : 99 is 19.15%
310 helix (Gg) : 0 is 0.00%

Pi helix (Ii) : 0 is 0.00%

Beta bridge (Bb) : 0 is 0.00%
Extended strand (Ee) : 144 is 27.85%
Beta turn (Tt) : 0 is 0.00%
Bend region (Ss) : 0 is 0.00%
Random coil (Cc) : 274 is 53.00%
Ambiguous states (?) : 0 is 0.00%
Other states : 0 is 0.00%
>NC_041767.1:63067679-63069138 Macaca mulatta isolate AG07107 chromosome 14,
Mmul_10, whole genome shotgun sequence
ACAGACACCATGGTGCATCTGACTCCTGAGGAGAAGAATGCCGTCACCACCCTGTGGGGCAAGGTGAACG
TGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGTTGGTATCAAGGTTACAAGACATGCTTAAGGAGGCAAA
TAGAAGCTGGGCATGTGGAGACAGAGAAGACTCTTGGGTTTCTGATAGGCACTGACTCTCTCTGCCTATT
GGGCTATTTTCCCACCCTTAGGCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGG
GATCTGTCCTCTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTTGGTG
CCTTTAGTGATGGCCTGAATCACCTGGACAACCTCAAGGGTACCTTTGCCCAGCTCAGTGAGCTGCACTG
TGACAAGCTGCATGTGGATCCTGAGAACTTCAAGGTGAGTCTATGGGACCCTTGATGTTTTCTTTCCCCT
TCTTTTCTATGATTAACTTCATGTCATAGGAAGAGGATAAGTATCAGGGTACAGTTTAGAATGGGGAAGA
GACAAATGATTGAATCAGTGTGGAACTCTCAGGATCATTTAGTGTCTTTTATTTGCTGTTTATAACAATT
GTTTTCTTTTGTGTTTAATTCCTGCTTTCTTTTTTTTCTTCTCCGCAATTTTTATTATTATACTTAATGT
CTTAATGCTGTGTATAACAAAAGGAAATATCTTTGAGATACATTAAGTAACTTAAAAAAAACAGCTTACA
CGGTCTGCCTAGTACACTACTATTTGGAATATATGTGTGGTTATTTGCATATTCATAATCTACCTACTTT
ATTTTCTTTCTTTTTAATTAATACATAATCATTATACATATTTATTGGTTAAAGCATAATGTTTTAATAT
GTATACACATTGCATATTGACCAAATCAGGGTAATTTTGCTTTTGTAATTTTTTAAAATGCTTCTTGCTT
TAATATACTTTTTTGTTTATTTTATTTCTAATACTTCCCCTAATCTCTTTCTTTCAAGGCAATAAGCATA
CAATGTATCATGCTTCTTTGCACCATTCTAAAGAATAACAGTGATAATTTCTGGGTTAAGGCAATAGCAA
TATTTCTGCATTTAAGTAGAATATTTCTGCATATAAATTGTAACTGATGTAAGAGGTTTCATATTGCTAA
TAACAGCTATAATCCAGTGATCATTTTGCTTTTATTTTATGGTTGAGATAAGGCTGGATTATTCTGAGTC
CAAGCTAGGCCCTTTTGCTAATCATGTTCATATCTCTTGTCTCCCTCCCACAGCTCCTGGGCAACGTGCT
GGTGTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCGCAAGTGCAGGCTGCCTATCAGAAAGTG
GTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTACCACTAAGCTCACTTTCTTGCTGTC
>rf 1 Untitled
TDTMVHLTPEEKNAVTTLWGKVNVDEVGGEALGRLVSRLQDMLKEANRSWACGDREDSWV
SDRHLSLPIGLFSHPAAGGLPLDPEVLVLWGSVLSCCYGQPGEGSWQESAWCL
WPESPGQPQGYLCPAQAALQAACGSELQGESMGPLMFSFPFFSMINFMSEEDKYQG
TVNGEETNDISVELSGSFSVFYLLFITIVFFCVFLLSFFSSPQFLLLYLMSCCVQ
KEISLRYIKLKKNSLHGLPSTLLFGIYVWLFAYSSTYFIFFLFNYIIIIHIYWLKHN
VLICIHIAYPNQGNFAFVIFNASCFNILFCLFYFYFPSLSFKAISIQCIMLLCTIL
KNNSDNFWVKAIAIFLHLSRIFLHINCNCKRFHIANNSYNPVIILLLFYGDKAGLFV
QARPFCSCSYLLSPSHSSWATCWCVCWPITLAKNSPRKCRLPIRKWWLVWLMPWPTSTT
KLTFLL

10 20 30 40 50 60 70
| | | | | | |
TDTMVHLTPEEKNAVTTLWGKVNVDEVGGEALGRLVSRLQDMLKEANRSWACGDREDSWVSDRHLSLPIG
ccccccccccccceeeeeccceeeccccchhhhhhhhhhhhhhhhhcceeeeecccccceeccccccccc
LFSHPAAGGLPLDPEVLVLWGSVLSCCYGQPGEGSWQESAWCLWPESPGQPQGYLCPAQAALQAACGSEL
ccccccccccccccceeeeeceeeeeeeccccccceeeccccccccccccccccccccchhhhhhccccc
QGESMGPLMFSFPFFSMINFMSEEDKYQGTVNGEETNDISVELSGSFSVFYLLFITIVFFCVFLLSFFSS
ccccccceeeeccceeeeeecccccccccccccccccceeeecccccceeeeeeeeeeeeceeeeccccc
PQFLLLYLMSCCVQKEISLRYIKLKKNSLHGLPSTLLFGIYVWLFAYSSTYFIFFLFNYIIIIHIYWLKH
ceeeeeeeccccccccccchhhhhhccccccccceeeeceeeeeeeccccceeeeecceeeeeeeeeecc
NVLICIHIAYPNQGNFAFVIFNASCFNILFCLFYFYFPSLSFKAISIQCIMLLCTILKNNSDNFWVKAIA
ceeeeeeecccccccceeeeecccccccceecceecccccccceeeeeeeeeeeeeeeccccchhhhhhh
IFLHLSRIFLHINCNCKRFHIANNSYNPVIILLLFYGDKAGLFVQARPFCSCSYLLSPSHSSWATCWCVC
hhhhhhhhhhhhcccccceeecccccccceeeeeecccccceeeeeeccccceeeeeccccceeceeeee
WPITLAKNSPRKCRLPIRKWWLVWLMPWPTSTTKLTFLL
cceeeccccccceeeeeeeeeeeeeeccccccceeeeec
Sequence length : 459
GOR4 :
Alpha helix (Hh) : 48 is 10.46%
310 helix (Gg) : 0 is 0.00%

Pi helix (Ii) : 0 is 0.00%

Beta bridge (Bb) : 0 is 0.00%
Extended strand (Ee) : 173 is 37.69%
Beta turn (Tt) : 0 is 0.00%
Bend region (Ss) : 0 is 0.00%
 Random coil (Cc) : 238 is 51.85%
Ambiguous states (?) : 0 is 0.00%
Other states : 0 is 0.00%

>NC_036914.1:61910249-61911895 Pongo abelii isolate Susie chromosome 11,

Susie_PABv2, whole genome shotgun sequence
ACATTTGCTTCTGACACAATTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGA
GGAGAAGTCTGCTGTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGC
AGGTTGGTATCAAGGTTACAAGACAGGCTTAAGGAGACAAATAGAAGCTGGGCATGTGGAGACAGAGAAG
ACTCTTGGGTTTCTGATAGGCCCTGACTCTCTCTGCCTATTGGGCTATTTTCCCACCCTTAGGCTGCTGG
TGGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGATGCTGTTATGGG
CAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTGGAC
AACCTCAAGGGCACCTTTGCCAAGCTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACT
TCAGGGTGAGTCTATGGTACCATTGATGTTTTCTTTCCCCTTCTTTTCTATAGTTAAGTTCATGTCATAG
GAAGGGGATAAGTATCAGGGTACAGTTTAGAATGGGAAACTGACGAATGATTGCATCAGTGTGGAACTCT
CAGGATCATTTTAGTTTCTTTTATTTGCTGTTCATAACAATTGTTTTCTTTTGTTTAATTCTTGCTTTCT
TTTTTTTCTTCTCTGCAATTTTTACTATTATCCTTAATGCCTTAACGTTGTGTATAACAAAAGGAAATAT
CTCTGAGATACATTAAGTAACTTAAAAAAAAAGTTTACACAGTCTGCCTAGTATAATGTACTGCCAAATA
GTACATTACTATTTGGAATATATGTGTGCTTATTTGCATATTCATAATCTACCTACTTTATTTTCTTTTA
TTTTTAATTGATACATAATCATTATACATATATATGGGTTAAAGCATAATGTTTCAATATGTGTACACAT
ATTGACCAAATCAGGGTAATTTTGCATTTGTAATTTTAAAAAATGCTTTCTTGTTTTAATATACTTTTTT
GTTTATTTTATTTCTAATACTTTCCCTAATCTCTTTCTTTCAGGGCAATAATCATACAATGTATCATGCC
TCTTTGCACCATTCTAAAGAATAACAGTGATAATTTCTGGGTTAAGGCAATAGCAATATTTCTGCATATA
AATATTTCTGCATATAAATATAATATTTCTGCCTATAAATTGTAACTGACGTAAGAGGTTTCATATTGCT
AATAGCAGCTACAATCCAGCTATCATTCTGCTTTTATTTTATGGTTGGGATAAGGCTGGATTATTGTGAG
TCCAAGCTAGGCCCTTTTGCTAATCATGTTCATACCTCTTGTCTCCCTCCCACAGCTCCTGGGCAATGTG
CTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCGCAAGTGCAGGCTGCCTATCAGAAAG
TGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTACCACTAAGCTCGCTTTCTTGCTGTCCAATTTGT
ATTATAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAACTGGGGGATATTATGAAGGGCCTTGAGCATCT
GGATTCTGCCTAATAAAAAACATTTATTTTCATTGCA
>rf 1 Untitled
TFASDTIVFTSNLKQTPWCTLLRRSLLLLPCGARTWMKLVVRPWAGWYQGYKTGLRRQ
IEAGHVETEKTLGFLIGPDSLCLLGYFPTLRLLVVYPWTQRFFESFGDLSTPDAVMGNPK
VKAHGKKVLGAFSDGLAHLDNLKGTFAKLSELHCDKLHVDPENFRVSLWYHCFLSPSFL
LSSCHRKGISIRVQFRMGNRMIASVWNSQDHFSFFYLLFITIVFFCLILAFFFFFSAI
FTIILNALTLCITKGNISEIHVTKKSLHSLPSIMYCQIVHYYLEYMCAYLHIHNLPTL
FSFIFNYIIIIHIYGLKHNVSICVHILTKSGFCICNFKKCFLVLIYFFVYFISNTFPN
LFLSGQSYNVSCLFAPFRITVIISGLRQQYFCIIFLHINIIFLPINCNRKRFHIA
NSSYNPAIILLLFYGWDKAGLLVQARPFCSCSYLLSPSHSSWAMCWSVCWPITLAKNS
PRKCRLPIRKWWLVWLMPWPTSTTKLAFLLSNLYYRFLCSLSPTTKLGDIMKGLEHLDSA
KTFIFIA

10 20 30 40 50 60 70
| | | | | | |
TFASDTIVFTSNLKQTPWCTLLRRSLLLLPCGARTWMKLVVRPWAGWYQGYKTGLRRQIEAGHVETEKTL
cccccceeeeeccccccccccccccceeeccccccceeeeeecceeeeecccccchhhhhhcchhhhhhc
GFLIGPDSLCLLGYFPTLRLLVVYPWTQRFFESFGDLSTPDAVMGNPKVKAHGKKVLGAFSDGLAHLDNL
ceeeccccceeccccccceeeeeccccceeeecccccccccccccccccccccceeeecccchhhhhhhh
KGTFAKLSELHCDKLHVDPENFRVSLWYHCFLSPSFLLSSCHRKGISIRVQFRMGNRMIASVWNSQDHFS
hchhhhhhhhccccccccccceeeeeeeeeecccccccccccccceeeeehhhhcceeeeeecccccccc
FFYLLFITIVFFCLILAFFFFFSAIFTIILNALTLCITKGNISEIHVTKKSLHSLPSIMYCQIVHYYLEY
hhhheeeeeeccccchhhhcchhhhhhhhhcccccccccccccceeecccccccccceeeeeeeeeeece
MCAYLHIHNLPTLFSFIFNYIIIIHIYGLKHNVSICVHILTKSGFCICNFKKCFLVLIYFFVYFISNTFP
eeeeeccccccccccccccceeeeeecccccceeeeeeeeecccceeccccccceeeeeeceeecccccc
NLFLSGQSYNVSCLFAPFRITVIISGLRQQYFCIIFLHINIIFLPINCNRKRFHIANSSYNPAIILLLFY
cceeccccccceeeecccceeeeeecccccceeeeeeccceeeecccccccceeecccccccceeeeeec
GWDKAGLLVQARPFCSCSYLLSPSHSSWAMCWSVCWPITLAKNSPRKCRLPIRKWWLVWLMPWPTSTTKL
cccccceeecccccccceeeeeccccceeeeeeecceeeeccccccceeeeeeeeeeeeeeccccccchh
AFLLSNLYYRFLCSLSPTTKLGDIMKGLEHLDSAKTFIFIA
hhhhhhhheeeeeeccccccchhhhhhhhhccccceeeeec
Sequence length : 531
GOR4 :
Alpha helix (Hh) : 69 is 12.99%
310 helix (Gg) : 0 is 0.00%

Pi helix (Ii) : 0 is 0.00%

Beta bridge (Bb) : 0 is 0.00%
Extended strand (Ee) : 185 is 34.84%
Beta turn (Tt) : 0 is 0.00%
Bend region (Ss) : 0 is 0.00%
Random coil (Cc) : 277 is 52.17%
Ambiguous states (?) : 0 is 0.00%
Other states : 0 is 0.00%
>NW_007925017.1:935063-936462 Ursus maritimus isolate Baiyulong unplaced
genomic scaffold, UrsMar_1.0 scaffold240, whole genome shotgun sequence
AAGATCAGCGCTGGGCATAAAAGGAAGAGCAGGGCCAGCTGCTGCTTATACTTGCTTCTGACACAACCGT
GTTCACTAGCAACCACAAAGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGTCTCTCGTCACCGGCC
TGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGTACCAAACCGGAAGGCAAGG
CTGGCTTACGGAGAGTGAATGGAGGCTGGGCAGGTGGAAATGCTCAGTCGCCTGGGCTTCTGACAGGCTC
TGACTCCTCTGTGCTCTGTGCTGTTTTCACCCCTCAGGCTTCTGGTTGTCTACCCCTGGACTCAGAGGTT
CTTTGACTCCTTTGGGGACCTGTCCTCTGCTGATGCTATTATGAACAACCCCAAGGTCAAGGCCCATGGC
AAGAAGGTGCTGAACTCCTTTAGTGATGGCCTGAAGAATCTGGACAACCTCAAGGGCACCTTTGCTAAGC
TGAGCGAGCTGCACTGTGACAAGCTGCACGTGGATCCCGAGAACTTCAAGGTGAGTCTGTGGGACCCTCA
GTGTTCTCCTTTTTCTTTTTTTAAGATCAAGTTCATGGTGTCCGGAAAGGGTATGAACCTTGGGTGCTGT
TTAGAGTGGAAAGGAGGTATTCCAAAGCATCAGTATGGACTCCTCAGGACTGTGTGTGTGTGTGTTTTTT
TTCTTTTACCCTCTTGCTCACAGTTGTTTCCTCTTGTTCTCTTTTTGTTTCCCGCAAATTATTTTCTTTA
TTGTTTTGATTAAACTTTTGAGTGTTTGAAAAGTGCTTTTAATCCATTCAAAATCATTTTTTTTCCTAAT
ACTGTTTTCCCTTGTCTTTTCCTTTGAAAGCAAAGAGGATAAAATCTGATTGCTTCTTTGCCTAGTTGGA
AAGAATAACCATGGTAACTTAAGTTCTGGATTAAGGCAGGAGGAAAGAAACATTTGTAAATGTAAAATCA
GTCTGATATGTTTGGAGTCACAAGGGTAGTAACATCTGAGATCCAGCTATCTTTCTGCTTATATTCTAGG
GGCATAGCTTGGGGCTGTGGATGGGGCTGGAATACGTTGAGTCTTGGCTGGGTCTGTCTGCTAATCACGC
ACATGTCTCTTGTCTCTTCCCCGCAGCTCCTGGGCAACGTGCTGGTGTGTGTGCTGGCTCACCACTTTGG
CAAAGAGTTCACCCCTCAGGTGCAGGCTGCCTATCAGAAGGTGGTGGCTGGTGTGGCCAATGCCCTGGCC
CACAAGTACCACTGAGCTCCCTTCCTGCTTTCCAGGAAGAGTCCCCTGGTCCCTAGAACCCAACACCTGA
ACTTGGGGAAATTGTGAAGTGCTTTGAGCACCTGGCTCCTGCCTAATAAAGAACACTTCTTTTCATTGCA

>rf 1 Untitled
KISAGHKRKSRASCCLYLLLTQPCSLATTKRHHGASDWGEVSRHRPVGQGERGSWWG
PGQVPNRKARLAYGEMEAGQVEMLSRLGFQALTPLCSVLFSPLRLLVVYPWTQRFFDS
FGDLSSADAIMNNPKVKAHGKKVLNSFSDGLKNLDNLKGTFAKLSELHCDKLHVDPENFK
VSLWDPQCSPFSFFKIKFMVSGKGMNLGCCLEWKGGIPKHQYGLLRTVCVCVFFLLPSCS
QLFPLVLFLFPANYFLYCFDTFECLKSAFNPFKIIFFPNTVFPCLFLKQRGNLIASL
PSWKEPWLKFWIKAGGKKHLMNQSDMFGVTRVVTSEIQLSFCLYSRGIAWGCGWGW
NTLSLGWVCLLITHMSLVSSPQLLGNVLVCVLAHHFGKEFTPQVQAAYQKVVAGVANALA
HKYHAPFLLSRKSPLVPRTQHLNLGKLSALSTWLLPNKEHFFSL

10 20 30 40 50 60 70
| | | | | | |
KISAGHKRKSRASCCLYLLLTQPCSLATTKRHHGASDWGEVSRHRPVGQGERGSWWGPGQVPNRKARLAY
ccccccccccccceeeeeeeccccccccccccccccccccccccccceeeeccccccccccccchhhhhh
GEMEAGQVEMLSRLGFQALTPLCSVLFSPLRLLVVYPWTQRFFDSFGDLSSADAIMNNPKVKAHGKKVLN
hhhhhhhhhhhhhhcccccccccccccccceeeeeccccceeeccccccchhhhhhcchhhhhccceeee
SFSDGLKNLDNLKGTFAKLSELHCDKLHVDPENFKVSLWDPQCSPFSFFKIKFMVSGKGMNLGCCLEWKG
cccccccchhhhcchhhhhhhhccccccccccceeeeeecccccccceeeeeeeeeecceeeeeeeeccc
GIPKHQYGLLRTVCVCVFFLLPSCSQLFPLVLFLFPANYFLYCFDTFECLKSAFNPFKIIFFPNTVFPCL
cccccceeeeceeeeeeeeccccccccccceeecccccceeeeecccccccccccccceeeccccccccc
FLKQRGNLIASLPSWKEPWLKFWIKAGGKKHLMNQSDMFGVTRVVTSEIQLSFCLYSRGIAWGCGWGWNT
ccccccceeeecccccchhhhhhhhhccccceecccccceeeeeeecccceeeeeeeeeeeeeeccceee
LSLGWVCLLITHMSLVSSPQLLGNVLVCVLAHHFGKEFTPQVQAAYQKVVAGVANALAHKYHAPFLLSRK
ccccceeeeeeccccccccccccceeeeeeecccccccchhhhhhhhhhhhhhhhhhhhhcccceeeccc
SPLVPRTQHLNLGKLSALSTWLLPNKEHFFSL
cccccchhhhccchhhhhhhhccccccceeec
Sequence length : 452
GOR4 :
Alpha helix (Hh) : 85 is 18.81%
310 helix (Gg) : 0 is 0.00%

Pi helix (Ii) : 0 is 0.00%

Beta bridge (Bb) : 0 is 0.00%
Extended strand (Ee) : 119 is 26.33%
Beta turn (Tt) : 0 is 0.00%
Bend region (Ss) : 0 is 0.00%
Random coil (Cc) : 248 is 54.87%
Ambiguous states (?) : 0 is 0.00%
 Other states : 0 is 0.00%

>NW_004955414.1:c21189363-21188012 Chinchilla lanigera isolate Chin_1 unplaced

genomic scaffold, ChiLan1.0 scaffold00013, whole genome shotgun sequence
CAGGACCAACTGCTGCTTACGCTTGCTTCTGACACAGCTGTGTTTACTAGCAACCCAGAAACAGACACTA
TGGTGCACCTGACTGGTGAGGAGAAGGCTGCCGTGGCCGGCCTGTGGGAGAAAGTGAACGTGGAAGAAAT
TGGTGGTGAGGCCCTGGGCAGGTTGGTATCAGGTTATAAGATGCTTTGCGAGGCTAATGGAAGCTGGGTA
ATTGGAAACAGAGAGGTTTCCTGGGTTTCTGACAGGCGCTGATGTTCTCTGTCCCTTGGTCTGCTCTCAC
TCCTCAGGCTGCTGGTTGTCTACCCATGGACCCAGAGGTTCTTTGAGACTTTTGGGGACCTGTCCTCTGC
TTCTGCTATTATGGGCAACCCTAAAGTGAAGGCTCATGGTGCGAAGGTGCTGAACTCCTTCAGTGAGGGC
CTGCACCACCTGGACAACCTCAAGGGCACCTTCGCTAAACTGAGTGAGCTGCACTGTGACAAACTGCACG
TGGATCCTGAGAACTTCCGGGTGAGTCTGTGGGACTGGGCTCCTGTGACATCCTTCCTCTTTCTTTCTAC
AGCCACGTTCTCACCATGAGAAAAGGGAGTTAGGGTTAGGGACTAGTTTAGGATGAGAATGGTGTGTCTG
TTTGTATCCCCGTGGACCTCCGAAGATCATTTATTACACTTTATTCTGTGCTCAGTACTATTCTTTCTTT
CCTTTCCTTCTTGTTTTCCTCTTTTTTCTGTAATTTTGTCTCTTTTTTACTAAATGTTTTATTTTAAACC
AATTTTTCTTCTATTCCTCTGTTTTTGTTTGTCTTCTTTCAAACACTTTCTCTAGTTTCTTTCTAGACAA
AGTGGATAAAATGTACCATGTTTCTCCACCCAGGTTTGAATGGTAACTGTGACATTATATTTTCTGGGTT
AAGGCATTAGGGACAAATATTTCTGCCTATAAATTCTGGCTATCTTATCAGTGGTAGCAGCTATCCAGCT
ATTGTTTCATTACCAGTTTTTGGTTACAGGAAAAGCACAGGTTGAGATGAGTTTGGAATACTCTGTGTTT
AACTAGGCATGTTCGTGTCTCTCATTTCTTCCCCACAGCTCCTGGGTAACATGATTGTCATCGCTCTGGC
CCATCACTTCGGCCCGGAATTCAACCCTGCTGTGCATGCTGCCTTCCAGAAGGTCGTGGCTGGTGTGGCT
AATGCCCTGGCTCACAAGTACCACTGAGCGTTCTTTTCTGCCCTCCACTTCTTGAAGAAGGTCTCTCGGT
CCCCTGAGAACAATTACTAAATCCAGGGGAAATTATGGAGTTCCTTGAACATCTGGCTTCTGCCTAATAA
AGAACATTTATCTTCACTGCAA
>rf 1 Untitled
QDQLLLTLASDTAVFTSNPETDTMVHLTGEEKAAVAGLWEKVNVEEIGGEALGRLVSGYK
MLCEANGSWVIGNREVSWVSDRRCSLSLGLLSLLRLLVVYPWTQRFFETFGDLSSASAI
MGNPKVKAHGAKVLNSFSEGLHHLDNLKGTFAKLSELHCDKLHVDPENFRVSLWDWAPVT
SFLFLSTATFSPEKGVRVRDFRMRMVCLFVSPWTSEDHLLHFILCSVLFFLSFPSCFP
LFSVILSLFYMFYFKPIFLLFLCFCLSSFKHFLFLSRQSGNVPCFSTQVMVTVTLY
FLGGIRDKYFCLILAILSVVAAIQLLFHYQFLVTGKAQVEMSLEYSVFNACSCLSFL
PHSSWVTLSSLWPITSARNSTLLCMLPSRRSWLVWLMPWLTSTTERSFLPSTSRRSLG
PLRTITKSRGNYGVPTSGFCLIKNIYLHC

10 20 30 40 50 60 70
| | | | | | |
QDQLLLTLASDTAVFTSNPETDTMVHLTGEEKAAVAGLWEKVNVEEIGGEALGRLVSGYKMLCEANGSWV
cccceeeecccceeeccccccchhhhhchhhhhhhhhhhhhhchhhhcchhhhhhhhhhhhhhhccccee
IGNREVSWVSDRRCSLSLGLLSLLRLLVVYPWTQRFFETFGDLSSASAIMGNPKVKAHGAKVLNSFSEGL
eeccceeeeccccchhhhhhhhhhheeeeccccchhhhcccccchhhhhhccchhhhccceeeccccccc
HHLDNLKGTFAKLSELHCDKLHVDPENFRVSLWDWAPVTSFLFLSTATFSPEKGVRVRDFRMRMVCLFVS
chhhhhhchhhhhhhhccccccccccceeeeeccccceeeeeeccccccccccceeeeeeeeeeeeeeec
PWTSEDHLLHFILCSVLFFLSFPSCFPLFSVILSLFYMFYFKPIFLLFLCFCLSSFKHFLFLSRQSGNVP
ccccccceeeeeeceeeeecccccccccceeehhhhhhhcccceeeeeeccccccccceeeeeccccccc
CFSTQVMVTVTLYFLGGIRDKYFCLILAILSVVAAIQLLFHYQFLVTGKAQVEMSLEYSVFNACSCLSFL
eeeeccceeeeeeeccccccchhhhhhhhhhhhhhhhhhhhhhhhhccchhhhhhheeeeeccceeeeec
PHSSWVTLSSLWPITSARNSTLLCMLPSRRSWLVWLMPWLTSTTERSFLPSTSRRSLGPLRTITKSRGNY
cccceeeeccccceecccccceeeeecccceeeeeeeccccccccccccccccccccccceeeeeccccc
GVPTSGFCLIKNIYLHC
ccccccceeeeceeecc
Sequence length : 437
GOR4 :
Alpha helix (Hh) : 116 is 26.54%
310 helix (Gg) : 0 is 0.00%

Pi helix (Ii) : 0 is 0.00%

Beta bridge (Bb) : 0 is 0.00%
Extended strand (Ee) : 124 is 28.38%
Beta turn (Tt) : 0 is 0.00%
Bend region (Ss) : 0 is 0.00%
Random coil (Cc) : 197 is 45.08%
Ambiguous states (?) : 0 is 0.00%
Other states : 0 is 0.00%
>NC_044395.1:c66160039-66158294 Nomascus leucogenys isolate Asia chromosome
15, Asia_NLE_v1, whole genome shotgun sequence
GGCTGTCATCACTTAGACCTCACCCTGTGGAGCCACACCCTAGGGTTGGCCAATCTACTCCCAGGAGCAG
GGAGGGCAGGAGCCAGGGCTGGGCATAAAAGTCAGGGCAGAGCCATCTATTGCTTACACTTGCTTCTGAC
GCAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCCGCCGT
TACTGCCCTGTGGGGCAAGGTGAAGGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGTTGGTATCAAGG
TTACAAGACAGGCTTAAGGAGGCAAATAGAAACTGTGCATGTGGAGACAGAGAAGGCTCTTGGGTTTCTG
ATAGGCACTGACTCTCTCTGCCTATTGGGCTATTTTCCCACCCTTAGGCTGCTGGTGGTCTACCCTTGGA
CCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAA
GGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACC
TTTGCCCAGCTGAGTGAGCTACACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGGTGAGTCTAT
GGGACCCTTGATGTTTTCTTTCCCCTTCTTTTCTATGGTTAAGTTCATGTCATAGGAAGGGGATAAGTAT
CAGGGTACAGTTTAGAATGGGAAAGAGACGAATGATTGCATCAGTGTGGAACTCTCAGGATCATTTAGTT
TCTTTTATTTGCTGTTCATAACAATTGTTTTCTTTTGTTTAATTCTTGCTTTTTTTTCTTCTCTGCAATT
TTTACTATTATACTTAATGCCTTAACGTTGTGTATAACAAAAGGAAATATCTCTGAGATACATTAAGTAA
CTTAAAAAAAGTTTACGCAGTCTGCCTAGTACATTACTATTTGGAATATATGTGTGCTTATTTGCATATT
CATAATCTACCTACTTTATTTTCTTTTATTTTTAATTGATACATAGTCATTATACATATTTATGGGTTAA
AGCATAATGTTTTAATATGTGTACACATATTGACCAAATCAGGGTAATTTTGCATTTGTAATTTTAAAAA
ATGCTTTCTTGTTTTAATATACTTTTTTGTTTATTTTATTTCTAATACATTCCCTAATCTCTTTATTTCA
GGGCAATAATCATATAATGTATCATGCCTCTTTGCACCATTCTAAAGAATAATTGTGATAATTTCTGGGT
TAAGGCAATAGCAATATTTCTGCATATAAATATTTCTGCATATAAATATAATATTTCTGCATATAAATTG
TAACTGATGTAAGAGGTTTCATATTGCTAATAGCAGCTACAATCCAGCTATCATTCTGCTTTTATTTTAT
GGTTGAGATAAGGCTGGAGTATTCTGAGTCCAAGCTTGGCCCTTTTGCTAATCATGTTCATACCTCTTGT
CTCCCTCCCACAGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACC
CCGCAAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTACCACT
AAGCTTGCTTTCTTGCTGTCCAGTTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAACTGG
GGGATATTATGAAGGGCCTTGAGCATCTGGATTCTGCCTAATAAAAAACATTTATTTTCATTGCAA
>rf 1 Untitled
GCHHLDLTLWSHTLGLANLLPGAGRAGARAGHKSQGRAIYCLHLLLTQLCSLATSNRHHG
APDSGEVRRYCPVGQGEGGSWWGPGQVGIKVTRQAGGKKLCMWRQRRLLGFAL
TLSAYWAIFPPLGCWWSTLGPRGSLSPLGICPLLMLLWATLRRLMARKCSVPLVMAWLT
WTTSRAPLPSVSYTVTSCTWILRTSGVYGTLDVFFPLLFYGVHVIGRGVSGYSLEW
ERDELHQCGTLRIIFLLFAVHNNCFLLFNSCFFFFSAIFTIILNALTLCITKGNISEI
HVTKKFTQSAYITIWNICVLICIFIIYLLYFLLFLIDTSLYIFMGSIMFYVYTY
PNQGNFAFVILKNAFLFYTFLFILFLIHSLISLFQGNNHIMYHASLHHSKELFLG
GNSNISAYKYFCIIYFCIIVTDVRGFILLIAATIQLSFCFYFMVEIRLEYSESKLG
PFANHVHTSCLPPTAPGQRAGLCAGPSLWQRIHPASAGCLSESGGWCGCPGPQVPLSLL
SCCPVSIKGSFVPVQLLNWGILRALSIWILPNKKHLFSLQ

10 20 30 40 50 60 70
| | | | | | |
GCHHLDLTLWSHTLGLANLLPGAGRAGARAGHKSQGRAIYCLHLLLTQLCSLATSNRHHGAPDSGEVRRY
cccccccchhhhhhhhhhcccccchhhhhhccccccceeeeeeecccccccccccccccccccccceeee
CPVGQGEGGSWWGPGQVGIKVTRQAGGKKLCMWRQRRLLGFALTLSAYWAIFPPLGCWWSTLGPRGSLSP
eeeeeeeccccccccccceeeeeccccchhhhhhhhhhhhhhhhhhhhhhhhcccccceecccccccccc
LGICPLLMLLWATLRRLMARKCSVPLVMAWLTWTTSRAPLPSVSYTVTSCTWILRTSGVYGTLDVFFPLL
ccccchhhhhhhhhhhhhhhccceeeeeeccccccccccccceeeeecceeeeeeecccccccccccccc
FYGVHVIGRGVSGYSLEWERDELHQCGTLRIIFLLFAVHNNCFLLFNSCFFFFSAIFTIILNALTLCITK
ccceeeeecccccchhhhchhhhhcccceeeeeeeeeecccceeeccccceeccchhhhhhccccccccc
GNISEIHVTKKFTQSAYITIWNICVLICIFIIYLLYFLLFLIDTSLYIFMGSIMFYVYTYPNQGNFAFVI
ccccceeecccccceeeeeeeceeeeeeechhhhhhhhhhhcccceeeeecceeeeeeeccccccchhhh
LKNAFLFYTFLFILFLIHSLISLFQGNNHIMYHASLHHSKELFLGGNSNISAYKYFCIIYFCIIVTDVRG
hhccchhhhhhhhhhhhhhhhhhcccccceeeeeeeccchhhhhccccccceeeeeceeeeeeecccccc
FILLIAATIQLSFCFYFMVEIRLEYSESKLGPFANHVHTSCLPPTAPGQRAGLCAGPSLWQRIHPASAGC
hhhhhhhhhhhhccceeeeeechhhhcccccccccccccccccccccccceeecccccccceecccccee
LSESGGWCGCPGPQVPLSLLSCCPVSIKGSFVPVQLLNWGILRALSIWILPNKKHLFSLQ
eecccccccccccccceeecccccccccccceeeeeechhhhhhhhhhhccccccceeec
Sequence length : 550
GOR4 :
Alpha helix (Hh) : 137 is 24.91%
310 helix (Gg) : 0 is 0.00%

Pi helix (Ii) : 0 is 0.00%

Beta bridge (Bb) : 0 is 0.00%
Extended strand (Ee) : 138 is 25.09%
Beta turn (Tt) : 0 is 0.00%
Bend region (Ss) : 0 is 0.00%
Random coil (Cc) : 275 is 50.00%
Ambiguous states (?) : 0 is 0.00%
Other states : 0 is 0.00%