CULTURE MEDIA EMPLOYED IN DIAGNOSTIC MICROBIOLOGY (1-50)
NAMES CLASSIFICATION
1. Amie’s Medium
2. Cary and Blair Med
3. Alkaline Peptone Water
4. Blood Agar
5. Chocolate Agar
6. Dextrose Ascitic Fluid Agar
7. Gram Negative Broth (GN)
8. Anaerobic Blood Agar
9. AnaerobicPhenylethylalcoholAgar(PEA)
10. AnaerobicKanamycinVancomycinBA
FUNCTION/ S
To maintain the viability of the
Transport organisms from source to the
laboratory
To maintain the viability of the
Transport organisms from source to the
laboratory
To increase the no. of pathogenic
Enrichment
Vibrio in the specimen
Enriched with unheated blood;
Enriched and differential Differentiates organism thru
hemolytic patterns
Enriched with heated blood. For the
Enriched cultivation of most organisms esp.
Haemophilus and Neisseria spp.
Enriched To isolate organisms in CSF
To increase the no. of pathogens in
Enrichment broth
stools
Reducing or anaerobic For isolation of all anaerobes
For isolation of obligate Gr+ and Gr-
anaerobes. It inhibits growth of
Reducing or anaerobic
enteric bacilli and swarming of
Proteus
For isolation of Gr- anaerobes esp.
Reducing or anaerobic Bacteroides Prevotella and some
Fusobacterium
 CULTURE MEDIA EMPLOYED IN DIAGNOSTIC MICROBIOLOGY (1-50)

For isolation of Gr- anaerobes esp.

Bacteriodes, Prevotella and some
11. AnaerobicParomomycinVancomycinLakedBA Reducing or anaerobic
Fusobacterium Detection of Pigment
production of Prevotella
Selective for Bacteriodes sps. Used to
12. Bacteriodes Bile Esculin (BBE) Selective and differential differentiate bile resistant from bile
sensitive Bacteriodes sps.
Selective for Gr neg enteric bacilli. It
13. Eosin Methylene Blue Agar (EMB) differentiates lactose from non-
Selective and differential
14. Hektoen Enteric Agar (HEA) lactose fermenting Gr neg enteric
bacilli.
Selective for Group D Streptococcus
15. Bile Esculin Medium (BEM) Selective
& Enterococcus
Selective for the isolation of
16. Bismuth Sulfite Agar (BSA) or Wilson Blair Agar Selective
Salmonella typho
Selective for the isolation of
17. Bordet Gengou Agar or Potato Blood Glycerol Agar Selective
Bordetella pertussis
18. Brucella Blood Agar
19. Biphasic media of Brucella Broth & Agar in Castaneda Selective For the isolation of Brucella spp.
bottle or septi- check
For the isolation of Francisella
20. Blood CystineGlucose Agar with thiamine Selective
tularensis
For the isolation of Legionela
21. Buffered Charcoal Yeast Extract Agar (BCYE) Selective
pneumophila
For the isolation of Yersinia
22. Cefsulodin Irgasan Novobiocin Agar (CIN) Selective
enterocolitica
23. Campy Blood Agar
For the isolation of Campylobacter
24. Campylobacter Thioglycollate Broth Selective
spp.
25. Cefoperazone, Vancomyin, Amphotericin Medium
 CULTURE MEDIA EMPLOYED IN DIAGNOSTIC MICROBIOLOGY (1-50)

26. Chapman- Stone Agar Selective For the isolation of Staphylococci

For the isolation of Staphylococci and
27. Columbia Colistin Nalidixic Agar (CAN) Selective
Streptococci from mixed specimens
28. Cooked meat or chopped meat medium Selective For the isolation of Anaerobes
For the isolation of Clostridium
29. Cycloserine Cefoxilitin Fructose Agar (CCFA) Selective
difficile
For the isolation and enumeration of
30. Cystine Lactose electrolyte Deficient Agar (CLED) Selective
bacteria in urine
31. Cystine Tellurite Blood Agar For the isolation of Corynebacterium
Selective
32. Loeffler’s coagulated serum slants diphtheriae
For the isolation of Clostridium
33. Clostrisel Agar Selective
perfringens
34. Dieudonne’s Medium Selective For the isolation of Vibrio spp.
For the isolation of Mycoplasma &
35. “E” Agar Selective
Ureaplasma spp.
For the isolation of Mycoplasma &
36. Edward Hayflick Agar Selective
Ureaplasma spp.
37. Ellinghausen, McCullough, Johnson, & Harris
For the isolation of Leptospira
(EMJH) Medium Selective
interrogans
38. Flectcher’s semi- solid medium
For the isolation of Haemophilus
39. FetalBovineSerumAgar with Vancomycin Selective
ducreyi
For the isolation of Gardnerella
40. HumanBloodBilayerTween (HBT) Agar Selective
vaginalis
41. Modified Kelly’s medium or Barbour- Stoenner- Kelly
Selective For the isolation of Borrelia spp.
(BSK-II)
For the isolation of Mycobacterium
42. Lowenstein Jensen Agar Selective
spp.
 CULTURE MEDIA EMPLOYED IN DIAGNOSTIC MICROBIOLOGY (1-50)
43. Lombard- Dowell Agar
44. Lowenstein Jensen (LJ) with antibiotics
45. Crystine trypticase Agar (CTA)
46. Lysine Iron Agar (LIA)
47. DNA Agar
48. Egg Yolk Agar (EYA)
49. 12% Gelatin medium
50. Brain Heart Infusion (BHI) broth or agar
Selective For isolation of anaerobic organisms
For isolation of Mycobacterium
Selective
species
For identification of Neisseria spp
Biochemical characteristic
based on their ability to utilize
medium
carbohydrate
Used to identify Gram neg Enteric
bacilli based on their ability to
Biochemical characteristic
decarboxylate or deaminate lysine
medium
and to produce hydrogen sulfide or
not
To identify Staphylococcus aureus
Lab diagnostic test medium based on its ability to produce
DNASE enzyme
To identify Clostridium perfringens
Lab diagnostic test medium based on its ability to produce
lecithinase
For the identification of organisms
Lab diagnostic test medium
which liquefy or hydrolyze gelatin
Basic medium for isolation of
microorganisms and can be used for
Basic or gen purpose medium
preparing pure culture for antibiotic
susceptibility test