Stain Purpose Fixation Result Section

 Apple green
Direct immunoflourescence is Liquid fixative flourescein is used as Frozen section
usually performed on thin Michel’s fixative fluoro-chrome
cryostat sections of fresh,  Orange-red
unfixed materials. In this fluorescein with
technique, the tissue us rhodamine conjugate
reacted directky with a
flourescein-conjugated
antibody specific for the
Direct Immunoflourescence material being sought within
the tissue

The indirect Formalin The results are subjective Frozen

immunoflourescence and indirect
techniques is mainly used for immunofluorescence
the detection of autoantibodies cannot be used reliably to
in the patient’s serum, monitor disease severity
including the anti-nuclear and its treatment. 
antibody, anti-mitochondrial
antibody and liver-kidney
microsomal antibody/
Indirect Immunoflourescence

Staining of acetone-fixed Acetone, or formalin Antibodies are visible Superfrost section

frozen tissues using a purified,
unconjugated primary
antibody, biotinylated
 secondary antibody and
streptavidin-horseradish
peroxidase (SAv-HRP) and
DAB detection system.

Frozen Section
Immunohistochemistry

References
Gregorios, Jocelyn and Faldas, Marc, 2017, Histopathologic Techniques
Xuan,Rachel, 2017, Retrieved from https://dermnetnz.org/topics/indirect-immunofluorescence/