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Reactions of Plant Glycolysis and Fermentation
Reactions of Plant Glycolysis and Fermentation
Electron transport
Complex I, NAD(P)H
dehydrogenases, and Complex II
feed electrons to a pool of
ubiquinones
Complex I pumps 4 H+ out of the matrix
for each e- transferred
Ubiquinone passes e- to Complex III
and, via Cytochrome c, on to
Complex IV
Both complexes pump H+ out of the
matrix
Complex IV uses O2 as the e- acceptor
to produce H2O. Poisoned by cyanide.
The alternative oxidase can also accept
e- from ubiquinone to reduce O2 to H2O,
but does not have the accompanying H+
transport. Inhibited by SHAM
ATP synthase (complex V) functions
like the chloroplast ATP synthase,
but is driven more by charge
difference (ΔE) than H+ gradient.
NADH and NADPH are oxidized as the source of e- .
NAD(P)+ reduced by Glycolysis, Krebs cycle, PSI
Figure 12.10 Transmembrane transport in plant mitochondria
Aerobic respiration
Yields approximately 60 ATP per sucrose
52 from oxidative phosphorylation via 20 molecules
NAD(P)H and 4 FADH2 molecules
8 from substrate-level phosphorylation
Represents 52% energy efficiency (vs. 4% from
glycolysis/fermentation)
Plant mitochondria
Genome is much larger than in animals and fungi
RNA splicing occurs on a limited basis
RNA editing occurs (~ C U)
RNA stability signals differ
Use the universal genetic code (unlike other organisms’
mitochondrial genomes)
Plants can decrease efficiency
Why?
Energy may not be limiting
Plasticity for use of metabolites may be
more important
Alternative oxygenase reduces O2 to
water, siphoning off excess electrons
and producing heat
Stresses that increase Reactive Oxygen
Species (ROS) activate alternative
oxygenase to prevent overreduction
Uncoupling protein relieves the H+
gradient without producing ATP
Non-proton-pumping NADH
dehydrogenases when ADP is
limiting
ADP and Pi are major regulators of
respiration
Figure 12.11 Metabolic interactions between mitochondria and
cytosol
11.11 Metabolic regulation of pyruvate dehydrogenase (PDH)
activity
Regulatory kinases and phosphatases control
Pyruvate dehydrogenase, coupling energy status
and metabolites to activity
Lipids in plants
Triacylglycerols
Storage fats and oils
High metabolic energy content
Polar glycerolipids
Membrane components
Polar glycerolipids
Primary membrane structural lipids
Glyceroglycolipids
Sugar head group
Abundant in chloroplast membranes
Glycerophospholipids
Phosphate on head group
Sphingolipids, sterols, and other lipids are also membrane
components
Plastoquinones, chlorophylls, carotenoids, tocopherols
involved in photosynthesis and other functions
Very abundant in photosynthetic tissues