EDUARDO MONDLANE UNIVERSITY

FACULTY OF ENGINEERING

MASTER'S DEGREE IN HYDROCARBON PROCESSING ENGINEERING

ANALYTICAL TECHNIQUES

ASSIGNMENT 1

AUTHOR:
BONAVENTURE, UWIRAGIYE
LECTURER:

CARVALHO MADIVATE

MAPUTO, MAY 2018

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 1. For single standard

S= Ka×Ca

Where: S=signal, ka=sensitivity of analyte, Ca=concentration of analyte

0,22
Kvitamin C= =1.29 ×10−3 ppm−1
170 ppm

1,35
Kniacin= =1.04 ×10−2 ppm−1
130 ppm

0,90
Kniacinamide= =7.50 ×10−3 ppm−1
120 ppm

1,37
Kpyridoxine= =9.13× 10−3 ppm−1
150 ppm

0,82
KThiamine= =1.37× 10−2 ppm−1
60 ppm

0,36
Kfolic acid= =2.40 ×10−2 ppm−1
15 ppm

0,29
Kriboflavin= =2.90 ×10−2 ppm−1
10 ppm

So the concentrations in the sample are calculated as follows

0.87
C vitC= =674 ppm
1.29 ×10−3 ppm−1

0.00
C niacin= =0 ppm
1.04 ×10−2 ppm −1

2
 1.40
C niacinamide= =187 ppm
7.5× 10−3 ppm−1

0.22
C pryidoxine= =24.10 ppm
9.13 ×10−3 ppm−1

0.19
C thiamine= =13.90 ppm
1.37× 10−2 ppm−1

0.11
C folic acid= =4.58 ppm
2.40 ×10−2 ppm−1

0.44
C riboflavin= =15.20 ppm
2.9 × 10−2 ppm−1

To prepare the tablet for analysis, we dissolved it in 100 mL of solvent

(10 mL of 1% v/v NH3 in dimethyl sulfoxide and 90 mL of 2% acetic
acid); thus, we multiply each concentration by 0.100 L to arrive at the
mass of each analyte

m vitamin C=674 ppm ×0.100 l=67.4 mg

m niacin=0.00 ppm × 0.100l=0 mg

m niacinamide=187 ppm× 0.100 l=18.70 mg

m pyridoxine=24.10 ppm× 0.100l=2.41mg

m thiamine=13.90 ppm ×0.100 l=1.39 mg

m folic acid=4.58 ppm×0.100 l=0.46 mg

m riboflavin=15.20 ppm×0.100 l=1.52 mg

2. Let mA represent the milligrams of vitamin A in a 10.067 g portion of

cereal. For different amount of cereal in the standard addition,

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 10.093 g, the cereal’s contribution of vitamin A to the standard
addition is
10.093 g
mA
10.067 g

And the relation between the signal to the mass of vitamin A in the
sample and in the standard addition is
Ssample=KmA
10.093 g
Sstd add=K mA × { 10.067 g
+0.02 mg }
By solving the two equations
Ssample Sint . std
=
mA 10.093 g
mA × +0.02 mg
10.067 g

6.77 ×103 1.32 ×10 4

=
mA 10.093 g
mA × +0.02 mg
10.067 g
( 6.7875 ×103 ) mA +135.4 mg=( 1.32 ×104 ) mA
6412.5 mA=135.4 mg
mA =0.00211 mg
The vitamin A content of cereal is:
0.0211 mgVitaminA
× 100=0.211 mgVitaminA par 100 g cereal
10.067 gSample