Autoradiography Guide

The Complete
Autoradiography
Product
Guide
Hyperfilm
Kodak Film
Autoradiography
Accessories
Introduction
Amersham Pharmacia Biotech is one of the world’s leading suppliers of autoradiography
and light detection products. Using its expertise in signal generation, Amersham
Pharmacia Biotech has developed Hyperfilm™ and accessory products specifically for
use in life science applications, providing quality results and capturing the maximum
amount of information.
As well as our own well-established autoradiography range, the company is also
a worldwide distributor of Kodak™ Scientific Imaging Systems’ autoradiography and
imaging products. Amersham Pharmacia Biotech is therefore able to advise customers on
the best solution for their imaging needs from one of the world’s largest selection of
products from a single source.
In addition to this catalogue, Review No.23, Efficient Detection of Bio-molecules by
Autoradiography, Fluorography or Chemiluminesence, by R. Laskey, is also available.
Review 23 is an independently written reference, that describes in detail, the principles
of indirect and direct autoradiography.
Review 23 is available on request, please contact your
local sales office.

Contents
Life science application guide 1–2
Contents 3
Autoradiography films:
Hyperfilm MP 4–5
Hyperfilm βmax 6
Hyperfilm 3H 7
Hyperfilm ECL 8
Kodak BioMax MR 9
Kodak BioMax MS 10
Kodak BioMax Light 11
Kodak X-Omat AR (XAR) 12
Kodak X-Omat LS (XLS) 12
Autoradiography cassettes and screens:
Hyperscreen intensifying screens 13
Kodak BioMax MS intensifying screens 14
Kodak BioMax TranScreen intensifying screens 15
Hypercassette autoradiography cassettes 16
Autoradiography accessories:
Amplify fluorographic reagent 17
Darkroom lamp 18
Hypertorch 18
ECL mini camera 19
Hypercoat nuclear emulsions 20
Hyperprocessor automatic film processor 21
Liquid scintillation cocktails 22 – 23
Microscale autoradiography standards 24
Sensitize preflash unit 25
Processing hanger 26
TrackerTape 26
Other film products:
Kodak 35mm Black & White film 27
Kodak 35mm Colour Slide film 28
Kodak Duplicating film 29
Kodak micro-autoradiography & TEM products 30
3
Life science application guide
Autoradiography methods and materials for life science applications
Application Result required Label
Southern/Northern blots high speed and sensitivity 32P
Plaque/colony lifts accurate quantitation

maximum speed and sensitivity 32P
optimum resolution 33P/35S
non-radioactive, quantification ECL, Gene Images

Slot blots high speed and sensitivity 32P
accurate quantitation
maximum speed and sensitivity 32P
non-radioactive, quantification ECL, Gene Images

Dideoxy sequencing high speed 32P
optimum resolution and maximum 35S
speed for difficult sequences

good resolution and speed for 35S
routine work
Cycle sequencing optimum resolution and maximum 33P
speed for difficult sequences

good resolution and speed for 33P
routine work
Protein synthesis, lysates maximum speed 35S/14C/3H
optimum resolution 125I
Western blots maximum speed and sensitivity 125I
accurate quantification
optimum resolution 35S/14C
non-radioactive/maximum sensitivity ECL

In situ hybridization macroscale in situ optimization 32P
of parameters
Receptor studies localization at cellular level:
– intermediate resolution 33P/35S
– high resolution 33P/35S
localization subcellular level:

– intermediate resolution 125I/3H
– high resolution 125I/3H
Whole body optimum resolution 125I/3H
autoradiography high resolution 35S/14C
1
Method Film Support products
Pre-flash and screens at -70ºC Hyperfilm MP Sensitize, Hyperscreen
Screens at -70ºC BioMax MS BioMax TranScreen HE

Direct autoradiography Hyperfilm βmax/BioMax MR
Pre-flash and direct detection Hyperfilm ECL Sensitize
Screens at -70ºC BioMax MS film BioMax TranScreen HE

Direct autoradiography Hyperfilm MP/BioMax MR
Direct autoradiography BioMax MR
Direct autoradiography Hyperfilm MP
Direct autoradiography BioMax MR
Pre-flash and fluorography at -70ºC Hyperfilm MP Amplify, Sensitize

Indirect autoradiography BioMax MS BioMax TranScreen HE
Direct autoradiography Hyperfilm 3H
Screens at -70ºC BioMax MS film BioMax TranScreen HE
Direct autoradiography Hyperfilm βmax/BioMax MR
Direct autoradiogaphy Hyperfilm βmax/BioMax MR

Micro autoradiography Hypercoat emulsion LM-1

Micro autoradiography Hypercoat emulsion EM-1
Direct autoradiography Hyperfilm βmax/BioMaxMR
2
Autoradiography films
Hyperfilm MP
Product description
Hyperfilm™ MP is a multipurpose film constructed from a clear plastic base coated on both sides
with a photographic emulsion that is then protected by an anti-scratch layer. In a multi-discipline
Features laboratory, performing both blots and sequencing experiments, Hyperfilm MP is the recommended
film for high performance in all applications.
and benefits The emulsion of Hyperfilm MP is very sensitive to light making it ideal for indirect autoradiography.
Hyperfilm MP The combination of this film with fluorographic reagents or intensifying screens will give high
● Multi-purpose film for the sensitivity. Its excellent contrast and resolution also make it suitable for the direct autoradiography
high speed detection of 3H, of sequences. It may be processed using automatic processors or in tanks and trays using most
14C, 35S, 33P, 32P and 125I
common laboratory X-ray film developers and fixers. Normal safelight conditions are applicable.
● Coated with emulsion
Hyperfilm MP is also available in an envelope sealed format. Here, individual sheets of Hyperfilm
on both sides to ensure
MP are sealed in light tight envelopes giving maximum protection and ease of use in the life science
optimum sensitivity
laboratory. Each envelope is made from paper with an internal lining of low density polymer.
● High resolution results at an
In addition a single sheet of paper interleaving between the film and envelope to prevent static.
economical price
● Clear base offers high
contrast against blackened
image for easy reading
screen 1
● Designed for use in direct
autoradiography or indirect emulsion
autoradiography.The film of anti-
choice for multipurpose work scratch plastic base
layer
● Suitable for pre-flashing with emulsion
the Sensitize™ preflash unit
and in combinations with
most intensifying screens
including Hyperscreen™,
32 125
TranScreen™ and BioMax™ P/ I
MS intensifying screens radioactive emissions
light emission
Hyperfilm MP envelope
● Envelope ensures accidental FIGURE 1
exposure is limited to one Diagram of Hyperfilm MP being used with an
sheet, not a whole box intensifying screen
● Confidence when sharing a

box of film, with each sheet
in good condition
Applications
● Only individual sheets need to
● 33P, 32P and 125I Northern, Southern or Western blots
be carried to the darkroom
● 35S, 33P and 32P Sequencing
● Better long term storage
once the film box is opened, ● Fluorography of 3H, 14C and 35S
FIGURE 2
film continues to be
Cycle sequencing with 33P
protected from moisture labelled primers
4
0.0001µCi/cm2
2.0 (fluorography at
0.1µCi/cm2
-70˚C pre-flashed)
1.5
optical density
0.01µCi/cm2 0.001µCi/cm2
1.0
0.5
0.0001µCi/cm2
0
0 20 40 60 80
hours exposure
FIGURE 3
32
P direct and indirect autoradiography
at 20ºC, Hyperfilm MP
Hyperfilm MP
Code Size Quantity
RPN6H 18x24cm 25 sheets
RPN1676H 5x7in 25 sheets
RPN34H 20cmx25m 1 roll
Hyperfilm MP envelope
Code Size Quantity
RPN6J 18x24cm 50 sheets
RPN8J 35x43cm 50 sheets
RPN1677J 8x10in 50 sheets
5
Hyperfilm βmax
Product description
Hyperfilm βmax is a specialist film for direct autoradiography. It is constructed from a clear plastic
base that is coated on one side with a high silver content emulsion, which is protected by an
Features anti-scratch layer. This film has an excellent linear range, very high resolution and high speed for
direct autoradiography. Due to its high silver content, this film must be processed manually.
and benefits
● High speed direct
autoradiography of 14C,
35S, 33P or 125I plastic base

● Fine grain globular emulsion
coated on one side ensures
emulsion
maximum resolution
● High silver content gives
excellent sensitivity and an
extended linear response for
accurate quantification
protective
● Clear base offers high anti-scratch layer
contrast against blackened
image for easy reading 14C, 35S
FIGURE 4
Hyperfilm βmax structure gives high resolution images
Applications
● Whole body autoradiography
● Receptor binding
FIGURE 5
● In situ hybridization In situ hybridization of NMDAR1 mRNA in the
adult rat brain. Negative film image from
Hyperfilm βmax using 35S labelled antisense
RNA probe
Data kindly provided by
Dr. Ryuichi Shigemoto and Professor Shigetada
Nakanishi, Kyoto University Faculty of Medicine, Japan
Hyperfilm βmax
Code Size Quantity
RPN9 18x24cm 25 sheets
6
Hyperfilm 3H
Product description
Hyperfilm 3H is a specialist film for the direct autoradiography of 3H.The single emulsion is supported
by a clear plastic base but has no anti-scratch layer. The anti-scratch layer on most films seriously
impedes or totally blocks the weak beta particles of 3H and the low energy Auger electrons of 125I
decay. As Hyperfilm 3H has no anti-scratch layer these weak particles can strike its emulsion directly
Features
and form an image. and benefits
● No anti-scratch layer facilitates
Since there is no protective layer, care must be taken when handling this film to avoid damaging
the sensitive detection of 3H
the emulsion and creating artefacts. This also means it cannot be put through automatic processors
by direct autoradiography
but must be developed by hand using standard manual X-ray developers and fixers. Hyperfilm 3H
● Each sheet is individually
is relatively insensitive to light and is therefore not recommended for use in fluorography or with
inspected ensuring uniform
intensifying screens.
coating
● Single side format and fine
globular crystals ensures
maximum resolution
plastic base
● Clear base offers high contrast
against blackened image for
emulsion easy reading
3H
FIGURE 6
Hyperfilm 3H structure with no protective layer
allows direct detection of 3H
Applications
● Whole body autoradiography FIGURE 7
Rat kidney.
● In situ hybridization Total 125I ANP binding showing renal blood
vessels, smooth muscle renal papilla, glomeri
● Receptor localization and other in cortex and the inner medulla. Reversed
image from Hyperfilm 3H
applications where there is a need
Photograph courtesy of John Wharton and
for the direct autoradiography Richard Rutherford, Dept. of Histochemistry,
of 3H and 125I Auger electrons Royal Postgraduate Medical School, London
Hyperfilm3H
Code Size Quantity
7
Hyperfilm ECL
Product description
The emulsion of this double coated film is extra blue light sensitive making it suitable for ECL™,
ECL Plus™, CDP-Star™ and other blue light chemiluminescent systems.
Features
and benefits spectral emission
log inverse exposure (µJ/sq cm) for net OD=1.0

2000 3.2
film sensitivity
● High speed detection of
3.1
chemiluminescence signal from
light intensity (arbitrary units)
nucleic acid and protein blots 1500 3.0
● System tested with ECL and 2.9
Gene Images kits to ensure 1000 2.8

optimum performance
2.7
● Coated with emulsion on
500 2.6
both sides to ensure optimum
sensitivity 2.5
● Clear base offers high contrast 0 0

340 360 380 400 420 440 480 500 520 540
wavelength (nm)
easy reading
● Can be processed in FIGURE 8
automatic processors or Emission spectrum of luminol enhanced chemiluminescence compared
with Hyperfilm ECL sensitivity profiles
manually using most
common X-ray film
developers and fixers
Applications
● Chemiluminescent Northern, Southern
and Western blots
FIGURE 9
Single-copy human gene detection with
a Gene Images™ labelled N-ras probe
Hyperfilm ECL
Code Size Quantity
8
Kodak BioMax MR
Product description
Kodak™ BioMax™ MR (maximum resolution) film, is a single emulsion film that utilizes T-Grain
technology. The single emulsion format increases resolution and background clarity, making it the ideal
choice for sequencing. It can be processed manually or in an automatic processor.
Standard emulsion Features

35
S Sample
and benefits
● High speed direct
autoradiography of samples
labelled with 14C, 35S and 33P
● Single coated emulsion, making
it the film of choice for
maximum resolution
● T-Grain emulsion gives two
Globular silver halide crystals Tabular grain silver halide crystals are flat, covering more fold improvement in sensitivity
cover less surface area surface area increasing number of interactions between beta over Kodak X-Omat™ AR film
particles and crystals
Large mass of crystals stop 35S beta particles,
for direct exposure of 14C, 35S
reducing efficiency per particle in exposing crystals Because crystals are flat each beta particle can expose multiple crystals
and 33P
FIGURE 10
BioMax emulsion increases sensitivity to 35S, 33P and 14C ● Clear base offers high contrast
easy reading and scanning
Applications
● 35S and 33P sequencing
BioMax MR-1 film (non-interleaved packaging)

Code Size Quantity
V8701302 8x10in 50 sheets
V8715187 35x43cm 50 sheets
V8736936 18x24cm 50 sheets
V8756447 18x43cm 50 sheets
V8912560 24x30cm 50 sheets
V8929655 30x40cm 50 sheets
FIGURE 11
V8809360 33x61cm 50 sheets Thermo Sequenase™ cycle
sequencing using Redivue™
nucleotides (33P). 2 hour exposure
on Kodak BioMax MR film
BioMax MR-2 film (Ready Pack – each sheet individually wrapped)
Code Size Quantity
V8567232 35x43cm 50 sheets
9
Autoradiography film
Kodak BioMax MS
Product description
Kodak Biomax MS
Kodak BioMax MS (maximum sensitivity) is a double sided film that utilizes Kodak T-Grain emulsion
technology to maximise interaction with beta particles (see Figure 10) and is designed for maximum
performance with 32P and 125I.When used with the BioMax MS or BioMax TranScreen™ HE intensifying
screens to detect 32P, BioMax MS film forms the fastest conventional autoradiography film and screen
Features system available. The emulsion is extremely light sensitive, in particular to the green light emissions of
and benefits BioMax intensifying screens. Note:This film should only be exposed to a red Kodak GBX-2 safelight filter
using a 7.5 or 15 watt bulb. It can be processed manually or in an automatic processor.
● State of the art technology,
the film of choice for
maximum sensitivity
4.0
● Reduces 4 day exposures
3.5
to 1 day and overnight
(total density – gross fog)
Net optical density
BioMax MS film/MS screen

3.0
exposures to 4 hours
2.5
● Detects 1 dpm bands in 1 day 2.0
● Can be processed in 1.5
XAR film/screen
automatic processors 1.0
BioMax Light and
XLS film/screen
or manually (using most 0.5
common X-ray film 0.0
developers and fixers) -0.3 0 0.3 0.6 0.9 1.2 1.5 1.8 2.1 2.4 2.7 3.0 3.3
Relative log activity, P (dpm), 40 hour exposure

32
FIGURE 13
Comparison of film sensitivity to 32P
Applications
Transcreen Generic screen/ ● 32P Northern and Southern blots
HE screen/ X-Omat AR film
BioMax MS film
13.8
BioMax MS-1 film (non-interleaved packaging)
Code Size Quantity
6.9 V8294985 8x10in 50 sheets
3.4 V1435726 35x43cm 50 sheets
V8222648 18x24cm 50 sheets
1.7
V1983063 18x43cm 50 sheets
V8326886 24x30cm 50 sheets
V8220774 30x40cm 50 sheets
0.86
dpm/band
100-hour exposure at -70ºC
FIGURE 12 BioMax MS-2 film (Ready Pack – each sheet individually wrapped)
Response to 32P Code Size Quantity
V1764505 35x43cm 50 sheets
10
Autoradiography film
Kodak BioMax Light
Product description
A double coated emulsion film from Kodak for the detection of chemiluminescence.
64 pg 64 pg 64 pg
Features
32 pg 32 pg 32 pg and benefits
● Suitable for the detection
16 pg 16 pg 16 pg
of chemiluminescence
● Can be processed in
8 pg 8 pg 8 pg
automatic processors
or manually using most
4 pg 4 pg 4 pg
common X-ray film
a b c developers and fixers
FIGURE 14
Comparison of BioMax film exposure to chemiluminescence.
Nucleic acid probe, Human β-Actin cDNA, exposure time 5 minutes.
(a) Kodak BioMax Light, (b) Kodak X-Omat AR, (c) Kodak BioMax MS
Applications
● Chemiluminescent blots
BioMax Light-1 (non-interleaved packaging)

Code Size Quantity
V8625170 35x43cm 50 sheets
V8194540 18x24cm 50 sheets
V1538966 24x30cm 50 sheets
V8122004 30x40cm 50 sheets
BioMax Light-2 film (Ready Pack – each sheet individually wrapped)

Code Size Quantity
V8946071 35x43cm 50 sheets
11
Kodak X-Omat AR (XAR)
Product description
Kodak X-Omat™ (XAR) is a general-purpose film for use with all commonly used isotopes. Coated
with emulsion on both sides, it provides high sensitivity for both direct exposure autoradiography and
exposures with the Hyperscreen™, BioMax MS or TranScreen intensifying screens.
Features Applications
and benefits ● Southern, Northern and Western blotting
● A popular general-purpose
film, compatible with 35S, 14C, X-Omat AR-5 film (alternate interleaved packaging)
33P, 32P and 125I Code Size Quantity
V1651512 35x43cm 50 sheets
V8532665 18x24cm 50 sheets
V8158982 18x43cm 50 sheets
V1501451 24x30cm 50 sheets
V1506955 30x40cm 50 sheets
V1531680 20cmx25m 1 roll
V1510478 14inx50ft 1 roll
X-Omat AR-2 film (Ready Pack – each sheet individually wrapped)

Code Size Quantity
V1651678 35x43cm 50 sheets
Kodak X-Omat LS (XLS)
Product description
Features Kodak X-Omat LS (XLS) is an economical, blue tinted film for use with all commonly used isotopes.
and benefits It provides significantly lower detection sensitivity than BioMax or XAR films resulting in longer
exposure times.
● Reduces costs where
sensitivity is not required
Applications
● Similar performance to other
● Southern and Western blotting, and sequences giving strong signals
‘blue’ tinted films
X-Omat LS-1 film (non-interleaved packaging)

Code Size Quantity
V8646770 8x10in (20.3x25.4cm) 50 sheets
V8926024 35x43cm (14x17in) 50 sheets
V1611342 5x7in (13x18cm) 50 sheets
V8688681 18x24cm (7x9.5in) 50 sheets
12
Autoradiography cassettes and screens
Hyperscreen intensifying screens
Product description
Hyperscreen™ intensifying screens are economical high performance calcium tungstate screens for use Features
with 32P and 125I.They can be used with pre-flashed film to give an increase in sensitivity while retaining
a linear response that allows quantitation of results.
and benefits
Hyperscreen
Applications ● For rapid results and high
● Southern, Northern and Western blots using 32P and 125I sensitivity when detecting 32P
and 125I, in Southern, Northern
– + ++
and Western blots
– minus ● Light emission from calcium
+ plus Hyperscreen
tungstate phosphor, fully
++ plus Hyperscreen
compatible with Hyperfilm MP
and pre-flashed
with Sensitize ● Use with Sensitize pre-flash
unit for even higher sensitivity
and accurate quantification
● Fine grain screens give clear
band resolution
● Available in a full range of sizes
to match Hypercassette and
Hyperfilm MP
Kodak intensifying
FIGURE 15
125I-labelled IgG slot blots – doubling dilutions screen cleaner
(100nCi to 1.56nCi) 2 hour exposure to ● Specially formulated solution
Hyperfilm MP
for dust and dirt removal and
static reduction, recommended
for all Kodak and Hyperscreen
intensifying screens
Hyperscreen
Code Size Quantity
RPN1662 18x24cm 1 pair
RPN1668 5x7in 1 pair
FIGURE 16
Intensifying
screen
Kodak intensifying screen cleaner and antistatic solution
cleaner and
Code Size Quantity antistatic
solution
V1064930 16oz 1 bottle
13
Kodak BioMax MS intensifying screens
Product description
Kodak BioMax MS intensifying screens are special screens that can be used with any autoradiography
film to increase sensitivity. Maximum sensitivity is achieved when used with BioMax MS film.
Features BioMax MR Film BioMax MS Film and MS Screen

and benefits 3H 14C 35S 33P 32P 32P & 125I
Sample
● Doubles the speed of
Emulsion
Overcoat
conventional film (Includes
Layer
Protective Dye) 7 mil
Estar Base
● When used with BioMax MS
Emulsion
film it will increase speed Layer
of detection four fold Intensifying

Screen
(compared to normal film
screen combinations)
● Uses conventional intensifying
FIGURE 18
screen format
Principle of direct autoradiography and normal intensifying screens
X-Omat AR BioMax MS
film/screen film/screen
Applications
● Southern, Northern and Western blots using 32P and 125I
Kodak BioMax MS intensifying screen

Code Size Quantity
V8142358 5x7in 1 screen
Before – NOW –
V8757270 18x24cm 1 screen
72 hours 16 hours
32P labelled slot blots exposed at -70°C
FIGURE 17 V8770331 30x40cm 1 screen
BioMax film and intensifying
screen: maximum speed for 32P
blotting applications
BioMax MS-1 film and screen combination pack (non-interleaved, 50 sheets per box with
1 BioMax MS intensifying screen)
Code Size Quantity
V1165893 8x10in 1 each
V1188077 35x43cm 1 each
V8462269 16x24cm 1 each
14
Kodak BioMax TranScreen intensifying screens
Product description
The BioMax TranScreen system offers a new, innovative technology, which unlike conventional screens
captures the beta particles prior to their being exposed to the film. The BioMax TranScreen then
converts the energy from the beta particles to photons without attenuation and directs the photons
to the film.
Features
TranScreen LE
and benefits
This is the first and only intensifying screen for 35S, 14C, 33P, 45Ca, 59Fe and even 3H labelled samples.
● TranScreen LE
Improves the resolution of 32P samples compared to traditional screens.
When used with BioMax MS
film it allows images of 35S,
TranScreen HE
14C, 33P, 45Ca, 59Fe and even
Innovative intensifying screen technology for 32P, 125I and high energy emitting radiolabels. 3H to be detected at least 5
times faster than conventional

Low Energy TranScreen LE High Energy TranScreen HE film screen systems
3H 14C 35S 33P 32P 32P & 125I Sample
Offers two fold better
Phosphor resolution for 32P and 125I
Base
images (compared to normal
intensifying screens)
BioMax MS Film
7 mil
Estar Base
● TranScreen HE
Increases sensitivity at least
Reflector four fold (compared to
normal intensifying screens)
FIGURE 19 Improved handling features
Principle of TranScreen intensifying screens. Note better resolution of TranScreen LE and image quality (compared
to BioMax MS screens)
Applications
Active side
e of blot
ett
● 3H and 14C thin layer chromatography rc ass
pe
Hy
● Southern, Northern and Western blots fil

m
S
M
ax
● Sequencing with 35S and 33P oM
Bi
Phoshor
TranScreen
Folder { Surface
Reflector surface
Kodak BioMax TranScreen LE FIGURE 20

Code Size Quantity Configuration of film, screen and
V1622034 8x10in 1 screen sample when in use.
Kodak BioMax TranScreen HE

Code Size Quantity
15
Hypercassette autoradiography cassettes
Product description
Features Hypercassette™ autoradiography cassettes have been designed for the toughest demands of life science
and benefits research. Deep cassettes are 1.5cm in depth allowing them to accommodate thicker samples.
● Light tight exposures
at temperatures as low
as –70°C
● Long life, chip proof surfaces
● Optimum resolution from
a design that ensures
maximum, even contact
between film and sample
● Minimal contamination as the
non-porous pad gives a wipe
clean interior
● Colour coding of work
possible, choice of
FIGURE 21
Hypercassette colours from Hypercassette autoradiography cassettes
red, blue or neutral
● Record details on
polycarbonate label and wipe
clean after use
Hypercassette, neutral (standard)

Code Size Quantity
RPN11642 18x24cm 1 cassette
RPN11645 35x43cm 1 cassette Hypercassette, blue (standard)
RPN11646 18x43cm 1 cassette Code Size Quantity
RPN11647 20x40cm 1 cassette RPN13642 18x24cm 1 cassette
RPN11648 5x7in 1 cassette RPN13643 24x30cm 1 cassette
RPN11649 8x10in 1 cassette RPN13645 35x43cm 1 cassette
RPN11650 10x12in 1 cassette RPN13649 8x10in 1 cassette
Hypercassette, red (standard)

Code Size Quantity Hypercassette, neutral (deep)
RPN12642 18x24cm 1 cassette Code Size Quantity

RPN12649 8x10in 1 cassette RPN11629 8x10in 1 cassette
16
Autoradiography accessories
Amplify fluorographic reagent
Product description
Amplify ™ is a fast, convenient fluorographic reagent for 35S, 14C and 3H labelled samples in gels.
Features
Increased sensitivity can be achieved in addition to considerably reducing exposure times of these
weak beta emitters.
and benefits
● Increased detection efficiency
– 1000 fold for tritium
– 15 fold for 35S and 14C
● Less than 2 hours total
emulsion
plastic base plastic base preparation time
emulsion ● Odourless, does not
anti-scratch anti-scratch
layer layer contain DMSO or other
gel gel organic solvents
3H/Auger 3H/Auger
● Versatile, can be used with
FIGURE 22 FIGURE 23 acrylamide or agarose gels
Internal absorption of weak β-emissions Gel impregnated with fluorographic reagent with no change in protocol
within a sample such as Amplify. Weak β-emissions are converted
to light and efficiently recorded on film ● Fully compatible with Sensitize
and Hyperfilm MP
35 3
S H
+ – + –
FIGURE 24
Autoradiographs showing the detection of 35S- and
3H-labelled proteins in acrylamide gels with (+) and without (–)
Amplify. Samples exposed to pre-flashed Hyperfilm MP
Amplify fluorographic reagent

Code Size Quantity
NAMP100 1 litre 1 pack
17
Darkroom lamp
Product description
Features The darkroom lamp is a socket type metal lamp that provides direct illumination. It screws into either
and benefits a wall or drop-cord socket and holds a 5.5 inch diameter filter and a 7.5 watt standard incandescent
bulb. The GBX-2 safelight filter is compatible with all autoradiography films including the highly light
● Socket type metal lamp that
provides direct illumination sensitive BioMax MS film. A 7.5 or 15 watt bulb is recommended.
FIGURE 25
Darkroom lamp
Darkroom lamp
Code Size Quantity
V1521178 14cm (51/ 2 in) diameter filter 1 lamp
Note: Bulb and filter not included with darkroom lamp
Safelight filter, GBX-2

Code Size Quantity
V1416627 14cm (51/ 2 in) diameter 1 filter
Hypertorch
Product description
Features Hypertorch™ is a red light darkroom torch. A red LED produces a safe wavelength of light for short
and benefits exposures to film. It allows visibility in areas of the darkroom, where fixed safe lights cannot reach e.g.
cupboards. Ideal for use with Hyperfilm, Kodak autoradiography films and emulsions.
● Local illumination, minimizes film
background
● Red LED, compatible with most FIGURE 26

autoradiography films Hypertorch – battery-powered,
darkroom pen torch
● Portable, allows all areas of
darkroom to be illuminated
● Spring switch prevents accidental

Hypertorch
exposures
Code Size Quantity
RPN1620 3 torches 1 pack
(includes 2 AAA batteries)
18
ECL mini camera
Product description TM
The ECL™ mini camera is a camera luminometer specifically designed for use with ECL systems in
particular Western blots from mini gels. It allows the chemiluminescent signal to be captured on
Polaroid™ film without the need of a darkroom.
Polaroid™ film 667 is recommended for routine work while 612 can be used if greater sensitivity is
needed. Polaroid™ film is not supplied with the ECL mini camera. For work that requires the highest
Features
sensitivity and resolution use Hyperfilm ECL and conventional methodology. and benefits
● Instant results at the bench
on Polaroid film
● Compact and portable
FIGURE 27
ECL mini-camera ● Handles blots up to a
size of 52x77mm
Applications
● Chemiluminescent blots from mini gels
FIGURE 28
Doubling dilution (31, 15.5, 7.8, 3.9ng) of mouse
IgG detected with anti-mouse Ig HRP and ECL Plus.
Exposed for 1 minute
ECL mini camera

Code Quantity
RPN2069 1 camera
(Supplied with 5 sample boats)
Replacement sample boats

Code Quantity
RPN2079 50 boats
19
Hypercoat nuclear emulsions
Product description
Features Hypercoat™ EM-1 and Hypercoat LM-1 are ready to use nuclear emulsions that can be used in the
and benefits microautoradiography of radiolabels in tissues or cells. When using 3H the low energy of this isotope
gives results of the highest resolution best appreciated using Hypercoat EM-1 which produces grains of
● Ready to use, simply melt
before dipping and coating a fine size. Isotopes such as 14C, 35S, 33P, 32P and 125I are best detected with Hypercoat LM-1 which has
a slightly larger grain size optimized for use with higher energy emissions. Both emulsions come ready-
● High resolution, uniform
to-use and do not require diluting, a process that can increase backgrounds. Store in the dark at 2-8°C.
crystal size and aggregate free
Compatible with Hypercoat dipping vessels, specially designed glass vessels that facilitate maximum slide
● Crystal sizes to suit all
coverage from small volumes of emulsions.
applications
– 0.2µm for general
microautoradiography
(Hypercoat LM-1)
– 0.13µm for high resolution
light or electron
microscopy
of 3H (Hypercoat EM-1)
● Maximum sensitivity, high
silver content allows reduced
exposure times (0.09g Ag/g
emulsion)
FIGURE 30
Amersham Pharmacia Biotech Hypercoat nuclear emulsions
and Hypercoat dipping vessel
Applications
● Microautoradiography of tissues and cells
Hypercoat LM-1
FIGURE 29 Code Size Quantity
Binding of [125I]endothelin-1
to artery wall of patient with RPN40 2x20ml 1 pack
ischaemic heart disease, visualized
with Hypercoat LM-1 Hypercoat EM-1
Photograph courtesy of Dr M Dashwood, Code Size Quantity
Department of Physiology, Royal Free Hospital
School of Medicine, London RPN41 2x20ml 1 pack
Hypercoat dipping vessels

Code Size Quantity
RPN39 1 pack 3 vessels
20
Hyperprocessor automatic film processor
Product description
Hyperprocessor™ is an advanced automatic film processor of compact bench top design. It is designed
Features
to give consistent high quality autoradiographs simply and quickly with Hyperfilm MP, Hyperfilm ECL,
BioMax, XAR, XLS and most standard X-ray films.
and benefits
● Simple to operate. Develops,
Sensors measure the film throughput and replenish chemicals as needed. Chemicals are maintained fixes, washes and dries
at optimum temperatures for quality results. automatically in 21/2 mins
● Reproducible results. Internal
control systems ensure
consistent high performance
● Compact design, requires less
than 0.2m2 floor space
FIGURE 31
Hyperprocessor ● Versatile. Can handle film sizes
from 10x10cm up to 35x43cm
● High throughput. Can process
70 films, size 24x30cm, per hour
Contents
Component Quantity
Trolley 1
Reservoirs 2
Small spares kit 1
Full operator manual 1
Full installation manual 1
Applications
● Automatic rapid processing of autoradiography films
ADDITIONAL INFORMATION
Note: Hyperprocessor is not available in certain
Hyperprocessor countries. Please contact your local office for
further details.
Code Description Quantity
RPN1700 Hyperprocessor, 220–240v 1 processor
RPN1700A Hyperprocessor, 110–120v 1 processor
21
Liquid scintillation cocktail
Product description
Features A comprehensive range of liquid scintillation cocktails compatible with most sample types.
and benefits
● For aqueous samples:
– ACS - Organic scintillant.
Efficiencies with tritium of
up to 50%, provided sample
added is less than 6% (v/v).
Flashpoint 27.2°C.
– ACSII - Organic scintillant.
Efficiencies with tritium of up
to 50%, provided sample
added is less than 4.5% (v/v).
A flashpoint of 48.9°C
reduces handling hazards.
FIGURE 32
– BCS - Biodegradable
Liquid scintillation cocktail specifications
scintillant. Efficiencies with
tritium of 30-40%. Low odour,
low toxicity and a high
flashpoint (>150°C) makes
it less hazardous to the user ACS scintillation cocktail
and the environment.
Code Quantity
– PCS - Wide sample range
phase-combining scintillant. NACS104 4x4litres
Efficiencies with tritium of
30-50%. Flashpoint 27.2°C. ACS-II scintillation cocktail
Code Quantity
● For non-aqueous samples: NACS204 4x4litres
– BCS-NA - Biodegradable
scintillant. Efficiencies with
BCS scintillation cocktail
tritium of up to 45%. Low
odour, low toxicity and a high Code Quantity
flashpoint (>150°C) makes NBCS104 4x4litres
it less hazardous to the user
and the environment. PCS scintillation cocktail
– OCS – High efficiency xylene-
Code Quantity
based scintillant. Efficiency
with tritium of >50%. NPCS104 4x4litres
Flashpoint 26.7°C.
BCS-NA scintillation cocktail
● Tissue solubilizer: Code Quantity
– NCS – Rapid and efficient NBCS204 4x4litres
solubilizer for biological
materials and polyacrylamide
OCS scintillation cocktail
gels prior to liquid scintillation
Code Quantity
counting. Compatible with
BCS-NA and OCS scintillation NOCS104 4x4litres
cocktails
NCS tissue solubilizer
These products are not available in certain Code Quantity
countries, please contact your local office
for availability.
NNCS502 4x4litres
22
To help you choose…
23
Microscale autoradiography standards
Product description
Features Microscale ™ standards are reference sources for autoradiography.The 3H, 14C or 125I activity has been
and benefits arranged in eight or ten layers of polymer, to give a good working range.
● Safe and easy to use as the [3H]microscales [14C]microscales [125I]microscales
radioisotope exists as a stable RPA506 RPA507 RPA504 RPA511 RPA523
Bq/mg nCi/mg Bq/mg nCi/mg kBq/g nCi/g Bq/g nCi/g kBq/mg nCi/mg
polymer
4048.0 109.4 588.0 15.9 31.89 862 3635.0 98.26 23.9 646
● Activity is uniformly 2468.0 66.7 290.0 7.84 25.90 700 1793.0 48.46 11.9 321
incorporated into the polymer 1487.0 40.2 180.0 4.87 19.39 524 888.7 24.02 5.85 158
872.8 23.6 74.4 2.01 12.91 349 352.6 9.53 3.0 81

● Arranged in 8 to 10 layers of
525.4 33.5 37.0 1.00 8.55 231 177.2 4.790 1.33 36
increasing specific activity, to
320.4 8.7 17.8 0.48 4.44 120 87.21 2.357 0.74 20
provide a good working range
175.0 4.7 9.62 0.26 2.18 59 33.63 0.909 0.37 10
110.6 3.0 3.74 0.10 1.11 30 16.02 0.433 0.21 5.6
9.29 0.251 0.095 2.56
3.70 0.100 0.044 1.20

FIGURE 33
Typical polymer activity
Applications
● Whole body autoradiography
● Receptor localization and receptor binding
Autoradiographic [3H] microscale

RPA506 0.111–4.07kBq/mg, 3–110nCi/mg 20 strips (19mmx3.5mmx50µm)
RPA507 3.7–592Bq/mg, 0.1–16nCi/mg 20 strips (19mmx3.5mmx50µm)
RPA510 10 strips RPA506 + 10 strips RPA 507 10 + 10 strips
Autoradiographic [14C] microscale

RPA504 1.15–32.7kBq/g, 31–883nCi/g 3 strips (19mmx3.5mmx120µm)
RPA504L 1.15–32.7kBq/g, 31–883nCi/g 20 strips (19mmx3.5mmx120µm)
RPA511 3.7–3700Bq/g, 0.1–100nCi/g 3 strips (21mmx3.5mmx120µm)
RPA511L 3.7–3700Bq/g, 0.1–100nCi/g 20 strips (21mmx3.5mmx120µm)
Autoradiographic [125I] microscale

RPA522 0.046-23.7kBq/mg, 1.25-640nCi/mg 1 block (25mmx4.5mmx25µm)
RPA523 0.046-23.7kBq/mg, 1.25-640nCi/mg 3 strips (25mmx4.5mmx20µm)
RPA523L 0.046-23.7kBq/mg, 1.25-640nCi/mg 20 strips (25mmx4.5mmx20µm)
24
Sensitize preflash unit
Product description
Sensitize™ is a specially designed flashgun for use with Hyperfilm ECL or Hyperfilm MP in conjunction
Features
with Hyperscreen intensifying screens to improve sensitivity and quantitation. It improves the linear
range and increases film sensitivity.
and benefits
● Most effective when detecting
small amounts of radioactivity
and chemiluminescent signal
● Calibration protocol ensures
reproducibility
● High quality orange filter
for optimum wavelength
● Sealed unit guards against
white light leakage, often
a problem with a
conventional flashgun
FIGURE 34
Sensitize unit
+ –
0.56kb
FIGURE 35
Lambda Hind III digests (10-fold dilutions) ECL detected and exposed to
Hyperfilm ECL. Note the presence of 0.56kb fragment after pre-flashing
Sensitize preflash unit

Code Quantity
RPN2051 1 unit
25
Processing hanger
Product description
Features Clip-type metal film hanger for manual deep tank development of sheet films.
and benefits
● Design ensures optimum
film tension
● Accurate alignment of pin
hole clamps permits easier
washing near clips
FIGURE 36
Processing hanger
Processing hangers
Code Size Quantity
V1502723 5x7in 1 hanger
V1502764 8x10in 1 hanger
V1502822 35x43cm 1 hanger
TrackerTape
Product description
Features TrackerTape ™ is a phosphorescent tape which, when written on, can be used to label samples and
and benefits corresponding autoradiographs.
● Produces a permanent, FIGURE 37
negative image on Western blot identified
autoradiographs by TrackerTape
● Avoids confusion by
allowing samples to be
matched to results
● Can be used with ordinary
marker pen
● Quick to use, image
is produced in minutes
Applications
● Southern, Western and Northern blots
Note: Not available in the USA.
TrackerTape
Code Size Quantity
26
Other film products
Kodak 35mm black-and-white films
Product description
Technical Pan film (TP) Features
Outstanding choice for photomicography and other scientific and industrial applications where high
definition records are required. Suitable for making high quality enlargements of up to 50x, making
and benefits
● Comprehensive range offering
continuous tone originals or copy negatives, and making reverse text title slides.
a variety of contrast levels and
T-Max 100 Professional film (TMX) film speeds
A continuous tone, panchromatic black-and-white film for general pictorial or lab documentation
imaging. It is especially useful for detailed subjects where outstanding image quality is required. Features
medium speed (EI 100) extremely fine grain and very high resolving power.
T-Max 400 Professional film (TMY)

A continuous tone, panchromatic black-and-white negative film that is especially useful for
photographing dimly lit subjects, fast action, extended range flash pictures, and subjects that require
good depth of field. This film is highly useful for scientific and biomedical work, especially when
fluorescence photography is required. Can be exposed at a speed of EI 800 with normal processing, or
FIGURE 38
at EI 1600 with push-processing to obtain excellent results. Frog’s blood, magnification 450x on
T-MAX 400 Professional film
T-Max P-3200 Professional film (TMZ)
A multispeed black-and-white film, especially useful for very fast action and dimly lit subjects where
auxiliary illumination is not practical.The ability to expose this film at exceptionally high speeds (EI 3200
to 25,000) allows you to take pictures in many situations where photography was previously impossible.
Photomicrography of extremely weak fluorescence may require the great speed and increased contrast
this film displays when push-processed at EI 6400 or higher, but for most fluorescence the finer grain of
T-Max 400 is the better choice.
Ektagraphic HC slide film (HCS)

Orthochromatic high contrast film designed for copying line drawings and text for making reverse-text
black-and-white title slides.
Technical Pan film (TP)

Code Size Quantity
V1297563 36 exposures 20 rolls
T-Max 100 film (TMX)

Code Size Quantity
T-Max 400 film (TMY)

Code Size Quantity
T-Max P3200 film (TMZ)

Code Size Quantity
Ektagraphic HC slide film (HCS)

Code Size Quantity
27
Other film products
Kodak 35mm colour slide films
Product description
Features Ektachrome™ 100 Professional film (EPN)
and benefits An excellent choice for photographic applications that demand precision colour reproduction. Intended for
exposure with daylight or flash (5500 K) illumination. Colour transparencies produced on this film exhibit
● Accurate colour reproduction
very high sharpness and very fine grain. Suitable for general pictorial photography and lab documentation.
in images produced under
daylight or tungsten illumination Ektachrome 64T Professional film (EPY)
A medium-speed colour reversal film that features excellent colour reproduction, very fine grain, and
very fine sharpness. It is designed for exposure with tungsten illumination (3200 K). An excellent choice
for general use photomicrography on microscopes with tungsten-halogen or ordinary tungsten lamps.
Ektachrome 160T Professional film (EPT)
A medium-speed (ISO 160) colour reversal film that is designed for tungsten illumination (3200 K) and
features very fine grain and high sharpness. Well-suited for the photography of specimens (particularly
three-dimensional ones) by reflected light in the stereo microscope.
FIGURE 39 Ektachrome 320T Professional film (EPJ)

Botanical subject, stained with A fast colour reversal film specifically balanced for exposure with tungsten illumination (3200 K). Its high
fast green and safrinin, magnification speed (ISO 320) makes it a useful film for situations that require small apertures for good depth of field.
15x on Ektachrome 64T
Useful for fluorescence photomicrography of blue-emitting fluorochromes.
Professional film
Ektachrome P1600 Professional film (EPH)
Designed for low-light level photography, this high-speed colour reversal film is optimised for an effective
speed of EI 1600 using a two-stop push with process E6-P. Featuring high sharpness, fine grain, and bold,
saturated colours, this film is ideally suited for singularly labelled and doubly labelled fluorescence specimens.
Vericolor slide film (SO-279)
Optimised for producing colour slides directly from colour negatives and producing reverse-text colour
slide presentations.
FIGURE 40
Algae, wet mount, magnification Ektachrome 100 Professional (EPN) colour slide film
8x on Ektachrome 64T
Code Size Quantity
Professional film
Ektachrome 64T Professional (EPY) colour slide film

Code Size Quantity
Ektachrome 160T Professional (EPT) colour slide film

Code Size Quantity
Ektachrome 320T Professional (EPJ) colour slide film

Code Size Quantity
Ektachrome P1600 Professional (EPH) colour slide film

Code Size Quantity
Vericolor (SO-279) colour slide film

Code Size Quantity
28
Other film products
Kodak Duplicating film
Product description
A range of duplicating products which facilitate the generation of direct positive film transparencies
of electrophoresis gels.
Features
Applications
● Direct exposure film (DEF) – double-sided speciality film for X-ray diffraction analysis
and benefits
●
DEF film
RA duplicating film – single-emulsion film on a clear base that enables same-size reproductions
of autoradiographs ● Emulsion layer on both sides
for increased sensitivity
● Clear polyester for crisp black
and white images
RA duplicating film
● Slower film for enhanced
image quality and film stabiltly
FIGURE 41
32P labelled blot reproduced
on RA duplicating film
DEF film
Code Size Quantity
V1559145 12.5x12.5cm 50 sheets
V1558162 35mmx20m 1 roll
RA duplicating film
Code Size Quantity
V1429760 8x10in 100 sheets
V8165938 28x35cm 100 sheets
V1389923 35x43cm 100 sheets
29
Other film products
Kodak micro-autoradiography & TEM products
Product description
Features A specially engineered range of micro-autoradiography products that facilitate high-quality images in
and benefits Transmission Electron Micrography (TEM) and electron diffraction studies. Each of these products offers
considerable latitude in performance, but are best suited for medium-energy electrons (40 to 100kV)
● Suitable for medium energy
electrons (40-100kV) though acceptable results can also be obtained at higher electron-energy levels.
● Range offers flexibility to meet Kodak electron microscope film 4489

most user needs
This film has approximately half the speed of SO-163 film but exhibits less curl and shorter pump-down times.
● Films have gelatin overcoat to
minimise abrasion and a clean Kodak electron image film SO-163
gelatin backing to reduce curl Approximately two times faster than 4489 film, however it requires longer pump-down times and
shows greater film curl. When using SO-163 film for the first time, recalibration of the microscope
exposure device is recommended.
FIGURE 42
The Kodak TEM range
Electron microscope film 4489

Code Size Quantity
V1613108 3.25 x 4in 100 sheets
V1662238 3.25 x 4in 250 sheets
V1615228 6.5 x 9cm 100 sheets
V8971160 6.5 x 9cm 250 sheets
V1725852 8.3 x 11.9cm 100 sheets
V1608124 7.0 x 7.0cm 100 sheets
Electron image film SO-163

Code Size Quantity
V1679257 3.25 x 4in 100 sheets
V8010100 3.25 x 4in 250 sheets
V1679273 6.5 x 9.0cm 100 sheets
V8826018 6.5 x 9.0cm 250 sheets
30
Amplify, ECL, ECL device, ECL Plus, Hypercassette,
Hypercoat, Hyperfilm, Hyperprocessor, Hyperscreen,
Hypertorch, Microscale, Sensitize, Thermo Sequenase and
Trackertape are trademarks of Amersham Pharmacia
Biotech Limited or its subsidiaries.
Amersham is a trademark of Nycomed Amersham plc.
Pharmacia and Drop Design are trademarks of Pharmacia
& Upjohn Inc.
Kodak, BioMax, Transcreen, Ektachrome and X-Omat are
trademarks of Eastman Kodak Company
Polaroid is a trademark of Polaroid (UK) Limited
CDP-Star is a trademark of Tropix Inc and protected under
one or more of US patents numbers 5326882 and 4931569
All goods and services are sold subject to the terms and
conditions of sale of the company within the Amersham
Pharmacia Biotech group which supplies them. A copy of
these terms and conditions is available on request.
© Amersham Pharmacia Biotech UK Limited 1998 – All
rights reserved.
Amersham Pharmacia Biotech UK Limited
Amersham Place Little Chalfont Buckinghamshire
HP7 9NA England
Amersham Pharmacia Biotech AB SE-751 84
Uppsala Sweden
Amersham Pharmacia Biotech Inc 800 Centennial
Avenue Piscataway NJ 08855-1327 USA
For more information about
Amersham Pharmacia Biotech
visit our website:
www.apbiotech.com
Asia Pacific Finland Russian Federation
Amersham Pharmacia Biotech Asia Pacific Ltd Amersham Pharmacia Biotech Europe GmbH ZAO Amersham Pharmacia Biotech
Tel: +852 2811 8693 Tel: 358 (9) 512 3940 Tel: +7 (095) 232 0250, 956 11 37
Fax: +852 2811 5251 Fax: 358 (9) 512 1710 Fax: +7 (095) 232 6377
Australasia Germany South East Asia
Amersham Pharmacia Biotech Australia Pty Ltd Amersham Pharmacia Biotech Europe GmbH Amersham Pharmacia Biotech AB
Tel: +61 2 9894 5188 Tel: +49 0761 4903 229 Tel: 60 3 724 2080
Fax: +61 2 9899 7511 Fax: +49 0761 4903 405 Fax: 60 3 724 2090
Austria Italy South East Europe
Amersham Pharmacia Biotech Europe GmbH Amersham Pharmacia Biotech Italia Amersham Pharmacia Biotech GmbH
Tel: 01 576 0616 25 Tel: 02 27322 1 Tel: +43 (1) 982 3826
Fax: 01 576 0616 27 Fax: 02 27302 212 Fax: +43 (1) 985 8327
Belgium Japan Spain
Amersham Pharmacia Biotech Benelux Amersham Pharmacia Biotech KK Amersham Pharmacia Biotech Europe GmbH
Tel: 0800 73888 Tel: 81 3 5331 9317 Tel: 93 594 4950
Fax: +32 3272 1637 Fax: 81 3 5331 9372 Fax: 93 594 4955
Canada Latin America Sweden
Amersham Pharmacia Biotech, Inc. Amersham Pharmacia Biotech do Brasil Ltda Amersham Pharmacia Biotech Europe GmbH
Tel: 1 800 463 5800 Tel: 0800 136 833 Tel: 018 16 40 00
Fax: 1 800 567 1008 Fax: +55 11 3667 8799 Fax: 018 71 24 44
Central Europe Middle East and Africa Switzerland
Amersham Pharmacia Biotech Export GmbH Amersham Pharmacia Biotech Export GmbH Amersham Pharmacia Biotech Europe GmbH
Tel: +43 1 982 3826 Tel: +30 (1) 96 00 687 Tel: 01 802 81 50
Fax: +43 1 985 8327 Fax: +30 (1) 96 00 693 Fax: 01 802 81 51
Denmark Netherlands UK
Amersham Pharmacia Biotech Europe GmbH Amersham Pharmacia Biotech Benelux Amersham Pharmacia Biotech UK Limited
Tel: +45 4516 2400 Tel: 0165 580 410 Tel: 0800 515 313
Fax: +45 4516 2424 Fax: 0165 580 401 Fax: 0800 616 927
France USA
Norway
Amersham Pharmacia Biotech Europe GmbH Amersham Pharmacia Biotech Inc
Amersham Pharmacia Biotech Europe GmbH
Amersham products Tel: +1 800 526 3593
Tel: 63 89 23 10
Tel: +33 169 18 28 00 Fax: +1 800 329 3593
Fax: 63 89 23 15
Fax: +33 169 29 00 52
Pharmacia Biotech products
Tel: +33 169 35 67 00
Fax: +33 169 41 96 77
S1247/7273/97/09

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The Complete

As well as our own well-established autoradiography range, the company is also

a worldwide distributor of Kodak™ Scientific Imaging Systems’ autoradiography and

imaging products. Amersham Pharmacia Biotech is therefore able to advise customers on

products from a single source.

In addition to this catalogue, Review No.23, Efficient Detection of Bio-molecules by

Autoradiography, Fluorography or Chemiluminesence, by R. Laskey, is also available.

Review 23 is an independently written reference, that describes in detail, the principles

of indirect and direct autoradiography.

Review 23 is available on request, please contact your

local sales office.

Southern/Northern blots high speed and sensitivity 32P

Plaque/colony lifts accurate quantitation

optimum resolution 33P/35S

non-radioactive, quantification ECL, Gene Images

non-radioactive, quantification ECL, Gene Images

optimum resolution and maximum 35S

speed for difficult sequences

speed for difficult sequences

optimum resolution 125I

Western blots maximum speed and sensitivity 125I

non-radioactive/maximum sensitivity ECL

– high resolution 33P/35S

localization subcellular level:

– high resolution 125I/3H

Whole body optimum resolution 125I/3H

autoradiography high resolution 35S/14C

Pre-flash and screens at -70ºC Hyperfilm MP Sensitize, Hyperscreen

Screens at -70ºC BioMax MS BioMax TranScreen HE

Screens at -70ºC BioMax MS film BioMax TranScreen HE

Direct autoradiography Hyperfilm MP

Direct autoradiography BioMax MR

Direct autoradiography Hyperfilm MP

Pre-flash and fluorography at -70ºC Hyperfilm MP Amplify, Sensitize

Direct autoradiogaphy Hyperfilm βmax/BioMax MR

Direct autoradiography Hyperfilm 3H

MS intensifying screens radioactive emissions

● Confidence when sharing a

35S, 33P or 125I plastic base

log inverse exposure (µJ/sq cm) for net OD=1.0

nucleic acid and protein blots 1500 3.0

● System tested with ECL and 2.9

Gene Images kits to ensure 1000 2.8

● Clear base offers high contrast 0 0

Standard emulsion Features

BioMax MR-1 film (non-interleaved packaging)

BioMax MS film/MS screen

Relative log activity, P (dpm), 40 hour exposure

BioMax Light-1 (non-interleaved packaging)

BioMax Light-2 film (Ready Pack – each sheet individually wrapped)

X-Omat AR-2 film (Ready Pack – each sheet individually wrapped)

Kodak X-Omat LS (XLS)

X-Omat LS-1 film (non-interleaved packaging)

Features BioMax MR Film BioMax MS Film and MS Screen

of detection four fold Intensifying

Kodak BioMax MS intensifying screen

times faster than conventional

● Southern, Northern and Western blots fil

Kodak BioMax TranScreen LE FIGURE 20