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Positive Affect, Psychological Well-Being, and Good Sleep: Andrew Steptoe, Katie O'Donnell, Michael Marmot, Jane Wardle
Positive Affect, Psychological Well-Being, and Good Sleep: Andrew Steptoe, Katie O'Donnell, Michael Marmot, Jane Wardle
Abstract
Objective: To discover whether positive affect and purpose in including financial strain, social isolation, low emotional support,
life (eudaimonic well-being) are associated with good sleep negative social interactions, and psychological distress were also
independently of health problems and socioeconomic status, and related to reported sleep problems. The strength of these
to evaluate their role in mediating the influence of psychosocial associations was reduced by 20–73% when positive affect and
risk factors on poor sleep. Methods: A cross-sectional study was eudaimonic well-being were taken into account, suggesting that
carried out with 736 men and women aged 58–72 years, with effects were partly mediated by positive psychological states.
positive affect assessed by aggregating ecological momentary Conclusions: These results suggest that both positive affect and
samples. Sleep problems were assessed with the Jenkins Sleep eudaimonic well-being are directly associated with good sleep and
Problems Scale, and psychosocial risk factors were measured by may buffer the impact of psychosocial risk factors. The relation-
standardized questionnaires. Results: Both positive affect and ships are likely to be bidirectional, with disturbed sleep engender-
eudaimonic well-being were inversely associated with sleep ing lower positive affect and reduced psychological well-being,
problems after adjustment for age, gender, household income, and positive psychological states promoting better sleep.
and self-rated health (P b.001). Negative psychosocial factors © 2008 Elsevier Inc. All rights reserved.
Keywords: Sleep disturbance; Positive affect; Well-being; Stress; Socioeconomic status
0022-3999/08/$ – see front matter © 2008 Elsevier Inc. All rights reserved.
doi:10.1016/j.jpsychores.2007.11.008
410 A. Steptoe et al. / Journal of Psychosomatic Research 64 (2008) 409–415
aspects of eudaimonic well-being, including purpose in life, None had taken part in previous investigations of positive
environmental mastery, and positive relationships, were affect or psychological well-being. They were a subset of the
associated with good sleep in a cohort of older adults. 6914 men and women who participated in the 18- to 19-year
No study has yet investigated the relationships between follow-up in 2003–2004 and were recruited for a substudy of
sleep disturbance and both types of positive psychological heat shock proteins [24]. Ninety-one respondents were
state. The first aim of the present study was therefore to excluded from these analyses because they were taking
examine the associations between self-reported good (undis- psychotropic medication or had a history of coronary heart
turbed) sleep and both positive affect and eudaimonic well- disease or stroke, leaving 736 participants.
being in a healthy middle-aged and older sample. Sleep
problems are more common in people of lower socioeconomic 2.2. Measures
status (SES) as defined by income, education, and occupational
status [17–19]. Additionally, disturbed sleep is associated both Sleep problems were assessed using the Jenkins Sleep
with chronic medical conditions and poor self-rated health Problems Scale [25], a widely used brief self-report
[2,3,17]. Since SES and health may affect positive psycholo- instrument [26]. This contains items assessing number of
gical states as well, these factors were taken into account in the times waking up in the night, difficulty staying asleep,
analyses of relationships with good sleep. trouble falling asleep, etc. The Cronbach α in this population
Psychosocial factors may also be related to sleep was .84. Scores were scaled from 0 to 100, with higher
disturbance and impaired positive well-being. General psy- scores indicating greater sleep problems.
chological distress, depression, and anxiety have all been Positive affect was measured using ecological momentary
associated with sleep problems and insomnia [2,4,17]. assessment methods rather than a measure administered at a
People who report greater chronic life stress including single time point [6]. Four ratings were requested for 2.5, 8,
general stress, financial difficulties, and work strain and 12 h after waking, and at bedtime. Actual times of
experience more disturbed sleep [4,19,20]. Sleep quality is assessment averaged 9:18 a.m.±60 min, 2:45 p.m.±67 min,
impaired in individuals who are socially isolated and in 6:54 p.m.±67 min, and 11:09 p.m.±58 min. On each occasion,
people who describe unsatisfactory social relationships participants were asked “how happy, excited or content do
[4,21]. These factors may combine to generate a high level you feel at this moment”, with four response options: ‘not at
of psychosocial adversity in people experiencing chronic life all’, ‘somewhat’, ‘very much’, and ‘extremely’.
stress coupled with low social support. For example, in a Eudaimonic well-being was measured with three scales
study of middle-aged working men and women, Steptoe and from the CASP-19 [27]. The CASP-19 was devised as a
Marmot [22] showed that sleep problems were more needs satisfaction quality of life measure for people in early
common in people reporting high work, financial, and old age and consists of four scales: control, autonomy,
neighborhood stress coupled with low emotional support and pleasure, and self-realization. The control, autonomy, and
social connectedness. self-realization scales correspond to the concepts of environ-
People who experience psychological distress, chronic mental mastery, autonomy, self-acceptance, and purpose in
life stress, low emotional support, and social isolation may life that make up psychological well-being in Ryff's
be less happy and less fulfilled in their lives than others. But taxonomy [11,16]. Each item is scored on a four-point
positive psychological states may also act as protective scale from never to often. Control was assessed by six items
factors, buffering the impact of psychological distress and (e.g., “I feel that what happens to me is out of my control”),
adversity on health outcomes. Consequently, associations autonomy with five items (e.g., “I feel that I can please
between psychosocial risk factors and poor sleep may be myself in what I do”), and self-realization with four items
modified by positive psychological states. The second aim of (e.g., “I feel satisfied with the way my life has turned out”).
this study was to test this possibility by examining whether The Cronbach α for these three scales ranged from 0.60 to
relationships between psychosocial risk factors and sleep are 0.85. Scores on the three scales were averaged and scaled
independent of positive psychological states, or are attenu- from 0 (lowest) to 100 (highest).
ated when these factors are taken into account. We measured Household income was assessed as a measure of SES.
life stress, social relationships, and psychological distress, Participants were asked to estimate their total household
and tested the effect of adding positive affect and eudaimonic income and were categorized into low (b£25,000), medium
well-being into the regressions on sleep problems. (£25–50,000), and higher (N£50,000) income groups. Self-
rated health was measured with the item “In general, would
you say your health is: excellent, very good, good, fair, or
2. Method poor”. Participants were also classified on whether they were
currently in paid employment.
2.1. Participants The psychosocial risk factors tested in these analyses
were indicators of chronic life stress, social relationships,
Participants were 827 men and women who were and psychological distress. Two aspects of chronic life stress
members of the Whitehall II epidemiological cohort [23]. were assessed. Financial strain was measured with the items:
A. Steptoe et al. / Journal of Psychosomatic Research 64 (2008) 409–415 411
Table 1 you feel good about yourself”, α=.87), while negative social
Characteristics of study participants interactions were measured with four items (e.g., “how much
Men (n=486) Women (n=250) P in the last twelve months did talking to this person make
Age (years) 61.0±5.9 60.4±5.7 .19 things worse?”, α=.68). Responses were scaled from
Currently employed (n=730) 272 (56.3%) 132 (53.4%) .38 0=lowest to 100=highest. Finally, psychological distress
Household income (n=707) was measured with the General Health Questionnaire (GHQ)
b £25,000 155 (32.7%) 106 (45.5%) .001
30, a widely used screening questionnaire for psychiatric
£25–50,000 179 (37.8%) 85 (36.5%)
N£50,000 140 (29.5%) 42 (18.0%) disorder suitable for population studies. Scores could range
Self-rated health (n=734) from 0 to 30, and the Cronbach α was .95.
Excellent 64 (13.2%) 20 (8.0%) .001
Very good 197 (40.6%) 87 (34.9%) 2.3. Procedure
Good 180 (37.1%) 102 (41.0%)
Fair 40 (8.2%) 37 (14.9%)
Poor 4 (0.8%) 3 (1.2%) Participants attended a medical assessment during which
Current smoker (n=718) 46 (9.7%) 20 (8.2%) .59 clinical and cognitive measures were obtained. Height and
Body mass index (kg/m2) 26.3±3.7 26.3±4.9 .99 weight were measured, from which body mass index (BMI)
Financial strain (0–100) 6.1±12.0 7.5±13.0 .13 was calculated. The questionnaire containing the socio-
Neighborhood crime (0–3) 0.84±0.56 1.07±0.65 .001
economic and demographic measures, and assessments of
Psychological distress (0–30) 2.20±4.9 3.65±6.4 .001
Social isolation (0–24) 10.8±4.3 10.8±4.2 .94 sleep problems, psychological well-being, financial strain,
Emotional support (0–100) 66.5±19.3 64.5±18.0 .18 neighborhood crime, the GHQ, social isolation, emotional
Negative social interaction (0–100) 19.3±16.9 22.2±19.1 .35 support, and negative social interactions was completed.
Sleep problems (0–100) 25.4±21.9 33.5±24.8 .001 Participants were instructed in the method of affect sampling
Positive affect (0–100) 34.2±38.8 30.7±37.9 .024
which was carried out in the context of salivary cortisol
Eudaimonic well-being 73.8±13.6 71.2±15.4 .019
assessments that are described elsewhere [30] and recorded
their ratings in a booklet along with the timing of measures.
“how often does it happen that you do not have enough These were returned by post.
money to afford the kind of food or clothing that you and
your family should have?” and “how much difficulty do you 2.4. Statistical analysis
have in meeting the payment of bills?”. Each was scored on
five-point scales from no problem to very great problem. The proportion of positive affect measures over the day
Responses were skewed, with 66.8% reporting no problem that were rated as ‘very much’ or ‘extremely’ was computed.
with either item. Responses were therefore categorized into With four ratings, each individual could be categorized as
no financial strain, and some financial strain. Neighborhood being in a positive affective state on 0%, 25%, 50%, 75%, or
crime was measured with three questions concerning the 100% of occasions. Assessments of eudaimonic well-being
likelihood of the participant's home being broken into, being were divided into quintiles to make them comparable with
mugged or robbed, or having their car being broken into or positive affect measures. Men and women were compared on
stolen. Each item was rated on a four-point scale from not at all measures using χ2 tests for categorical and t-tests for
all to very worried. Ratings were averaged and could range continuous variables. The relationships between good sleep
from 0 to 3. The Cronbach α for this scale was 0.81. and positive affect and eudaimonic well-being were analysed
Social isolation was assessed with an adaptation of with separate linear regression analyses on sleep problem
Berkman and Syme's [28] social network measure, with scores. Age, gender, household income, and employment
three items about relatives outside the household and three status were included in the models. Results are presented as
questions about friends or acquaintances. Participants were standardized regression coefficients (β), with negative
asked whether there were any relatives or friends with whom coefficients indicating that positive states are associated
they had regular contact by visit, telephone, email, or letters; with fewer sleep problems. Effects are illustrated by plotting
the frequency of visits; and the number of times per month mean sleep problem scores associated with the five levels of
they saw these relatives or friends. Responses were rated positive affect and the quintiles of eudaimonic well-being,
from almost daily to never/almost never. Summed scores after adjustment for covariates. The associations between
could range from 0 to 24, with higher scores indicating psychosocial risk factors, positive affect, and eudaimonic
greater social isolation (α=0.72). Emotional support and well-being were analysed with separate regressions for each
negative social interactions were assessed using the Close psychosocial factor on these variables, again with age,
Persons Questionnaire [29]. Participants designated the gender, household income, employment status, and self-
single individual to whom they had felt closest over the rated health as covariates. Finally, we determined whether
previous 12 months and rated a series of statements associations between psychosocial risk factors and good
concerning the support provided by this relationship. sleep were modified by positive affect and eudaimonic well-
Eight items contributed to the emotional support scale being by adding these factors to the regression models. The
(e.g., “how much in the past 12 months did this person make change in β associated with adding psychosocial factors to
412 A. Steptoe et al. / Journal of Psychosomatic Research 64 (2008) 409–415
Table 2
Associations between psychosocial risk factors, positive affect, and eudaimonic well-being
Positive affect Eudaimonic well-being
Psychosocial factor Regression coefficient β ⁎ P Regression coefficient β ⁎ P
Financial strain −.032 .40 −.209 .001
Neighborhood crime −.044 .24 −.191 .001
Psychological distress −.129 .001 −.361 .001
Social isolation −.143 .001 −.185 .001
Emotional support .171 .001 .219 .001
Negative social interaction −.074 .047 −.276 .001
⁎ Adjusted for age, gender, household income, employment status, and self-rated health.
A. Steptoe et al. / Journal of Psychosomatic Research 64 (2008) 409–415 413
Table 3
Associations between psychosocial risk factors and sleep problems
Sleep problems controlling for age, gender, Sleep problems additionally controlling for
household income, and self-rated health positive affect and eudaimonic well-being
Psychosocial factor Regression coefficient β P Regression coefficient β P
Financial strain .098 .008 .026 .47
Neighborhood crime .063 .086 −.006 .86
Psychological distress .418 .001 .331 .001
Social isolation .040 .29 −.039 .28
Emotional support −.067 .065 .024 .49
Negative social interaction .222 .001 .134 .001
movement during sleep. Our measure of eudaimonic well- adjustment for self-rated health. But it is notable that the
being included scales pertaining to environmental mastery, strength of all effects was substantially reduced after positive
autonomy, self-acceptance, and purpose in life. affect and eudaimonic well-being were included in the
It is notable that the associations with sleep problems regression models. The regression coefficients for the
were stronger for eudaimonic well-being than positive affect. significant effects were reduced by 20.8–73.5%, suggesting
One explanation may be that the measurement of positive that associations were influenced by positive psychological
affect was less robust, since it was based on ratings over a states. Happier individuals with a greater sense of purpose
single day. Measurement of positive affect over several days and positive orientation may be protected in part from the
with repeated measures might provide a more representative adverse impact of stress and adversity.
index [34]. Alternatively, the broader multidimensional It should be emphasized that this study was cross-
concept of eudaimonic well-being may be more funda- sectional, and no causal influences can be drawn between
mental, so have a stronger association with sleep. positive psychological states and good sleep. It is likely that
There is little evidence relating positive affect with low poor sleep both engenders lower positive affect and reduced
levels of disturbed sleep in adults free of chronic illness or eudaimonic well-being, and that positive psychological
diagnosed sleep disorders. Bardwell et al. [35] found no states lead to better sleep. Intervention research modifying
associations between mood and sleep problems in patients either psychological states or sleep patterns would help
without sleep apnoea, while the Quality of Well-Being scale delineate the relative importance of these two processes.
was not related to sleep variables in a random sample of San This study has a number of limitations. The most
Diego residents [15]. An important feature of the present important is that we had no objective measures of sleep
results is that the association between positive affect and disturbance, and findings were based on self-report. Self-
sleep disturbance was independent of psychological distress. reported sleep problems may be influenced by plaintive set
When we repeated the analyses with the Center for and negative affectivity [39], although it is notable that
Epidemiologic Studies Depression scale as an alternative associations between positive psychological states and good
measure of dysphoria, the relationship between positive sleep remained significant after psychological distress had
affect and good sleep (β=−0.10, P=.003) and between been included as a covariate. These results may not
eudaimonic well-being and good sleep (β=−0.22, P b.001) generalize to individuals with severe levels of affective
remained significant. The link between sleep problems and disturbance and clinical psychiatric conditions. Although the
depression, anxiety, and distress is well documented. Our sample is relatively large, the members of the Whitehall II
findings indicate that even when this is taken into account, cohort are British civil servants, and the results may not
the associations with positive psychological states remain generalize to other populations. Data were collected during
significant, suggesting that positive states are important over the seventh phase of assessment of the cohort, and there has
and above the absence of negative psychological states. been some attrition from the sample since it was first
We sought to establish whether the relationship between recruited in 1985–1988 [23]. More lower than higher SES
positive psychological states and good sleep was indepen- participants have dropped out of the cohort, and this may
dent of factors that potentially influence both. Lower SES have had a differential impact on the characteristics of SES
has been related to greater reports of sleep problems in a groups in this analysis. The CASP-19 does not capture all the
number of studies [17,18], but the correlation was not facets of eudaimonic well-being, missing out the personal
significant in this population. Nevertheless, lower SES was growth and positive relationships domains. The ecological
moderately associated with reduced positive affect and momentary assessment positive affect data were collected
eudaimonic well-being, as previously observed [36,37]. The only on a single day. Nevertheless, the results indicate that
relationships between positive psychological states and sleep self-reported sleep disturbances are associated with reduced
were also independent of self-rated health, age, and gender, positive affect and reduced eudaimonic well-being even after
all of which have been associated with sleep problems in psychological distress, demographic, and medical factors are
previous research [2,3,17]. taken into account, and may partly explain associations
The second aim of this study was to discover whether between poor sleep and psychosocial risk factors. A better
positive psychological states modify the association between understanding of these relationships may benefit individuals
psychosocial risk factors and poor sleep, attenuating the with sleep problems, while extending the health-related
strength of effects. We have previously shown that positive ramifications of positive psychological states.
affect is associated with greater social connectedness and
emotional support [38], and the present analyses indicate Acknowledgments
that eudaimonic well-being is also related to a favorable
psychosocial risk profile (Table 2). As expected, chronic life This study was supported by the Medical Research
stress in the form of financial strain, poor social relationships, Council and the British Heart Foundation. Jane Wardle is
and psychological distress was related to poor sleep (Table 3). supported by Cancer Research UK. The authors acknowl-
The associations of poor sleep with neighborhood crime and edge the assistance of the Whitehall II research team who
lack of emotional support were weaker and did not survive were involved in data collection.
A. Steptoe et al. / Journal of Psychosomatic Research 64 (2008) 409–415 415
References [21] Friedman EM, Hayney MS, Love GD, Urry HL, Rosenkranz MA,
Davidson RJ, Singer BH, Ryff CD. Social relationships, sleep quality,
[1] Kripke DF, Garfinkel L, Wingard DL, Klauber MR, Marler MR. and interleukin-6 in aging women. Proc Natl Acad Sci U S A 2005;
Mortality associated with sleep duration and insomnia. Arch Gen 102:18757–62.
Psychiatry 2002;59:131–6. [22] Steptoe A, Marmot M. Burden of psychosocial adversity and
[2] Strine TW, Chapman DP. Associations of frequent sleep insufficiency vulnerability in middle age: associations with biobehavioral risk
with health-related quality of life and health behaviors. Sleep Med factors and quality of life. Psychosom Med 2003;65:1029–37.
2005;6:23–7. [23] Marmot M, Brunner E. Cohort profile: the Whitehall II study. Int J
[3] Foley D, Ancoli-Israel S, Britz P, Walsh J. Sleep disturbances and Epidemiol 2005;34:251–6.
chronic disease in older adults: results of the 2003 National Sleep [24] Shamaei-Tousi A, Steptoe A, O'Donnell K, Palmen J, Stephens JW,
Foundation Sleep in America Survey. J Psychosom Res 2004;56: Hurel SJ, Marmot M, Homer K, D'Aiuto F, Coates ARM, Humphries
497–502. SE, Henderson B. Plasma heat shock protein 60 and cardiovascular
[4] Roberts RE, Shema SJ, Kaplan GA, Strawbridge WJ. Sleep complaints disease risk: the role of psychosocial, genetic and biological factors.
and depression in an aging cohort: a prospective perspective. Am J Cell Stress Chaperones 2007;12:384–92.
Psychiatry 2000;157:81–8. [25] Jenkins CD, Stanton BA, Niemcryk SJ, Rose RM. A scale for the
[5] Tugade MM, Fredrickson BL. Resilient individuals use positive estimation of sleep problems in clinical research. J Clin Epidemiol
emotions to bounce back from negative emotional experiences. J Pers 1988;41:313–21.
Soc Psychol 2004;86:320–33. [26] Moul DE, Hall M, Pilkonis PA, Buysse DJ. Self-report measures of
[6] Steptoe A, Wardle J, Marmot M. Positive affect and health-related insomnia in adults: rationales, choices, and needs. Sleep Med Rev
neuroendocrine, cardiovascular, and inflammatory processes. Proc 2004;8:177–98.
Natl Acad Sci U S A 2005;102:6508–12. [27] Hyde M, Wiggins RD, Higgs P, Blane DB. A measure of quality of life
[7] Marsland AL, Cohen S, Rabin BS, Manuck SB. Trait positive affect in early old age: the theory, development and properties of a needs
and antibody response to hepatitis B vaccination. Brain Behav Immun satisfaction model (CASP-19). Aging Ment Health 2003;7:186–94.
2006;20:261–9. [28] Berkman LF, Syme SL. Social networks, host resistance, and mortality:
[8] Blazer DG, Hybels CF. What symptoms of depression predict mortality a nine-year follow-up study of Alameda County residents. Am J
in community-dwelling elders? J Am Geriatr Soc 2004;52:2052–6. Epidemiol 1979;109:186–204.
[9] Ostir GV, Markides KS, Peek MK, Goodwin JS. The association [29] Stansfeld S, Marmot M. Deriving a survey measure of social support:
between emotional well-being and the incidence of stroke in older the reliability and validity of the Close Persons Questionnaire. Soc Sci
adults. Psychosom Med 2001;63:210–5. Med 1992;35:1027–35.
[10] Pressman SD, Cohen S. Does positive affect influence health? Psychol [30] Steptoe A, O'Donnell K, Badrick E, Kumari M, Marmot MG.
Bull 2005;131:925–71. Neuroendocrine and inflammatory factors associated with positive
[11] Ryff CD, Keyes CL. The structure of psychological well-being affect in healthy men and women: Whitehall II study. Am J Epidemiol
revisited. J Pers Soc Psychol 1995;69:719–27. 2008;167:96–108.
[12] Ryan RM, Deci EL. On happiness and human potentials: a review of [31] Kraemer HC, Stice E, Kazdin A, Offord D, Kupfer D. How do risk
research on hedonic and eudaimonic well-being. Annu Rev Psychol factors work together? Mediators, moderators, and independent,
2001;52:141–66. overlapping, and proxy risk factors. Am J Psychiatry 2001;158:
[13] Moore P, Bardwell WA, Ancoli-Israel S, Dimsdale JE. Association 848–56.
between polysomnographic sleep measures and health-related [32] Kahneman D, Krueger AB. Developments in the measurement of
quality of life in obstructive sleep apnea. J Sleep Res 2001;10: subjective well-being. J Econ Perspect 2006;20:3–24.
303–8. [33] Schwarz N. Self-reports: how the questions shape the answers.
[14] Fosse R, Stickgold R, Hobson JA. Emotional experience during rapid- Am Psychol 1999;54:93–105.
eye-movement sleep in narcolepsy. Sleep 2002;25:724–32. [34] Polk DE, Cohen S, Doyle WJ, Skoner DP, Kirschbaum C. State and
[15] Jean-Louis G, Kripke DF, Ancoli-Israel S. Sleep and quality of well- trait affect as predictors of salivary cortisol in healthy adults.
being. Sleep 2000;23:1115–21. Psychoneuroendocrinology 2005;30:261–72.
[16] Ryff CD, Singer BH, Dienberg Love G. Positive health: connecting [35] Bardwell WA, Berry CC, Ancoli-Israel S, Dimsdale JE. Psychological
well-being with biology. Philos Trans R Soc Lond B Biol Sci 2004; correlates of sleep apnea. J Psychosom Res 1999;47:583–96.
359:1383–94. [36] Marmot MG, Fuhrer R, Ettner SL, Marks NF, Bumpass LL, Ryff CD.
[17] Moore PJ, Adler NE, Williams DR, Jackson JS. Socioeconomic status Contribution of psychosocial factors to socioeconomic differences in
and health: the role of sleep. Psychosom Med 2002;64:337–44. health. Milbank Q 1998;76:403-48, 305.
[18] Gellis LA, Lichstein KL, Scarinci IC, Durrence HH, Taylor DJ, [37] Subramanian SV, Kim D, Kawachi I. Covariation in the socio-
Bush AJ, Riedel BW. Socioeconomic status and insomnia. J Abnorm economic determinants of self rated health and happiness: a
Psychol 2005;114:111–8. multivariate multilevel analysis of individuals and communities in
[19] Sekine M, Chandola T, Martikainen P, Marmot M, Kagamimori S. the USA. J Epidemiol Community Health 2005;59:664–9.
Work and family characteristics as determinants of socioeconomic and [38] Steptoe A, O'Donnell C, Marmot MG, Wardle J. Positive affect and
sex inequalities in sleep: The Japanese Civil Servants Study. Sleep psychosocial processes related to health. Br J Psychol 2007 June 27;
2006;29:206–16. [Epub ahead of print].
[20] Akerstedt T, Knutsson A, Westerholm P, Theorell T, Alfredsson L, [39] Watson D, Pennebaker JW. Health complaints, stress, and distress:
Kecklund G. Sleep disturbances, work stress and work hours: a cross- exploring the central role of negative affectivity. Psychol Rev 1989;96:
sectional study. J Psychosom Res 2002;53:741–8. 234–54.