We Know Bioprocessing

Fermentors and bioreactors for research through production

»Bioprocessing is at the heart of
many industries and Eppendorf
has the right solutions for all of
them.«
 3

Perfect Mix

Joint expertise for excellent solutions
Already well established as a global leader for liquid-, cell- and
sample- handling products, Eppendorf entered the bioprocess
market in 2007 through the acquisition of New Brunswick™
Scientific. This strategic move not only added 70 years of New
Brunswick experience with autoclavable and sterilizable-in-place
fermentors and bioreactors, it also allowed Eppendorf to add an
array of lab products such as shakers, freezers, and incubators to
its already comprehensive portfolio of products.
In 2012, Eppendorf further expanded its bioprocess
expertise and product offerings by acquiring DASGIP®, a
pioneer in the development and production of benchtop and
small scale parallel bioreactors, and information technology
(IT) systems. Ideally-suited for high throughput and process
development work, the DASGIP portfolio further expanded
the Eppendorf bioprocess portfolio.
With a comprehensive offering of single-use and traditional
products for the growth of mammalian, microbial, insect, plant
and algae cells, and working volumes of 60 mL – 2,400 L, the
Eppendorf bioprocess portfolio can satisfy the demands of
process development through production.

Scalable systems Single-use options Software solutions

> Eppendorf bioprocess systems
cover a wide range of working
volumes for applications from
research through production
> Innovative single-use vessels
feature traditional stirred tank
design
> Comprehensive software
packages offer innovative
solutions for advanced process
control and information
management
4

A New Scale of Bioprocessing

»Solutions that grow with you.«

From the parallel mini bioreactor system for early stage bioprocess development, the benchtop and parallel bioreactor
systems for the laboratory scale to the sterilize-in-place solutions for production: Eppendorf offers users from industry and
research extensive bioprocess solutions from a single source and meets the highest quality demands.

Mini bioreactor systems Parallel benchtop systems

60 - 250 mL 0.2 L - 2 L 0.7 - 3.8 L

Small scale Bench scale

 5

Benchtop bioreactors and fermentors SIP bioreactors and fermentors

0.4 - 10 L 1.3 - 40 L 5.5 - 2,400 L

Pilot scale Production scale

6

DASbox® Small Scale Bench Scale

Mini Bioreactor Parallel Bioreactor Parallel Bioreactor BioFlo® /
System Systems Systems CelliGen BLU
®
CelliGen 115

Working volume range1

60 - 250 mL 0.2 - 2 L 0.7 - 3.8 L 1.3 - 40 L 0.4 - 10.5 L

Single-use vessels available

• • • • •
Glass vessels, autoclavable
• • • •
Stainless-steel vessels, SIP

Interchangeable vessels
• • • • •
Bacteria/yeasts/fungi
• • • •
Plant cells/algae
• • • •
Mammalian/animal cells
• • • • •
Stem cells
• • • • •
Insect cells
• • • • •
Number of parallel units
Up to 24 Up to 16 Up to 16 Up to 3

Controller2
DWC DWC DWC RPC RPC

Touchscreen controller
• •
BioCommand ®

• •
DASware®
• • • • •
Gas mixing options 4 gas 4 gas 4 gas 4 gas 4 gas
(air, N2, O2, CO2) (air, N2, O2, CO2) (air, N2, O2, CO2) (air, N2, O2, CO2) (air, N2, O2, CO2)
Gas flow control3
TMFC R or TMFC R or TMFC R4 or TMFC R or TMFC

Exhaust analysis
• • • • •
Optical density measurement
• • • •
Validation
•
1
Realized using multiple vessels
2
Controllers: DWC=DASware control, RPC=Reactor Process Controller, BCS=BioFlo Control Software, PLC=Programmable Logic Controller
3
Gas Flow Controllers: R=Rotameter, TMFC=Thermal Mass Flow Controller
4
Rotameter is for overlay only
 7

BioFlo 320 BioFlo 415 BioFlo 510 CelliGen 510 BioFlo 610 BioFlo Pro CelliGen Pro

0.25 - 40 L 2.0 -15.5 L 5.5 - 32 L 5.5 - 32 L 13 - 100 L 32 - 2400 L 18.8 - 520 L

•
•
• • • • • •
• •
• • • • •
• • • • • • •
• • •
• • •
• • • •
Up to 8

BCS RPC RPC/PLC RPC/PLC RPC PLC PLC

• • • • • • •
• • • • • • •
• •
4 gas 4 gas 4 gas 4 gas 2 gas 2 gas 4 gas
(air, N2, O2, CO2) (air, N2, O2, CO2) (air, N2, O2, CO2) (air, N2, O2, CO2) (air, O2) (air, O2) (air, N2, O2, CO2)

TMFC R or TMFC R or TMFC R or TMFC R or TMFC R or TMFC R or TMFC

• • • • • • •
•
• • • • • • •
8
Parallel Bioprocessing for Unparalleled Results
DASGIP® Bioreactor Systems
DASbox
Parallel mini bioreactor system
60 - 250 mL working volume
> 4-fold parallel system extendable to up to 24 parallel
operated glass or single-use bioreactors
> Suitable for cell culture and microbial fermentation
> Compact mini bioreactor system: requires only 7 cm
(3 inches) bench space per bioreactor
> Optimal tool for DoE and scale down approaches
> Agitation control supporting overhead-driven Rushton,
marine-type or pitched blade impellers
> Innovative liquid-free temperature control system needs
no coolant agent supply and supports independent
temperature control for each bioreactor
> Accurate monitoring and control of pH, DO and level
> Variable speed pumps for accurate liquid addition and
operation in batch, fed-batch, continuous and cyclic
perfusion mode
> 4 mass flow controllers per bioreactor allow for individual
mixing of air, N2, O2 and CO2 to headspace and/or
submerged
> Novel liquid-free Peltier exhaust condenser with easy to
handle slide in - slide out activation and deactivation
> DASware control Software for advanced process control
> Compatible with DASware® Software Suite for
interconnectivity and bioprocess information management
DASGIP Parallel Bioreactor Systems
for small-scale applications
0.2 - 2 L working volume
> Parallel operation of up to 16 glass or single-use
bioreactors
> DASGIP Bioblock for advanced and user-friendly
temperature control
> Suitable for cell culture, microbial fermentation and
phototrophic cultivation
> Modular design of control units allows for flexible
system configurations that meet the demands of specific
applications
> Control of agitation, pH, level and DO (including
customizable cascade modes) in each bioreactor
> Variable speed pumps for accurate liquid addition and
operation in batch, fed-batch, continuous and cyclic
perfusion mode
> Optical absorbance measurement for online calculation of
e.g. OD600 or cell dry weight
> TMFC individual gas mixing of air, N2, O2 and CO2
> Online calculation of OTR, CTR and RQ
> DASware control Software for advanced process control
> Compatible with DASware Software Suite for
interconnectivity and bioprocess information management
 9

Choice of impellers

DASGIP PBR4
for phototropic cultivation

DASGIP Parallel Bioreactor Systems

for bench-scale applications
0.7 - 3.8 L working volume

> Parallel operation of up to 16 glass or single-use

DASGIP Peltier Exhaust Condensors
bioreactors
for selected glass and single-use vessels
> Suitable for cell culture, microbial fermentation and
phototrophic cultivation
> Modular design of control units for flexible system
configurations that meet the demands of specific
applications
> Temperature control via heating blankets and cooling
fingers
> Control of agitation, pH, level and DO (including
customizable cascade modes) in each bioreactor
> Variable speed pumps for accurate liquid addition and DASGIP Compression Fittings
operation in batch, fed-batch, continuous and cyclic for headplate ports
perfusion mode
> Optical absorbance measurement for online calculation of
e.g. OD600 or cell dry weight
> TMFC individual gas mixing of air, N2, O2 and CO2
> Online calculation of OTR, CTR and RQ
> DASware control Software for advanced process control
> Compatible with DASware Software Suite for
interconnectivity and bioprocess information management
10
Exceedingly Versatile —
Bench Scale Fermentors and Bioreactors
New Brunswick CelliGen BLU
Bench-scale single-use bioreactor
1.3 - 40 L working volume
> Designed for high-density cell culture in research or
production applications
> Rigid-walled, single-use stirred-tank vessel for proven
performance and scalability
> Eliminates vessel autoclaving and cleaning
> All vessel components in product contact are made of
USP Class VI materials and tested for leachables and
extractables
> Packed-bed vessel: first single-use vessel to feature
proprietary New Brunswick Fibra-Cel® and packed-
bed impeller
> Features intuitive New Brunswick RPC control
software and touchscreen interface
> Non-invasive pH and DO technology
> Validation packages available
> Seven additional analog input/output connections for
local integration of ancillary equipment
> Compatible with DASware Software Suite
or BioCommand for bioprocess information
management
New Brunswick BioFlo/CelliGen 115
Bench-scale fermentor/bioreactor
0.4 - 10.5 L working volume
> Precise temperature control provided through heat
blanketed vessel (shown above) or water jacketed
vessel
> Compatible BioBLU single-use vessels
> Pre-programmed with both fermentation and cell
culture operating modes for flexibility
> Ability to control up to three independent reactors
from a single easy-to-use touchscreen
> Features intuitive New Brunswick RPC control
software and touchscreen interface
> Eight interchangeable glass vessel options
> Customizable gas flow and mixing options
> Available in pre-defined packages for easy ordering,
or fully configurable systems to meet specific needs
> Compatible with DASware Software Suite
or BioCommand for bioprocess information
management

BioFlo 320
Bench-scale fermentor and bioreactor
0.6 - 10.5 L working volume (autoclavable)
0.25 - 40 L working volume (single-use)
> Interchangeable autoclavable and BioBLU Single-Use
Vessels
> Integrated Mettler Toledo® Intelligent Sensor
Management (ISM) platform
> Control up to eight systems from a single-user
interface
> Universal control for both microbial and cell culture
applications
> Field-upgradable TMFC drawers for sparge and
overlay gas
> Enhanced software package with new cascade and
time profile features
> Built-in optical pH sensing technology for use with
the BioBLU Single-Use Vessels
> Ethernet communication for multi-unit control,
Eppendorf SCADA software, and IP addressing
> Up to six integrated pumps capable of operating in
variable speed mode
> Eight independently controlled process gas supplies
> Suitable for batch, fed batch, and continuous
processes
> Validation packages available for GMP regulated
processes
11
New Brunswick BioFlo 415
Bench-scale fermentor
2.0 - 15.5 L working volume
> Automated SIP process utilizes only a water supply
and the system’s built-in electric heat blanket for
sterilization - no external steam supply needed
> Eliminates the need for transferring vessels to the
autoclave for sterilization
> Features intuitive New Brunswick RPC control
software and touchscreen interface
> Fully customizable trend screen for data logging
> User-definable cascades for localized process control
> Validation packages available
> 7 additional analog input/output connections for local
integration of ancillary equipment
> ASME-rated pressure vessel
> Compatible with DASware Software Suite
or BioCommand for bioprocess information
management
12
Industry Standards meet Flexibility —
Sterilizable-in-Place Fermentors and Bioreactors
New Brunswick BioFlo 510 & CelliGen 510
Bench- and pilot-scale SIP fermentor and bioreactor
5.5 - 32 L working volume
> SIP bench and pilot scale reactors designed to meet
the needs of R&D through production
> Modular design allows for complete system flexibility
> Add or remove components pre- or post-delivery
> Multiple impeller and gas flow options
> Features intuitive New Brunswick RPC control
software and touchscreen interface
> Optional Allen Bradley® PLC
> Integrated load cell for online vessel volume
monitoring
> Fully automated SIP sequence for sterilization
> Validation packages available
> 7 additional analog input/output connections for local
integration of ancillary equipment
> ASME-rated pressure vessel
New Brunswick BioFlo 610
Pilot-scale SIP fermentor
13 - 100 L working volume
> Integrated mobile skid simplifies transport
> Pilot production system without the traditional cost or
footprint
> Modular design allows for complete system flexibility
> Features intuitive New Brunswick RPC control
software and touchscreen interface
> Fully integrated load cell for online vessel volume
monitoring
> Validation packages available
> 7 additional analog input/output connections for local
integration of ancillary equipment
> ASME-rated pressure vessel
 13

1 2 3 4 5

Eppendorf offers a diverse range of specialized impellers for

New Brunswick bioreactors including:
1. Spin Filter (shown with marine blade impeller)
2. Cell-Lift Impeller
3. Packed-Bed Basket Impeller with draft tube for use with
Fibra-Cel® disks (complete impeller assembly shown
New Brunswick BioFlo Pro & CelliGen Pro below)
Large-scale SIP fermentor and bioreactor 4. Pitched Blade Impeller
BioFlo Pro: 32 - 2,400 L working volume 5. Marine Blade Impeller
CelliGen Pro: 18.8 - 520 L working volume Rushton and Rushton-type Impellers (not shown)
> Large scale industrial system with full adherence to
cGMP and GAMP® guidelines
> Modular design with over a hundred options allows
for high level of customization pre and post delivery Impellers and Fibra-Cel disk
> Multiple validation package offerings options enhance growth and
> Open piping skid allows for easy accessibility productivity
> NEMA-4 (IP65) rated control cabinet with industry-
standard Allen Bradley PLC
> Flush mounted vessel ports for enhanced vessel
design and drainability
> Multiple impeller and gas flow options
> Optional validation packages available
> ASME-rated pressure vessel

Fibra-Cel disks are a solid support material used with

New Brunswick packed-bed bioreactors for growth of
adherent mammalian, animal and insect cells, when secreted
proteins are desired. Key advantages:
> Cell densities averaging 10 times higher than traditional
microcarriers, due to protection from shear forces
> Higher mass transfer of nutrients and oxygen
> Low pressure drop across the bed for efficient scale-up
> Higher surface-to-volume ratios
14
BioBLU® Single-Use Vessels —
Dependability through Proven Design
From 65 mL to 40 L, working volume, Eppendorf has a single-use solution to meet your needs
Single-use simplicity
With renowned polymer expertise, Eppendorf is proud to
offer the largest portfolio of rigid walled stirred-tank single-
use vessels – in small and bench scale, to be operated
with parallel DASbox Mini Bioreactor System, DASGIP
Parallel Bioreactor Systems, or New Brunswick bench scale
systems. Kits are also available for third-party bioprocess
systems. They are easy to install and compatible with
popular Applikon® and Sartorius® autoclavable bioprocessing
systems.
Benefits
> Proven performance and scalability of stirred-tank design
> User-friendly set-up for rapid turnaround, shorter
development times and lower operating costs
> Specifically adapted designs available for microbial
applications
> Packed-bed configuration with Fibra-Cel disks is ideal for
perfusion/continuous processes
> USP Class VI and animal component-free materials,
making vessels appropriate for GMP production
> Non-invasive or industry standard sensor technology

Single-use for DASbox. Choice of
BioBLU 0.3 mini vessels for cell culture and
microbial applications. With small working
volumes from 65 to 250 mL perfectly suited
for DoE appoaches.
Single-use for DASGIP Parallel Bioreactor
Systems. Parallel processing with
BioBLU 5c and BioBLU 14c vessels for best
scalability with processes developed in
DASbox or DASGIP Bioblock.
Single-use for DASGIP Bioblock. Choice
of BioBLU 1 vessels for cell culture and
microbial application, designed for truly
parallel bioprocessing. Working volume
range from 0.25 to 1.25 L.
BioBLU 5p vessel for adherent cells and
perfusion processes. Packed-bed vessels
utilizing the proprietary Eppendorf packed-
bed impeller design with pre-loaded Fibra-
Cel disks.
15
Single-use for Eppendorf and third-
party bioreactor controller. BioBLU 5c,
BioBLU 14c and BioBLU 50c for best
scalability in cell culture. Working volume
range from 1.3 to 40 L.
BioBLU 0.3f and BioBLU 1f vessels fully
address the specific needs of fermentation,
including mass and heat transfer. Working
volume range of 65 mL to 1.25 L.
16

Eppendorf Bioprocess Software —

Much More Than Just Bioprocess Control

Eppendorf bioprocess software solutions - advanced monitoring and control through comprehensive information management

Eppendorf offers BioCommand® and DASware control Track & Batch Batch DASware
Supervisory Control and Data Acquisition (SCADA) software Trend Control Control control
packages for advanced bioprocess control. Next-generation Features Plus
bioprocess management is provided by the comprehensive Batch info • • • •
DASware software suite. User-defined event log • • • •
Summary • • • •
Loop info • • • •
BioCommand software
Alarms • • • •
Enhances your ability to monitor, control and log data from
Trend graphs • • • •
your fermentation and cell culture processes through your
Data log • • • •
personal computer. Three distinct packages provide the tools Reports • • • •
needed for research, optimization, and if needed, the security Graphical programming • •
and audit trails to meet your regulatory requirements. Script programming • • •
> Track and Trend: Provides the ability to trend and control Synoptic display • • •
parameter setpoints, establish alarm settings, and produce Equipment lock-out • • •
batch records; and is ideal for basic process management Audit trail/security • •
> Batch Control: Additional enhanced control features 21 CFR Part 11 • (•)*
including a sophisticated programming module, custom OPC compliance • • • •
synoptic display window, and equipment lock-out feature Parallel calibration •
> Batch Control Plus: Includes three levels of security, event DASware compatibility •
logs, and audit-trail capabilities to be compatible with the * 21 CFR Part 11 compatibility is available for DASware control as an
optional package. Please contact us for further information.
FDA 21 CFR Part 11
 17

DASware control software

Is at the core of all DASbox and DASGIP systems and was
designed for parallel cultivation with individual control of
each bioreactor. Parallel calibration and cleaning procedures
are supported as well as integrated batch functionality for
process and recipe management. It features easy one-click
start, configurable online charts, trending, logging rates
and online configurable control parameters and profiles.
Full controller scripting eases scale-up and scale-down.
Automated data export, reporting and chart creation with
Microsoft® Excel® add-in as well as professional database
with managed access (Microsoft® SQL Server®) support
advanced reporting and data processing. DASware analyze
and DASware connect enable OPC client/server functionality.

DASware® software suite

Next-generation bioprocess management
A suite of smart and flexible software solutions to accelerate bioprocess development. It enables interconnectivity of
bioreactors with external lab-devices, comprehensive data- and information management, Design of Experiments (DoE)
and remote control of bioprocesses. DASware can be used with any Eppendorf benchtop bioreactor solution as well as
third-party bioreactor controllers.

DASware access DASware design

> Remote monitoring and control of > Applies the Design of Experiments
bioprocesses via PC, Notebook and (DoE) concept via a full factorial DoE
Netbook or with the DASGIP iApp via builder or by importing DoE designs
iPhone®, iPod touch® and iPad® from third-party DoE tools

DASware analyze DASware discover

> Seamless integration of external lab > A comprehensive and user-friendly
devices to the bioreactor allows for information management solution for
process automation and feedback bioprocessing
control loops

DASware connect DASware migrate

> Integration into process control
systems and legacy corporate
historians facilitating company-wide
access to all relevant bioprocess data
> Integration of various bioreactor
control units and systems - this
software is the gateway to
comprehensive functionality of
DASware on third-party bioreactor
controllers
18

Eppendorf Life Science Solutions

PCR cyclers Electroporation & microinjection Centrifugation

Amplify target DNA with premium, Comfortable manipulation of microbial, Outstanding ergonomic operation,
reliable and reproducible results animal and human cells superior temperature control and
outstanding product quality

CO2 incubators Shakers Ultra-low temperature freezers

Support cGMP work, stem cell Provide researchers with an easy-to- Safe and energy efficient sample
research, cancer research, as well as use system for culturing cells built on a storage at -86 °C
traditional cell culture history of dependability
 19

Perfect Your Workflows

Eppendorf — Your workflow oriented system supplier Workflows
As a workflow oriented provider of lab equipment, Eppendorf offers Antibody Production
instruments, consumables and accessories that perfectly fit your Biochemistry
processes in the lab and thus your daily lab work. Our comprehensive Biofuels
solutions are engineered with smart innovations to simplify or even Bioprocessing R&D
eliminate cumbersome lab work. You will find flexible solutions to Cell Biology
prepare, handle, and analyze your samples and cells. Microbiology
Molecular Biology
Next Generation Sequencing
Stem Cells

>>Further workflows are available on www.eppendorf.com/workflows

 ®
In touch with life

Your local distributor: www.eppendorf.com/contact

Eppendorf AG · 22331 Hamburg · Germany
eppendorf@eppendorf.com • www.eppendorf.com

www.eppendorf.com
Allen Bradley® is a registered trademark of Rockwell Automation, Inc., USA. Mettler Toledo® is a registered trademark of Mettler-Toledo AG, Switzerland. Applikon® is a registered trademark
of Applikon, B.V. Corporation, Netherlands. iPhone®, iPad®, and iPod touch® are registered trademarks of Apple Inc., USA. GAMP® is a registered trademark of the International Society for
Pharmaceutical Engineering, USA. Microsoft®, Excel®, and SQL Server® are a registered trademarks of Microsoft Corporation, USA. Sartorious® is a registered trademark of Sartorius AG,
Germany. Fibra-Cel® is a registered trademark owned by Minerals California, Inc., USA and licensed to Eppendorf, Inc., USA. Eppendorf®, the Eppendorf logo, BioBLU®, and In touch with
life® are registered trademarks of Eppendorf AG, Germany. New Brunswick™ is a trademark of Eppendorf AG, Germany. BioFlo®, CelliGen®, and BioCommand® are registered trademarks of
Eppendorf, Inc., Enfield, Connecticut (USA). DASGIP®, DASbox®, and DASware® are registered trademarks of DASGIP Information and Process Technology GmbH, Germany.
U.S. Design Patents are listed on www.eppendorf.com/ip.
All rights reserved, including graphics and photos. Copyright © 2015 by Eppendorf AG
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