BIOCHEMISTRY ASSISGNMENT [SIDEBAR TITLE]

Submitted by:
TALHA BIN KASHIF
QUESTIONS : ROLL NO 285
1-Name the class and subclass of enzymes required for Dated:
post translational modification of proline and lysine during 5th June 2020
collagen synthesis.
2-What the role of ascorbate and iron in these reactions?
3-How is dehydroascorbate reduced again to be reused?

POSTTRANSLATIONAL MODIFICATION OF PROLINE AND LYSINE:

The posttranslational modification requires the hydroxylation of proline and lysine residues
during the process of collagen formation.This happens at the “Pro alpha chain” stage of
collagen formation

1-ENZYMES INVOLVED:
The enzymes involved in the hydroxylation process of proline and lysine are “PROLYL
HYDROXYLASE” and “LYSYL HYDROXYLASE” respectively.With the EC numbers ie
Prolylhydroxylase : EC 1.14.11.2
Lysyl hydroxylase: EC 1.14.11.4
From these EC numbers we can conclude that the enzymes belong to
CLASS 1 ie OXIDOREDUCTASES and SUBCLASS 14 ie OXYGENASES

2-ROLE OF ASCORBATE AND IRON:

During the posttranslational hydroxylation of proline and lysine
-Ferrous iron is (+2) is oxidized to form ferric ions (+3)
-Ascorbate acts as a reducing agent for the ferric iron and converts it back to ferrous form
,while itself it oxidizes in the redox reaction to form DEHYDROXYASCORBATE (DHA). This
reaction allows it to be reversibly reduced also hence making it reuseable.
-alpha keto glutarate converts into succinate

succinate
Alpha keto
glutarate

PROLINE/LYSINE HYDROXYPROLINE/HY
PROLYL HYDROXYLASE/LYSYL HYDROXYLASE
DROXYLYSINE

OXIDATION

Fe (+2) Fe (+3)

REDUCTION

OXIDATION

DHA

3-REUSE OF D-H-A :
Dehydroxyascorbate (oxidized form) can be reduced to ascorbate by the addition of H-atoms in
a Glutathione Dependent reaction (GSH) by the action of Dehydroxyascorbate Reductase
enzyme (DHAR)

ASCORBATE
QUESTION NUMBER 2 :

Describe the role of vitamin C in giving conformation stability and

strength to collagen fibers. Justify your answers by showing the
biochemical reactions involved.

Vitamin C or ascorbic acid is a coenzyme for the hydroxylase enzymes that catalyse the
hydroxylation of proline and lysine residues.The ascorbic acid allows greater inter-chain
hydrogen bonding between the collagen fibers and play a vital role in maintaining a stable
triple helix structure of collagen.