Serpentina Plant (Rauvolfia serpentina)

as an alternative medicine plant

for abdominal pain

Group 3

Janine Gado

Kizza Peradilla

Harvey Marasigan

Aaron Salayo

Asher Romano Jamilano

Jude Amandy
I. INTRODUCTION

Rauvolfia serpintina, formerly known as serpintina is a safe and effective treatment plant

for abdominal pain. The plant was used by many physicians throughout India and in the 1940s

and then was used throughout the world in the 1950s, including in the United States and Canada.

This small shrub, native to the Orient from India to Sumatra, has for centuries been used in

Indian medicine. In 1952 reserpine, one the several alkaloids in the plant, was isolated from its

root and has since been evaluated in western medicine as one of the most valuable drugs for

treating high blood pressure.

The documentation and phytochemical screening of medicine plants has been an

important way over the years for the discovery of new drugs and pharmaceutical products.

Serpintina has many uses that could benefit our health. The said plant has very low toxicity and

no over dosage cases have been reported. Perhaps it is because unlike any other herbs, you won't

wish to have more recommended dosage due to its bitterness.

BACKGROUND OF THE STUDY

The Rauvolfia serpintina, formerly known as serpintina is a safe and effective treatment

plant for abdominal pain. The plant was used by many physicians throughout India in the 1940s

and then was used throughout the world in the 1950s. The researchers have thought it could be

the researchers main ingredient. The researchers only need to find out what to do the serpintina

plant, then the researchers come up with an idea to make it to a medicine tea.
STATEMENT OF THE PROBLEM

The main objective of this study aims to determine the feasibility of serpintina plant to

cure students who are experiencing abdominal pain.

1. What are the effects of the researchers? is there any property of the plant that are

dangerous?

2. Is the product effective?

3. Is the material in making the product expensive?

HYPOTHESIS

Alternative

The serpintina plant is effective for abdominal pain especially to students who are 13-14

years old.

Null

Some studies have shown that the side effects of serpintina plant can range from mild to

serious and include nasal congestion, it is also not advisable for pregnant woman that may cause

abortion.

SIGNIFICANCE OF THE STUDY

This study aims to determine the effectiveness of serpintina plant as an alternative

medicine plant for abdominal pain, since this study deals with the assessment of individuals,

most especially for students who are suffering from abdominal pain. The researchers would

likely want to know the benefits and effects of individuals who will use their serpintina plants as

an alternative medicine plant for abdominal pain.

SCOPE AND LIMITATION

The scope of the research study was to produce an alternative medicine tea for abdominal

pain, it also included the analysis of its effectiveness based on the experiment made.

RELATED STUDIES AND RELATED LITERATURE

Rauwolfia serpentina is an important medicinal plant belongs to the family Apocynaceae.

This plant is native to Bangladesh, India and other humid region of the world (Roy et al., 1994).

R. serpentina contains some 50 indole alkaloids and most of the total alkaloid content percent

mainly in root back (Klyushnichenko et al., 1995). Cultivation of Rauwolfia in India was

reported by many authors (Biswas, 1956; Dutta et al., 1963; Badhwar et al., 1955). Rauwolfia

serpentina L. Benth. Ex Kurz. Is an evergreen perrenial shrub which grows up to 60m of height.

The family includes 50 species, distributed worldwide in the region of the Himalayas, Indian

Peninsula, Burma, Indonesia and Sri Lanka and is indigenous to India, Bangladesh and other

regions of Asia (Ghani et al., 1998). It is also commonly known as Sarpagadha, Chandrabagha,

Snake root plant, Chotachand, Chandrika and Harkaya etc. (Mallick et al., 2012).

For centuries, root of Rauwolfia serpentina has been used in the traditional unani and

ayurvedic medicine (Andrew and Chevallier 1996). Rauwolfia serpentina contains a variety of

compounds with antioxidant capacity and other health benefits like treatment of diabetes,

cardiovascular disease, cancer and hypertension. The antibacterial and antifungal activities were

high in petroleum ether and acetone extract of Rauwolfia serpentina (Harisananraj et al., 2009).

Rauwolfia serpentina has long being used in India for the treatment of snakebites, hypertension,

high blood pressure and mental illness. Different ethnic groups use this plant to treat snake,

insect and animal bite, mental illness, schizophrenia, hypertension, blood pressure,
gastrointestinal diseases, circulatory disorders, pneumonia, fever, malaria, asthma, skin disease,

scabies, eye diseases, spleen diseases, AIDS, rheumatism, body pain, veterinary diseases etc. this

plant is also being used to prepare fermented food products (Dey and Den, 2010). It has been

stated that this plant is used as antidote against snakebite (Khyade et al., 2011). Plant derived

indole inhibitors were identified from the extracts of Rauwolfia serpentina which has the

capacity to acts as aldose reductase, a potent drug for diabetes (Pathnania et al., 2013). The

methanol root extract of this plant provides the high antioxidant compound phenol of 233mg/gm.

So this plant has significant antibiotic and hypolipidemic activity. (Azmi and Qureshi, 2012).

The phytochemical analysis of Rauwolfia serpentina has many medicinal values. It is used for

the diseases such as Anthelmintic, anti hypertensive, snake bite, diarrhoea, dysentery, cholera,

fever, pneumonia, malaria, body aches, eczema, burns, menstrual disorders, scabies, skin

cancers, asthma, respiratory problems, eye inflammation, spleen diseases and feveopacity of the

cornea and central epilepsy (Rai, 2004; Itoh et al., 2005; Mohanta et al., 2006; Bhattarai et al.,

2009; Dey and Dec, 2010; Azmi et al., 2013; Ghani, 1998). Because of presence of high amount

of alkaloids it is used for the treatment of circulatory disorders (Fabricant and Farnsworth, 2001),

treat liver, abdomen pain, gastrointestinal disorders and to expel intestinal worms form the

children (Nayak et al., 2004; Anisuzzaman et al., 2007; Dey and Den, 2011).

Due to its poor seed germination rate, it cannot be easily increases the number of plants, because

of its high medicinal use, R. serpentina is becoming extinct and is now listed as an endangered

species by the International Union for Conservation of Nature and Natural Resources (IUCN)

(Jain et al., 2003; Singh et al., 2009; Sushila et al, 2013).

Various parts of the Rauwolfia serpentina plant were used as explant and inoculated in

Murashige and Skoog medium containing 2, 4-D and 6-BAP. Maximum callus inductions of
93.65% were obtained in leaf and stem explants. The callus was inoculated in shooting medium

containing BAP and NAA and maximum shoots were obtained. The rooting was induced in

invitro regenerated shoots in MS medium containing IBA and NAA and 100% rooting was

obtained (Panwar et al., 2011).

Various parts of the Rauwolfia serpentina plant were used as explant and inoculated in

Murashige and Skood medium containing 2, 4-D and 6-BAP. Maximum callus induction of

93.65% were obtained in leaf and stem explants. The callus was inoculated in shooting medium

containing BAP and NAA and maximum shoots were obtained. The rooting was introduced in

invitro regenerated shoots in MS medium containing IBA and NAA and 100% rooting was

obtained (Panwar et al., 2011)

CHAPTER III

METHODOLOGY

SUBJECT OF THE STUDY

This study is focused on Serpintina leaves as an alternative medicine

tea for abdominal pain

MATERIALS OF THE STUDY

We know for a fact that our study will center those budgets a bit low.

So we took the initiative to use some materials that wouldn't cost much!

expensive. They are those that can be found in every houses kitchen;

1. 3 grams/ 5-6 Serpintina leaves

2.Drier

3.Blender

4.Small container

PROCEDURE

l.Cut the leaves of from a Serpintina plant.

2.Cut off the leaves from the stem and wash it thorough and dry

3.Dry the leaves for 2-3 day until crisp or just heat it up in a pan.

4.Blend the leaves in a blender or use a mortar and pestle until the leaves are crushed into a

powder substance.
5. And here's the finish product of the Serpintina leaves this can be put in a small container to be

preserved.

DATA GATHERING PROCEDURE

After the alternative medicine tea was produced from the Serpintina plant, the

researchers get its effectiveness by basically mixing the said medicine tea upon the part of the

stomach is in pain.

SAMPLING DESIGN

Researchers use this purposive sampling design since the researchers intends to gather
RESULTS AND FINDINGS

Trials Ingredients

Trials 1 5-6 dry serpintina leaves

Trials 2 10 dry serpintina leaves

Trials 3 10 dry serpintina leaves

RESULTS

1st Trial - The researchers didn't know how many dry serpintina leaves to use. At first they tried

to use 5-6 dry serpintina leaves but it resulted to a failure. In the end, using 10 dry serpintina

leaves was just enough.

2ndTrial -The researchers can't decide if they should boil the plant to extract the nutrients into a

liquid kind of substance or to use mortar and pestle to crush the serpintina into little pieces. The

researchers have chosen to just to crush the serpintina leaves to little piece and to put the

serpintina plant into a zip lock bag.

3rdTrial - The researchers have finally completed all the materials and procedures needed to be

done. The product, serpintina medicine tea, has been successfully created in the end.

Product Day's use Results

Test 1 1-3 day Have not shown any of sign of the

product taking effect

Test 2 3-5 day Have shown slight effect of the
 product
Test 3 6-7 day have shown effect of the product

reducing abdominal pain

RESULT

The product show to have effect in 6-7 days ,the researchers suggest to take the medicine tea

daily to have effect, the researchers found out that the product takes time before it will have any

effect on the user

CONCLUSION

1. The researchers found out that the serpintina plant can be a remedy to abodominal

pain,the serpintina plant is use for many disorder, it can also be use as medicine, research

even said it can be used to fight diabetes. The serpintina plant may have poisonous

property if not careful on using it as an alternative medicine,it is a safe effective treatment

for hypertension.

2. The medicine is effective to remedy the abdominal pain if taken daily, And use after

eating. Making a medicine tea using serpintina plant is not expensive, it's pretty

affordable to make all of the materials use are found in the kitchen.

3. It's easy to make medicine tea using serpintina plant, using materials found in the kitchen

the procedure is can be done easily.

RECOMMENDATION

1. In our investigation the reasearchers found a remedy for abodminal pain, If the research

is use for further investigation,the reasearchers recommend that they should go further

with the research by adding other ingredients that could help to remedy the abdominal

pain.

2. The serpintina plant can be useful for those who have diabetes but it is still a theory no

doctor would suggest this but it could be useful if further investigated.

3. The serpintina plant is use to cure hypertension, some research says that the serpintina

plant may be poisonous,the reasearchers recommend to be careful on reasearching on

serpintina plant.
 ABSTRACT

The study focus on testing if the Serpintina plant can be a medicine tea that cure
abdominal pain study show that Rauwolfia serpentina has alloxan-induced diabetic Wister male
mice. Mice were divided in control (distilled water at 1 mL/kg) and alloxan-induced diabetic
mice which subdivided into diabetic (distilled water at 1 mL/kg), negative (0.05% dimethyl
sulfoxide at 1 mL/kg), positive (glibenclamide at 5 mg/kg) controls, and three test groups (MREt
at 10, 30, and 60 mg/kg). All treatments were given orally for 14 days. Qualitatively MREt
showed the presence of alkaloids, carbohydrates, flavonoids, glycosides, cardiac glycosides,
phlobatannins, resins, saponins, steroids, tannins, and triterpenoids, while quantitatively extract
was rich in total phenols. The flavonoids, saponins and alkaloids were also determined in root
powder. MREt found effective in improving the body weights, glucose and insulin levels,
insulin/glucose ratio, glycosylated and total hemoglobin in test groups as compared to diabetic
control. Similarly, significantly decreased levels of total cholesterol, triglycerides, low-density
lipoprotein (LDL-c), and very low-density lipoprotein (VLDL-c) cholesterols were found in test
groups. Significant lipolysis with improved glycogenesis was also found in liver tissues of all
test groups. ALT levels were found normal in all groups. Thus, MREt improves the glycemic,
antiatherogenic, coronary risk, and cardioprotective indices in alloxan-induced diabetic
mice.Serpintina plant can be used to treat abdominal pain according to some studies.The
researchers have successfully completed the product and already tested it.