LIPASE KIT (Turbidimetrc U.VMethod) Forte cataminson of pase acy Seu, (For nvr dagoste vs on Summary: Lpasesaresnzymes wih hyeolyse gyeatletroiong tfaln fay a. The arzyme and fs cxfacr,colpese Is Frofuced i the pancreas, pase tog abso sexed in Smal arounls by the calvary glands 2 wal as by the sti, pulmonary an resin moose le acs and Eoipase form moar eoolexes wit ne is and ind tase onthe estat wal terae,Delomnaton of Lpase's usc for ivesigason of pencreatc sor. n scale pancreas te pase concenteans 188 2-50 fod the upereloarce it wih 48 urs te oan ol sbomiralpainpeokingal 2thausandcecreasig win = "cay. Elevate Lipase values can also be cbsoved it ean pancroatsandebevulonal ha panreatedel Principle: Pancreat pate catalyses he yas of Tlin.n the presence of colpase to form monoayeres and fey feds. The ate of create tty massed at 3400, is prepertonal oth pase act The ails of fer pases ne serum anid byte cola sas in thereagent i one ago PL mpten Zoe Noma Reference Val Soum © uploT90UiL Ie recommenda that each boratry eben ts onn raralragerepeseningtspstenpapution. Contents sm) 25m 75m Lipase Reagent 10m 25m) TS :Calorstr (orm) Na Na. TN Storapestbilty Conan ae sil at 28°C the exp mantonadon the labls Donat teaze Reagent Preparation taLpase Reagents ready use Calibrator: Reconstiethe casraar wih‘ i of DY, ‘lon 10 sand To 10 mas wih ooeasonal ming, The recaatviad canola rales dye hen sored 125 and fort aa asks when tor at-20°. Do rolrepeaiay naw andretonze. Samplemteria Serum Fraoton Hemet, Upaseisrepaidtc ta sablinsorum or Sdeysat2- Procedure Ppt ino cas dy et ibs ltoled as Cabra (0) rates) ‘aalon € T Sequence iy oy pase Reagontty 10 19 Catbator(C) oe Samp oe bland nada al Car sanded eal isrbance Afar ha Caltyatr (0) and Tet (7). Road ther sosorvanc Av of te Calbraor and Test fer ‘exact § mins. Clete te change in absorbance AA for ba Be Catratarand Tet Fercaloeer AAC AcAC Forest oat ATAT Caleuttons ast Upaseinu - X Cone ofcalertr ae Unearty “The procedure is nes upo 700 UR. vais exceed is let ete sar wih noma sine (Nt 0) ad repeat he assay. Cle ha val upg te proper aluontacor Notes Reagents such Cholesterol Tlyerdes/HOL/LDL contain High Concentrations of detergents and hydrelysng enzymes, eross contamination fom such ‘agents sould beavelded Al glassware ips andcuvets being used forthe test shouldbe throughly leaned Samples having vary hgh acy show a vary owl ssoreance 9 mos he substrates consumed poo the tart of maaticoment is suspected Men cle the samples repeatha assy. Dort use Uses reagent the Incl sbsrbance is talon 70003340 magia In raze cases sone sara may give an incaase in ‘Ssorbanco, he Lipase acy of hase sampos usualy fatswihn Heros ange Conponents fom human arginine carlo have bon tele and fad be negave fre presence of HBSA, He, anc antbooy oH (12), However and catousy aspsantalyifctosReferences Zagantor J, la. Clin Chon 1978, 25.1087 “ate NW et al Cin Cen 1989; 39746785, Lot... tal. lin. Cer. 186.82 1820, “Tate Textook of Cia Chemisty. oa: WB Saunders Campany; 1298 686-708, ‘System Parameters Reaction Fd Tmo wih St Wavelength = 380nm Zoro Seting = Oisted Water Ineub. Temp. S7C Ineub: Tne DelayTime 240 soc, React Tine: 500308, Novofread. Interval ~ Sample Vol 004-1 ReagentVol, = 1.001 Standard Seo Collar value Fretor = React. Slope: Deceesng Linearity mou Unis 2 UR Gana, Dr Arana Oo Rega Bap, Banton Carpex PO. GOA. 408202, NDIA urns