Identification of ILI / SARI / ARI cases As per WHO case definition (Referred and Systematic collected samples)

Severe acute respiratory infections (SARI): An acute repiratory infection with a history of fever or measured fever of ≥ 38° C and cough with onset within the last 7
days and requires overnight hospitalization.
Influenza like Illness (ILI): Acute onset within the last 10 days following respiratory symptoms, measured fever of ≥ 38° C and cough.
Acute respiratory illness (ARI): Sudden onset of respiratory infection symptoms ( cough, sore throat shortness of breath, coryza)

Sample Collection: Optimum viability of Influenza virus is at 2-8oC; NP swab / Throat swab / Nasal aspirate (place on ice / cold packs at 2-80C /
40C for transport to lab) in vial containing 1-3 ml VTM. Swab should be collected with Synthetic tip (Polyster / Plastic shift)

Real time RT-PCR (test as soon as collected/ Referred)

Testing for other Respiratory

viruses
+ve Influenza virus

Typing by Real time RT-PCR Duplex RT-PCR

Respiratory Syncytial
+ve Influenza A virus +ve Influenza B virus Viruses RSV A & B
Real time PCR for lineages
Sub typing by Real time RT-PCR Para Influenza Viruses 1 to 4
Human metapneumo Viruses
Yamagata Victoria
A H1N1 virus A H3N2 virus Rhino virus
Adeno Virus
Allelic disremination rt RT-PCR for A(H1N1)pdm09

Influenza positive Isolation in MDCK Cell

Lines

Genetic Characterization Antigenic characterization

Sequencing of HA / NA
Phenotypic Assay
Antiviral Susceptibility