RAW DATA

DETERMINATION OF MINERAL ELEMENTS IN FOOD

PRODUCTS BY AAS (ASHING METHOD)

1. Absorbance Data for Samples:

Mineral measured: Na
Absorbance reading at λ: 589.0nm
ABSORBANCE
SAMPLE
1 2 3 Average ± S.D.
MUSHROOM 0.613 0.612 0.611
BABY CORN 0.757 0.758 0.756

Mineral measured: Ca
Absorbance reading at λ: 422.7nm
ABSORBANCE
SAMPLE
1 2 3 Average ± S.D.
MUSHROOM 0.035 0.035 0.035
BABY CORN 0.036 0.036 0.036
2. Absorbance Data for Standard Solution
(Do graph on “Average Absorbance against standard concentration”)

Mineral measured: Na
Absorbance reading at λ: 589.0nm
STANDARD ABSORBANCE
CONCENTRATIO Average ±
N (mg/l) 1 2 3 S.D
1 0.267 0.266 0.269
2 0.523 0.526 0.529
3 0.714 0.712 0.710
4 0.859 0.853 0.855
5 0.971 0.970 0.968
Mineral measured: Ca
Absorbance reading at λ: 422.7nm
STANDARD ABSORBANCE
CONCENTRATIO Average ±
N (MG/L) 1 2 3
S.D
1 0.082 0.082 0.082
2 0.134 0.134 0.133
3 0.190 0.190 0.190
4 0.242 0.241 0.242
5 0.295 0.295 0.296

CALCULATION:

A. Determination of sodium in canned food products

Take equation from curve,

y=0.2133 x

- Average absorbance of sodium in mushroom,

( y )=0.612
Thus,
0.612
x=
0.2133
mg
¿ 2.87
L

SODIUM CONTENT IN MUSHROOM,

mg 1L 1 1000 g
¿ 2.87 × × 50 mL × × ×250
L 1000 mL 10 g 1 kg
mg
¿ 3587.5
kg
 - Average absorbance of sodium in baby corn,

( y )=0.757
Thus,
0.757
x=
0.2133
mg
¿ 3.55
L

SODIUM CONTENT IN BABY CORN,

mg 1L 1 1000 g mg
¿ 3.55 × ×50 mL× × × 100=1775
L 1000 mL 10 g 1 kg kg

B. Determination of calcium in canned food products

Calcium content in mushroom,

Calcium content in baby corn,