Syllabus

Bioprocess Engineering (CHEG 304)

Chemical Engineering
3rd Year Ist Semester
Instructor: Dr. Bishnu Prasad Pandey, Assistant Professor
Chapter 1. Kinetics of Substrate Utilization and Product Formation [8 hrs]
1.1. Ideal Reactor for Kinetic Measurements
1.1.1. The Ideal Batch Reactor
1.1.2. The Ideal Continuous-Flow Stirred Tank Reactor (CSTR)
1.2. Kinetics of Balanced Growth
1.2.1. Monod Growth Kinetics
1.2.2. Kinetics Implications of Endogenous and Maintenance Metabolism
1.2.3. Other Environmental Factor on Growth Kinetics
1.3. Transient Growth Kinetics
1.3.1. Growth Cycle Phases for Batch Cultivation
1.3.2. Unstructured Batch Growth Models
1.3.3. Growth of Filamentous Organism

Chapter 2 Oxygen Transfer [4 hours]

2.1. Gas-Liquid Mass Transfer in Cellular System
2.1.1. Basic Mass-Transfer Concepts
2.1.2. Rates of Metabolic Oxygen Utilization
2.2. Determination of Oxygen Transfer Rates
2.2.1. Measurement of kla’ Using Gas-Liquid Reactions

Chapter 3 Design and Analysis of Biological Reactors [6 hrs]

3.1. Ideal Reactors
3.1.1. Fed-Batch Reactors
3.1.2. Enzyme-Catalyzed Reactor in CSTRs
 3.1.3. CSTR Reactors with Recycle and Wall Growth
3.1.4. The Ideal Plug-Flow Tubular Reactor
3.2. Reactor Dynamics
3.2.1. Dynamic Models
3.2.2. Stability

Chapter 4. Instrumentation and Control [8 hour]

4.1. Physical and Chemical Sensors for the Medium and Gases
4.1.1. Sensors of the Physical Environment
4.1.2. Medium Chemical Sensors
4.1.3. Gas Analysis
4.2. On-Line Sensor for Cell Properties
4.3. Off-Line Analytical Methods
4.3.1. Measurement of Medium Properties
4.3.2. Analysis of Cell Population Composition

Chapter 5. Product Recovery and Operation [10 hours]

5.1. Recovery of Particulates: Cells and Solid Particles
5.1.1. Filtration
5.1.2. Centrifugation
5.1.3. Sedimentation
5.1.4. Emerging Technologies of Cell Recovery
5.2. Product Isolation
5.2.1. Extraction: Solvent Extraction, Extraction using Aqueous Two-Phase System
5.2.2. Sorption
5.3. Precipitation
5.3.1. Procedure for Isolation of Enzymes from Isolated Cells
5.3.2. Kinetics of Precipitate Formation
5.4. Chromatography and Fixed-Bed Adsorption: Batch Processing with Selective Adsorption
5.5. Membrane Separation
5.5.1. Reverse Osmosis
5.5.2. Ultrafiltration

Chapter 6: Bioprocess Economics [10 hours]

6.1. Process Economics, Bioproduct regulation, General Fermentation process Economics, Example
6.2. Fine Chemicals
6.2.1. Enzyme
6.2.2. Protein and Recombinant DNA
6.2.3. Antiobiotcs
6.2.4. Vitamins, Alkaloids, Nucleosides
6.2.5. Monoclonal Antibodies (Mab)
6.3. Bulk Oxygenates
6.3.1. Brewing and Wine Making
6.3.2. Fuel Alcohol Production
6.3.3 Organic and Amino Acid Manufacture
6.4. Single- Cell Protein
6.5. Anaerobic Methane Production

Text Book:
1. Biochemical Engineering Fundamental , James E. Bailey and David F. OLLIS, Second Edition,
McGRAW-HILL International Editions
Reference book:
1. Introduction to Biochemical Engineering. DG RAO, The McGraw –Hill Companies,
2. Bioprocess Engineering Basic Concepts. Michael L. Shuler, Fikret Kargi, Second Edition, PHI
Learing Private Limited, New Delhi