Rotary Microtome CUT 4060: Operating Manual

microTec
Rotary Microtome CUT 4060
Operating Manual
Made in Germany
microTec Laborgeräte GmbH

Rudolf-Diesel-Str. 47
69190 Walldorf / Germany
phone. : + 49 (0) 6227 / 8222-0
fax: + 49 (0) 6227 / 8222-30
mail@micro-tec.de
www.micro-tec.de
Revision (February 2017)
microTec GmbH - Company for Manufacture and Export of Laboratory Equipment

1
microTec
Table of Contents
1. General Notes 4
1.1 Significance of the Operating Instructions 4
1.2 Usage According to its Specification 5
1.3 Improper Use 6
1.4 Warranty and Liability 6
1.5 Warranty 6
1.6 Brief Discription of Use, Function and Sequence 7
1.7 Edition, Date of Issue of the Operating Manual 8
1.8 Copyright 8
1.9 Address of Manufacturer 8
2. Basic Safety Instructions 9

2.1 Heed the Notes and Instructions Given in this Operating Manual 9
2.2 Obligation on the Part of the Operator 9
2.3 Obligations on the Part of the Operating Staff 9
2.4 Hazards and Dangers in Handling and Using the Instrument 10
2.5 Interpretation of Hazard Symbols 11
2.6 Informal Instructions Issued by the Manufacturer 12
2.7 Particular Zones of Danger 12
2.8 Servicing and Fault Clearance 13
2.9 Structural Modifications and Replacements to the Instrument 13
2.10 Cleaning and Associated Care 14
3. Transportation and Storage 15

3.1 Dimensions and Weights 15
3.2 Instructions for Transportation 15
3.3 Packaging 15
3.4 Inspection upon Acceptance by the Recipient 17
3.5 Reporting and Documenting Transportation Damage 17
3.6 Ambient Conditions 17
4. Data and Information Concerning the Instrument 18

4.1 Designation 18
4.2 Scope of Supply 18
4.3 Certification of Conformity, Manufacturer’s Declaration 19
4.4 Ambient Temperatures during Operation 20
4.5 Performance 20
5. Installation, Assembly, Commissioning 21

5.1 Installation 21
5.2 Transportation Locking Pieces and Securements 21
5.3 Controls, Indicators and Displays 22
6. Operation 24
6.1 Preparation, Orientation and Alignment of Specimen 24
6.2 Commencing the Cutting Operation 24
6.3 Instructions for Producing Sliced Microtomic Sections of the Specimen 24
6.4 Cutting Action 25
6.5 Handwheel (Fig. 1) 25
6.6 Handwheel 25
6.7 Coarse Advanced Drive 25
6.8 Friction Clutch on the Coarse Advanced Drive Mechanism 26

2
microTec
6.9 The Handwheel of the Coarse Advanced Drive Mechanism (11) 26
6.10 Collision Protection Device 26
7. Setting Up and Changing the Setup 27

7.1 Specimen Fixture 27
7.2 Standard Specimen Clamping Fixtures 27
7.3 Orientatable Adapter 28
7.4 Fixed Adapter 28
7.5 Cylindrical Specimen Insert and Prism 29
7.6 Film (Thin Material) Clamping Fixture 30
7.7 Universal Cassette Clamping Fixture 31
7.8 Specimen Orientation Device 32
7.9 Exchanging the Specimen Clamping Systems 33
8. Setting up the Knife 34

8.1 Knife Holder 34
8.2 Knife Holder Base 34
8.3 The “R“ Type Knife Holder 35
8.4 Holder for Throw-away Blades 36
8.5 Clearance Angle Adjustment 37
9. Additional Functions 38
9.1 Additional Extras on Model CUT 4060 38
10. Emergency Service, Customer Service 38

3
microTec
1. General Notes
1.1 Significance of the Operating Instructions
Dear Customer,
Please read through the operating instructions carefully before using the equipment and
familiarize yourself with its functions and controls. This is imperative to ensure proper
use of the equipment.
Please check the production serial number against the number given on the microTec
identification plate of your unit. It will facilitate faster service and response to queries by
quoting the production serial number.
microTec Production Serial-Number: 60-

phone. : + 49 (0) 6227 / 8222-0
fax: + 49 (0) 6227 / 8222-30
mail@micro-tec.de
www.micro-tec.de

4
microTec
1.2 Usage According to its Specification

The CUT 4055 and CUT 4060 rotary microtomes are manually operated instruments for
use in all work involving paraffin wax and solid cut microtomology within the disciplines of
biology and medicine, and within industry at large.
The specimen can be moved back and forth by means of the coarse wheel mechanism so
as to attain rapid advance of the specimen in relation to the knife during cutting.
The micro-drive mechanism enables feed settings to be made within the range of 0,5 to
60 microns.
The user friendly throw-away blades and universal knife holder with its integrated knife
guard enables trouble-free clamping and setting of the knife blade. The integrated safety
device to prevent any collisions occurring, which is incorporated in the micrometer
mechanism, disables any adjustment to the feed setting being made upon reaching its
maximum setting.
The CUT 4060 rotary microtome is equipped with an electronic cutting counter, which
indicates the total number of cuts made.
To prevent damage occurring to the knife or the specimen, the rotary CUT 4060
microtome imparts an action of slight retraction of the specimen by approx. 80 microns
during the motion of advancement. Likewise, a three-position trimming assembly is also
incorporated (10, 20 and 30 micron trimming).

5
microTec
1.3 Improper Use

The rotary microtome is designed and manufactured to the latest state-of-the-art
technology and to recognized principles of technical and engineering safety.
Nevertheless, dangers can arise during its use to endanger life and limb of the operator or
third persons or damage to the instrument or to other property. The instrument is only to
be used
 for use according to its specification of intended use

 in a proper technical state of repair, free of any defects
1.4 Warranty and Liability

The company – microTec GmbH – certifies that this instrument has been carefully tested
and inspected. Verification to ensure adherence to the technical specification data
published has been carried out prior to shipping out the instrument from the factory.
The instrument is assigned to Class 4 of the MedGV (legislation governing medical

supplies). Observance of existing legislation and regulation is assured.
1.5 Warranty
Warranty is provided for this microTec product in the event of any deficiencies or defects
in material or workmanship fpr a period of 12 months. Defective parts will be repaired or
replaced free of charge during this period of time. Any additional claims made under the
warranty of whatsoever nature are excluded. Neither does the warranty provide cover for
any claims arising from services and supplies provided by third parties.
In principle, our “General Conditions of Sale and Supply“ shall apply. These conditions
are made available to the prospective operator of the instrument, by, at the latest, the
signing of the contract. Warranty and liability claims for personal injury and material
damage are excluded if attributable to one or more of the following causes:
 Improper use of the instrument!

 Incorrect installation and assembly, commissioning, operation and servicing of the
instrument!
 Operating the instrument with the safety devices defective or safety mechanisms
and guards inappropriately fitted!
 Disregarding instructions given in the operating manual concerning transportation,
storage, installation, commissioning, servicing and equipping of the instrument!
 Unauthorized tampering and structural modification to pneumatic, electrical and
mechanical components!
 Ineffective or incorrect monitoring of machine parts which are subject to wear!
 Improperly effected repairs!
 Catastrophes caused by external influences and force majeure!

6
microTec
1.6 Brief Description of Use, Function, and Sequence
Use
The CUT 4055 and CUT 4060 rotary microtomes are manually operated instruments for
use in all work involving paraffin wax and solid cut microtomology within the disciplines of
biology and medicine, and within industry at large.
Function and Sequence

Manually rotating the handwheel on the rotary microtome translates into vertical
movement of the fixture holding the specimen. To produce the cut, resharpenable knives
or throw-away blades are used, which are adapted and attached to the various knife
holders.
A microtomic cut is made during the downward motion of the specimen (cutting action).
By continuing to rotate the handwheel further in a clockwise direction the specimen starts
to rise again in its return motion.
The cutting feed can also be set by means of the three-position trimming assembly (only
available on the CUT 4060 model).
The selected feed for the thickness of cut is instigated at top dead center.
Rapid advance of the specimen towards the knife is achieved by means of the coarse
feed mechanism.
A universal clamping fixture for holding a variety of specimens is provided.

As a result, simple alignment to the plane of cut is possible using the facility for orientating
the specimen.
The various knife holders enable trouble-free use of a variety of knife types and thereby
provide facility for making adjustment to suit the application in question.

7
microTec
1.7 Edition, Date of Issue of the Operating Manual

Edition: Version 004.96
Date of issue: 13. February 1996
1.8 Copyright
Copyright : Firma microTec GmbH
Copyright to this operating manual remains with microTec GmbH. This operating manual
is only intended for use by the operator and his staff.
It contains specifications and instructions, which may not be

- copied,
- distributed or
- otherwise divulged to others in their entirety or in part.
Infringements can lead to legal proceedings being brought against the perpetrators.
1.9 Address of the Manufacturer

Rudolf-Diesel-Straße 47
phone: + 49 (0) 6227 / 82 22-0
fax: + 49 (0) 6227 / 82 22-30
mail@micro-tec.de
www.micro-tec.de

8
microTec
2. Basic Safety Instructions

2.1 Heed the Notes and Instructions Given in this Operating Manual!
A prime prerequisite for safe handling and malfunction-free operation of this insturment is
the awareness and recognition of basic safety instructions and regulations.
This operating manual contains the most important instructions for operating the
instrument in a safe manner.
This operating manual, especially the instructions on safety, are to be heeded by all
personnel who operate the instrument.
In addition, compliance with the regulations and specifications on accident prevention
applicable for the site of installation and use is to be maintained.
2.2 Obligation on the Part of the Operator

The operator is obliged to only allow personnel to operate the instrument, who
 are familiar with basic regulations on safety at work and accident prevention, and are
trained in the use and operation of the instrument.
 have read the chapter on safety and the warning notes and instructions given in this
operating manual.
 have been taught to have due regard to aspect of safety during the execution of their
work.
2.3 Obligations on the Part of the Operating Staff

All personnel who are delegated with the task of operating this instrument are obliged
before starting work
 to heed the basic regulations on safety at work and accident prevention.
 to read and to comprehend the chapter on safety and the warning notes and
instructions given in this operating manual.

9
microTec
2.4 Hazards and Dangers in Handling and Using the Instrument
Important!
The following precautionary measures must be

undertaken during any operation carried out using the
instrument. Non-compliance with these precautionary
measures will infringe recognized principles and rules
of technology and the intended and proper use of the
instrument. The manufacturer – microTec GmbH –
accepts no liability in the event of such infringement.
Risk of Injury
The finger guard must be used when setting the knife
and specimen, in order to reduce the risk of injury on
the knife/blade.
Always insert the specimen first.

Then insert the knife into the knife holder.
Before exchanging the clamping device, always

remove the knife or blade. Knives, which are not
being used, must always be stored in the knife box.
Never attempt to catch a falling knife. Take care when
cutting or removing a sliced section.
Risk of Infection
Appropriate measures regarding protection and
disinfection are to be taken when working with
infected material.
Danger due to Radio Activity

The current applicable regulations for the protection
against radiation must be observed at all times when
working with radio active materials or specimens.

10
microTec
2.5 Interpretation of Hazard Symbols
Interpretation Symbol
 Danger
 Warning
 Caution
 Note
 Important !
 Information

11
microTec
2.6 Informal Instructions Issued by the Manufacturer
Preparation of the Specimen
Due attention should be paid in the preparation of specimens with regard to making the
correct choice of the medium used for embedding and encapsulation of the specimen and
its curing and infiltration time.
Temperature of the Specimen

Microtomic sectioning is generally carried out at room temperature (except in the case of
frozen specimens). Increases in temperature will soften the paraffin wax. The heating up
of paraffin wax specimens due to direct exposure to sunlight or their proximity to any
other source of heat is to be avoided.
Choosing the Knife

Depending upon the ground facet in question, a clearance setting at an angle of 18 to 20
degrees is to be selected. In the case of re-sharpened knives, use the knife height
adjustment facility to set the height of the knife.
Levers and Clamping Screws

Securely tighten all levers and clamping screws on the knife holder and the specimen
orientation device. -> Overtightning could result in damage of knife holder!!
Cutting Speed
Rule: “The harder the material, the slower the cutting speed“.
Starting the Cut

Take care when approaching the knife or the specimen.
2.7 Particular Zones of Danger
Important!
Knives are sharp!
Always set the knife guard in its proper position after completing the operation – even if
it’s for a brief moment of time – and make sure it is properly seated.

12
microTec
2.8 Servicing and Fault Claerance
Servicing
To secure the quality of the microtomic section and operational reliability of the rotary
microtome, a routine service should be carried out once a year. Servicing should only be
carried out by a trained service engineer.
Service Contract
microTec offers a service contract which guarantees that your instrument will remain in a
perfect operational condition.
Fault Clearance
Malfunctions, which can adversely affect safety, are to be rectified immediately.
2.9 Structural Modifications and Replacements to the Instrument
 No modifications, conversions or enhancements may be made to the instrument

without the approval being given by the manufacturer.
 All conversions require written confirmation issued by microTec.
 Replace components which are not in proper working order immediately.
 Only use original replacement and consumable parts and items.
 It cannot be guaranteed that parts sourced from other suppliers are designed
and manufactured to meet safety and reliability conditions.

13
microTec
2.10. Cleaning and Associated Care
Cleaning
Depending upon the level of dirt and contamination and
upon the frequency of use, regular cleaning and care of
the rotary microtome is recommended. The instrument is
best cleaned using a mild domestic cleaning agent. Do
not use harsh solvents or cleaning compounds, because
paintwork and plastic components can be damaged by
their use.
Always remove the knife and stow in the knife box

prior to cleaning the instrument.
Important!
Never place a knife on a table or surface with the cutting

edge facing upwards. Thoroughly clean the bearing
surfaces and contact faces and the guide of the knife
holder.
Associated Care
When the instrument is not in use, turn back the cylinder

by means of the coarse drive mechanism and lock the
handwheel. Cover the instrument with the cover supplied.

14
microTec
3. Transportation and Storage

3.1 Dimensions and Weights
Dimensions
Width.…….……………………. 460 mm
Depth…….…………………….…460 mm
Height..…………………………...280 mm
Weight
Weight (without accessories)……..36 kg
3.2 Instructions for Transportation

Lock the handwheel.
Remove all knives, knife holders, specimen
fixtures and specimens from the rotary microtome.
3.3 Packaging
The rotary microtome has been supplied in a
special packaging designed for transportation. Do
not commission (start up) the instrument until al
least 24 hours have elapsed following
transportation.
Please ensure the specified procedures are strictly
observed!
Transportation crate
unopened.

15
microTec
3.4 Inspection upon Acceptance by the Recipient

Firstly, check that all items ordered are supplied in the transportation crate. Inspect the
transportation crate and the rotary microtome for any damage incurred during transit.
3.5 Reporting and Documenting Transportation Damage

In the event of damage caused in transit, the manufacturer and the forwarding agent are
to be advised immediately in writing.
3.6 Ambient Conditions

Storage temperature range -20° C to + 50° C.

16
microTec
4. Data and Information Concerning the

Instrument
4.1 Designation
Rotary Microtome, model CUT 4060
4.2 Scope of Supply, i.e.

According to order confirmation.

17
microTec
4.3 Certification of Conformity, Manufacturer’s Declaration
Declaration of Conformity
We declare under our sole responsibility that the product
Rotary Microtome CUT 4060 incl. Accessories
Model Designation: CUT 4060 – Serial-No. 60-1326
meets all the requirements of the following directives, that are applicable.
98/79/EEC In Vitro Diagnostic Directive
Classification acc. to IVDD 98/79/EEC Other devices
Applied harmonised standards, national DIN EN 292, safety of the machines,

Standards or other normative documents equipment and systems
Address of the Manufacturer

Walldorf, 20th January, 2017 Walter Ganter

Place, Date Signature Information of the Signatory

18
microTec
4.4 Ambient Temperatures During Operation

- 10° C to + 45° C
4.5 Performance
Cut thickness range: …………………....….…...………………..............................0,5 – 60 microns
Progression: ..……………………………………………………………0,5 microns of 0,5 - 2 microns
1 micron of 2 - 10 microns
2 microns of 10 - 20 microns
5 microns of 20 - 60 microns
Horizontal specimen movement:………..…..………………………..............................max. 40 mm

Vertical specimen movement:………..……………………………….............................max. 70 mm
Specimen size: Standard……..…………….... ................55 x 50 mm

Specimen orientation: X- and Y-axes…..........….………...............universal
Z- axis……….…...…………......................up to 360°
Cutter drive:……………………………………...…...........................………..manual via handwheel

Coarse advance:…………………………………….. …................................manual via handwheel

19
microTec
5. Installation, Assembly, Commissioning

5.1 Installation
Protect the instrument from:

 sources of heat such as fan heaters or hot air outlets ;
 extremely low temperatures;
 direct sunlight;
 excessive dust;
 moisture or rain;
 mechanical vibration or impacts;
 equipment which contain magnets or generate magnetic fields.
5.2 Transportation Locking Pieces and Securements

Remove all locking pieces and adhesive tape used for securement during transportation.
Place the instrument on a rigid, vibration-free table or bench.

Align the instrument on the table. Lift up the instrument a little at the front.

20
microTec
5.3 Controls, Indicators, and Displays
Item Description
1 Handwheel for cutting operation
2 Handwheel handle for handwheel 1
3 Knob for releasing the handwheel locking device 4
4 Handwheel locking device
5 Rotary knob for setting the thickness of slice
6 Inspection window for setting and inspecting slice thickness
7 Locking lever for knife holder
8 Lever for trimming adjustment*
9 LC display (cutting counter)*
10 Reset for LC display*
11 Handwheel for coarse drive
12 Cylinder
13 Clamping mechanism for knife holder
14 Guide bars for knife holder

21
microTec
5.3 Controls, Indicators and Displays
LCD-Display
Inspection window and

rotary knob
Fig. 1
* Only on CUT 4060 model
Fig. 2

22
microTec
6. Operation
6.1 Preparation, Orientation, and
Alignment of the Specimen
Prior to starting to cut the specimen, the specimen
must be suitably prepared and embedded and
encapsulated in an appropriate medium. Various
molds and plastic cassettes are used for
encapsulation purposes.
By using the microTec adapter system, the

specimen can be aligned to the plane of cut.
6.2 Commencing the Cutting

Operation
Macro positioning of the specimen in relation to
the knife cutter is accomplished by moving the
base of the knife holder, or by operating the
handwheel of the coarse feed drive (Fig. 2.11).
With continued operation, cutting of the specimen

commences up to the required depth of slice. The
residues of the cut specimen so produced must be
wiped away from the knife in an upwards direction
from underneath using a brush.
Important!
Do not touch the knife with your fingers!
6.3 Instructions for Producing Sliced

Microtomic Sections of the
Specimen
The following aspects are to be heeded in order to
produce effective sliced microtomic sections of the
specimen:
 always use a sharp knife,

 as wear occurs at the point of cut, slide the
knife to the left or to the right, in order to be
able to continue working with a sharp section
of the knife,
 always keep the front and rear face of the knife
clean. It is important to thoroughly remove any
traces of paraffin wax.

23
microTec
6.4 Cutting Action

The cutting action of the rotary microtome is
generated by rotating the handwheel (Fig. 1.1) in a
clockwise direction. The cut is accomplished on the
down stroke and retraction on the upwards directed
stroke by further rotation of the handwheel (Fig. 1.1)
in a clockwise direction.
6.5 Handwheel (Fig. 1)

The handwheel (1) is integrated in the casing of the
microtome (Fig. 1).
The position of the handle (2) corresponds to the
vertical position of the specimen.
The handwheel locking device is situated on the
handwheel (1).
Releasing the handwheel locking device

Press down the knob (refer to illustration)
Locking the handwheel (1)

Press the handwheel locking device (refer to
illustration).
Handwheel (1) engages in one of the 24 locking

positions.
Important!
For personal safety, the handwheel should

always be locked in the upper region of vertical
travel when exchanging specimens.
6.7 Coarse Advanced Drive

To rapidly alter the distance between the knife
and the specimen, the rotary microtome is
equipped with a coarse advancement drive (11).
The specimen fixture can be moved forwards or
backwards horizontally by the handwheel for the
coarse advancement drive (11).

24
microTec
6.8 Friction Clutch on the Coarse Advanced Drive Mechanism

On reaching the front and rear positions of the specimen feed, a friction clutch built into the
coarse advanced drive prevents the blocking of this coarse advanced drive. As soon as an
audible engagement is triggered by the coarse drive, the feeding operation of the specimen
stops. Upon rotation of the handwheel of the coarse advanced drive (11) in the opposite
direction, the feeding motion is restored.
If the feeding motion is not restored by this procedure, the handwheel of the coarse
advanced drive mechanism (11) must be pressed inwards against the casing. Only a short
forward or backward movement is needed to ultimately activate the feeding motion.
Important!
Avoid touching the specimen or the specimen holder containing the knife when
operating the handwheel of the coarse advanced drive mechanism.
6.9 The Handwheel of the Coarse Advanced Drive Mechanism (11)

The handwheel of the coarse advanced drive mechanism (11) is integrated in the casing
(Fig. 2)
6.10 Collision Protection Device

On the specimen feed system reaching its forward
terminal position, a red mark is visible on the
cylinder (12). From this mark there is a further
distance of adjustment of max. 2500 microns
available. A second red mark then becomes
visible, and the cylinder must be rotated back by
means of the handwheel of the coarse advanced
drive (11), before further cuts can be made.
Important!
As soon as the remaining distance of adjustment of 2500 microns is exceeded, the
preselected thickness of cut is disabled by the in-built collision protection device.
Any automatic adjustment by rotating the handwheel is now inactivated.

25
microTec
7. Setting Up and Changing the Setup

7.1 Specimen Fixture
To hold the specimens during cutting, various adapters and clamping fixture systems are
available, based on the microTec principle of modular construction. In conjunction with the
specimen orientation device, it is possible to orientate and align the specimen to the plane
of cut is simple manner. An adapter piece is inserted at the orientation point where
operations do not require such orientation.
7.1 Standard Specimen Clamping Fixtures
The standard specimen clamping fixture (Fig. 3) is used for paraffin wax or plastic molded
blocks of square or rectangular form. When using, the specimen is placed against the top
fixed jaw (Fig. 3 – item 1) and clamped by the clamping screw (Fig. 3 – item 2).
Important!
On the grounds of stability, the specimen
should not be allowed to protrude too far
beyond the clamping jaws.
Fig. 3

26
microTec
7.3 Orientatable Adapter

The orientatable adapter (Fig. 3 – item 3) can optionally be attached by screws to the
various specimen clamping systems and be used only in combination with the specimen
orientation device.
Specimen clamping systems of orientatable design are supplied ex works equipped with
the orientatable adapter.
Example (Fig. 3)
The orientatable adapter (Fig. 3 – item 3) is shown in this illustration attached to the
standard specimen clamping fixture by 4 screws (Fig. 3 – item 4) and can be removed if
required.
Dismantling the standard specimen clamping fixture: Loosen 4 screws

(Fig. 3 – item 4)
7.4 Fixed Adapter

This adapter (Fig. 4 – item 1) is used as a fixed attachment directly on to the cylinder (Fig.
4 – item 2), and can be optionally fitted to various specimen clamping systems (Fig. 4).
Fig. 4

27
microTec
7.5 Cylindrical Specimen Insert and Prism

To clamp cylindrical specimens (Fig. 5), the cylindrical specimen insert (Fig. 5 – item 1), or
the prism (Fig. 5 – item 2), can be inserted into the standard specimen clamping fixture
(Fig. 5 – item 3), having the diameters
- Ø 6 mm
- Ø 15 mm
- Ø 25 mm
- Ø special diameters on request
The location pin (Fig. 5 – item 4) engages the movable clamping jaw. Springs (Fig. 5 –
item 5) on the cylindrical specimen insert (Fig. 5 – item 1) enable easy removal of the
cylindrical specimens from the inserts.
Fig. 5

28
microTec
7.6 Film (Thin Material) Clamping Fixture

The film clamping fixture (Fig. 6) is a clamping system for film and foil materials or flat and
thin specimens. To insert the specimen, the three threaded pins (Fig. 6 – item 4) are
loosened, and the movable jaw (Fig. 6 – item 2) against the two compression springs, the
setting of which is made by the two threaded pins (Fig. 6 – item 3), moved laterally. The
specimen is held by the force of the springs, but can be repositioned. By screwing in the
threaded pins (Fig. 6 – item 4), the specimen is securely clamped between the jaws of the
film clamping fixture.
Fig. 6

29
microTec
7.7 Universal Cassette Clamping Fixture

The universal cassette clamping fixture (Fig. 7) is a quick change specimen system, and
is used for the clamping of cassettes.
Inserting and removing the cassette

Pull clamping lever (Fig. 7 – item 1) in the direction
of the arrow (Fig. 7)
Important!
Optimal clamping is only achieved
if the bearing faces are clean of any
paraffin wax residues.
Fig. 7

30
microTec
7.8 Specimen Orientation Device

Specimen clamping systems are attached to the orientation device (Fig. 8) by means of
the orientatable adapter (Fig. 8 – item 3). This way, orientation of the specimen in relation
to the plane of cut is accomplished.
Rotate the eccentric cam lever (Fig. 8 – item 1) towards the front.
Specimen clamping fixture is released. Range of specimen clamping: 360º (Z axis).
Turn orientation screws (Fig. 8 – item 2)

It is possible to orientate the specimen 8º in each direction (X axis, Y axis).
The spring loaded pin (Fig. 8 – item 3) acts upon the orientation screws (Fig. 8 – item 2).
After orientating the specimen, press the eccentric cam lever (Fig. 8 – item 1) upwards
into the clamping position.
Specimen is clamped.
Cutting of the specimen can now commence.
Important!
The spring loaded pin (Fig. 8 – item 3) must
remain fully screwed in, in order to keep the
orientation screws loaded under spring
pressure
Fig. 8

31
microTec
7.9 Exchanging the Specimen Clamping Systems

Exchanging the specimen clamping system
Release (press downwards) the eccentric cam lever (Fig. 8 – item 1).
Withdraw from the left out of the specimen orientation device (Fig. 8).
Screw out the orientation screws (Fig. 8 – item 2) and spring loaded pin (Fig. 8 – item 3) a
little way.
Withdraw the specimen clamping fixture from the front from the specimen orientation
device.
Important!
After exchanging the clamping system, make sure that
the spring loaded pin (Fig. 8 – item 3) is screwed in
again, otherwise there will be insufficient spring
pressure imparted.
Note
In order to increase leverage at the handle of the eccentric cam
lever (Fig. 8 – item 1), the handle sleeve (Fig. 8 – item 4) can be
pulled out. After releasing, the handle sleeve springs back into its
nominal position.

32
microTec
8. Setting up the Knife

8.1 Knife Holder
The knife holders are user friendly designed
and easy to clean due to its special surface
treatment. The risk of injury during the setting
up of the knife and the specimen is minimized
by a laterally movable finger guard.
8.2 Knife Holder
The knife holder base is designed for

universal use, and can be used for the “R“
type knife holder and also for the holder for
throw-away blades.
Mounting the base of the knife holder (Figs. 9 + 10).
Fig. 2
Release clamping lever (Fig. 2 – item 7) by rotating to the left.
Push the base of the knife holder on the guide bars (Fig. 2 – item 5).
Push the base of the knife holder centrally over the clamping mechanism (Fig. 2 – item 5).
Take note of the prism guide.
When in the required position, rotate clamping lever (Fig. 2 – item 4) to the right.
Allow the cutting edge of the knife to align flush with edge1. The height is adjusted by the
knurled screw (Fig. 9 – item 7). Clamping is accomplished by means of the clamping
screws (Fig. 9 – item 4).
“R“ type knife holder
angle marking
Fig. 9 Base of knife holder

33
microTec
8.3 The “R“ Type Knife Holder

Assembling the “R“ type knife holder on the base of the knife holder
Release clamping lever (Fig. 9 – item 1) in opposite
direction to that of the arrow.
Introduce the “R“ type knife holder into the clamping
mechanism (Fig. 9 – item 2).
Clamp at the required clearance angle position (Fig. 9 –
item 5).
Inserting the knife

Slightly unscrew clamping screws (Fig. 9 – item 4).
Push the knife in from the side.
or
Hinge up knife guard (Fig. 9 – item 3) in direction of arrow.
Insert knife from above.
Clamp knife by means of the clamping screws (Fig. 9 – item 4).
Push finger guard (Fig. 9 – item 6) to center each time when
Carrying out setting-up operation.
Clamping levers are fitted to both sides of the knife holders to facilitate easier operation.
Knife height adjustment device

Loosen clamping screws (Fig. 9 – item 4).
Adjustment to the height of the knife (1) is accomplished by screwing the knurled screws
(Fig. 9 – item 7) to the left or the right.
Tighten the clamping screws (Fig. 9 – item 4).
“R“ type knife holder
angle marking
Base of knife holder
Fig. 9

34
microTec
8.4 Holder for Throw-away Blades

Assembling the holder for throw-away blades on the base for the knife holder.
Release clamping lever (Fig. 9 – item 1) in opposite direction to that of the arrow.
Introduce the holder of the throw-away blades into the clamping mechanism (Fig. 9 – item 5).
Clamp at the required clearance angle position (Fig. 9 – item 5).
Inserting the throw-away blades

Release clamping lever (Fig. 10 – item 3).
Insert throw-away blades from the side in the gap between the bars and the clamping plate
(Fig. 10 – item 4).
Push throw-away blades to the center of the throw-away blade holder.
Tighten clamping lever (Fig. 10 – item 3).
Knife blades and associated clamping plates (Fig. 9b)
Part No. Blade Manufacturer Size Designation

00000221 ACCU-EDGE FEATHER 0,25 x 0,8 CUT 2020A Low Profil I
00000222 H-DMB-A0 TBS 0,32 x 14,1 CUT 2020B Low Profil I
ACCU-EDGE FEATHER 0,32 x 14,1 CUT 2020B High Profil I
Reichert # 818 C.M.S. 0,32 x 14,1 CUT 2020 High Profil I
00000223 H-DMB TBS 0,32 x 12,6 CUT 2020D High Profil III
00000224 # 244028 C.M.S. 0,89 x 15,6 CUT 2020C High Profil II
00000225 H-DMB-HD TBS 0,52 x 12,63 CUT 2020F High Profil V
00000226 # D544G Sturkey 0,44 x 14,0 CUT 2020E High Profil IV
# 2349 Shandon 0,44 x 14,0 CUT 2020E High Profil IV
Holder for throw-away blades
Space for inscription of

Clamping plate
Fig. 9b
Fig. 9a Base for Knife holder

35
microTec
Utilization of the blades

In order to utilize the entire cutting edge of the blades. These blades can be pushed to the right
or to the left.
Push blade with the clamping plate to the left or the right.
Tighten clamping lever (Fig. 10 – item 3).
Holder for throw-away blades
Space for inscription of

clamping plate
Fig. 9b
Base for Knife holder
Fig. 10
8.5 Clearance Angle Adjustment

The clearance angle between the knife cutting edge and the specimen can be adjusted as
required to conform to the respective cutting requirements. The clearance angle setting is
made by means of the clamping lever (Fig. 9 tem 1) at the base of the knife holder on all knife
holder systems.
Clearance angle setting

Pivot the knife holder and /or the holder of the throw-away blades into the clearance position.
The clearance angle can be read off from scale markings on both sides as an actual value.
Tighten the clamping lever (Fig. 9 – item 1).
Empirically, suitable microtomic sections are

produced at a clearance angle of 15º. This
clearance angle position is characterized by a
longer mark on the knife holder base.

36
microTec
9. Additional Functions
9.1 Additional Extras on Model CUT
4060
Specimen retraction on upward stroke
The specimen retraction device instigates
mechanical retraction of the cylinder during upward
motion.
Retraction by approx. 80 microns gives added care
to protecting and extending the life of the knife and
the specimen.
Cutting counter with 6-digit. LCD display

By pressing the RESET knob (10), the cutting
counter is set to zero.
After each complete cutting stroke the cutting
counter increases the total of cuts by one.
The battery of the cutting counter has a life of
approx. 5 years.
Only allow a service engineer to replace the battery.
Trimming system – adjustable to three setting

levels
Trimming level 1: 10 microns
10. Emergency Service, Customer

Service
phone: + 49 (0) 6227 / 8222-0
fax: + 49 (0) 6227 / 8222-30
mail@micro-tec.de
www.micro-tec.de

37
microTec
1st Step
Remove all hexagon headed screws
on the sides of the crate and lift off the
complete cover of the transportation
crate.
2nd Step
With both hands lift the instrument out
from the transportation crate.
Important!
The unit is heavy – do not lift or carry

the instrument by means of the
handwheel.

38
microTec
ATTENTION!!!
Please read the enclosed

instruction before opening the
wooden crate!!!
ATTENTION!!!
Please read the enclosed

instruction before opening the
wooden crate!!!

39

Rotary Microtome CUT 4060: Operating Manual

Uploaded by

Copyright:

Available Formats

You might also like

Rotary Microtome CUT 4060: Operating Manual

Uploaded by

Document Information

Original Title

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

Rotary Microtome CUT 4060: Operating Manual

Uploaded by

Copyright:

Available Formats

microTec

Rotary Microtome CUT 4060

microTec Laborgeräte GmbH

microTec GmbH - Company for Manufacture and Export of Laboratory Equipment

2. Basic Safety Instructions 9

3. Transportation and Storage 15

4. Data and Information Concerning the Instrument 18

5. Installation, Assembly, Commissioning 21

microTec GmbH - Company for Manufacture and Export of Laboratory Equipment

7. Setting Up and Changing the Setup 27

8. Setting up the Knife 34

10. Emergency Service, Customer Service 38

microTec GmbH - Company for Manufacture and Export of Laboratory Equipment

microTec Production Serial-Number: 60-

microTec Laborgeräte GmbH

microTec GmbH - Company for Manufacture and Export of Laboratory Equipment

1.2 Usage According to its Specification

microTec GmbH - Company for Manufacture and Export of Laboratory Equipment

1.3 Improper Use

 for use according to its specification of intended use

1.4 Warranty and Liability

The instrument is assigned to Class 4 of the MedGV (legislation governing medical

 Improper use of the instrument!

microTec GmbH - Company for Manufacture and Export of Laboratory Equipment

1.6 Brief Description of Use, Function, and Sequence

Function and Sequence

A universal clamping fixture for holding a variety of specimens is provided.

microTec GmbH - Company for Manufacture and Export of Laboratory Equipment

1.7 Edition, Date of Issue of the Operating Manual

It contains specifications and instructions, which may not be

1.9 Address of the Manufacturer

microTec GmbH - Company for Manufacture and Export of Laboratory Equipment

2. Basic Safety Instructions

2.2 Obligation on the Part of the Operator

2.3 Obligations on the Part of the Operating Staff

 to heed the basic regulations on safety at work and accident prevention.

microTec GmbH - Company for Manufacture and Export of Laboratory Equipment

The following precautionary measures must be

Always insert the specimen first.

Before exchanging the clamping device, always

Danger due to Radio Activity

microTec GmbH - Company for Manufacture and Export of Laboratory Equipment

microTec GmbH - Company for Manufacture and Export of Laboratory Equipment

Temperature of the Specimen

Choosing the Knife

Levers and Clamping Screws

Starting the Cut

2.7 Particular Zones of Danger

microTec GmbH - Company for Manufacture and Export of Laboratory Equipment

2.9 Structural Modifications and Replacements to the Instrument

 No modifications, conversions or enhancements may be made to the instrument

 All conversions require written confirmation issued by microTec.

 Replace components which are not in proper working order immediately.

 Only use original replacement and consumable parts and items.

microTec GmbH - Company for Manufacture and Export of Laboratory Equipment

Always remove the knife and stow in the knife box

Never place a knife on a table or surface with the cutting

When the instrument is not in use, turn back the cylinder