Scribd Logo
Upload

Welcome to Scribd!

  • Eba Medium: © 2007 DSMZ GMBH - All Rights Reserved

    Uploaded by

    Siddiqui Imran
    13 views1 page
    This document provides the ingredients and preparation instructions for EBA MEDIUM, which is used for cultivating bacteria. It contains 0.20 g KH2PO4, 1.00 g NaCl, 0.50 g KCl, 0.50 g MgSO4 x 7 H2O, 0.15 g CaCl2 x 2 H2O, 0.25 g NH4Cl, 1.00 ml of trace element solution SL-10, 1.00 mg Resazurin, 1.00 ml Na2SeO3 x 5 H2O, 2.00 g Glycine, and 950.00 ml distilled water, with the pH adjusted to 7.2 to 7.4

    Original Description:

    Hjj

    Original Title

    DSMZ_Medium454

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Report this Document
    This document provides the ingredients and preparation instructions for EBA MEDIUM, which is used for cultivating bacteria. It contains 0.20 g KH2PO4, 1.00 g NaCl, 0.50 g KCl, 0.50 g MgSO4 x 7 H2O, 0.15 g CaCl2 x 2 H2O, 0.25 g NH4Cl, 1.00 ml of trace element solution SL-10, 1.00 mg Resazurin, 1.00 ml Na2SeO3 x 5 H2O, 2.00 g Glycine, and 950.00 ml distilled water, with the pH adjusted to 7.2 to 7.4

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pdf or txt
    13 views1 page

    Eba Medium: © 2007 DSMZ GMBH - All Rights Reserved

    Uploaded by

    Siddiqui Imran
    This document provides the ingredients and preparation instructions for EBA MEDIUM, which is used for cultivating bacteria. It contains 0.20 g KH2PO4, 1.00 g NaCl, 0.50 g KCl, 0.50 g MgSO4 x 7 H2O, 0.15 g CaCl2 x 2 H2O, 0.25 g NH4Cl, 1.00 ml of trace element solution SL-10, 1.00 mg Resazurin, 1.00 ml Na2SeO3 x 5 H2O, 2.00 g Glycine, and 950.00 ml distilled water, with the pH adjusted to 7.2 to 7.4

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pdf or txt
    of 1

    454.

    EBA MEDIUM

    KH2PO4 0.20 g
    NaCl 1.00 g
    KCl 0.50 g
    MgSO4 x 7 H2O 0.50 g
    CaCl2 x 2 H2O 0.15 g
    NH4Cl 0.25 g
    Trace element sol. SL-10 (see medium 320) 1.00 ml
    Resazurin 1.00 mg
    Na2SeO3 x 5 H2O 1.00 ml
    (0.26 g/ 1000 ml 10 mM NaOH)
    Glycine 2.00 g
    Distilled water 950.00 ml
    pH 7.2 to 7.4

    Before inoculation add per 100 ml of medium:


    Biotin (10 mg/l) 0.20 ml
    (autoclave for 10 minutes under N2)
    NaHCO3 (84 g/l) 3.00 ml
    (autoclaved under CO2)
    Na2S (60 g/l) 1.00 ml
    (autoclaved under N2)

    © 2007 DSMZ GmbH - All rights reserved

    You might also like