Doce, Joshua Mar Bmls-1A

1. What is the order of draw for ETS & Skin puncture?

Proper collection is important to the specimen integrity. The collection tubes

contain different types of additives, which are specific for the individual test.

For the order of draw in ETS is:

file:///C:/PCCD/Downloads/Order
%20of%20Draw%20(1).pdf

For the order of draw in Skin puncture is:

As CLSI established the order of draw for capillary specimens,

First — EDTA tubes; Second — other additive tubes; Third — non-additive

tubes.

https://www.phlebotomy.com/pt-stat/stat0908.html
 2. What is the importance why we should follow the correct order of draw?

We all know that order of draw in which the tubes are drawn is one of the most
important considerations when collecting blood specimens. So it must be collected in a
specific order to prevent the invalid test results caused by contamination of the
specimen by microorganisms or carryover of additives or anticoagulant between the
tubes. All additive tubes should be filled to their stated volumes. So an incorrect order of
draw could be mitigate the patient safety.

https://www.testmenu.com/musclabservices/TestDirectory/SiteFile?fileName=sidebar
%5CPhlebtomyGuidelinesandOrderofdraw%20101017-2.pdf

3. Why should blood culture be first compared other tests?

Blood cultures are suspects you may have a blood infection. It’s important to test for
blood infections before any other tests because they can lead to serious complications.
Blood culture tubes are as a rule at the highest point of the request for attract to evade
defilement of the blood gathered. This is on the grounds that microorganisms from the
non-sterile cylinder plugs can bring about microbes development, which will appear in
the test as a blood disease. This is the reason blood culture bottles must be gathered
first before some other tubes.

https://www.healthline.com/health/blood-culture#purpose

https://laurenlovato.weebly.com/order-of-draw.html

4. What are other special considerations in the order of draw? Enumerate all and
explain.
 Royal Blue top tubes are
*specially prepared to provide high quality samples suitable for trace
element, toxicology and nutritional chemist determinations.
* may or may not contain an anticoagulant (ie. heparin, EDTA). You must
read the label to know what, if any additive is present.
* more expensive, and not often encountered.
 When collecting a coagulation test (light blue stoppered tube) using a ‘Butterfly’,
you must use a ‘discard’ tube (clear or light blue tube) first so that the sample
tube will not be under filled.
 CBC and Retic count (hematology tests) can BOTH be done on the same tube
lavender tube of blood.
 Prothrombin time (PT) and Partial Thromboplastin Time (PTT) (coagulation
tests) can BOTH be done on same light
  blue tube.
 o Glucose and Amylase (chemistry tests) can be done on same red OR green
tube.
(AUSTINCC,2016) https://www.austincc.edu/mlt/phb/Considerations%20in%20Blood
%20Collection%20_%20Spring2016_Dec815.pdf?
fbclid=lwAR1JqcpDZ5IUS3J1LIKOV1hKOV1hK0CfP4nmMGE1cVSImAGQe_3n3E8lyigmwcEg
5. Enumerate, Illustrate & differentiate the different winged- infusion sets.

Transferring Blood into Vacuum Collection Tubes from Syringe.

. Great care must be taken when transferring blood from the

syringe into the appropriate tubes. Safety devices must be used
which allow blood to safely be transferred from the syringe into the
appropriate tubes. After activation of the safety needle the needle is
removed and the transfer device is attached to the syringe. The old
method of directly piercing the stopper with the needle to transfer
the blood is no longer recommended. This method required that the
tube be placed in a rack and then, using one hand, carefully seat the
needle into the stopper, the other hand can then safely hold the
tube while the stopper is punctured, the blood would then be pulled into the tube by the vacuum. This
is a dangerous method as many phlebotomists are tempted to either hold the tubes in the hand while
piercing the stopper or, if the tube is in a rack, holding the tube with their free hand while piercing the
stopper. If the phlebotomist missed the stopper a needle stick exposure will likely occur.

Venipuncture Butterfly Using Vacuum Collection Tubes

The butterfly comes with an adapter to be used for vacuum

tube collection. This should RARELY be used. If a vein is large
enough to be used for a butterfly/vacuum collection then a regular
vacuum collection holder/needle assembly should be used. An
exception for this would be a large hand vein. Drawing blood from
a hand vein using a holder/needle assembly is very awkward. Also
the smaller needle is less painful in the hand veins. Be aware that if
the butterfly is used with vacuum collection tubes the vacuum in
the tubes will cause small veins to collapse. The butterfly=s primary
use is for a syringe draw where the amount of vacuum can be controlled by gentle pulling on the
plunger of the syringe.

http://phlebotomygeeks.yolasite.com/resources/GEEKS%20LAB%206%20BUTERFLY.pdf

6.