REFERENCES

Abde Kader and Amr, M. 2011.” Studying the efficiency of grey water treatment by using
rotating biological contactors system”, Fifteenth International Water Technology
Conference, IWTC-15, Alexandria, Egypt.
Abida Begum and Harikrishna, 2008. “Study on the Quality of Water in Some Streams of
Cauvery River,” Journal of Chemistry.
Abramovitz, J.N., 1996. Imperiled waters, impoverished future: The decline of freshwater
ecosystems. Worldwatch Paper 128. Worldwatch Insitute, Washington, DC.
Adeline, S.M., Chua., Tabateke, H., Satou, H and Mino, T., 2003. Production of PHA by
activated sludge treating Municipal waste-water. Effect of pH, sludge retention time
(SRT), and acetate concentration in influent. Water Research. 37(15): 3602- 3611.
Ahmad Mohammed Gumel., Mohamad Suffian Mohamad Annuar and Thorsten Heidelberg,
2012, Biosynthesis and Characterization of Polyhydroxyalkanoates Copolymers
Produced by Pseudomonas putida Bet001 Isolated from Palm Oil Mill Effluent, Article
PLoS 7(9) e45214.
Ahn, T.S., Hong, S.H., Kim, O.S., Yoon, J.J., Jeon, S.O and Choi, S.I 2001. The changes of
Bacillus spp. in municipal wastewater treatment plant with B3 process. Kor. J. Microbiol.,
37: 209 -213.
Ahn, W.S., Park, S.J and Lee, S.Y., 2001. Production of poly(3- hydroxybutyrate) from whey by
cell recycle fed-batch culture of recombinant E. coli. Biotechnol. Lett., 23 (3): 235-240.
Akar,. A, Akkaya, E.U., Yesiladali, S.K., Celikyilmaz, G., Cokgor, E. U., Tamerler, C., Orhon,
D and Cakar, Z. P., 2006. Accumulation of polyhydroxyalkanoates by Microlunatus
phosphovorus under various growth conditions, J. Ind. Microbiol. Biotechnol. 33 (3):
215 - 20.
Akcil, A., 2003.Destruction of Cyanide in Gold Mill Effluents: Biological versus Chemical
Treatments. Biotechnology Advances. 21 (6): 501 - 511.
Akiyama, M., Tsuge, T and Doi, Y., 2003. Environmental life cycle comparison of
polyhydroxyalkanoates produced from renewable carbon resources by bacterial
fermentation. Polym Degrad Stab. 80:183–194.
Alaerts, G.J., Rahman Mahbubar, M.D and Kelderman, P 1996. Performance analysis of a
fullscale duckweed covered sewage lagoon. Water Resources Development, 30 (4): 843 -
852.
Alias, Z and Tan, I.K.P., 2005. Isolation of palm oil-utilising polyhydroxyalkanoate (PHA)-
producing bacteria by an enrichment technique, Bioresource Technology, 96:1229 -
1234.
Alicia van der Merwe, 2008. Detachment of single and multi species bacterial biofilms by
crude enzymes extracted from wastewater biofilm and bacteria.
Allison, M. J., 1973. Population control in the rumen and microbial adaptation to dietary change,
In Weinberg and Sheffner (ed.), Buffers in ruminant physiology and metabolism. Church and
Dwight Co., Inc., New York: 10 - 18.
Aloice W. Mayo and Tatsuya Noike., 1996. Effects of temperature and pH on the growth of
heterotrophic bacteria in waste stabilization ponds, Japan Water Research (Impact
Factor: 4.66).

Altschul, S.F., Madden, T.L., Schaffer, A.A., Zhang, J., Zhang, Z., Miller, W and Lipman,
D.J. 1997. Gapped BLAST and PSI-BLAST: a new generation of protection database
search programmes. Nucleic Acid Research 25:3389-3402.
Amass, W., Amass, A and Tighe, B., 1998. A review of biodegradable polymers: Uses,
current developments in the synthesis and characterization of biodegradable
polyesters, blends of biodegradable polymers and recent advances in biodegradation
studies. Polym. Int. 47: 89 - 144.
American Public Health Association (APHA), 1989.American Water Works Association and
Water Pollution Control Federation Standard methods for the examination of water
and wastewater. American Water Works Association and Water Environment
Federation. 17th Edition. APHA, Washington D.
American Public Health Association (APHA), 1992. Standard Methods for Examination of
wastewater. 18th ed., American Public Health Association, American Water Works
Association, Water Environmental Federation, Washington, DC.
Anderson, A. J and Dawes, E. A., 1990. Occurrence, Metabolism, Metabolic Role, and Industrial
Uses of Bacterial Polyhydroxyalkanoates. Microbiological Reviews. 54 (4): 450-472.
Andrea Legat., Claudia Gruber., Klaus Zangger., Gerhard Wanner and Helga Stan-Lotter.,
2010. Identification of polyhydroxyalkanoates in Halococcus and other haloarchaeal
species, Appl Microbiol Biotechnol. 87(3): 1119–1127.
Aneja K. R., 2005. Experiments in Microbiology, Plant Pathology and Biotechnology: IVth
edition, New Age International (p) limited, publisher, New Delhi.
Annarita Poli., Paola Di Donato., Gennaro Roberto Abbamondi and Barbara Nicolaus., 2011.
Synthesis, Production, and Biotechnological Applications of Exopolysaccharides and
Polyhydroxyalkanoates by Archaea, Hindawi Publishing Corporation Archaea, 2011,
13 pages.

Anthony Paladino, 2009., Screening, Optimization and Extraction of Polyhydroxyalkanoates

and Peptidoglycan from Bacillus megaterium, Dissertations, master's theses and
master's reports.497.
Antonie, R .L and Chalmers, A .1970. Response of the Biodisc Process to Fluctuating
Wastewater Flows . Engr . Bulletin of Purdue Univ. 137: 427.
Antonie, R.L and Welch, F.M., 1969. "Preliminary Results of a Novel Biological Process for
Treating Dairy Wastes." Engr . Bulletin of Purdue Univ.135:115 - 126.
Antony, V., Samrot., Reddy, B., Avinesh., Sahaya, D., Sukeetha and Senthil Kumar, P., 2011.
Accumulation of poly [(R) – 3 hydroxyalkanoates] in Enterobacter cloacae SU – I
during growth with two different carbon sources in batch culture, Appl. Biochem
Biotechnol 163: 195.
Apilanez, I., Gutierrez, A and Diaz, M., 1998. Effect of surface materials on initial biofilm
development. Bioresource Technology 66 (3): 225-230.
Arai, Y., Nakashita, H., Suzuki, Y., Kobayashi, Y., Shimizu, T., Yasuda, M., Doi, Y and
Yamaguchi, I., 2002. Synthesis of a novel class of polyhydroxyalkanoates in Arabidopsis
peroxisomes, and their use in monitoring short-chain-length intermediates of
betaoxidation. Plant Cell Physiol.43:555-562.
Aravind Jeyaseelan, Sasikala Pandiyan and Preethi Ravi., 2012. Production of
polyhydroxyalkanoate (pha) using hydrolyzed grass and syzygium cumini seed as low
cost substrates, Journal of Food Sciences Microbiology,Biotechnology and Food
Sciences, 2 (3) 970-982.
Arnold, L., Demain, J and Davis, E., 1999. Polyhydroxyalkanoates. Manual of Microbiol. And
Biotech. 2: 616 – 627.
Arun, A., Arthi, R., Shanmugabalaji, Vand Eyini, M., 2009. Microbial production of poly-P-
hydroxybutyrate by marine microbes isolates from various marine environments.
Bioresour. Technol. 100: 2320-2323.
Ashby, R.D., Solaiman, D.K.Y., Foglia, T.A and Liu, C.K., 2001. Glucose/lipid mixed substrates
as a means of controlling the properties of medium chain length
poly(hydroxyalkanoates). Biomacromolecules 2(1): 211–216.
Ashfaq Ahmed Pathan, Rasool Bux Mahar, and Kamran Ansari, 2011. “Preliminary Study of
Grey water Treatment through Rotating Biological Contactor”, Mehran university
research journal of engineering & technology (30).
Ashton, P.J., 2002. Avoiding conflicts over Africa’s water resources. Ambio 31(3): 236-242.
Ashton, P.J., 2007. Riverine biodiversity conservation in South Africa: current situation and
future prospects. Aquatic Conservation: Marine Freshwater Ecosystems 17: 44-445.
Attia Razzaq., Nazia Jamil., Nighat Naheed and Shahida Hasnain., 2010. Bacteria from
contaminated urban and hilly areas as a source of polyhydroxyalkanoates production,
African Journal of Biotechnology. 9 (13): 1919-1925.

Augustinus Ignatius Kristijanto, Widhi Handayani, Purwanti Asih Anna Levi. 2011, The
Effectiveness of Anaerobic Baffled Reactor And Rotating Biological Contactor In Batik
Wastewater Treatment, Makara Journal of Technology, (15) 2.

Avasan Maruthi, Y and Ramakrishna S. Rao. 2001. Effect of sugar mill effluent on organic
resources of fish. Poll. Res. (20) 2: 167- 171.
Ayub, N.D., Pettinari, M.J., Ruiz, J.A and Nancy, I.L., 2004. A polyhydroxybutyrateproducing
Pseudomonas sp. isolated from antarctic environments with high stress resistance. Curr.
Microbiol. 49: 170-174.
Babel, W., Ackermann, J.U and Breuer, U., 2001. Physiology, regulation, and limits of the
synthesis of poly (3HB). In Advances in Biochemical Engineering/Biotechnology:
Biopolyesters, 71: 125–157. Scheper, T., Babel, W. and Steinbuchel, A. Berlin,
Heildelberg: Springer Press.
Bahman Yargholi and Nasim Jafari, 2011. Application of Rotating Biological Contactor with
Packing Bed (RBCp) for Small Communities Wastewater Treatment to Be Used in
Irrigation, International Journal of Water Resources and Arid Environments (1)2: 102-
109
Banerjee, G., 1998. Hydraulics of Bench Scale Rotating Biological Contactor. Water
Research Environ. Health. 40(3): 253-260.
Barbuzzi, T., Giuffrida, M., Impallomeni, G., Carnazza, S., Ferreri, A., Guglielmino, S.P.P and
Ballistreri, A., 2004. “Microbial synthesis of poly(3-hydroxyalkanoates) by Pseudomonas
aeruginosa from fatty acids: identification of higher monomer units and structural
characterization” Biomacromolecules.5 (6): 2469-2478.
Bascomb, S., Lapage, S. P., Wilcox, W. R and Curtis, M. A. 1971. Numerical classification of
the tribe Klebsiellae. Journal of General Microbiology 66:279–295.
Bauer, A.W., Kirby, W.M.M., Sherris, J.C and Turck, M. 1966. Antibiotic susceptibility Testing
by a standardized Single disc method, The American journal of clinical pathology, 36: 493 –
496.
Belova, L.L., Sokolov, A.P., Morgunov, I.G and Trotsenko Yu, A., 1997. Purification and
characterization of citrate synthase from Methylobacterium extorquens-a methylotrophic
producer of polyhydroxybutyrate. Biochemistry 62: 71-6.
Benefield, L.D and Randall, C.W., 1980. Biological Process Design for Wastewater Treatment,
Prentice Hall, New York, U.S.A.
Bengtsson, S., Werker, A., Christensson, M and Welander, T., 2008. Production of
polyhydroxyalkanoates by activated sludge treating a paper mill wastewater. Bioresour.
Technol., 99(3): 509-516.
Bennet, J. 2003. It’s all in the bag: will UK introduce a tax on the plastic carrier bags. Materials
Recycling Week. 181(3): 15-17.
Berger, E., Ramsey, B.A., Ramsay, J.A., Chavarie, C and Braunegg, G.1989. PHB recovery by
hypochlorite digestion of non-PHB biomass. Biotechnol. Tech. 3: 227-232.
Bergey, D. H., Harrison, F. C., Breed, R. S., Hammer, B. W and Huntoon, F. M. 1923. Bergey’s
manual of determinative bacteriology. 1st ed. Baltimore, Williams and Wilkins.
Berlanga, M., Montero, M.T., Fernandez Borrell, J and Guerrero, R. 2006. Rapid
spectrofluorometric screening of polyhydroxyalkanoate producing bacteria from
microbial mats, International Microbiology, 9: 95 – 102.
Bertrand, J.L., Ramsay, B.A., Ramsay, J.A and Chavarie, C., 1990. Biosynthesis of poly-
ßhydroxyalkanoates from pentoses by Pseudomonas pseudoflava. Appl. Environ.
Microbiol. 56(10): 3133–3138.
Bevan, 1971. The disposal of sugar mills effluents in Queensland 40th proceeding of the
T.S.S.C.T. Louisinia October November, PP 150.
Bintanja, H.H.J., van der Erve, J.J.V.M., Boelhouwer, C.1975. Oxygen Transfer in a Rotating
Disc Treatment Plant, Water Research 9 (12): 1147–1153.

Bishop, P., Wuertz, S and P. Wilderer 2003. The effect of biofilm heterogeneity on metabolic
processes, In: Biofilms in Wastewater Treatment, An interdisciplinary Approach, Bishop,
Wuertz and Wilderer (Ed). IWA publishing House, UK, PP: 125.
Bitton, G., 2005. Wastewater Microbiology, 3 ed. Wiley – Liss, John Wiley and Sons, inc.
New Jersey, USA:766.

Bohmert,K., Balbo, I., Tischendorf, G., Steinbuchel, A and Willmitzer, L.2002. Constitutive
expression of the 0- ketothiolase gene in transgenic plants-A major obstacle for obtaining
polyhydroxybutyrate-producing plants. Plant Physiol. 128, 1282-1290.
Boller, M., Gujer, W and Nyhuis, G., 1990. Tertiary rotating biological contactors for
nitrification. Water Science and Tech. 22(1/2): 89 - 100.
Bonatto, D., Matias, F., Lisboa, M.P., Bogdawa, H.M and Henriques, J.A.P., 2004. Production
of short side chain-poly[hydroxyalkanoate] by a newly isolated Ralstonia pickettii strain.
World J. Microbiol. Biotechnol. 20: 395-403.
Boopathy, R., 2000, Factor limiting bioremediation technologies. Bioresource Technology, 74:
63-67.
Boramann, E. J., Leisnner, M., Roth, M., Beer, B and Metzner K., 1998. Production of
polyhydroxybutyrate by Ralstonia eutropha from protein Hydrolysates, Applied
environmental microbiology. 50: 604 - 607.
Bourque, P.Y., Pomerleau, Y and Groleau, D., 1995. High cell-density production of
polyhydroxybutyrate (PHB) from methanol by Methylobacterium extorquens: Production
of high-molecular-mass PHB, Applied Microbial Biotechnology. 44 (3 – 4): 367 - 376.
Bozel, J., 2001. Chemicals and Materials from Renewable Resources, Proceedings of a
Symposium held at the 218th National Meeting of the ACS in New Orleans, Louisiana,
ACS Symp. Ser. 784, ACS, Washington D. C, USA: 226.
Bramucci, M.G and Nagarajan, V., 2000. Industrial wastewater bioreactors: sources of novel
microorganisms for biotechnology. TIBTECH 18: 501 – 505.
Brandl, H., Gross, R.A., Lenz, R.W and Fuller, R.C., 1988: Pseudomonas oleovorans as a source
of poly(hydroxyalkanoates) for potential applications as biodegradable polyesters. Appl.
Environ. Microbiol. 54 (8): 1977-1982.
Braunegg, G., Lefebvre, G and Genser, K.F., 1998. Polyhydroxyalkanoates, biopolyesters from
renewable resources: Physiological and engineering aspects. J Biotechnol, 65 (2 – 3):
127–161.
Braunegg, G., Lefebvre, G., Renner, G., Zeiser, A., Haage, G and Loidl-Lanthaler, K., 1995.
Kinetics as a tool for polyhydroxyalkanoate production optimization. Can. J. Microbiol.
41(1): 239–248.
Brock, T.D., 1969. Microbial growth under extreme conditions, Symp. Soc. Gen. Microbiol. 19:
15 –41.
Bryan, E.H and Moeller, D.H. 1960. Aerobic Biological Oxidation Using Dowpac, Paper 42,
Conference on Biological Waste Treatment, Manhattan College, April 20.
Bryers, J.D. and Characklis, W.G 1990. Biofilms in water and wastewater treatment. In:
Biofilms, Characklis, Marshall, (Ed.) John Wiley & Sons, New York, PP: 671, 1990.

Buranasilp ,K and Charoenpanich, J. 2011. Biodegradation of acrylamide by Enterobacter

aerogenes isolated from wastewater in Thailand, J Environ Sci, China, 23(3): 396–403.

Burns, D .G., Peter H. Janssen., Takashi Itoh., Masahiro Kamekura., Zhuo Li., Grant Jensen.,
Francisco Rodriguez-Valera., Henk Bolhuis and Mike, L., 2007. Dyall-Smith,
Haloquadratum walsby gen. nov., sp. nov., the square haloarchaeon 31 of Walsby,
isolated from saltern crystallizers in Australia and Spain, Int. J. Syst. Evol. Microbiol. 57:
387 - 392.
Byrom, D., 1987. Polymer synthesis by microorganisms: Technology and economics. Trends
Biotechnol. 5 (9): 246-250.
Byrom, D., 1992. Production of Poly-β-hydroxybutyrate: Poly- β -hydroxyvalerate Copolymers,
Microbiology Reviews. 103: 247-250.
Cappa, F and Cocconcelli, P.S. 2001. Identification of fungi from dairy products by means of
18S rRNA analysis. Int. J. Food Microbiol. 69:157–160.
Castellani, A., Chalmers, A. J. 1920. Sur la classification de certains groupes de bacilles aerobies
de l’intestin humain. Annales de l’Institut Pasteur 34:600–621.
CEFIC/CEEP. 1988a. Phosphates: a sustainable future in recycling. CEFIC, Belgium.

CEFIC/CEEP. 1988b. International conference on Recovery of phosphates for recycling from

sewage and animal wastes; summary of conclusions and discussions. CEFIC, Belgium

Chamie Joseph., 2004. Bulletin of the International Statistical Institute 54th Session Book 1:159
– 184.
Chan, P.L., Yu, V., Wai, L., Yu, H.F. 2006. Production of medium-chain-length
polyhydroxyalkanoates by Pseudomonas aeruginosa with fatty acids and alternative
carbon sources. Curr. Med.Chem.13 : 377 – 398.
Chandra, R and Rustgi, R.1998. Biodegradable Polymers. Progress in polymer science. 23:1273-
1335.
Characklis, W.G., McFeters, G.A and Marshall, K.C (1990a) Physiological ecology of biofilm
systems. In: Characklis WG, Marshall KC (eds) Biofilms. Wiley, New York, pp 341–393.
Characklis, W.G., Turakhia, M.H and N, Zelver (1990b) Transfer and interfacial transport
phenomena. In: Characklis WG, Marshall KC (eds) Biofilms. Wiley, New York, pp 265–
340.
Chaudhry, W. N., Jamil, N., Ali, I., Ayaz, M. H and Hasnain, S., 2011. Screening for
polyhydroxyalkanoate (PHA)-producing bacterial strains and comparison of PHA
production from various inexpensive carbon sources. Ann. Microbiol: 61: 623–629.

Chen, D., Han, Y and Gu, Z., 2006. Application of statistical methodology to the optimization
of fermentative medium for carotenoids by Rhodobacter sphaeroides, Process
Biochemistry. 41(8): 1773 - 1778.
Chen, G.Q and Wu, Q. 2005. The application of polyhydroxyalkanoates as tissue engineering
materials. Biomaterials.26(33):6565-78.
Chen, S., Coffin, D. E and Malone, R. F. 1993. Production, characteristics, and modeling of
aquacultural sludge from a recirculating aquacultural system using a granular media
biofilter, J.K. Wang, editor. Techniques for modern aquaculture. American Society of
Agricultural Engineers, St. Joseph, Michigan.
Chenyu Du, Julia Sabirova, Wim Soetaert, Sze Ki Carol Lin, 2012, Polyhydroxyalkanoates
Production From Low-cost Sustainable Raw Materials, Current Chemical Biology, 6 (1):
14 – 25.
Chien, C.C., Chen, C.C., Choi, M.H., Kung, S.S and Wei, Y.H., 2007. Production of
polyhydroxybutyrate (PHB) by Vibrio spp. isolated from marine environment. J.
Biotechnol. 132(3):259-63.
Cho, K.S., Ryu, H.W., Park, C.H and Goodrich, P.R., 1997. Poly(hydroxy-butyrate- co-
hydroxyvalerate) from swine waste liquor by Azotobacter vinelandii UWD. Biotechnol.
Lett. 19: 7-10.
Choi, G.G., Kim, H.W and Rhee, Y.H., 2004. Enzymatic and non-enzymatic degradation of poly
(3 -hydroxybutyrate- co-3-hydroxyvalerate) copolyesters produced by Alcaligenes sp
MT-16. J. Microbiol. 42 (4): 346-352.
Choi, J and Lee, S.Y., 1999. Factors affecting the economics of polyhydroxyalkanoate
production by bacterial fermentation, Appl. Microbiol. Biotechnol., 51(1):13-21.
Choi, M.H and Yoon, S.C., 1994. Polyester biosynthesis characteristics of Pseudomonas
citronellosis grown on various carbon sources, including 3methyl- branched substrates.
Appl. Environ. Microbiol. 60(9): 3245-3254.
Chua, H., Yu, P. H and Ho, L.Y., 1997. Coupling of waste water treatment with storage
polymer production, Appl. Biochem. Biotechnol. Spring; 63-65:627-635.
Chuang, S. H., Ouyang, C. F., Yuang, H. C and You, S. J., 1998. Phosphorus and
Polyhydroxyalkanoates variation in a combined Process with activated sludge and
biofilm, Water Sci. Tech. 37 (4-5): 593–597.
Clark, J.H., Moseng, E.M and Asano, T., 1978. Performance of a rotating biological contactor
under varying wastewater flow. J. Water Pollut. Control Fed. 50 (5): 896–911.
Collins, S., 1987. Choice of substrate in polyhydroxybutyrate synthesis. Spec. Publ. Soc.
General Microbiol., 21: 161-168.
Cowan, M.M., Warren, T.M and Fletcher, M. 1991. Mixed species colonization of solid surfaces
in laboratory biofilms. Biofouling 3: 23–34.
Cripps, S.J., 1991. Comparison of methods for the removal of suspended particles from
aquaculture effluents, Aquaculture and the environment: short communications and
abstracts presented at the International Conference Aquaculture Europe ’91, Dublin,
Ireland, European Aquaculture Society, Bredene, Belgium.
Crites, R. and Tchobanoglous, G. 1998. Small and Decentralized Wastewater Management
Systems, The McGraw-Hill Companies. New York.
Crocetti, G .R., Banfield, J .F., Keller, J., Bond, P .L and Blackall, L .L.2002. Glycogen-
accumulating organisms in laboratory-scale and full-scale wastewater treatment
processes. Microbiology 148(Pt 11), PP: 3353 - 64.
Cull, S.G., Holbrey, J.D., Vargas-Mora, V., Seddon, K.R and Lye, G.J., 2000. Room-temperature
ionic liquids as replacements for organic solvents in multiphase Bioprocess Operations.
Biotechnol. Bioeng. 69 (2): 227-233.
Cynthia Q Sun., Charmian J O' Connor., Susan J Turner., Gillian D Lewis., Stanley, R. A and
Anthony M. Roberton., 1998. The effect of pH on the inhibition of bacterial growth by
physiological concentrations of butyric acid: Implications for neonates fed on suckled
milk, Chemico-Biological Interactions, Elsivier, 113 (2): 117–131.
Dahllof, I., Baillie, H and Kjelleberg, S. 2000. rpoB-based microbial community analysis avoids
limitations inherent in 16S rRNA gene intraspecies heterogeneity. Appl Environ Microb 66
(8): 3376–3380.
Dalal, J., Sarma, P. M., Lavania, M., Mandal, A. K and Lal, B., 2010. Evaluation of bacterial
strains isolated from oil-contaminated soil for production of polyhydroxyalkanoic acids
(PHA), Pedobiologia. 54 (1): 25-30.
Dallas, H. F. and Day, J. A. 2004.The effect of water quality variables on aquatic ecosystems:
a Review, Water Research Commission. Pretoria, South Africa.
Dallinger R., Prosi, F., Segner, H and Back, H. 1987. Contaminated food and uptake of heavy
metals by fish: a review and a proposal for further research.
Daniel, M., Chori, J.H., Kim, J.H and Lebeault, J.M., 1992. Effect of nutrient deficiency on
accumulation and relative molecular weight of poly-3-hydroxybutyric acid by
methylotrophic bacterium, Pseudomonas 135.Appl. Microbiol. Biotechnol. 37: 702–
706.

Darshan Marjadi and Nishith Dharaiya., 2011. Isolation, screening and characterization of
polyhydroxyalkanoates producing bacteria utilizing edible oil as carbon source,
Journal of Environmental research and development. 5 (3A): 764 – 772.

Dave, H., Ramakrishna, C and Desai, J.D., 1996. Production of polyhydroxybutyrate by

petrochemical activated sludge and Bacillus sp. IPCB-403. Indian J. Experimental
Biology. 34 (3): 216-219.
David, A., 2005. Experimental studies of bacterial Interactions with aqueous heavy metal
Cations and mineral surfaces.
Davies, B and Day, J., 1998. Vanishing Waters. UCT Press (First Edition). Chapter 4: 79-84 and
Chapter 9: 281.
Dawes, E. A and Senior, P. J., 1973. The Role and Regulation of Energy Reserve Polymers in
Micro-organisms. Advances in Microbial Physiology. 10: 135-266.
Dawes, E. A. 1985. Starvation, Survival and Energy Reserves, In: Bacteria in Their Natural
Environment, Fletcher, M. and Floodgate, G. D. (eds.), Academic Press, Florida: 43 -
79.
Dawes, E.A and Senior, P.J., 1973. The role and regulation of energy reserve polymers in micro-
organisms, Adv Microb. Physiol. 10: 135 - 266.
De Koning G. J. M., 1995. Physical Properties of Bacterial Poly ((R)-3-hydroxyalkanoates),
Can. J. Microbiol, 41(1): 303 - 309.
De Koning, G. J. M. and Lemstra, P.J. 1992. The amorphous state of bacterial poly [(R)-3-
hydroxyalkanoate] in vivo. Polymer, 33(15): 3292-3294.

de Koning, G.J.M., Kellerhals, M., van Meurs, C and Witholt ,B., 1997. A process for the
recovery of poly(hydroxyalkanoates) from Pseudomonads. Part 2: process, development
and economic evaluation. Bioprocess Eng. 17 (1): 15–21.
de Smet, M. J., Eggink, G., Witholt, B., Kingma, J., Wynberg, H., 1983. Characterization of
intracellular inclusions formed by Pseudomonas oleovorans during growth on octane. J.
Bacteriol. 154 (2): 870-878.
Deinema, M.H., 1972. Bacterial flocculation and production of poly-betahydroxybutyrate.
Appl. Microbiol. 24(6): 857-8.
Delafield, F. P., Doudoroff, M., Palleroni, N. J., Lusty, C. J and Contopoulos, R., 1965.
Decomposition of poly-13-hydroxybutyrate by soil pseudomonas. J. Bacteriol. 90: 1455 -
1466.
Delisle, C.E and Schmidt, J.W.,1977. The effects of sulphur on water and aquatic life in Canada.
In: Sulphur and its inorganic derivatives in the Canadian environment. Ottawa, Ontario,
National Research Council of Canada (NRCC No. 15015).

Dennis, D., Liebig, C., Holley, T., Thomas, K.S., Khosla, A., Wilson, D and Augustine, B. H.
2003. Preliminary analysis of polyhydroxyalkanoate inclusions using atomic force
microscopy. FEMS Microbiol. Lett., 226:113 - 119.

Dennis, D., Sein, V., Martinez, E and Augustine, B. H. 2008. PhaP Is Involved in the
Formation of a Network on the Surface of Polyhydroxyalkanoate Inclusions in
Cupriavidus necator H16 . J. Bacteriol., 190 (2): 555 - 563.
Department of National Health and Welfare (Canada), 1978. Guidelines for Canadian drinking
Doan, Thuoc Van, Nguyen and Binh Thi, 2012. Polyhydroxyalkanoates production by a
bacterium isolated from mangrove soil samples collected from Quang Ninh province,
J. Viet. Env. 3 (2): 76 - 79.
Doi, Y., Kunioka, M., Nakamura, Y and Sago, K., 1987. Biosynthesis of Copolyesters in
Alcaligenes eutrophus H16 from 13C-Labeled Acetate and Propionate. Macromolecules.
20: 2988-2991.
Doi, Y., Segawa, A., Nakamura, S and Kunioka, M., 1990. Production of biodegradable
Copolymers by Alcaligenes eutrophus. In: Novel Biodegradable Microbial Polymers,
Dawes, E. A. (ed.), Kluwer Academic Publishers, The Netherlands, 37-48.
Doi, Y., Tamaki, A., Kunioka, M and Sogo, K., 1988. Production of copolyesters of 3-
hydroxybutyrate and 3-hydroxyvalerate by Alcaligenes eutrophus from butyric and
pentanoic acids. Applied Microbiology and Biotechnology. 28: 330-334.
Doi, Y.,1990. Microbial Polyesters. VCH Publishers, Inc. New York, USA.

Doi, Yand Abe, C. 1990. Biosynthesis and characterization of a new bacterial copolyester of 3-
hydroxyalkanoates and 3-hydroxy-ω-chloroalkanoates. Macromolecules 23: 3705-3707.
Donlan, R.M., Pipes, W.O and Yohe, T.L. 1994. Biofilm formation on cast iron substrata in
water distribution systems. Wat. Res. 28 (6): 1497 - 1503.
Dragon, D. C and Rennie, R.P. 1995. The ecology of anthrax spores: tough but not invincible,
The Canadian Veterinary Journal 36 (5): 295 - 301.
Drancourt, M., C, Bollet., A, Carlioz., R, Martelin., Gayral, J.P and Raoult, D. 2000. 16S
ribosomal DNA sequence analysis of a large collection of environmental and clinical
unidentifiable bacterial isolates, J Clin. Microbiol. 38(10):3623-30.
Du, G., Chen, J., Yu, J and Lun, S., 2001. Continuous production of poly-3-hydroxybutyrate by
Ralstonia eutropha in a two stage culture system. J. Biotech., 88 (1): 59-65.
Du, G.C., Chen, J., Gao, H.J., Chen, Y.G and Lun, S.Y., 2000. Effects of environmental
conditions on cell growth and poly-β-hydroxybutyrate accumulation in Alcaligenes
eutrophus. World J. Microbiol. Biotechnol. 16: 9-13.
Dugan, G. L.1984. Rotating biological contactor for brackish wastewater effluent treatment.
Honolulu (HI): Water Resources Research Center, University of Hawaii at Manoa.
WRRC special report, (3)12:84.
Durner, R., Witholt, B and Egli, T., 2000. Accumulation of poly [(R)-3-Hydroxyalkanoates] in
Pseudomonas oleovorans during growth with octanoate in continous culture at different
dilution rates. Applied and Environmental Microbiology. 66(8), 3408-3414.
DWAF - (Department of Water Affairs and Forestry), 1996a. South African water quality
guidelines (1): Domestic use. Department of Water Affairs and Forestry. Pretoria,
South Africa.
DWAF - (Department of Water Affairs and Forestry), 1996b. South African water quality
guidelines (7): Aquatic ecosystems. Department of Water Affairs and Forestry.
Pretoria, South Africa.
Ebrahimi, A., Asadi, M and Najafpour, G.D., 2008. Dairy Wastewater Treatment Using Three
Stage Rotating Biological Contactor”, IJE TRANSACTIONS B 22 (2): 107 - 114.
Ebtesam El-Bestawy, Mohamed H. El-Masry, Nawal E. El-Adl, 2005. The Potentiality of
Free Gram-negative Bacteria for Removing Oil and Grease from Contaminated Industrial
Effluents, World Journal of Microbiology and Biotechnology 21(6-7):815-822.

Eckenfelder, W.W.J., 1989. Industrial Water Pollution Control. McGraw-Hill, New York.
Edwards, P. R and Mary, A. 1955. Studies on the Klebsiella- Aerobacter group of bacteria.
Journal of Bacteriology 70(4): 382 - 390.
Edwards, U., Rogall, T., Bocker, H., Emde, M and Bottger, E., 1989. Isolation and direct
complete nucleotide determination of entire genes. Characterization of a gene coding
for 16S ribosomal DNA. Nucleic Acids Res.17:7843-53.
Eickhoff, T. C., Steinhauer ,B. W and Finland M. 1966. The Klebsiella-Enterobacter-Serratia
division: Biochemical and serologic characteristics and susceptibility to antibiotics. Annals
of Internal Medicine 65(6): 1163 -1179.
Elkelboom, D.H and Geurkink, B. 2002. Filamentous micro-organisms observed in industrial
activated sludge plants. Water Science and Technology 46(1-2): 535 542.
Ellar, D., Lundgren, D. G., Okamura, K and Marchessault, R. H. 1968. J. Mol. Biol.35: 489 -
502.
Environ. Health, 40( 3): 253-260, 1998
Esterl, S., Hartmann, C.H and Delgado, A. 2003. In: Biofilms in Wastewater Treatment, An
interdisciplinary Approach, P. Bishop, S. Wuertz and P. Wilderer (Ed), IWA publishing
House, UK, PP: 88.
Eversloh, T.L., Bergander, K., Luftmann ,H and Steinbuchel, A. 2001. Identification of a new
class of biopolymer: bacterial synthesis of a sulfur-containing polymer with thioester
linkages. Microbiology. 147: 11-19.
fed-batch culture of Alcaligenes latus, a growth-associated PHB producer. Biotechnol
Bioeng. 50: 197–202.
Findlay, R. H and White, D. C. 1983. Polymeric hydroxyalkanoates from environmental samples
and Bacillus megaterium. Applied and Environmental Microbiology. 45 (1): 71-78.
Flechter, A.,1993. PHA as natural, biodegradable polyesters. In Plastics from bacteria and for
bacteria. Springer Verlag, New York: 77-93.
Fletcher, M. 1988. Attachment of Pseudomonas fluorecens to Glass and Influence of Electrolytes
on Bacterium-Substratum Separation distance. Journ of Bacteriology 170 (5): 2027–
2030.
Freddy Valdes, Edmundo Munoz, Rolando Chamy, Gonzalo Ruiz, Christian Vergara and
David Jeison, 2006. “Effect of sulphate concentration and sulphide desorption on the
combined removal of organic matter and sulphate from wastewaters using expanded
granular sludge bed (EGSB) reactors”, Electronic Journal of Biotechnology (9).
Fresenius, W., Schneider, W., Bohnke, B and Poppinghaus, K., 1989. (eds.), Waste Water
Technology: Origin, Collection, Treatment and Analysis of Waste Water. Springer-
Verlag, New York.

Fruhen, M., Christan, E., Gujer, W and Wanner, O., 1991. Significance of spatial distribution of
microbial species in mixed culture biofilms. Water Science and Technology. 23(7-9):
1365 – 1374.
Fuchtenbusch, B., Wullbrandt, D and Steinbuchel, A., 2000. Production of polyhydroxyalkanoic
acids by Ralstonia eutropha and Pseudomonas oleovorans from an oil remaining from
biotechnological rhamnose production. Appl Microbiol Biotechnol. 53:167–172
Fuh-Kwo Shiah and Hugh W. Ducklow, 1994.Temperature and substrate regulation of bacterial
abundance, production and specific growth rate in Chesapeake Bay, USA, P Marine
Ecology Progress Series. Mar. Ecol. Prog. Ser., 103: 297-308.
Fuhs, G.W and Chen, M. 1975. Microbiological basis of phosphate removal in the activated
sludge process for treatment of wastewater. Microbial Ecology 2 (2):119 - 138.
Fukui, T., Kichise, T., Yoshida, Y and Doi, Y., 1997. Biosynthesis of poly (3- hydroxybutyrate-
co-3-hydroxyvalerate-co-3-hydroxyheptanoate) polymers by recombinant Alcaligenes
eutrophus. Biotechnol Lett 19: 1093–1097.
Fux, C. and Siegrist, H. 2004. “Nitrogen removal from sludge digester liquids by
nitrification/denitrification or partial nitritation/anammox: environmental and
economical considerations”, Water Science and Technology (50), © IWA Publishing.
Garcia-Ribera, R., Monteoliva-Sanchez, M and Ramos-Cormenzana, A., 2001. Production of
polyhydroxyalkanoates by Pseudomonas Putida KT2442 harboring pSK2665 in
wastewater from olive oil mills (alpechin). Electronic Journal of Biotechnology.
4(2):116–119.
Gargi Goswami, Surabhi Chaudhuri and Debjani Dutta, 2007, Effect of pH and temperature on
pigment production from an isolated bacterium, Bioprocess and Biosystems Engineering,
35(8):1375-88.
Garland P. B, 1977, Energy transduction in microbial systems. Symp. Soc. Gen. Microbiol, 27,
1-21.
Garland, P. B., 1977. Energy transduction in microbial systems. Symp. Soc. Gen. Microbiol. 27:
1 - 21.
Garrity, M., Don J. Brenner, Noel R. Krieg and James T. Staley, 1984. Bergey's manual of
systematicbacteriology. The Proteobacteria. Williams and Wilkins. New York, USA.
Garrity, M., Don J. Brenner, Noel R. Krieg and James T. Staley, 2005. Bergey's manual of
systematicbacteriology. Volume Two: The Proteobacteria. Williams and Wilkins. New
York, USA.
Ghasem D. Najafpour, Punita Nook Naidu, and Azlina Harun Kamaruddin, 2005. “Rotating
Biological contactor for Biological Treatment of Poultry Processing Plant
Wastewater”. ASEAN Journal of Chemical Engineering (5): 2.
Gieseke, A., Lotte Bjerrum, Wagner, M and Amann, R. 2003. Structure and activity of multiple
nitrifying bacterial populations co-existing in a biofilm. Environ. Microbiol. (5), PP:355 -
369.
Godbole, S., Daginawala, H.F and Chakrabarti, T., 2000. Effect of carbon and nitrogen sources
on production of polyhydoxybutyrate from whey by Alcaligenes eutrophus, 59th Annual
Conference of Association of Microbologists of India: 59.
Goff, M., Ward, P.G., O'Connor, K.E., 2007. Improvement of the conversion of polystyrene to
polyhydroxyalkanoate through the manipulation of the microbial aspect of the process: a
nitrogen feeding strategy for bacterial cells in a stirred tank reactor. J. Biotechnol.,
132(3): 283- 286.
Government Gazette, 1984. Requirements for the purification of wastewater or effluent,
Gazette No. 9225, Regulation, 991.
Gowariker, V.R., Viswanathan, N.V and Jayadev Sreedharin, 1996.“Polymer Science”, tenth
reprint, New Age International (P) limited, Publishers, New Delhi.
Grage, K., Jahns, A.C., Parlane, N., Palanisamy, R., Rasiah, I.A., Atwood, J.A., et al.,2009.
Bacterial polyhydroxyalkanoate granules: biogenesis, structure and potential use as
 micro-/nano-beads in biotechnological and biomedical applications.
Biomacromolecules 10: 660–669.
Greenberg, A.E., Trussell, R.R and Clescri, L.S. 1985. Standard methods for the examination
of wastewater (16th edn). APHA, AWWA, WPCF, New York, USA.
Griebel, R., Smith, Z and Merrick, J. M. 1968. Metabolism of poly-beta-hydroxybutyrate. I.
Purification, composition, and properties of native poly-beta-hydroxybutyrate granules
from Bacillus megaterium. Biochemistry. 7(10): 3676 - 3681.

Griebel, R.J and Merrick, J.M., 1971. Metabolism of Poly-β-Hydroxybutyrate: Effect of Mild
Alkaline Extraction on Native Poly-β-Hydroxybutyrate Granules. J. Bacteriol.,
108(2): 782–789.

Grothe, E., Young, M.M and Chisti, Y., 1999. Fermentation optimization for the production of
poly (β-hydroxybutyric acid) microbial thermoplastic. Enzyme and Microbial
Technology. 25(1-2): 132 - 141.

Gu, J.D., 2003. Microbiological deterioration and degradation of synthetic polymeric materials:
recent research advances. Int. Biodeter. Biodegr. 52: 69– 91.
Guilmanov,V., Ballistreri, A ., Impallomeni, G and Richard A. Gross., 2002. Oxygen transfer
rate and sophorose lipid production by candida bombicola. Biotechnol Bioeng, 77:489 ±
494.
Gulhane M.L and Kaley A. A., 2014. Proceedings of 3rd IRF International Conference, Goa,
India.
Gupta Shraddha., Rajput Yogita., Shit Simanta., Shukla Aparna and Shukla Kamlesh, 2012.
Screening of polyhydroxyalkanoates (phas) Producing bacteria from rhizospheric soils
of Chhattisgarh, Adv. Pharmacol. Toxicol. 13 (3): 51 -56.
Gupta, A.B and Gupta, S.K., 1999. Simultaneous carbon and nitrogen removal in a mixed culture
aerobic RBC biofilm. Water Res. 33(2): 555-561.
Gupta, S., Banerjee, S., Saha, R., Datta, J. K and Mondal, N.2006. Fluoride geochemistry of
groundwater in Birbhum, West Bengal, India, Fluoride, (39), PP: 318.
Gursel, I and Hasirci, V., 1995. Properties and drug release behaviour of poly(3-
hydroxybutyric acid) and various poly(3-hydroxybutyrate-hydroxyvalerate) copolymer
microcapsules. J. Microencapsul, 12(2): 185–193.
Haba, E., Vidal-Mas, J., Bassas, M., Espuny, M.J., Llorens, J and Manresa, A., 2007. Poly 3-
(hydroxyalkanoates) produced from oily substrates by Pseudomonas aeruginosa 47T2
(NCBIM 40044): Effect of nutrients and incubation temperature on polymer composition,
Biochem. Engineering J. 35:99 - 106.
Hahn, J.J., Eschenlauer, A.C, Narrol, M.H., Somers, D.A and Srienc, F.1997. Growth kinetics,
nutrient uptake, and expression of the Alcaligenes eutrophus poly(B-hydroxybutyrate)
synthesis pathway in transgenic maize cell suspension cultures. Biotechnol. Prog. 13:
347-354.
Hahn, S.K., Chang, Y.K., Kim, B.S and Chang, H.N. 1994. Optimization of microbial poly(3-
hydroxybutyrate) recovery using dispersions of sodium hypochlorite solution and
chlorofonn. Biotechnol. Bioeng. 44: 256-261.
Hajrasuliha, S, 1979.Accumulation and toxicity of chloride in bean plants, Plant and Soil,
55(1).
Hall, T.A., 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis
program for Windows 95/98/NT. Nucleic Acids Symp. Ser. 41: 95-98.
Hall-Stoodley, L., Costerton, J. W and Stoodley, P. 2004. "Bacterial biofilms: from the
natural environment to infectious diseases", Nature Reviews Microbiology. 2(2):95-108.

Halweil, B. 2004. In ‘Behind the scenes: Plastic bags’. State of the world 2004. A world watch
Institute report on progress towards sustainable society. Edited by Linda Starke, W. W.
Norton and Company New York.
Handrick, R., Reinhardt, S and Jendrossek, D., 2000. Mobilization of poly(3hydroxybutyrate) in
Ralstonia eutropha. J. Bacteriol. 182 (20): 5916-5918.
Hanggi, U. J., 1995. Requirements on bacterial polyesters as future substitute for conventional
plastics for consumer goods, FEMS Microbial. Rev. 16: 213 - 220.
Hankermeyer, C.R and Tjeerdema, R.S.1999. Polyhydroxybutyrate: Plastic made and degraded
by Microorganisms. Reviews in Environmental contamination and Toxicology. 159:1-24.
Hartmann, R., Hany, R., Geiger, T., Egli, T., Witholt, B and Zinn, M. 2004. Tailored
biosynthesis of olefinic medium-chain-length poly[(R)-3-hydroxyalkanoates] with
improved thermal properties. Macromolecules 37 (18): 6780-6785.
Hassan, M.A., Nawata, O., Shirai, Y., Rahman, N.A.A., Yee, P.L., Bin Ariff, A., Ismail, M
and Karim, A., 2002. A proposal for zero emission from palm oil industry
 incorporating the production of polyhydroxyalkanoates from palm oil mill effluent. J
Chem Eng Jpn. 35: 9–14.

Hassan, M.A., Shirai, Y., Kusubayashi, N., Ismail abdul karim, M., Nakanishi, K and
Hashimoto, K., 1997.The production of polyhydroxyalkanoate from anaerobically palm
oil mill effluent by Rhodobacter sphaeroides, Journal of Fermentation and
Bioengineering 83(5): 485 -488.

Hauser Barbara A., 2001. Drinking water chemistry, A laboratory manual, Lewis publishers,
Florida USA.
Haywood, G. W., Anderson, A. J and Dawes, E. A., 1989. A survey of the accumulation of novel
polyhydroxyalkanoates by bacteria. Biotechnol. Lett. 11(7): 471- 476.
Haywood, G. W., Anderson, A.J and Dawes, E. A., 1989. The importance of PHB-synthase
substrate specificity in polyhydroxyalkanoate synthesis by Alcaligenes eutrophus, FEMS
Microbiol Lett, 57: 1-6.
He, W.N., Zhan, Z.M., Hu, P and Chen, G.Q., 1999. Microbial synthesis and characterization
of polyhydroxyalkanoates by strain DG17 from glucose, Acta Polymerica Sinica 6:709
- 714.

Health Protection Agency, 2008. Oxidase test, National Standard Method BSOPTP 26 Issue
x.http://www.hpa-standardmethods.org.uk/pdf_sops.asp.
Hendrickson, 1971, New sugar factory waste and their control 40th proceeding of the
T.S.S.C.T. October – November, PP 1552. Int. Res. Jr. Sodh, Samiksha and
Mulyankan 4, 307.
Henze, M., Harremoes, P., Jansen, J.C and Arvin, E., 2002. Wastewater Treatment-Biological
and chemical processes, 3rd edition, Springer-Verlag.
Herron, N.M, Anderson, M. and Dawes, E.A. 1978.A Survey of the Accumulation of Novel
Polyhydroxyalkanoates by Bacteria, Biotechnology Letters, 11(7): 471 - 476.
Hezayen, F.F., Gutierrez, M.C., Steinbuchel, A., Tindall, B.J and Rehm, B.H., 2010. Halopiger
aswanensis sp. nov., a polymer-producing, extremely halophilic archaeon isolated from
hypersaline soil, Int J Syst Evol Microbiol. 60:633-637.
Hezayen, F.F., Steinbuchel, A and Rehm, B. H., 2002. Biochemical and enzymological
properties of the polyhydroxybutyrate synthase from the extremely halophilic archaeon
strain 56, Arch Biochem Biophys. 403(2): 284–291.
Hii Ai Yieng., Habibollah Younesi., Aliakbar Zinatizadeh and Ghasem Najafpour., 2005.
Effect of organic loading on performance of rotating biological contactors using Palm
Oil Mill effluents, Process Biochemistry, 40 (8): 2879-2884.

Himi Husme Bin Abd Majid., 2007. Screening and Characterization of Pha-Producing
bacteria From Activated Sludge, Thesis submitted to the University Technology
Malaysia.

Hiraishi, A and Khan, S.T. 2003. Application of polyhydroxyalkanoates for denitrification in

water and wastewater treatment. Applied Microbiology and Biotechnology. 61: 103-109.
Hobson, P. N., Bousefield, S and Summers, R., 1981. Methane production from agricultural and
domestic wastes. Energy from wastes series, Applied Science Publishers, London.
Hobson, P.N and Stewart, C.S., 1970. Growth of two rumen bacteria in mixed culture. J. Gen.
Microbiol 63 (3): xi - xi.
Hobson, P.N and Summers, R., 1965. Continuous culture of some anaerobic and facultative
anaerobic rumen bacteria. J. Gen. Microbiol. 38 : 167 -180.
Hocking, P.J and Marchessault, R.H.1994. Chemistry and technology of Biodegradable
Polymers. In Griffin GJL (Eds). Blackie Academic, New York: 48-96.
Holmes, P. A., 1985. Applications of PHB - a microbially produced biodegradable thermoplastic.
Phys. Technol. 16: 32 - 36.
Holmes, P.A and Lim, G.B. March 1990. Separation process. US patent Application No.
4910145.
Holt, J., Krieg, N., Sneath, P., Stealey, J and Williams, S., 1994. Bergey’s Manual of
Determinative Bacteriology, 9th ed. Williams & Wilkins, Baltimore, MD.
Hori, K., Marsudi, S and Unno, H., 2002a. Simultaneous production of polyhydroxyalkanoates
and Rhamnolipids by Pseudomonas aeruginosa. Biotechnology and Bioengineering. 78
(6): 700-707.
Hormaeche, E and Edwards, P. R. 1958. Observations on the genus Aerobacter with a
description of two species, International Bulletin of Bacteriological Nomenclature and
Taxonomy 8: 111-115.
Hormaeche, E and Munilla, M. 1957. Biochemical tests for the differentiation of Klebsiella and
Cloacae, International Bulletin of Bacteriological Nomenclature and Taxonomy 7: 1–20.
Hormaeche, E and P. R. Edwards, 1960. A proposed genus Enterobacter, International Bulletin
of Bacteriological Nomenclature and Taxonomy 10(2), PP: 71 - 74.
Horowitz, D. M. and Sanders, J. K. M.1994. J. Am. Chem. Soc., 116: 2695 - 2702.
Houmiel, K.L., Slater, S., Broyles, D., Casagrande, L., Colburn, S., Gonzalez, K., Mitsky, T.A.,
Reiser, S.E., Shah, D., Taylor, N.B., Tran, M., Valentin, H.E and Gruys, K.J.1999.
Po1y(B-hydroxybutyrate) production in oilseed leukoplasts of Brassica napus. Planta
209: 547-50.
Hrabak, O., 1992. Industrial production of Poly-β-hydroxybutyrate. FEMS Microboiogy
Reviews. 103: 251-256.
Hu, W.F., Chua, H and Yu, P.H.F., 1997. Synthesis of poly (3-hydroxybutyrate-co3-
hydroxyvalerate) from activated sludge. Biotechnol. Lett., 19 (7): 695-698.
Huijberts, G. N. M., and Eggink, G., 1996. Production of poly (3-hydroxy- alkanoates by
Pseudomonas putida KT2442 in continuous cultures. Applied Microbiology and
Biotechnology. 46:233–239.
Huijberts, G.N., Eggink, G., de Waard P., Huisman, G.W and Witholt , B., 1992.
Pseudomonas putida KT2442 cultivated on glucose accumulates poly(3-
hydroxyalkanoates) consisting of saturated and unsaturated monomers. Appl Environ
Microbiol. 58:536–544.
Huisman, G.W., de Leeuw, O., Eggink, G and Witholt, B. 1989. Synthesis of poly (3-
hydroxyalkanoates) is a common feature of fluorescent pseudomonads. Appl. Environ.
Microbiol. 55 (8): 1949-1954.
Hungate, R. E., Dougherty, R. W., Bryant, M. P and Cello, R. M., 1952. Microbial and
physiological changes associated with acute digestion in sheep. Can.Vet.J. 42: 423–449.

Hustede, E., Steinbuchel, A and Schegal, H.G., 1993. Relationship between the
photoproduction of hydrogen and the accumulation of PHB in non-sulphur purple
bacteria, Applied Microbiology and Biotechnology. 39(1): 87 - 93.
Iggleden, G.J., 1981. Rotating biological contactors, Chemistry and Industry. 458: 1- 70.

Ilona Gasser., Henry Muller and Gabriele Berg., 2009. Ecology and characterization of
polyhydroxyalkanoate-producing microorganisms on and in plants. FEMS
Microbiology Ecology. 70 (1): 142-150.
In Jae Park, Jerng Chang Yoon, Seong Joo Park, Eung Ho Kim, Yeon-Jae Cho, and Kwang-
Soo Shin, 2003. Characterization of the Proteolytic Activity of Bacteria Isolated from a
Rotating Biological Contactor, The Journal of Microbiology, 41(2):73-77.
Indian Standards Institute (ISI), 1977.Tolerance Limit for Industrial Effluents Discharged on
Land for Irrigation Purposes, IS: 3307.
Itzigsohn, R. O., Yarden and Okon ,Y.,1995. Polyhydroxyalkanoate analysis in Azospirillum
brasilense. Can. J. Microbiol. 41(1): 73 - 76.
James, B.W., Mauchline, W.S., Dennis, P.J., Keevil, C.W and Wait, R., 1999. Poly-3
hydroxybutyrate in Legionella pneumophila, an energy source for survival in low-
nutrient environments. Appl. Environ. Microbiol. 65: 822-827.
Jeffrey B. Kaplan., Chandran Ragunath., Narayanan Ramasubbu and Daniel H. Fine., 2003.
Detachment of Actinobacillus actinomycetemcomitans biofilm cells by an endogenous
beta-hexosaminidase activity. J. Bacteriol. 185(16): 4693–4698.
Jendrossek, A., Selchow, O and Hoppert, M., 2007. Poly(3-hydroxybutyrate) granules at the
early stages of formation are localized close to the cytoplasmic membrane in
Caryophanon latum Appl. Environ. Microbiol., 73: 586 - 593.
Jendrossek, D. and Handrick, R., 2002. Microbial degradation of polyhydroxyalkanoates.
Annu Rev Microbiol 56: 403–432.
Jendrossek, D., 1998. Microbial degradation of polyesters: a review on extracellular poly-
(hydroxyalkanoic acid) depolymerase. Polym Degrad Stab.59:317–25.
Jensen, P. R., Dwight , R and Fenical, W., 1991. Distribution of actinomycetes in near-shore
tropical marine sediments. Appl. Environ. Microbiol. 57(4):1102-1108.
Jensen, T.E and Sicko, L.M., 1971. Fine structure of poly-ß-hydroxybutyric acid granules in a
blue-green alga, Chlorogloea fritschii. J. Bacteriol. 106 (2):683–686
Jiang, Y., Song, X., Gong, L., Li, P., Dai, C and Shao, W., 2008. High poly (3 –
hydroxybutyrate) production by Pseudomonas fluorescens A2a5 from inexpensive
substrates. Enzy. Microbial. Technol., 42 (2): 167-172.
Johannes Rousk, 2009, On the Ecology of Saprotrophic Fungi and Bacteria in Soil: Biotic and
Abiotic Control of Growth Rates, Applied and Environmental Microbiology.75 (6):
1589–1596.
John, M.E and Keller, G., 1996. Metabolic pathway engineering in cotton: biosynthesis of
polyhydroxybutyrate in fiber cells, Proc. Natl. Acad. Sci. USA. 93: 12768 - 12773.

Johnson, K., van Geest, J., Kleerebezem, R and van Loosdrecht, M.C.M., 2010. Short- and long-
term temperature effects on aerobic polyhydroxybutyrate producing mixed cultures.
Water Res.44:1689–1700.
Johnson, N., Revenga, C and Eche Veria, J., 2001. Managing water for People and Nature.
Science. 292(5519): 1071 – 1072.
Johnstone, B.,1990. A throw away answer. Far Eastern Econ. Rev. 147(6): 62-63.
Jordan, E. O. 1890. A report on certain species of bacteria observed in sewage, Reports of the
Massachusetts Board of Public Health, 821- 844.
Juretschko, S., Loy, A., Lehner, A and Wagner, M. 2002. The microbial community composition
of a nitrifying- denitrifying activated sludge from an industrial sewage treatment plant
analyzed by the fullcycle rRNA approach. Systematic and Applied Microbiology 25 (1):
84 - 89.
Kadouri, D., Jurkevitch, E., Okon, Y and Castro, S., 2005. Ecological and agricultural
significance of bacterial polyhydroxyalkanoates. Crit. Rev. Microbiol. 31 (2), 55-67.
Kalia, V.C and Joshi, A.P., 1995. Conversion of waste biomass (pea-shells) into Hydrogen
methane through anaerobic digestion, Bioresource technology 53: 165 - 168.
Kalia, V.C., Kumar, A., Jain, S.R and Joshi, A.P., 1992. Biomethonation of plant Materials,
Bioresource technology.41 (3):209-212.
Kalia, V.C., Raizada, N and Sonakya, V., 2000. Bioplastics, Journal of scientific and Industrial
research. 59 (6): 433-445.
Kannan, N., Rajasekaran, . and Vallinayagam, P. 1991. "Multivariate correlation analysis of
water quality parameters of industrial effluents". Indian J. Environ. Protect., (II)ll: 857-
860.
Kanokphorn Sangkharak and Poonsuk Prasertsan., 2012. Screening and identification of
polyhydroxyalkanoates producing bacteria and biochemical characterization of their
possible application, The Journal of General and Applied Microbiology
58 (3):173-182.
Kaplan, J.B., Ragunath, C., Ramasubbu, N and Fine, D.H., 2003. Detachment of Actinobacillus
actinomycetemcomitans biofilm cells by an endogenous beta-hexosaminidase activity.
Journal of Bacteriology 185 (16): 4693–8.
Kapoor, A., Kuiper, A., Bedard, P and Gould, W.D. 2003. “Use of a rotating biological
contactor for removal of ammonium from mining effluents”, The European Journal of
Mineral Processing and Environmental Protection (3) PP: 88.
Kauffmann, F., 1954. Enterobacteriaceae. 2nd ed. Copenhagen, Munksgaard.
Kawai, F. 1995. Breakdown of plastics and polymers by microorganisms. Advances in
Biochemical Engineering / Biotechnology. 52:152-193.
Kek, Y.K., Chang, C.W., Amirul, A.A and Sudesh, K., 2010. Heterologous expression of
Cupriavidus sp. USMAA2-4 PHA synthase gene in PHB−4 mutant for the production of
poly (3- hydroxybutyrate) and its copolymers, World J. Microbiol. Biotechnol., 26(9):
1595-1603.
Kemnitzer, J.E., McCarthy, S.P and Gross, R.A. 1993. Syndiospecific Ring-Opening
Polymerization of B-Butyrolactone To Form Predominantly Syndiotactic Poly(B-
hydroxybutyrate) Using Tin(IV) Catalysts. Macromolecules 26: 6143-6150.

Kessler, B and Palleroni, N.J. 2000. Taxonomic implications of synthesis of poly-3-

hydroxybutyrate and other polyhydroxyalkanoates by aerobic pseudomonas. Int J Sys
Evol Microbiol. 50:711-713.

Khanna, S and Srivastava, A.K., 2004. Recent advances in microbial polyhydroxyalkanoates.

Process Biochemistry. 40(2): 607-619.
Khardenavis, A.A., Kumar, M.S., Mudliar, S.N and Chakrabarti, T., 2007. Biotechnological
conversion of agro-industrial wastewaters into biodegradable plastic, poly β-
hydroxybutyrate. Bioresource Technology, 98(18):3579-3584.
Khardenavis, A.A., Sureshkumar, M and Chakrabarti, T., 2003. Biodegradable plastics
production from industrial waste water by activated sludge, 44th Annual Conference of
Association of Microbiologists of India: 107.

Khatipov, E., Miyakea, M., Miyakec, J and Asadaa, Y., 1998. Accumulation of poly-β
hydroxybutyrate by Rhodobacter sphaeroides on various carbon and nitrogen
substrates, FEMS Microbiology Letters. 162(1): 39 - 45.

Kim, B. S and Chang, H. N., 1995. Control of glucose feeding using exit gas data and its
application to the production of PHB from tapioca hydrolysate by Alcaligenes
eutrophus. Biotechnol. Techniques. 9: 311-314.
Kim, B.S, Lee, S.C., Lee, S.Y., Chang, H.N., Chang, Y.K and Woo, S.I., 1994. Production of
poly (3-hydroxybutyric acid) by Ralstonia eutropha with glucose concentration control.
Biotechnology and Bioengineering, 43: 892 -898.
Kim, B.S., 2000. Production of poly (3-hydroxybutyrate) from inexpensive substrates. Enzyme
and Microbial Technology. 27 (10): 774-777.
Kim, G.J., Lee, I.Y., Yoon, S.C., Shin, Y.C and Park, Y.H.,1997. Enhanced yield and a high
production of medium-chain-length poly (3-hydroxyalkanoates) in a twostep fed-batch
cultivation of Pseudomonas putida by combined use of glucose and octanoate. Enzyme
Microbial Technology, 20: 500.
Kim, M., Baek, J and LEE, J.K., 2006. Comparison of H2 accumulation by Rhodobacter
sphaeroides KD131 and its uptake hydrogenase and PHB synthase deficient mutant.
International Journal of Hydrogen Energy. 31(1):121-127.
Kim, W., 2000. Production of poly (3-hydroxybutyrate) from inexpensive substrates. Enzyme
and microbial technology 27: 774 - 777.
Kim, W., Kim, P., Lee and Kim, J.H., 1996. High production of poly-b-hydroxybutyrate
(PHB) from Methylobacterium organophilum under potassium limitation. Biotechnol.
Lett. 18: 25 - 30.
Kim, Y. B., Lenz, R. W and Fuller, R. C., 1991. Preparation and characterization of poly (ß-
hydroxyalkanoates) obtained from Pseudomonas oleovorans grown with mixtures of 5-
phenylvaleric acid and n-alkanoic acids. Macromolecules. 24: 5256- 5260.
Kim, Y.B and Lenz, R.W. 2001. Polyesters from microorganisms, Adv. Biochem. Eng,
Biotechnology. 71: 51 -79.
Kirschbaum, M. U. F.,2000. Will changes in soil organic carbon act as a positive or negative
feedback on global warming? Biogeochemistry. 48:21- 51.
Kirschbaum, M.U.F., 1995. The temperature dependence of soil organic matter decomposition,
and the effect on global warming, Soil Biology & Biochemistry 27: 753 -760.
Klemz, T.2002. Paper mak distrib. I:16.
Klinke, S., de Roo, G., Witholt, B., and Kessler, B., 2000. Role of phaD in accumulation of
medium-chain-length poly (3-hydroxyalkanoates) in Pseudomonas oleovorans. Appl.
Environ. Microbiol. 66 (9): 3705–3710.
Kolattukudy, P.E. 2001. Polyesters in higher plants.Biopolyesters. Advances in Biochemical
Engineering Biotechnology. 71: 1-49.
Koller, M., Bona, R., Braunegg, G., Hermann, C., Horvat, P and Kroutil, M., 2005. Production of
polyhydroxyalkanoates from agricultural waste and surplus materials.
Biomacromolecules. 6 (2): 561-565.
Koller, M., Bona, R., Chiellini, E., Fernandes, E.G., Horvat, P., Kutschera, C., Hesse, P and
Braunegg, G., 2008. Polyhydroxyalkanoate production from whey by Pseudomonas
hydrogenovora. Bioresource Technol. 99: 4854-4863.
Koller, M., Hesse, P., Salerno, A., Reiterer, A and Braunegg, G., 2011. A viable antibiotic
strategy against microbial contamination in biotechnological production of
polyhydroxyalkanoates from surplus whey. Biomass Bioenerg. 35(1):748 – 753.
Kong, Y.H., Beer, M., Seviour, R.J., Lindrea, K.C., Rees, G.N. 2001. Structure and functional
analysis of the microbial community in an aerobic: anaerobic sequencing batch reactor
(SBR) with no phosphorus removal. Syst Appl Microbiol. 24(4):597-609.
Kornegay, B. H., 1972. Modeling and Simulation of Fixed Film Biological Reactors, Georgia
Institute of Technology.
Kornegay, B.H and Andrews, J.F., 1968. Kinetics of fixed film biological reactors. J. Water
Pollut. Control Fed. 40 (11): R460–R468.
Kousar Yasmeen; Muhammad Ali Versiani; Rafee Arain; Qamarul Haque; Nasiruddin Khan;
Syed Asghar Ali and Aashiq Ali Langha, 2010.” Enhanced Metal Levels in Vegetables
and Farm Soil irrigated with Industrial Waste Water”, J. Appl. Sci. Environ. Manage.
Koutinas, A.A., Xu, Y., Wang, R and Webb, C., 2007. Polyhydroxybutyrate production from a
novel feedstock derived from a wheat-based biorefinery. Enzyme and Microbial
Technology. 40(5): 1035- 1044.
Kovarova, K., Zehnder, A. J and Egli, T., 1996. Temperature-dependent growth kinetics of
Escherichia coli ML 30 in glucose-limited continuous culture, J. Bacteriol. 178(15):4530
- 4569.
Kragelund, C., Kong, Y., Van der Waarde, J., Thelen, K., Eikelboom, D., Tandoi, V., Thomsen,
T.R and Nielsen, P.H.2006. Ecophysiology of different filamentous Alphaproteobacteria
in industrial wastewater treatment plants. Microbiology 152(10): 3003 - 3012.
Kragelund, C., Levantesi Caterina, Borger Arjan, Thelen, K., Eikelboom, D., Tandoi, V., Kong,
Y., Van Der Waarde, J., Krooneman, Rossetti, Thomsen and Nielsen. 2007. Identity,
abundance and ecophysiology of filamentous Chloroflexi species present in activated
sludge treatment plants. FEMS Microbiology Ecology 59(3): 671 - 682.
Kris Pynaert, Barth F. Smets, Stijn Wyffels, Daan Beheydt, Steven D. Siciliano, and Willy
Verstraete , 2003. Characterization of an Autotrophic Nitrogen-Removing Biofilm from a
Highly Loaded Lab-Scale Rotating Biological Contactor, Applied And Environmental
Microbiology, 69(6):3626–3635.
Kristijanto, A.I., Handayani., Widhi., Anna Levi and Purwanti Asih., 2011.The Effectiveness of
Anaerobic Baffled Reactorand Rotating Biological Contactor in Batik Wastewater
Treatment. Makara Teknologi. 15(2):168- 172.
Kumar, S.K., Rammohan, V., Sahayam, J.D., Jeevanandam, M., 2009. Environ Monit Assess.,
159 (1):341–351.
Kundu, B. S and Gaur, A. C. 1984. Rice response to inoculant with N-fixing and P-
solubilizing microorganisms. Plant Soil, 79 (2): 227-234.
Kuno Jung., 1999. synthesis of functionalized Poly (3-hydroxyalkanoates) (PHAs), from
organic solvents by Pseudomonas oleovorans. PhD dissertation submitted to Swiss
Federal Institute of Technology.
Kusdiyantini, E., Gaudin, P., Goma, G and Blanc, P.J.1998. Growth kinetics and astaxanthin
production of Phaffia rhodozyma on glycerol as a carbon source during batch
fermentation. Biotechnology Letters. 20(10): 929-934.
Kushwah Ram Kumar, Malik Suman and Singh Archana,2012. “Water Quality Assessment of
Raw Sewage and Final Treated Water with Special Reference to Waste Water Treatment
Plant Bhopal, MP, India”, Research Journal of Recent Sciences, (1): 185.
Labella, S.A., 1972. Treatment of Winery Wastes by Aerated Lagoon, Activated Sludge and
Rotating Biological Contactor, Engr. Bulletin of Purdue University: 803.
Labuzek, S and Radecka, I., 2001. Biosynthesis of PHB tercopolymer by Bacillus cereus UW 85.
Journal of Applied Microbiology. 90(3): 353-357.
Lafferty, R. M., Korsatko, B and Korsatko, W., 1988. Microbial production of poly-hydroxy-
butyric acid. In: A comprehensive treatise in biotechnology (H.-J. REHM, Ed.) 6b: 135-
176.
Lageveen, R. G., Huisman, G. W., Preusting, H., Ketelaar, P., Eggink, G and Witholt, B., 1988.
Formation of polyesters by Pseudomonas oleovorans: effect of Substrates on formation
and composition of poly-(R)-3-hydroxyalkanoates and poly-(R)-3-hydroxyalkenoates.
Appl. Environ. Microbiol. 54 (12): 2924-2932.
Lakkana Laopaiboon, Niphaphat Phukoetphim and Pattana Laopaiboon,2006. Effect of
glutaraldehyde biocide on laboratory-scale rotating biological contactors and biocide
efficacy, Electronic Journal of Biotechnology, (9), 2006.
Lakshmar, K., Rastogi N.K and Shamala, T.R., 2004. Simultaneous and comparative assessment
of parent and mutant strain of Rhizobium meliloti for nutrient limitation and enhance
polyhydroxyalkanoate (PHA) production using optimization studies. Process
Biochemistry. 39(12): 1977-1983.
Lampman, G.G., Caraco, N.F and Cole, J.J., 1999. Spatial and temporal patterns of nutrient
concentration and export in the tidal Hudson River. Estuaries 22: 285-96.
Langmuir, D. 1997, Aqueous Environmental Geochemistry, 600 pp., Prentice-Hall, Upper Saddle
River, N. J.
Le Digabel, F and Averous L., 2006. Effects of lignin content on the properties of lignocellulose-
based biocomposites. Carbohydr. Polym. 66: 537-545.
Leaf, T.A., Peterson, M.S., Stoup, S.K., Somers, D and Srienc, F., 1996. Saccharomyces
cerevisiae expressing bacterial polyhydroxybutyrate synthase produces poly-3-
hydroxybutyrate. Microbiology 142: 1169 - 1180.
Lear, G and Lewis, G.D. 2012. Microbial Biofilms: Current Research and Applications,
Caister Academic Press, ISBN 978-1-904455-96-7: 61-71.

Leaversuch, R., 2002. Biodegradable polyesters; Packaging goes green. Plastics Technology.
48(9): 66-73.
Lee, E.Y., Jendrossek, D., Schirmer, A., Choi, C.Y and Steinbuchel, A., 1995. Biosynthesis of
copolyesters consisting of 3-hydroxybutyric acid and medium –chain-length 3-
hydroxyalkanoic acids from 1, 3-butanediol or from 3-hydroxybutyrate by Pseudomonas
sp. A33. Applied Microbiology and Biotechnology. 42(6):901-909.
Lee, S and Yu, J., 1997. Production of biodegradable thermoplastics from municipal sludge
by two-stage bioprocess, Resources Conservations and recycling. 19(3): 151-164.
Lee, S. Y and Chang, H. N., 1995. “Production of Poly (3-hydroxybutyric Acid) by Recombinant
Escherichia coli Strains: Genetic and Fermentation Studies”, Can. J. Microbiol., 41(1):
207-215.
Lee, S.Y and Chang, H.N. 1995. Production of poly(hydroxyalkanoicacid), Adv. Biochem.
Engineering. Biotechnology. 52, 27-58.
Lee, S.Y., 1996. Bacterial polyhydroxyalkanoates, Biotechnology and bioengineering. 49 (1): 1-
14.
Lee, S.Y., Choi, J and Lee, S.H. 2000. Production of polyhydroxyalkanoates by fermentation of
bacteria. Macromolecular Symposia. 159(1): 259- 266.
Lee, S.Y., Choi, J and Lee, S.H., 2000. Production of polyhydroxyalkanoates by fermentation of
bacteria. Macromolecular Symposia. 159(1): 259- 266.
Lee, S.Y.1996. Bacterial polyhydroxyalkanoates. Biotechnology and Bioengineering. 49 (1): 1-4.
Lefebvre, G., Rocher, M and Braunegg, G., 1997. Effects of Low Dissolved-Oxygen
Concentrations on Poly-(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Production by
Alcaligenes eutrophus, Applied and Environmental Microbiology 63 (3): 827 – 833.

Lemoigne, M., 1926. Produits de doshydration et de polymorization de acid oxybutyrique,

Bull. Soc. Chim. Biol., 8: 770 –782.
Levy, G.M. 1993. Packaging in the environment. Published by Blackie Academic and
professional.
Lewandowsiky, Z and Beyenal, H. 2003. Mass transport in heterogeneous biofilms. In: Biofilms
in Wastewater Treatment, An interdisciplinary Approach, Bishop, Wuertz and Wilderer
(Ed), IWA publishing House, UK: 145 - 176.
Liangqi, Z., Jingfan, X., Tao, F and Haibin, W., 2006. Synthesis of poly (3 hydroxybutyrate co-
3- hydroxyoctanoate) by a Sinorhizobium fredii strain. Letters in Applied Microbiology.
4:344-349.
Libey, G. S., 1993. Evaluation of a drum filter for removal of solids, Techniques for modern
aquaculture. American Society of Agricultural Engineers, St. Joseph, Michigan, J.K.
Wang, editor.
Liebergesell, M., Schmidt, B., and Steinbuchel, A.,1992. Isolation and identification of granule-
associated proteins relevant for poly(3-Hydroxyalkanoic Acid) biosynthesis in
chromatium-vinosum D. FEMS Microbiol. Lett. 99: 227–232.
Lillo, J. G and Rodriguer-Valera, F., 1990. Effects of culture conditions on poly (β-
hydroxybutyric acid) production by Haloferax mediterranei. Appl. Environ. Microbiol.
56 (8): 2517–2521.
Linko, S and Vaheri, H. J. S., 1993. Production poly-beta-hydroxybutyrate on lactic acid by
Alcaligenes eutrophus H16 in a 3-l bioreactor. Enzyme Microb Technol. 15(5): 401– 406.
Liu, F., Li, W., Ridgway, D and Gu, T., 1998. Production of poly-β-hydroxybutyrate on
molasses by recombinant Escherichia coli. Biotecnology Letters. 20 (4): 345- 348.
Loosdrecht, M.C.M., Benthum, W.A.J and Heijnen, J.J. 2000. Integration of nitrification and
denitrification in biofilm airlift suspension reactors. Wat. Sci. Tech. 41(4-5): 97-103.
Lopez, N.I., Floccari, M.E., Steinbuchel, A., Garcia, A.F and Mendez, B.S., 1995. Effect of
po1y (3 -hydroxybutyrate) content on the starvation-survival of bacteria in natural
waters, FEMS Microbiol. Ecol. 16: 95.
Lopez, N.I., Ruiz, J.A and Mendez, B.S.,1998, Survival of poly-3-hydroxybutyrateproducing
bacteria in soil microcosms. World J. Microbiol. Biotechnol. 14: 681-684.
Lorcks, J 1998. Properties and applications of compostable starch-based plastic material.
Polymer Degradation and Stability. 59 (1–3): 245-249.

Lorrungruang, C., Martthong, J., Sasaki, K and Noparatnaraporn, N., 2006. Selection of
photosynthetic bacterium Rhodobacter sphaeroides 14F for polyhydroxyalkanoate
 production with two-stage aerobic dark cultivation. Journal of Bioscience and
Bioengineering.101(2): 128 - 131.

Louis Paul-Anthony Paladino., 2009. Screening, optimization and extraction of

polyhydroxyalkanoates and peptidoglycan from Bacillus megaterium, Dissertations,
master's theses and master's reports, Michigan Tech.

Luengo, J.M., Garcia, B., Sandoval, A., Naharro, G., and Oliver, E.R., 2003. Bioplastics from
microorganisms, Current Opinion in Biotechnology. 6: 251 - 260.

Luzier, W.D. 1992. Materials derived from biomass/biodegradable materials. Proc. Natl.Acad.
Sci. U S A. 89: 839-842.
Mackie, R. I. and Gilchrist, F. M. C., 1978. Microbiological and chemical changes in the rumen
during the stepwise adaptation of sheep to high concentrate diets, J. Agric. Sci. Camb. 90:
241 - 254.
Macrae, R.M and Wilkinson, J.F., 1958. Poly-beta-hyroxybutyrate metabolism in washed
suspensions of Bacillus cereus and Bacillus megaterium. Journal of General
Microbiology. 19:210-222.
Madigan, M. T and Martinko, J. M. 2005. Brock Biology of Microorganisms, 11th ed.,
Prentice Hall. Upper Saddle River, New Jersey, USA.
Madigan, M., Martinko, J and Parker, J., 2003. Brock Biology of Microorganisms, 10th ed.
Upper Saddle River: Pearson Education.
Madison, L and Huisman, G. W., 1999. Metabolic engineering of poly (3- hydroxyalkanoates):
from DNA to plastic. Microbiol. Mol. Biol. Rev. 63 (1): 21–53.
Magda M. Aly., Mona O. Albureikan., Haddad El Rabey 2,4 and Saleh A. Kabli1., 2013. Effects
of culture conditions on growth and poly- β-hydroxybutyric acid production by Bacillus
cereus MM7 isolated from soil samples from Saudi Arabia. Life Science Journal.
10(4):1884 – 1891.
Mahananda, M. R., Mohanty, B. P. and Behera Mahananda, N. R. 2010, “Physico-Chemical
analysis of surface water and ground water of Bargarh District, Orissa, India,”
International Journal of Research and Review in Applied Sciences 2: 26 -30.
Mahishi, L.H., Tripathi, G and Rawal, S.K., 2003. Poly(3-hydroxybutyrate) (PHB) synthesis by
recombinant Escherichia coli harbouring Streptomyces aureofaciens PHB biosynthesis
 genes: Effect of various carbon and nitrogen sources Microbiological Research.158
(1):19 -27.
Majid, H. U. B. A., 2008. Screening and characterization of PHA producing Bacteria from
activated sludge.
Majid, M.I.A., Akmal, D.H., Few, L.L., Agustien, A., Toh, M.S., Samian, M.R., Najimudin, N
and Azizan, M.N., 1999. Production of poly(3-hydroxybutyrate) and its copolymer
poly(3-hydroxybutyrate-co-3- hydroxyvalerate) by Erwinia sp. USMI-20. Int J Biol
Macromol 25 (1 – 3):95–104.
Malandra, L., Wolfaardt, G., Zietsman, A and Viljoen, M., 2003. Bloom, Microbiology of a
biological contactor for winery waste water treatment, Water Res. 37 (17): 4125 - 4134.
Mallin, M.A., 2000. Impacts of industrial-scale swine and poultry production on rivers and
estuaries. American Scientist 88: 26-37.
Mallin, M.A., Williams, K.E., Esham, E.C and Lowe, R.P., 2000. Effect of human development
on bacteriological water quality in coastal watersheds. Ecological Applications 10:1047-
1056.
Mara, D.D., 2010. CIWEM Good Practise in Water and Environmental Management: Natural
Wastewater Treatment. Aqua Enviro Technology Transfer, Wakefield, UK.
Marangoni, C., Furigo A Jr and de Aragao, G.M.F., 2002. Production of poly (3-
hydroxybutyrate-co-3-hydroxyvalerate) by Ralstonia eutropha in whey and inverted sugar
with propionic acid feeding. Proc Biochem. 38:137–141.
Mareschal, F., 2003. Biodegradable Plastics Views of APME (Association of Plastics
Manufacturers in Europe) in E. Chiellini, R. Solaro eds. Biodegradable Polymers and
Plastics, Kluwer Academic/Plenum Publishers NY: 67-71.
Marie Pettersson., 2004. Factors Affecting Rates of Change in Soil Bacterial Communities,
Doctoral thesis, Lund University, Sweden.

Martin, D.P and Williams, S.F., 2003. Medical applications of poly-4-hydroxybutyrate: a

strong flexible absorbable biomaterial. Biochem Eng J. 16:97-105
Martinez Toledo, M.V., Gonzalez Lopez, J., Rodelas, B., Pozo, C and Salmeron, V., 1995.
Production of poly-beta-hydroxybutyrate by Azotobacter chroococcum H23 in
chemically defined medium and alpechin medium, J appl bacterial, Oxford, New York,
Blackwell Scientific. 78(4): 413 - 418.
Massadeh, A.M., Al-momani, F.A and Haddad, H.I., 2005. Removal of lead and cadmium by
halophilic bacteria isolated from the Dead Sea shore, Jordan, Trace Element Research,
108: 259-269.

Mathieu, F. Jr., and Timmons, M.B, 1993.Time dependent BOD aquacultural wastes in water
recirculating rainbow trout systems, Techniques for modern aquaculture. American
Society of Agricultural Engineers, St. Joseph, Michigan, J.K. Wang, editor.
Matinez-Toledo, V., Gonzalez-Lopez, J., Rodelas, B., Pozo, C and Salmeron, V., 1995.
Production of poly-beta-hydroxybutyrate by azotobacter chroococcum H23 in
chemically defined medium and alpechin medium. J. appl. Bact.78: 413 -418.
Matsui, T., Sato, H., Yamamuro, H., Shinzato, N., Matsuda, H., Misawa, S and Sato, S., 2008.
High cell density cultivation of recombinant E. coli for hirudin variant 1 production by
temperature shift controlled by pUC18-based replicative origin, Appl. Microbiol.
Biotechnol. 80:779-783.
Mayer, F and Hoppert, M., 1997. Determination of the thickness of the boundary layer
surrounding bacterial PHA inclusion bodies, and implications for models describing
the molecular architecture of this layer, Journal of Basic Microbiol., 37(1): 45 - 52.
Mayer, F., Madkour, M.H., Pieper-Furst, U., Wieczorek, R., Liebergesell, M and Steinbuchel,
A. 1996. Electron microscopic observations on the macromolecular organization of
the boundary layer of bacterial PHA inclusion bodies. J. Gen. Appl. Microbiology. 42:
445 - 455.
Mba, D., Bannister, R.H and Findlay, G.E., 1999. Mechanical redesign of the rotating biological
contactor. Water Research 33 (18): 3679 – 3688.
Mc Carty, P. L and Williamson, K., 1976. A Model of Substrate Utilization by Bacterial
Films, Journal of Water Pollution Control. Fed.
Meenakshi, Garg, V.K., Kavita, Renuka, and Anju Malik, 2004. Groundwater quality in some
villages of Haryana, India: focus on fluoride and fluorosis, Jour. Hazard. Mater.,
106B, PP: 85.
Melamane, X.L, Strong, P.J. and Burgess, J.E., 2007. “Treatment of Wine Distillery
Wastewater: A Review with Emphasis on Anaerobic Membrane Reactors”, S. Afr. J.
Enol. Vitic., (28), PP 25.
Mergaert, J., Anderson, C., Wouters, A., Swings, J and Kersters, K., 1992. Biodegradation of
polyhydroxyalkanoates. FEMS Microbiol Rev. 9: 317–321.
Merrick, J.M and Doudoroff, M., 1964. Depolymerization of poly-β -hydroxybutyrate by an
intracellular enzyme system. J. Bacteriol. 88: 60-71.
Merrick, J.M., Steger, R and Dombroski, D., 1999. Hydrolysis of native poly (hydroxybutyrate)
granules (PHB), crystalline PHB, and artificial amorphous PHB granules by intracellular
and extracellular depolymerases. Int. J. Biol. Macromol. 25: 129-134.
Metcalf and Eddy. 2003. Wastewater Engineering. Treatment and Reuse. Tchobanoglous, G.,
Burton, F.L., Stensel, H.D (Eds). 4th Ed. McGraw Hill, Inc., USA
Michael Dunne, Jr., 2002. Bacterial Adhesion: Seen Any Good Biofilms Lately? Clin.
Microbiol. Rev.
Michael Knoll., Thomas M Hamm., Florian Wagner., Virginia Martinez and Jurgen Pleiss.,
2009. The PHA Depolymerase Engineering Database: A systematic analysis tool for
the diverse family of polyhydroxyalkanoate (PHA) depolymerases, BMC
Bioinformatics 10:89.
Mignard, S and Flandrois, J.P. 2006. 16S rRNA sequencing in routine bacterial identification: a
30-month experiment, J Microbiol. Methods 67(3):574-81.
Minoru, A., Yasuo, T and Yoshiharu, D., 1992. Production of poly (3-hydroxyalkanoates) by
a bacterium of the genus alcaligenes utilizing long-chain fatty acids. Appl. Microbiol.
Biotechnol. 37: 689-701.
Mitchell, P., 1961. Coupling of phosphorylation to electron and hydrogen transfer by a
chemiosmotic type of mechanism, Nature (London) 191: 144 - 148.
Mittal, S.K. and Verma, N.1970. Indian J. Env. Prot., 17(6): 426-429
Mobarry, B.K., Wagner, M., Urbain, V., Rittmann, B.E., Stahl, D.A. 1996. Phylogenetic probes
for analyzing abundance and spatial organization of nitrifying bacteria. Applied and
Environmental Microbiology 62, PP: 2156.

Mohd. Fadil Md. Din., Zaini Ujang., Van Loosdrecht, M. C. M and Mohd. Azlan Ahmad., 2008.
Polyhydroalkanoates (PHAs) Production from Saponified Sunflower Oil in Mixed
Cultures under Aerobic Condition, Jurnal Teknologi, 48 (F): 1- 19.
Moller, V.1955. Simplified tests for some aminoacid decarboxylases and for the arginine
dihydrolase system. Acta Pathologica Microbiologica Scandinavica 36(2): 158 - 172.
Momba, M.N.B., Cloete, T., Venter, S. and Kfir, R. 2000 Influence of disinfection processes on
the microbial quality of potable groundwater in a laboratory-scale system model. Journal
of Water Supply: Research and Technology – Aqua 49(1): 23–33.
Moore, S.2003. Bans on bags, other plastic disposables expand in Asia: environmental
legislations widens its grip. Modern plastic International. 33(2):58.

Morgesin, R and Schinner, F., 2001. Potential of halotolerant and halophilic microorganisms
for biotechnology, Extremophiles. 5(2):73 -83.

Morinaga, Y., Yamanaka, S., Ishizaki, A and Hirose, Y., 1978. Growth characteristics and cell
composition of Alcaligenes eutrophus in chemostat culture. Agr. Biol. Chem. 42: 439–
444.
Morse, G. K, Lester, J. N and Perry, R. 1993. The economic and environmental impact of
phosphorus removal from wastewater in the European Community. Selpher
Publications, London.

Mukheibir, P and Sparks, D., 2003. Water resources management and climate change in South
Africa: Visions, driving factors and sustainable development indicators. Report for Phase
I of the Sustainable Development and Climate Change project. Energy and Development
Research Centre. University of Cape Town.
Muller, S and Babel, W. 2003. Analysis of bacterial DNA patterns-An approach for controlling
biotechnological processes. J Microbiol Methods: 55 (3): 851-8.
Muserref Turkmen, Edward F. Walther, Scott Andres, Anastasia Chirnside, William Ritter,
2008. Evaluation of Rapid Infiltration Basin Systems (Ribs) For Wastewater Disposal,
Submitted to: Delaware Department of Natural Resources and Environmental Control.
Nagarajan, S., Swaminathan, M. and Sabarathinam P. L, 1993. Poll. Res., 12(4): 245 – 250.
Nahid, P., Vossoughi, M and Alemzadeh, I., 2001. Treatment of bakers yeast with a Biopack
system. Process Biochem. 37: 447-451.
Nair, I.C., Pradeep, S., Ajayan, M.S., Jayachandran, K and Shashidhar, S., 2009. Accumulation
of intracellular polyhydroxybutyrate in Alcaligenes sp. d(2) under phenol stress.
Appl.Biochem. Biotechnol. 159: 545-552.
Nakashita, H., Arai, Y., Yoshioka, K., Fukui, T., Doi, Y and Usami, R.1999. Production of
biodegradable polyester by a transgenic tobacco, Biosci. Biotechnol. Biochem. 63: 870
- 874.
Narancic, T., Kenny, S.T., Djoki, C L., Vasiljevic, B., O'Connor, K.E and Nikodinovic-Runic,
J., 2012. Medium-chain-length polyhydroxyalkanoate production by newly isolated
Pseudomonas sp. TN301 from a wide range of polyaromatic and monoaromatic
hydrocarbons, Journal of Applied Microbiology.
Nataraj, S.K., Hosamani, K.M and Aminabhavi, T.M., 2006. Distillery waste water treatment by
the membrane – based nanofiltration and reverse osmosis process. Water Research 40
(12): 2349–2356.
Nath, A., Dixit, M., Bandiya, A., Chavda, S and Desai, A.J., 2008. Enhanced PHB production
and scale up studies using cheese whey in fed batch culture of Methylobacterium sp.
ZP24. Bioresource Technol., 99(13): 5749-5755.
Neytzell-De Wildes,1991. Reassessment of the Strategy with respect to industrial effluent
discharge with special reference to advanced technology treatment methods: phase I.
Industrial effluent discharge problem areas. WRC Report No. 407/1/92.
Nicolella, C., Van Loosdrecht, M.C.M and Heijnen, J.J., 2000. Waste water treatment with
particulate biofilm reactors, J.Biotechnol.80: 1.
Nielsen, P.H., Mielczarek, A.T., Kragelund, C., Nielsen, J.L., Saunders, A.M., Kong, Y.,
Hansen, A.A and Vollertsen, J. 2010. A conceptual ecosystem model of microbial
communities in enhanced biological phosphorus removal plants. Water Research 44(17):
5070- 5088.
Nighat Naheed., Nazia Jamil and Shahida Hasnain., 2011, Screening of contaminated soils for
biodegradable plastic producing bacteria and profiling of their resistance markers,
African Journal of Microbiology Research. 5(24):4097-4104.
Nikel, P.I., Pettinari, M.J., Mendez, B.S and Galvagno, M.A., 2005. Statistical optimization of a
culture medium for biomass and poly(3- hydroxybutyrate) production by a recombinant
Escherichia coli strain using agroindustrial byproducts. Int Microbiol. 8:243–250.
Nishida, H and Tokiwa, Y., 1993. Distribution of poly(β-hydroxybutyrate) and poly (ε-
caprolactone) aerobic degrading microorganisms in different environments. J Environ
Polym Degrad .1:227–33.
Nur Ceyhan and Guven Ozdemir., 2011. Poly-β-hydroxybutyrate (PHB) production from
domestic wastewater using Enterobacter aerogenes 12Bi strain, Afr. J. Microbiol. Res.
5(6): 690-702.
Odum, E. P. 1969. In: Fungal Physiology, 2nd Edition. Saunders, Philadelphia, Shannon
Armstrong, Benny Freeman, Anne Hiltner and Eric Baer. (2012)Gas permeability of
melt-processed poly (ether block amide) copolymers and the effects of orientation,
53:1383 -1392.
Oeding, V and Schlegel, H.G., 1973. P-Ketothiolase from Hydrogenomonas eutropha H16 and
its significance in the regulation of poly-B-hydroxybutyrate metabolism. Biochem. J. 134
(1): 239-248.
Oehmen, A., Teresa Vives, M., Lu, H., Yuan, Z and Keller, J. 2005. The effect of pH on the
competition between polyphosphate-accumulating organisms and glycogen-accumulating
organisms. Water Research 39(15): 3727 - 3737.
Ojumu, T. V., Yu, J and Solomon, B. O., 2004. Production of Polyhydroxyalkanoates, a
bacterial biodegradable polymer, African Journal of Biotechnology. 3 (1): 18 - 24.

Okon, Y and R, Itzigsohn., 1992. Poly-β-hydroxybutyrate metabolism in Azospirillum brasilense

and the ecological role of PHB in the rhizosphere. FEMS Microbiol. Rev. 103: 131 – 140.
Olinda F. Canhoto and Naresh Magan, 2002, 2003. Potential for Detection of Microorganisms
and Heavy Metals in Potable Water using Electronic Nose Technology, Biosensors and
Bioelectronics, Selected papers from the Seventh World Congress on Biosensors Kyoto,
Japan 18 (5 – 6):751-754.
Oliveira, F.C., Freire, D.M.G and Castilho, L. R., 2004. Production of poly (3- Hydroxybutyrate)
by solid-state fermentation with Ralstonia eutropha, Bioresource technology. 25: 65 - 70.
Oliveira, R., Azeredo, J and Teixeira, P., 2003. The importance of physicochemical properties in
biofilm formation and activity. In: Wuertz S, Bishop PL, Wilderer PA, editors. Biofilms
in Wastewater Treatment: An Interdisciplinary Approach. London, UK: IWA: 211–231.
Omar, S., Rayer, S., Equaab, A., Viss, R and Steinbuchel, A., 2001. Optimziation of cell growth
and polyhydroxybutyrate accumulation on date syrup by Bacillus megaterium strain,
Biotechnology Letters. 23 (14): 1119 - 1123.
Ostle, A.G and Holt, J.G., 1982. Nile blue A as a fluorescent stain for poly-β-
hydroxybuytrate, Appl. Environ. Microbiol. 44 (1): 238 - 241.
Padmanabha, B. and Belagali, S.L. 2005. Comparative study on the water quality index of
four lakes in the Mysore city. IJEP.25:873-876.

Page, W. J and Cornish, A., 1993. Growth of Azotobacter vinelandii UWD in fish peptone
medium and simplified extraction of poly-ß-hydroxybutyrate. Appl. Environ.
Microbiol. 59 (12): 4236-44.
Page, W. J., Manchak, J and Rudy, B., 1992. Formation of poly (hydroxybutyrate-
cohydroxyvalerate by Azotobacter vinelandii strain UWD. Appl. Environ. Microbiol.
58 (9): 2866–2873.
Page, W.J and Knosp, O., 1989. Hyperproduction of Poly-beta-Hydroxybutyrate during
Exponential Growth of Azotobacter vinelandii UWD. Appl Environ Microbiol.
55(6):1334–1339.
Page, W.J., 1989. Production of poly-p-hydroxybutyrate by Azotobacter vinelandii strain UWD
during growth on molasses and other complex carbon sources. Appl Microbiol
Biotechnol. 31 (4): 329-333.
Page, W.J., 1992. Production of Polyhydroxyalkanoates by Azotobacter vinelandii Strain UWD
in Beet Molasses Culture, FEMS Microbiol Reviews, 103: 149 - 158.
Palleroni, N.J., Palleroni, A.V., 1978. Alcaligenes latus, a new species of hydrogen utilizing
bacteria. Int. J. Syst. Bacteriol. 28: 416–424.
Palmer, C.G., Muller, W.J and Hughes, D.A., 2004a. Water quality in the ecological Reserve. In:
SPATSIM, an integrating framework for ecological reserve determination and
implementation:Incorporating water quality and quantity components for rivers. Hughes
D.A. (ed). WRC Report No. TT 245/04. Water Research Commission. Pretoria, South
Africa.
Palmer, C.G., Muller, W.J., Jooste, S., Rossouw, J.N., Malan, H.L and Scherman, P.A. 2005.
The development of water quality methods within ecological Reserve assessments, and
links to environmental flows. Water SA 31 (2): 161-170.
Palmer, R.W and O’Keeffe, J.H., 1990. Downstream effects of impoundments on the water
chemistry of the Buffalo River (Eastern Cape), South Africa. Achives of Hydrobiologia
202(1): 71-83.
Panda, B., Jain, P., Sharma L and Mallick, N., 2006. Optimization of cultural and nutritional
conditions for accumulation of poly-β-hydroxybutyrate in Synechocystis sp. PCC 6803,
Bioresource Technology. 97(11): 1296 - 1301.

Paramjeet Khandpur., Jabeen, E.T., Rohini, K.V.L, Varaprasad, Y and Laxminarayana, B., 2012.
Study on Production, Extraction and Analysis of Polyhydroxyalkanoate (PHA) from
Bacterial Isolates, IOSR Journal of Pharmacy and Biological Sciences.1(1): 31-38.
Parlane, N.A, Wedlock, D.N, Buddle ,B.M and Rehm, B.H. 2009. Bacterial polyester inclusions
engineered to display vaccine candidate antigens for use as a novel class of safe and
efficient vaccine delivery agents. Appl Environ Microbiol. 75: 7739-44.
Patwardhan, P.R and Srivastava, A.K., 2004. Model-based fed-batch cultivation of R. eutropha
for enhanced biopolymer production. Biochem. Eng. J. 20: 21–28.
Peoples, O.P. and Sinskey, A.J. 1989 Poly-beta-hydroxybutyrate biosynthesis in Alcaligenes
eutrophus H16. Characterization of the genes encoding beta-ketothiolase and
acetoacetyl-CoA reductase. J Biol Chem. 264: 15293–15297.
Perret, S.R., 2002. Water policies and smallholding irrigation schemes in South Africa: a history
and new institutional challenges. Water Policy 4: 283-300.
Perry, J.J., Staley, J.T and Lory, S. 2002. Microbial Life. In: Eukaryotic Microorganisms. Adl
S.M. and Simpson A.G.B. (eds), 2nd Edition. Sinauer Associates Inc. Sunderland MA,
pp: 687.
Petersen, K., Nielsen, P.V., Bertelsen, G., Lawther, M., Olsen, M.B., Nilsson, N.H and
Mortensen, G.1999. Potential of biobased materials for food packaging. Trends in Food
Science and Technology. 10: 52-68.
Piedrahita, R.H., Zachritz, W.H., Fitzsimmons, K and Brockway, C. 1996. Evaluation and
improvements of solids removal systems for aquaculture. Aquacultural Engineering
Society Proceedings, Successes and failures in commercial recirculating aquaculture.
Northeast Regional Agricultural Engineering Service, Ithaca, New York, G.S. Libey
and M.B. Timmons, editors.
Pierce, L.2004. Natural plastics keep processor and bottlers in the ‘green’. Packaging India: 43-
46.
Popa G. Melania – Dana, 2011. “Unconventional, ecological methods of wastewater
treatment”, Summary of PhD Thesis, University of Agricultural Sciences and
Veterinary Medicine Cluj-Napoca, Doctoral School.
Porier, Y., Nawarath, C and Somerville, C. 1995. Production of polyhydroxyalkanotes, a
family of biodegradable plastics and elastomers in bacterial and plant. Biotechnology
13 (12): 142 - 50.
Porwal, S., Kumar, T., Lal, S., Rani, A., Kumar, S., Cheema, S., Purohit, H.J., Sharma, R.,
Patel, S.K and Kalia, V.C., 2008. Hydrogen and polyhydroxybutyrate producing
abilities of microbes from diverse habitats by dark fermentative process. Bioresour.
Technol., 99(13):5444-51.
Postel, S. L., 2000. Entering an era of water scarcity: the challenges ahead. Ecological
Applications 10 (4):941-948.

Postma, J., van Veen, J. A. and Watler, S., 1989. Influence of different initial soil moisture
contents on the distribution and population dynamics of introduced Rhizobium
leguminosarum biovar Trifoli, Soil Biology & Biochemistry.21: 437-442.
Pouton, C.W and Akhtar, S.,1996. Biosynthetic polyhydroxyalkanoates and their potential in
drug delivery. Adv. Drug Deliv. Rev, 18 (2): 133–162.
Pozo, C., Martinez-Toledo, M.V., Rodelas, B and Gonzalez-Lopez, J., 2002. Effects of culture
conditions on the production of polyhydroxyalkanoates by Azotobacter chroococcum
H23 in media containing a high concentration of alpechin (wastewater from olive oil
mills) as primary carbon source. J. Biotechnol., 97 (2): 125-131.
Prakash, T.W., Asman, J. and Rastogi, G.S., 2007. Water Quality Assessment of Thirparappu
Reservoir, Kanyakumari, IJEP.
Prashant A. Kadu and Rao, Y. R. M. 2012. “A Review of Rotating Biological Contactors
System”, International Journal of Engineering Research and Applications (IJERA),
(2) PP: 2149, 2012.
Pretorius, W.A., 1971. Some Operational Characteristics of a Bacterial Disc Unit, Water
Research 5: 1141.
Preusting, H., Nijenhuis, A and Witholt, B., 1990. Physical characteristics of poly (3-
hydroxyalkanoates) and poly(3-hydroxyalkenoates) produced by Pseudomonas
oleovorans grown on aliphatic hydrocarbons, Macromolecules. 23 (19): 4220–4224.
Preusting, H., Van Houten, R., Hoefs, A., Kool van Langenberghe, E., O Favre-Bulle and
Witholt, B., 1993. High cell density cultivation of Pseudomonas oleovorans: growth
and production of poly (3-hydroxyalkanoates) in two-liquid phase batch and fed-batch
systems. Biotechn. Bioengin. 41: 550 -556.
Prieto, M.A., Buhler, B., Jung, K., Witholt, B and Kessler, B., 1999. PhaF, a
polyhydroxyalkanoate-granule-associated protein of Pseudomonas oleovorans GPo1
involved in the regulatory expression system for pha genes. J. Bacteriol. 181: 858–868.
Quillaguaman, J., Doan-Van, T., Guzman, H., Guzman, D., Martin, J., Everest, A and Hatti-
Kaul, R., 2008. Poly (3-hydroxybutyrate) production by Halomonas boliviensis in fed-
batch culture. Appl Microbiol Biotechnol. 78(2): 227-32.
Radha Krishnan, R., Dharmaraj, K and Ranjitha Kumari, B.D. 2007. A comparative study on
the physicochemical and bacterial analysis of drinking, borewell and sewage water in
the three different places of Sivakasi, Journal of Environmental Biology.
Ram Kumar Pandian, S., Deepak, V., Kalishwaralal, K., Muniyandi, J., Rameshkumar, N and
Gurunathan, S., 2009. Synthesis of PHB nanoparticles from optimized medium utilizing
dairy industrial waste using Brevibacterium casei SRKP2: A green chemistry approach.
Colloids and Surfaces B: Biointerfaces, 74: 266-273.
Ramadas, N.V., Singh, S.K., Soccol, C.R and Pandey, A., 2009. Polyhydroxybutyrate
production using agro-industrial residue as substrate by Bacillus sphaericus NCIM
5149. Brazilian Archives of Biology and Technology.52(1):17-23.

Ramsay, B. A., Lomaliza, K., Chavarie, C., Dube, B., Bataille, P and Ramsay, J .A., 1990.
“Production of poly-(betahydroxybutyric- c o-betahydroxyvaleric) Acids”, Applied
Environ.Microbiol., 56: 2093-2098.
Ramsay, B. A., Saracovan I., Ramsay, J. A and Marchessalt, R. H. 1991. Continuous production
of long-side-chain poly-hydroxyalkanoates by Pseudomonas oleovorans. Appl. Environ.
Microbiol. 57(3): 625–629.
Ramsay, J.A., Hassan, M.C.A and Ramsay B.A., 1995. Hemicellulose as a potential substrate for
production of polyhydroxybutyrates), Canadian Journal of Microbiology. 41: 262 -266.
Rapport, D.J., Gaudet, C and Calow, P., 1995. Evaluating and monitoring the health of large
ecosystems. Springer-Verlag, Berlin, Heidelberg. New York, USA.
Rawate, T and Mavinkurve, S. 2001. Biodegradable plastics, Bacterial polyhydroxyalakonate,
Indian Journal of Microbiology, 41: 233 -245.
Rawte, T and Mavinkurve, S., 2004. Factors influencing polyhydroxyalkanoate accumulation
in marine bacteria. Indian J Mar Sci. 33:181–186.
Ray, D., Ravindar Rao, R., Bhoi, A.V., Biswas, A.K., Ganguly, A.K and Sanyal, P.B., 2000.
Environmental Monitoring and Assessment. 61: 387.

Reddy, C.S.K., Ghai, R., Rashmi and Kalai, V.C., 2003. Polyhydroxyalkanoates: an overview,
Bioresource Technology.87 (2): 137 -146.

Reddy, S. V., Thirumala, M and Mahmood, S. K., 2009. A novel Bacillus sp. accumulating poly
(3-hydroxybutyrate-co-3-hydroxyvalerate) from a single carbon substrate. J. Ind.
Microbiol Biotechnol. 36 (6): 837–843.
Redzwan, G., Gan, S.N and Tan, I.K.P., 1997. Short communication: isolation of
polyhydroxyalkanoates producing bacteria from an integrated-farming pond and palm-
oil mill effluent ponds, World Journal of Microbiology and Biotechnology. 13 (6): 707
- 709.
Reema Aslam, Faiza Saleem and Yasar Saleem, 2013, Biotechnological production of
polyhydroxybutyrate (PHB) from Enterobacter aerogenes, Global journal of
multidisciplinary and applied sciences, 1 (1):1 - 8.
Rehm, B. H. A. 2003. Review Article Polyester synthases: natural catalysts for plastics."
Biochem. J. 376: 15-33.
Reis, M.A.M., Serafim, L.S., Lemos, P.C., Ramos, A.M., Aguiar, F.R and Van Loosdrecht,
M.C.M., 2003. Production of polyhydroxyalkanoates by mixed microbial cultures.
Bioproc Biosystems Eng. 25 (6):377-385.

Ren, Q., Grubelnik, A., Hoerler, M., Ruth, K., Hartmann, R., Felber, H and Zinn, M. 2005.
Bacterial poly(hydroxyalkanoates) as a source of chiral hydroxyalkanoic acids.
Biomacromolecules.6(4):2290-8.
Ren, X. 2003. Biodegradable plastics: a solution or a challenge? Journal of cleaner production.
11 (1): 27-40.
Renner, G., Haage, G and Braunegg, G. 1996. Production of Short-Side-Chain
Polyhydroxyalkanoates by Various Bacteria from the rRNA Superfamily III, Applied
Microbiology. Biotechnology. 46(3): 268-272.
resources of fish. Poll. Res. (20) 2: 167- 171.
Reusch R. N., 1989. Poly-beta-hydroxybutyrate calcium polyphosphate complexes in
eukaryotic Membranes, Proc Soc Exp Biol Med. 191 (4): 377 - 381.
Rex Taicheong .Chen and Michael, K., Stenstrom, 1981. Use of the rotating biological contactor
for appropriate technology wastewater treatment, uc appropriate technology program
University of california, davis research leaflet series.
Ribera, R.G., Monteoliva-Sanchez, M and Ramos-Cormenzana, A., 2001. Production of
polyhydroxyalkanoates by Pseudomonas putida KT2442 harboring pSK2665 in
wastewater from olive oil mills (alpechin). Electronic J. Biotechnol., 4: 116-119.
Richard and Roger, 1988. Temperature effects on the growth of an obligate Psychrophilic marine
bacterium, Departments of Microbiology and Oceanography, Oregon State University,
Corvallis.
Riebeling, V., Thauer, R.K and Jungermann, K., 1975.The internal-alkaline pH gradient,
sensitive to uncoupler and ATPase inhibitor, in growing Clostridum pasteurianum. Eur. J.
Biochem. 55 (2): 445-53.
Riis, V and Mai, W., 1988. Gas chromatographic determination of poly- β -hydroxybutyric acid
in microbial biomass after hydrochloric acid propanolysis. J. Chromatogr. 445: 285–289.
Rijnaarts, H.H.M., Norde, W., Bouwer, E.J., Lyklema, J and Zehnder, A.B.J., 1993. Bacterial
Adhesion under Static and Dynamic Conditions, Appl. Environ. Microbiol. 59(10): 3255.
Rinzema, A. and Lettinga, G, 1988. The effect of sulphide on the anaerobic degradation of
propionate, Environmental Technology Letters.
Rodgers, M. and Zhan, X.-M. (2003). Moving-medium biofilm reactors. Reviews in
Environmental Science and Biotechnology. 2 (2 – 4): 213 – 224.
Rodriguez-Valera, F and Lillo, J. A. G., 1992. Halobacteria as producers of
polyhydroxyalkanoates, FEMS Microbiol. Rev. 103 (2 – 4): 181- 186.

Romano I, Haloterrigena hispanica sp. nov., an extremely halophilic archaeon from Fuente de
Piedra, southern Spain, Int J Syst Evol Microbiol. 57 (7): 1499 –1503, 2007.
Roper, H., 2002. Renewable Raw Materials in Europe- Industrial Utilization of Starch and Sugar,
NutraCos: 37 - 44.
Rosenthal, H. R, and Black, E.A. 1993. Recirculation systems in aquaculture, Techniques for
modern aquaculture. American Society of Agricultural Engineers, St. Joseph,
Michigan, J.K. Wang, editor.
Ruiz, J.A., Lopez, N.I., Fernandes, R and Mendez, B., 2001. Polyhydroxyalkanoate degradation
is associated with nucleotide accumulation and enhanced stress resistance and survival of
Pseudomonas oleovorans in natural water microcosms. Appl. Environ. Microbiol. 67:
225-230.
Rusendi, D and Sheppard, J.D., 1995. Hydrolysis of potato processing waste for the
production of poly-β-hydroxybutyrate. Bio. Technol. 54: 191–196.
Russell, J. B and Dombrowski, D.B., 1980. Effect of pH on the Efficiency of Growth by Pure
Cultures of Rumen Bacteria in Continuous Culture, Applied and Environmental
Microbiology, 39(3): 604-610.
Russell, l. B, Sharp, W.M and Baldwin, R.L., 1979. The effect of pH on maximum bacterial
growth rate and its possible role as a determinant of bacterial competition in rumen. J. Anim.
Sci. 48: 251 - 255.
Russo, R.C. 1980. Recent advances in the study of nitritite toxicity to fishes. U.S.
Environmental Protection Agency, Environmental Research Series EPA, Duluth,
Minnesota.
Ryan, P. A. 1991. Environmental effects of sediment on New Zealand streams: a review. New
Zealand Journal of Marine Freshwater Resources.
Saad, B., Neuenschwander, P., Uhlschmid, G.K and Suter, U.W., 1999. New versatile,
elastomeric, degradable polymeric materials for medicine. International Journal of
Biological Macromolecules, 25: 293 – 301.
Sabat, S., Deshpande, M.K and Khandwekar, P.V., 1998. Microbial production of Poly-3-
hydroxybutyrate-a biopolymer, Journal of scientific and industrial Research. 57: 654 -
657.
Sabhapandit, P., Saikia, P and Mishra, A. K., 2010. Int J. Chem. Sci. 8(3): 1523.
Saha, P.S., Patra, A and Paul A. K., 2005. Production of poly (3-hydroxybutyrate-co-3-
hydroxyvalerate) by Azotobacter chroococcum MAL-201, 44th Annual Conference of
Association of Microbologists of India: 80.
Saito, Y and Doi, Y., 1994. "Microbial synthesis and properties of poly(3-hydroxybutyrate-
co-4-hydroxybutyrate) in Comamonas acidovorans," Int J Biol Macromol. 16:99-104.
Saitou, N. and Nei, M., 1987. The neighbor-joining method: a new method for reconstructing
phylogenetic trees. Mol. Biol. Evol. 4 (4): 406–425.
Saleem, S., Kamili , A.N., Kakru , D.K., Bandh, S.A and Ganai, B.A. 2011 Isolation,
identification and seasonal distribution of bacteria in Dal Lake, Kashmir, International
Journal of Environmental Sciences 2(1): 185-193.
Salehizadeh, H and Van Loosdrecht, M., 2004.Production of polyhydroxyalkanoates by mixed
culture: recent trends and biotechnological importance. Biotechnol Adv. 22(3):261-79.
Sambrook ,J., Fritsch ,E.F., et al. 2001. Molecular cloning-A laboratory manual, 3rd Ed., Cold
Spring Harbour Laboratory Press, New York, USA, ISBN: 978-087969577-4.
Sangkharak, K and Prasertsan, P., 2012. Screening and identification of
polyhydroxyalkanoates producing bacteria and biochemical characterization of their
possible application, J. Gen. Appl. Microbiol. 58(3):173-82.
Santhanam, A and Sasidharan, S., 2010. Microbial production of polyhydroxyalkanotes (PHA)
from Alcaligens spp. and Pseudomonas oleovorans using different carbon sources. Afr. J.
Biotechnol. 9: 3144-3150.
Sasikala, C.H and Ramana, C.H. V., 1996. Biodegradable Polyesters. In: Advances in Applied
Microbiology, 42, Neidleman, S. L. and Laskin, A. I. (eds.), Academic Press, California:
97-218.
Satoh, H., Iwamoto, Y., Mino, T and Matsuo, T., 1998. Activated Sludge as a Possible Source of
Biodegradable Plastic. Proceedings, Water Quality International 1998, Book 3
Wastewater Treatment: 304-311. IAWQ 19th Biennial International Conference,
Vancouver, BC, Canada.
Schlegel, H. G and Gottschalk, G., 1962. Poly-beta-hydroxybuterate, function and biosynthesis,
J. Chem.74: 343 - 347.
Schlegel, H.G., von Bartha, R and Gottschalk, G., 1961. Formation and uitilization of poly – β –
hydroxybutyric acid by knall gas bacteria (hydrogenomonas). Nature 191: 463 – 465.
Schleifer, K.H., 1989. Gram positive cocci. Bergey’s manual of systematic bacteriology
Lippincott Williams and Wilekins, New York, PP: 999.
Schuster, E and Schlegel, H.G., 1967. Chemolithotrophic growth of Hydrogenomonas H16 in
a chemostat with electrolytic production of oxygen and hydrogen. Arch. Mikrobiol.
58:380 - 409.

Scott, G.2000. ’Green Polymers’. Polymer degradation stability. 68, 1-7.

Seckler D., Barker R. and Amarasinghe U. (1999). Water scarcity in the twenty-first century.
International Journal of Water Resource Development 15 (1/2): 29–40.
Seebach, D and Muller, H., 1993. Polyhydroxyalkanoates: A Fifth Class of Physiologically
Important Organic Biopolymers? Angewandte Chemie International Edition English, 32
(4):477-502.
Segura, D., Cruz, T and Espin, G., 2003. Encystment and alkylresorcinol production by
Azotobacter vinelandii strains impaired in poly-B-hydroxybutyrate synthesis. Arch.
Microbiol. 179 (6): 437-443.
Senior, P.J and Dawes, E.A., 1973. The regulation of polyhydroxybutyrate metabolism in
Azotobacter bezjerinckii. Biochem. J. 134 (1): 225-238.
Senthil kumar, B and Prabhakaran, G., 2006. Production of PHB (bio plastics) using bio-
effluents as substrates by Alcaligens eutropha, Indian Journal of Biotechnology 5: 76 -
79.
Shang, L., Jiang, M., Ryu, C.H., Chang, H.N., Cho, S.H., and Lee, J.W., 2003. Inhibitory effect
of carbon dioxide on the fed-batch culture of Ralstonia eutropha: Evaluation by CO2
pulse injection and autogenous CO2 methods. Biotechnol. Bioeng. 83 (3):312-320.
Sharmila, T., Meenakshi, S. A., Kandhyamathy, K., Bharathidasan, R., Mahalingam, R and
Panneer Selvam, A., 2011. Screening and characterization of polyhydroxybutyrate
producing bacteria from sugar industry effluents. World Journal of Science and
Technology; 1(9): 22-27.
Sheehan, G.J and Greenfield, P.F., 1980. Utilization, Treatment and disposal of distillery
wastewater, Water Res.14 (3): 257.
Sheu, D.S, Chen, W.M, Yang, J.Y and Chang, R-C., 2009. Thermophilic bacterium Caldimonas
taiwanensis produces poly (3-hydroxybutyrate-co-3-hydroxyvalerate) from starch and
valerate as carbon sources. Enzyme Microb. Technol. 44 (5): 289 - 294.
Shimizu, H., Shioya, S and Suga, K.I., 1990. Cultivation condition for production of
biodegradable polyhydroxybutyric acid (PHB) production in Alcaligenes eutrophus
H16 and Myoplans ruba R14 with respect to the dissolved oxygen tension in
ammonium limited batch cultures. Eur. J. Appl. Microbiol. Biotechnol., 7: 1-10.
Shimizu, H., Tamura, S., Shioya, S and Suga, K.I., 1993. Kinetic study of poly- D-3-
hydroxybutyric acid (PHB) production and its molecular weight distribution control in a
fed-batch culture of Alcaligenes eutrophus. J.Ferment. Bioeng. 76: 465 - 469.
Shirakura, Y., Fukui, T., Saito, T., et al, 1986. Degradation of Poly(3-hydroxybutyrate) by
Poly(3-hydroxybutyrate) depolymerase from Alcaligenes faecalis T1. Biochim Biophys
Acta. 880: 46-53.
Shivaram, S and Singh ,R.P., 2003. Biodegradable plastics Myths and realities. In Plastics for
environment and sustainable development, monograph. Thomas press India ltd, New
Delhi: 33-51.
Shrivastav, A., Mishra, S.K., Shethia, B., Pancha, I., Jain, D and Mishra, S., 2010. Isolation of
promising bacterial strains from soil and marine environment for polyhydroxyalkanoates
(phas) production utilizing Jatropha biodiesel byproduct, Int. J. Biol. Macromol.
47(2):283 - 287.
Shun Dar Lin and Dana Shackleford, B. 1986. Assessment of Nitrification in an RBC Plant,
Illinois State Water Survey Champaign.
Silva, L.F., Taciro, M.K., Ramos, M.E.M., Carter, J.M., Pradella, J.G.C and Gomez, J.G.C.,
2004. Poly-3-hydroxybutyrate (P3HB) production by bacteria from xylose, glucose and
sugarcane bagasse hydrolysate, J. Indust. Microbiol. Biotechnol. 31: 245 - 254.
Silva-Queiroz, S. R., Silva, L. F., Pradella, J. G. C., Pereira, E. M and Gomez, J. G. C. 2009.
“PHAMCL biosynthesis systems in Pseudomonas aeruginosa and Pseudomonas putida
strains show differences on monomer specificities,” Journal of Biotechnology. 143(2):
111–118.
Simon Colin, C., Raguenes, G., Crassous, P., Moppert, X and Guezennec ,J., 2008. A novel mcl-
PHA produced on coprah oil by Pseudomonas guezennei biovar. tikehau, isolated from a
"kopara" mat of French Polynesia. International Journal of Biological Macromolecules.
51(5): 1063-1069.
Singh, V and Mittal, A.K. 2012. Characterization of biofilm of a rotating biological contactor
treating synthetic wastewater. Water Sci Technol.66(2):429-37
Singh., Shree Ram and Vinod Kumar Srinivastava., 1988. Variations in water quality of Ganga
river between Buxar and Bull. Poll. Res. (3 and 4): 85-92.
Sivashanmugam, A., Murray, V., Cui, C.X., Zhang, Y.H., Wang, J.J. and Li, Q.Q., 2009.
Practical protocols for production of very high yields of recombinant proteins using
Escherichia coli, Protein Sci. 18 (5): 936-948.
Slyter, L. L., Oltjen, R. R., Kern, D. L. and Bland, U. F. C., 1970. Influence of the type and level
of grain and diethylstilbestrol on the rumen microbial populations of steers fed all-
concentrate diets. J. Anim. Sci. 31: 996.
Smibert, R.M and Krieg, N.R., 1981. General characterization, In Gerhardt P, Murray RGE,
Costilow RN, Nester EW, Wood WA, Krieg NR and Phillips GB (ed.), Manual of
methods for general bacteriology, American Society for Microbiology, Washington,
D.C.
Sofia Andersson, 2009. Characterization of Bacterial Biofilms for Wastewater Treatment.
School of Biotechnology, Royal Institute of Technology (KTH), Sweden.
Somerville, R. and Bonetta, D., 2001. Plants as factories for technical materials, Plant
Physiology. 125: 168 - 171.
Son, H., Park, G and Lee, S., 1996. Growth associated production of polyhydroxybutyrate from
glucose or alcoholic distillery wastewater by Actinobacillus sp, EL-9. Biotechnol. Lett.
18: 1229 - 1234.
Sonnleitner, B. Heinzle, E. Braunegg, a. Lafferty, R.M. (1979) Formal kinetics of poly-Jl-
hydroxybutyric acid (PHB) production in Alcaligenes eutrophus H 16 and Mycoplana
rubra R 14 with respect to the dissolved oxygen tension in ammonium limited batch
cultures. Appl. Microbiol. Biotechnol. 7:1-10.
Speece, R. E., 1996. Anaerobic Biotechnology for Industrial Wastewaters. Tennessee; Archae
Press.
Sperling, L. H and Carraher, C. E., Jr., 1986. Modern Polymers from Natural Products, in
Renewable-Resource Materials - New Polymer Sources, C. E. Carraher, Jr. and L. H.
Sperling, Eds., Plenum Press: New York: 3-28.
Spiekermann, P., Rehm, B.H., Kalscheuer, R., Baumeister ,D and Steinbuchel, A., 1999. A
sensitive, viable colony staining method using Nile red for direct screening of bacteria
that accumulate polyhydroxyalkanoic acids and other lipid storage compounds. Arch.
Microbiol., 171(2):73-80.
Squio, C. R., Marangoni, C., De Vecchi, C.S., and Aragao, G.M.F. 2003. Phosphate feeding
strategy during production phase improves poly(3-hydroxybutyrate-co-3-
hydroxyvalerate) st orage by Ralstonia eutropha. Appl. Microbiol. Biotechnol. 61 : 257-
260.
Sreya Kumbhakar., Prabhat Kumar Singh and Ambarish S Vidyarthi., 2012. Screening of root
nodule bacteria for the production of polyhydroxyalkanoate (PHA) and the study of
parameters influencing the PHA accumulation, African Journal of Biotechnology 11(31):
7934-7946.
Stamboulis, A., Baille, C., Garkhail, S., Van Melick, H and Peijs, T., 2000. Environmental
durability of flax fibers and their composites based on polypropylene matrix. Applied
Composite Materials. 7(5-6): 273-294.
Steel, K.J., 1962. The Oxidase activity of Staphylococci, Journal of Applied Bacteriology 25
(3): 445 - 447.
Steinbuchel, A and Pieper, U., 1992. Production of a copolyester of 3- hydroxybutyric acid and 3
hydroxyvaleric acids from single unrelated carbon sources by a mutant of Alcaligenes
eutrophus. Applied Microbiology and Biotechnology. 37: 1-6.
Steinbuchel, A and Schlegel, H.A., 1989. Excretion of pyruvate by mutants of Alcaligenes
eutrophus, which are impaired by accumulation of poly(Jl-hydroxybutyric acid) (PHB),
under conditions permitting synthesis of PHB. Appl. Microbiol. Biotechnol. 31:168-175
Steinbuchel, A and Schmack, G., 1995. Large scale production of poly (3-hydroxyvaleric
acid) by fermentation of Chromobacterium violaceum, technical processing and
characterization of the homopolyester. J. Environ. Polym. Degrad. 3: 243–258.
Steinbuchel, A and Valentin, H.E., 1995. Diversity of bacterial Polyhydroxyalkanoic acids,
FEMS Microbiology Letters, 128 (3): 219 - 228.
Steinbuchel, A., 1991, Polyhydroxyalkanoic acids. In: Biomaterials: novel materials from
biological sources. (Ed) D. Byrom. Stockton, New York: 124-213.
Steinbuchel, A., 1996, PHB and other Polyhydroxyalkanoic Acids. In: Biotechnology, (eds.)
Rehm H. J. and G. Reed. VCH, New York: 403-464.
Stijn Wyffelsa, Kris Pynaertb, PascalBoeckx, Willy Verstraeteb and Oswald Van Cleemput,
2003. Identification and quantification of nitrogen removal in a rotating biological
contactor by 15N tracer techniques.
Stockdale, H., Ribbons, D.W and Dawes, E.A., 1968. Occurrence of Poly-β-Hydroxybutyrate in
the Azotobacteriaceae. J.Bacteriol.95 (5):1798–1803.
Stouthamer, A. H., 1979. The search for correlation between theoretical and experimental growth
yields. Int. Rev. Biochem, Microb. Biochem. 21:1-47.
Stuart, E.S., Tehrani, A., Valentin, H.E., Dennis, D. J., Lenz, R.W and Fuller, R. C., 1998.
Protein organization on the PHA inclusion cytoplasmic boundary. J. Biotechnol., 64:
137 - 144.
Sudesh, K., Abe, H and Doi, Y., 2000. Synthesis, structure properties of polyhydroxyalkanoates:
biological polyesters. Prog. Polym. Sci. 25 (10): 1503-1555.
Sujatha, K and Shenbagarathai ,R., 2006, A study on medium chain length-
polyhydroxyalkanoate accumulation in Escherichia coli harbouring phaC1 gene of
indigenous Pseudomonas sp. LDC-5., Lett Appl Microbiol. 43(6):607-14.
Sulaiman Al-Zuhair, Muftah El-Naas and Huda Al-Hassani, 2008. Sulfate inhibition effect on
sulfate reducing bacteria, J Biochem Tech.
Summerfelt, S.T., 1996. Engineering design of modular and scalable recirculating systems
containing circular tanks, microscreen filters, fluidized-sand biofilters, cascade
aerators, and low-head or U-tube oxygenators, G.S. Libey and M.B. Timmons, editors.
Aquacultural Engineering Society Proceedings, Successes and failures in commercial
recirculating aquaculture. Northeast Regional Agricultural Engineering Service,
Ithaca, New York.
Sureshkumar, M., Mudaliar, S.N., Reddy, K.M.K and Chakrabarti, J., 2004. Production of
biodegradable plastics from activated sludge generated from a Food processing industrial
waste water, Bioresource technology, 95: 327 - 330.
Suriyamongkol, P., Weselake, R., Narine, S., Moloney, M and Shah, S., 2007. Biotechnological
approaches for the production of polyhydroxyalkanoates in microorganisms and plants -
A review. Biotechnology Advances. 25 (2): 148 - 175.
Susana Cortez, Pilar Teixeira, Rosario Oliveira and Manuel Mota, 2009. Effect of operating
parameters on denitrification in an anoxic rotating biological contactor, Environmental
Technology (30).
Susheela, A. K., 1999. Fluorosis management programme in India, Curr. Sci. (77)10, PP:
1250.
Suzuki, T., Yamane, T and Shimizu, S., 1986. Mass production of poly-β-hydroxybutyric acid
by fed-batch culture with controlled carbon/nitrogen feeding, Appl. Microbiol.
Biotechnol. 24 (5): 370-374.
Swarnalatha, P., Nageswara Rao, K., Ramesh Kumar, P.V., Harikrishna, M. 2007. Water
quality assessment by using an index at village level: A case study, Poll. Res.;26:619-
622.

Swift, G., 1998. Prerequisites for biodegradable plastic materials for acceptance in real life
composting plants and technical aspects. Polymer Degradation and Stability. 59 (1-3):
19-24.
Takeda, M., Kitashima, K., Adachi, K., Hanaoka, Y., Suzuki, I and Koizumi J., 2000. Cloning
and expression of the gene encoding thermostable poly (3-hydroxybutyrate)
depolymerase, J. Biosci. Bioeng. 90 (4): 416 - 21.
Tal, S and Okon, Y., 1985. Production of the reserve material poly-β- hydroxybutyrate and its
function in Azospirillum brasilense Cd. Can. J. Microbiol. 31: 608 - 613.
Tal, S., Smirnoff, P and Okon, Y., 1990a. Purification and characterization of D-levo-β-
hydroxybutyrate dehydrogenase from Azospirillum brasilense Cd. J. Gen. Microbiol.
136: 1191–1196.
Tamura, K., Dudley, J., Nei, M and Kumar, S., 2007. MEGA4: Molecular evolutionary genetics
analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24 (8): 1596-1599.
Tan, I.K.P., Sudesh Kumar, K., Theanmalar, M., Gan, S.N and Gordon, B.III, 1997. Saponified
palm kernel oil and its major free fatty acids as carbon substrates for the production of
polyhydroxyalkanoates in Pseudomonas putida PGA1. Applied Microbiology and
Biotechnology 47 (3): 207-211.
Tanamool, V., Danvirutai, P., Thanonkeo, P., Imai, T and Kaewkannetra, P., 2009. Production of
Poly-β-hydroxybutyric acid (PHB) from sweet sorghum juice by Alcaligenes eutrophus
TISTR 1095 and Alcaligenes latus ATCC 29714 via batch fermentation. The 3th
 International Conference on Fermentation Technology for Value Added Agroculture
Products.
Tang, Y.W., Ellis, N.M. Hopkins, M.K., Smith, D.H., Dodge, D.E and Persing, D.H.
1998,Comparison of phenotypic and genotypic techniques for identification of unusual
aerobic pathogenic gram-negative bacilli, J Clin. Microbiol.36:3674-3679.
Tanio, T., Fukui ,T., Shirakur. Y., Saito, T., Tomita, K., Kaiho, T and Masamune., S. 1982. An
extracellular Poly (3-hydroxybutyrate) depolymerase from Alcaligenes faecalis. Eur J
Biochem. 124 (1): 71-7.
Tasnim Farasat, Zubia Bilal, Fakhar-un-Nisa Yunus, 2012. Isolation and biochemical
identification of Escherichia coli from wastewater effluents of food and beverage
industry, Journal of Cell and Molecular Biology 10(1):13-18.
Tchobanoglous, G., Burton, F.L and Stensel, H.D., 2003. Wastewater Engineering (Treatment
Disposal Reuse) / Metcalf & Eddy, Inc. (4th Edition ed.) McGraw-Hill Book Company.
Thakor, N., Trivedi, U and Patel, K.C., 2005. Biosynthesis of medium chain length poly(3-
hydroxyalkanoates) (mcl-PHAs) by Comamonas testosterone during cultivation on
vegetable oils, Bioresource Technol. 96(17): 1843-50.
Thi Hang Pham., Jeremy S. Webb and Bernd H. A. Rehm., 2004. The role of
polyhydroxyalkanoate biosynthesis by Pseudomonas aeruginosa in rhamnolipid and
alginate production as well as stress tolerance and biofilm formation, Microbiology. 150:
3405–3413.
Thirumala, M., Reddy, S.V and Mahmood, S.K., 2010. Production and characterization of PHB
from two novel strains of Bacillus spp. isolated from soil and activated sludge. J. Indust.
Microbiol. Biotechnol., 37 (3): 271-278.
Thomas, M., Wright, P., Blackall, L., Urbain, V and Keller, J. 2003. Optimisation of Noosa BNR
plant to improve performance and reduce operating costs. Water Science and Technology
47(12): 141–148.
Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. and Higgins, D. G., 1997. The
clustal_x windows interface: flexible strategies for multiple sequence alignment aided by
quality analysis tools. Nucleic Acids Res 25 (24): 4876–4882.
Tian, H., Riggs, R.D and Crippen, D.L., 2000 Control of soybean cyst nematode by chitinolytic
bacteria with chitin substrate. J Nematol. 32: 370–376.
Timm, A., Byrom, D and Steinbuchel, A., 1990. Formation of blends of various poly(3
hydroxyalkanoic acids) by a recombinant strain of Pseudomonas oleovorans. Applied
Microbiology and Biotechnology. 33(3): 296-301.
Tokiwa, Y and Calabia, B.P., 2004. Degradation of microbial polyesters. Biotechnol
Lett.26:1181–1189.
Torpey, W.N., Heukelekian, H., Kaplowsky, A.J and Epstein, R., 1971. Rotating Disks with
Biological Growths Prepare Wastewater for Disposal or Reuse, Journal of Water
Pollution. Contr. Fed. 43: 2181.
Trivedi, R.K and Goyal, P.K. 1986. Chemical and Biological Methods for Water Pollution
Studies, Env. Publications, Karad, PP: 35.
Tsuge , T., 2002. Metabolic improvements and use of inexpensive carbon sources in microbial
production of polyhydroxyalkanoates, J. Biosci. Bioeng., 94(6): 579-584.
Uchino, K.T., Saito, B., Gebauer and Jendrossek., 2007. Isolated poly(3hydroxybutyrate)
granules are complex bacterial organelles catalyzing formation of PHB from acetyl
coenzyme A (CoA) and degradation of PHBto acetyl-CoA. J. Bacteriol. 189: 8250-8256.
UN-Water, 2005. Water a shared responsibility, In World Water Development Report,
Editors.

Usman Arshad, M., Nazia Jamil., Nighat Naheed and Shahida Hasnain., 2007. Analysis of
bacterial strains from contaminated and non-contaminated sites for the production of
biopolymers, African Journal of Biotechnology. 6 (9): 1115-1121.
Valappil, S. P., Misra, S. K., Boccaccini, A. R., Keshavarz, T., Bucke, C and Roy, I., 2007.
Large-scale production and efficient recovery of PHB with desirable material properties,
from the newly characterised Bacillus cereus SPV. Journal of Biotechnology, 132 (3):
251–258.
Valentin, H.E., Broyles, D.L., Casagrande, L.A., Colburn, S.M., Creely, W.L and DeLaquil,
P.A.1999. PHA production, from bacteria to plants, International: 303-306.
Van Elsas, I.D and van Overbeek, L.S., 1992. Bacterial responses to soil stimuli. In Starvation in
Bacteria, ed. Kjelleberg, S., Plenum Press, New York: 55 - 79.
Vandamme, P and Coenye, T. 2004.Taxonomy of the ge11us'Cupriavidus: a tale of lost and
found. Int. J. Syst. Evol. Microbiol. 54: 2285-2289.
Vaneechoutte, M., Kampfer, P., De Baere ,T., Falsen, E and Verschraegen ,G.2004.Wautersia
gen. nov., a novel genus accommodating the phylogenetic lineage including Ralstonia
eutropha and related species, and proposal of Ralstonia (Pseudomonas) syzygii (Roberts
et al., 1990) comb. nov. Int. J Syst. Evol. Microbiol. 54: 317-327.
Victor Chipofya, Andrzej Kraslawski and Yury Avramenko, 2010, Comparison of pollutant
levels in effluent from wastewater treatment plants in Blantyre, Malawi, International
Journal of Water Resources and Environmental Engineering (2), 2010.
Vijaya Abinaya, R., Balasubramanian, V., Ramesh, N., Natrajan, K and Rajeshkannan, V., 2012.
Exploration of polyhydroxyalkanoates production from rhizosphere soil bacteria, Envis
Newsletter Microorganisms And Environment Management. 10(4).
Vijayendra, S.V.N., Rastogi, N.K., Shamala. T.R., Kumar, P.K.A., Kshama, L and Joshi, G.J.,
2007. Optimization of polyhydroxybutyrate production by Bacillus sp. CFR 256 with
corn steep liquor as a nitrogen source. Indian J. Microbiol., 47: 170-175.
Vincent, J.M., Humphrey, B and North, R.J., 1962. Some features of the fine structure and
chemical composition of Rhizobium trifolii. J.gen. Microbiol. 29: 551–555.
Vincente, E.J., 1998 a, b. Utilizing Alcaligenes eutrophus as a source for producing
polyhydroxyalkanoates, Brazilian patent PI 9806581-5.
Vishnuvardhan Reddy, S and Thirumala M., 2012. Isolation of polyhydroxyalkanoates (PHA)
producing bacteria from contaminated soils, International Journal of Environmental
Biology. 2(3): 104-107.
Vollenweider, R.A and Kerekes, J.1982. Eutrophication of water. Monitoring, assessment and
control, Paris, OECD.

Wagner, M., Loy, A., Nogueira, R., Purkhold, U., Lee, N., and Daims, H., 2002. Microbial
community composition and function in wastewater treatment plants. Antonie van
Leeuwenhoek 81: 665–680.
Wagner, M., Rath, G., Koops, H.P., Flood, J and Amann, R. 1996. In situ analysis of nitrifying
bacteria in sewage treatment plants. Water Science and Technology 34(1–2), PP: 237 -
244.
Waino, M., Tindall, B.J and Ingvorsen, K., 2000. Halorhabdus utahensis gen, nov., sp. nov., an
aerobic, extremely halophilic member of the archaea from Great Salt Lake, Utah. 30 Int.
J Syst Evol Microbiol .50: 183 - 190.
Waksman, S.A and Fred, E.B. 1992. A tentative outline for the plate method for determining
the number of microorganisms in the soil: Soil Science. 14: 27 - 28.
Wallen, L. L and Rohwedder, W. K., 1974. Poly-beta-hydroxyalkanoate from activated
sludge, Env. Sci. Technol. 8 (6): 576–579.
Walmsley, R.D and Silberhauer, M., 1999. National state of the environment - South Africa:
freshwater systems and resources: Overview. Report prepared for the Department of Water
Affairs and Forestry. Pretoria, South Africa.
Wang, F and Lee, S. Y., 1997. Poly (3-Hydroxybutyrate) Production with High Productivity and
High Polymer Content by a Fed-Batch Culture of Alcaligenes latus under Nitrogen
Limitation. Applied and Environmental Microbiology. 63(9): 3703– 3706.
Wang, J.E and Bakken, L.R., 1998. Screening of soil bacteria for poly-hydroxybutyric acid
production and its role in the survival of starvation. Microbial Ecology. 35 (1): 94 -
101.
Wang, Y.W., Yang, F., Wu, Q., Cheng, Y.C., Yu, P.H.F., Chen, J and Chen, G.Q., 2005. Effect
of composition of poly(3 -hydroxybutyrate-co-3 -hydroxyhexanoate) on growth of
fibroblast and osteoblast. Biomaterials 26: 755-761.
Waqas Nasir Chaudhry., Nazia Jamil., Iftikhar Ali., Mian Hashim Ayaz and Shahida Hasnain.,
2010. Screening for polyhydroxyalkanoate (PHA)-producing bacterial strains and
comparison of PHA production from various inexpensive carbon sources, Annals of
Microbiology – Ann. Microbiolog : 1-7.
Warangkana Punrattanasin., 2001, The Utilization of Activated Sludge Polyhydroxyalkanoates
for the Production of Biodegradable Plastics, scholar.lib.vt.edu/theses/available/etd-
04222001.
Warwel, S., Bruse, F., Demes, C., Kunz, M and Klaas, M. R. G., 2001. Polymers and
surfactants on the basis of renewable resources, Chemosphere, 43(1): 39 - 48.

Waskar, V.G, Kulkarni, G. S and Kore, V.S. 2012. “Review on Process, Application and
Performance of Rotating Biological Contactor (RBC)”, International Journal of
Scientific and Research Publications, (2).
water quality. Supporting documentation. Ottawa.
Weibuli, C., 1953. Characterization of the protoplasmic constituents of Bacillus megaterium.
J. Bacteriol. 66(6):688-95.
Wesson, L.G., 1969.Physiology of the human kidney, New York, Grune and Stratton, PP: 591.
WHO, 1978. Regional Office for Europe, Sodium, Chlorides, and Conductivity in drinking
water: a report on the WHO working group, Copenhagen (EURO Reports and Studies
2).
Wilderer ,P.A., Bungartz, H.J., Lemmer, H., Wagner, M., Keller, J and Wuertz, S. 2002. Modern
scientific methods and their potential in wastewater science and technology. Water
Research 36 (2):370 – 393.
Williams, G and Pool, R., 2000. Composites from natural fibers and soy oil resins. Applied
Composite Materials. 7(5-6): 421-432.
Williams, M.D., Fieno, A.M., Grant, R.A and Sherman, D.H., 1996 Expression and analysis of a
bacterial poly(hydroxyalkanoate) synthase in insect cells using a baculovirus system.
Protein Expr. Purif. 7: 203-211.
Williams, S. F. and People, O. P., 1996, Biodegradable plastics from plants. Chemtech, 26 : 38-
44.
Winkler, M.A.,1981. “BiologicalTreatment of Wastewater”, John Wiley, New York.
Witholt, B and Kessler, B., 1999. Perspectives of medium chain length poly
(hydroxyalkanoates), a versatile set of bacterial bioplastics. Cur. Opin. Biotechnol. 10:
279 - 285.
Wolf, O., Manuela, C., Martin , P., Frank, M.W., Joachim ,S., Barbel , H and Gerhard , A., 2005.
Techno-economic feasibility of large-scale production of Bio-based polymers in Europe.
Technical Report Series, EUR 22103 EN2005: 81-95.
Wong, H.H and Lee, S.Y., 1998. Poly-(3-hydroxybutyrate from whey by high-density
cultivation of recombinant. Escherichia coli. Appl. Microbiol. biotechnol. 50(1): 30-3.
Wong., Phoeby, A.L., Chua., Hong and Waihung, L.O., Lawford., Hugh, G and Yu, Peter, H.,
2002. Production of specific copolymers of polyhydroxyalkanoates from industrial waste.
Applied Biochemistry and Biotechnology – Part A: Enzyme Engineering and
Biotechnology, 98(1-9): 655-662.
Woo, P.C., Ng, K.H., Lau, S.K. 2003, Usefulness of the MicroSeq 500 16S ribosomal DNA-
based bacterial identification system for identification of clinically significant bacterial
isolates with ambiguous biochemical profiles, J Clin. Microbiol.41: 1996–2001.
Wood, P.J and Armitage, P.D., 1997. Biological effects of fine sediment in the lotic
environment, Environmental Management.
Wu, Q., Huang, H., Hu, G., Chen, J., Ho, K.P and Chen, G.Q., 2001. Production of poly-3-
hydroxybutrate by Bacillus sp. JMa5 cultivated in molasses media. Antonie van
Leeuwenhoek, Int J Gen Mol Microbiol. 80: 111-8.
Xi, J., Wu, Q., Yan, Y., Zhang, Z., Yu, P.H.F., Cheung, M., Zhang, R and Chen, G., 2000. Hyper
production of polyesters consisting of medium - chain - length hydroxyalkanoate
monomers by strain Pseudonomas stutzeri 1317, Antonie van Leeuwenhoek.78: 43 – 49.

Xiang Hui Zou and Guo-Qiang Chen., 2007. Metabolic Engineering for Microbial
Production and Applications of Copolyesters Consisting of 3-Hydroxybutyrate and
Medium-Chain-Length 3-Hydroxyalkanoates, Macromolecular Bioscience, 7 (2):
174–182.
Xu, Y., Wang, R.H., Koutinas, A.A and Webb, C., 2010. Microbial biodegradable plastic
production from a wheat-based biorefining strategy. Process Biochem., 45: 153-163.
Yamane, T., 1996, Yield of poly-D(-)-hydroxybutyrate from various carbon sources : a
theoretical study. Biotechnology and Bioengineering, 41: 165-170.
Yamane, T., Fukunage, M and Lee, Y.W., 1996. Increased PHB productivity by high-cell-
density fed-batch culture of Alcaligenes latus, a growth associated PHB producer.
Biotechnol. Bioeng. 50(2): 197-202.
Yamane., 1993. Yield of Poly- D (-)-3Hydroxybutyrate from Various Carbon Sources: A
Theoretical Study. Biotechnol. and Bioeng 41(1):165-70.
Yan, S., Tyagi, R. D and Surampalli, R. Y., 2006. Polyhydroxyalkanoates (PHA) production
using wastewater as carbon source and activated sludge as microorganisms, Water
Science & Technology . 53 (6):175–18.
Yellore, V and Desai, A. 1998. Production of polyhydroxybutyrate from lactose and whey by
Methylobacterium sp. 2P24, Applied Microbiology, 26: 391 - 394.
Yezza, A and Halasz, A., 2007. Production of poly- β -hydroxybutyrate (PHB) by Alcaligenes
latus from maple sap. Appl Microbiol Biotechnol 77: 269–274.
Yilmaz, M and Beyatli, Y., 2005. Poly-β-hydroxybutyrate (PHB) production by a Bacillus
cereus M5 strain in sugarbeet molasses. Zuckerindustrie, 130: 109-112.
York, G.M., Junker, B.H., Stubbe, J., and Sinskey, A.J., 2001a. Accumulation of the PhaP phasin
of Ralstonia eutropha is dependent on production of polyhydroxybutyrate in cells. J.
Bacteriol. 183: 4217– 4226.
York, G.M., Stubbe, J., and Sinskey, A.J. 2001b. New insight into the role of the PhaP phasin of
Ralstonia eutropha in promoting synthesis of polyhydroxybutyrate. J. Bacteriol. 183:
2394–2397.
Yoshiharu, D., Akira, M., Maasao, K and Kazuo, S., 1998. Production of copolyesters of 3-
hydroxybutyrate and 3-hydroxyvalerate by Alcaligenes eutophus from butyric and
propionic acids. Appl. Microbiol. Biotechnol. 28: 330.
Young, F. K., Kastner, J .R and May, S. W., 1994. Microbial production of poly-β-
hydroxybutyric acid from D-xylose and lactose by Pseudomonas cepacia. Appl.
Environ. Microbiol 60: 4195 -4198.
Yu, H. P., Chua, H and Ho, L. Y., 1996. Microbial synthesis of biodegradable plastics. Proc
of the Asia-Pacific Conference on Sustainable Energy and Environmental Technology,
Singapore: 623 - 630.
Yu, L., Dean, K and Li, L., 2006. Polymer blends and composites from renewable resources.
Prog. Polym. Sci. 31(6): 576–602.
Yu, P. H. F., Chua, H and Huang, A. L., Lo, W. H and Ho, P., 1990 a. Transformation of
industrial food waste into polyhydroxyalkanoates. Wat. Sci. Tech. 40 (1): 365-370.
Yu, P.H., Chua, H., Huang, A.L and Ho, K. P., 1999b. Appl. Biochem. and Biotechnol., 40(1):
365-370.
Yu-Hong Wei., Wei-Chuan Chen., Chin-Kuei Huang ., Ho-Shing Wu., Yi-Ming Sun., Chi-Wei
Lo and Janarthanan, 2011. Screening and Evaluation of Polyhydroxybutyrate-Producing
Strains from Indigenous Isolate Cupriavidus taiwanensis Strains, Int. J. Mol. Sci. 12(1):
252-265.
Yung, Y.K., Yau, K., Wong, C.K., Chan, K.K., Yeung, I., Kueh, C.S.W and Broom, M.J., 1999.
Some observations on the changes of physico-chemical and biological factors in Victoria
Harbour and vicinity, Hong Kong, 1988-1996. Marine Pollution Bulletin 39: 315-325.
Zaika, T. Kot and Kharina, A. 2011. Heterogeneity of Enterobacter aerogenes
Bacteriophage Population in Sewage. 59: 29 - 31.
Zakaria, M. R., Ariffin, H., Johar, N. A. M., Aziz, S. A., Nishida, H., Shirai, Y. and Hassan, M.
A. 2010. Biosynthesis and characterization of poly(3-hydroxybutyrate-co-3-
hydroxybutyrate) copolymer from wild type Comamonas sp. EB172. Polym. Degrad.
Stab., 95 (8): 1382-1386.
Zalesny, A., Jr Ronald, S. ,Wiese., Adam, H., Wiese, B. ,. Bart Sexton, C., Richard B. Hall,
2007. Sodium and chloride accumulation in the leaf, wood, and root tissue of Populus
after irrigation with landfill with leachates.
Zhang, L., Xiong, C and Deng, X., 1995. Biodegradable polyester blends for biomedical
application. J. Appl. Polym. Sci. 56 (1): 103 - 12.
Zhang, S.M., Yasout, Lenz, R. W. and Goodwin, S., 2000, Kinetic and mechanistic
characterization of the polyhydroxybutyrate synthase from Ralstonia eutropha.
Biomacromolecules, 1(2): 244-251.
Zhang, X., Wang, Q., Liu, Y., Wu, J and Yu, M., 2010. Environ Monit Assess., 2010.
Zhao, K., Deng, Y., Chen , J.C and Chen, G. Q. 2003. Polyhydroxyalkanoate (PHA) scaffolds
with good mechanical properties and biocompatibility. Biomaterials. 24: 1041-5.

Zhao, Y.H., Li, H.M., Qin, L.F., Wang, H.H and Chen, G.Q., 2007. Disruption of the
polyhydroxyalkanoate synthase gene in reduces its survival ability under stress
conditions. FEMS Microbiol. Lett. 276 (1): 34-41.
Zhu, H., Hart, C.A., Sales, D and Roberts, N.B., 2006. Bacterial killing in gastric juice – effect
of pH and pepsin on Escherichia coli and Helicobacter pylori, Journal of Medical
Microbiology, 55:1265-70.
Zinn Manfred, Bernard Witholt and Thomas Egli, 2001. Occurrence, synthesis and medical
application of bacterial polyhydroxyalkanoate, Advanced Drug Delivery Reviews, 53
(1): 5 - 21.
Zinn, M., Witholt, B and Egli, T. 2001. Occurrence, synthesis and medical application of
bacterial polyhydroxyalkanoate. Advanced Drug Delivery Reviews. 53(1): 5-21.
Zoeteman, B. C. J,1980. Sensory Assessment of Water quality, New York, NY, Pergamon
Press.
Zulfiqar Ahmad Bhatti. 2011. “Low Cost Municipal Wastewater Treatment”, Ph.D. Thesis
COMSATS Institute of Information Technology, Abbottabad – Pakistan.