Test Preparation

Before starting the test:

Analyze samples immediately. Do not preserve for later analysis.
The sample temperature should be 25 ± 3 °C (77 ± 5 °F).
Soak glassware with 1:1 hydrochloric acid solution. Rinse several times with deionized water. These two steps will remove
iron deposits that can cause slightly high results.
To determine ferrous iron concentration, repeat the procedure, but do not add DEHA Reagent 2. Correct for the ferrous iron
concentration: OPTIONS>MORE>REAGENT BLANK>ON. The reading attributed to the ferrous iron concentration will appear.

Collect the following items Quantity

Bottle, glass mixing, with 25-mL mark 2
DEHA Reagent 1 Powder Pillow 2
DEHA Reagent 2 Solution 1 mL
Deionized Water 25 mL
Dropper, 0.5 and 1.0 mL marks 1
Hydrochloric Acid, 1:1, 6.0 N varies
Sample Cells, 1-inch square, 10-mL 2
Note: Reorder information for consumables and replacement items is on page 4.

Oxygen Scavengers
OxygenScavengers_8140_PP_2800.fm Page 1 of 4
Oxygen Scavengers

Powder Pillows Method 8140

1. Press 2. Select one of the 3. Press START. 4. Prepared Sample:

STORED PROGRAMS. tests. Fill a mixing bottle with
25 mL of sample.
When determining oxygen
scavengers that react
quickly with oxygen at room
temperature, cap the bottle.

5. Blank Preparation: 6. Add the contents of
Fill a second cell bottle one DEHA Reagent 1
25 mL of deionized water. Powder Pillow to each
mixing bottle. Swirl to mix.
7. Add 0.5 mL of DEHA 8. Press TIMER>OK.
Reagent 2 Solution to
A ten-minute reaction
each bottle. Mix. Place
period (or a two-minute
both sample cells in the
reaction period for
dark.
hydroquinone) will begin.
A purple color will develop if
Keep the sample cells in
an oxygen scavenger is
the dark during the reaction
present.
period.

Oxygen Scavengers
Page 2 of 4 OxygenScavengers_8140_PP_2800.fm
 Oxygen Scavengers

9. When the timer
expires, transfer the blank
and prepared samples into
square sample cells.
10. Immediately after 11. Immediately wipe the 12. Press READ.
transferring to the 10-mL prepared sample and
Results are in µg/L.
cell, wipe the blank and insert it into the cell holder
insert it into the cell holder with the fill line facing
with the fill line facing right.
right. Close the cover.
Press ZERO.
For greater accuracy, read
the result immediately after
the timer expires.

Interferences
Table 1 Interfering Substances and Levels
Interfering Substance Interference Levels and Treatments
Borate (as Na2B4O7) Greater than 500 mg/L
Cobalt Greater than 0.025 mg/L
Copper Greater than 8.0 mg/L
Ferrous Iron All levels
Hardness (as CaCO3) Greater than 1000 mg/L
Light Light may interfere. Keep sample cells in the dark during color development.
Lignosulfonates Greater than 0.05 mg/L
Manganese Greater than 0.8 mg/L
Molybdenum Greater than 80 mg/L
Nickel Greater than 0.8 mg/L
Phosphate Greater than 10 mg/L
Phosphonates Greater than 10 mg/L
Sulfate Greater than 1000 mg/L
Temperature Sample temperatures below 22 °C or above 28 °C (72 °F or 82 °F) may affect test accuracy.
Zinc Greater than 50 mg/L

Substances which reduce ferric iron will interfere. Substances which complex iron strongly
may also interfere.

Oxygen Scavengers
OxygenScavengers_8140_PP_2800.fm Page 3 of 4
Oxygen Scavengers

Sample Collection, Preservation, and Storage

Collect samples in clean, dry, plastic or glass containers. Avoid excessive agitation or
exposure to sunlight when sampling. Rinse the container several times with the sample. Allow
the container to overflow and cap the container so that there is no headspace above the
sample. Rinse the sample cell several times with sample, then carefully fill to the 25-mL mark.
Perform the analysis immediately.

Summary of Method
Diethylhydroxylamine (DEHA) or other oxygen scavengers present in the sample react with
ferric iron in DEHA Reagent 2 Solution to produce ferrous ion in an amount equivalent to the
DEHA concentration. This solution then reacts with DEHA 1 Reagent, which forms a purple
color with ferrous iron proportional to the concentration of oxygen scavenger. Test results are
measured at 562 nm.

Consumables and Replacement Items

Required Reagents
Description Quantity/Test Unit Cat. No.
Oxygen Scavenger Reagent Set, includes: — — 24466-00
(2) DEHA Reagent 1 Powder Pillows 2 100/pkg 21679-69
(1) DEHA Reagent 2 Solution 1 mL 100 mL 21680-42
Hydrochloric Acid, 1:1, 6.0 N varies 500 mL 884-49
Water, deionized 25 mL 4L 272-56

Required Apparatus
Description Quantity/Test Unit Cat. No.
Bottle, glass mixing, with 25-mL mark 2 each 17042-00
Dropper, 0.5 and 1.0-mL marks 1 20/pkg 21247-20
Sample Cells, 10-20-25 mL, with caps 2 6/pkg 24019-06
Sample Cells, 1-inch square glass, 10 mL, matched pair 2 2/pkg 24954-02

© Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2