OLYMPUS®

' .·
·- . i

INSTRUCTIONS
CH30/CH40
BIOLOGICAL MICROSCOPE

Th s instruction manualIs for the Olympus 8 o ogica1M

icroscope Models CH30/CH40,To ensure
the s.afety t:incJ obtainoptimum performanee andto familiarize yourself fully with the use of this
m
icroscope, we recommend that vou study this manual thoroughly before operat ng the micro·
seope.Retainth s instruct on manual in aneasity accessible plaoe near the work dcsk for future
refercnee. 11111111111111111
A X 600 0
 I M PORTA NT

.&, SAFETY PRECAUTIONS

1. Make sure that the vohage selecto< switch <D focated on the underside
of the base of the microscope trame is set to conform with the local
mains vol1age.Move the switch using an Alfen wrench,etc. (Fig. 1)
()When shipped from the factory, the switch is set to 110-120V and
230·240V in ·1OOV and 200V areas,respectively.
2. To avoid potentialshockhazard and jire,always switch 1he main switch
® to "O" <OFF) and disconnect the power cord from the wall outtet
before replacing the bulb.AUow the lamp hous ng cover@and the bulb
to cool before tooching.
3. lnstall the microscopeona level table. The air ventson the unders de of
the base shou d never be blc>c ked such as by placing themicroscope on
Fig. 1
a flexible surface,e.g.,a carpet, etc.,as this could resuh in overheating
and cause a fire.
4, Always use the power cord provided by Olympus. lf no power cordis
prov ded. p ease select the prope1power cordby referñng tothe section
"PROPER SELECT ONOF THE POWER SUPPLY CORO" at the end of
this instruction manual. lf the proper power cord is not used, Olympus
can no longer warrant the electrical safety and performance of the
equ1pment.
5. Connect the power cord cocrecttv and ensure that the ground terminal
of the power supply and that of the walloutlet are properly connected.
lf the equipment is not grounded,O lympus can no longar warrant the
electncalsafety and performance of the equipment.
6. Never insert metal objects.etc.into the a r vents of the microscope
trame as this willresult in electrical shock and/OJ personalinjury.

Safety Symbols
The foUow;ng symbols are found on the microscope . Study the meaning of the symbols. and alwavs use the
microscope in the safest possible manner.

Symbol Explenation

A lndicates thalthe surface becomes hot. and shou d not be touchedwith bare
hands.

Befo1e use,ca1efulty read the instruction manual. mproper hand ing oould
Lt. result in injury to the uscr and}or damage to the equipment.

lndicates that the main switchis ON,

1

o h dicates 1hat the main switch 1s OFF,

•
G Getting Ready

1. A microscope is a pr&e1s1on instrument. Handle il \vith Cafe

and avoid subjecting it to sudden or severa impact.
2. Do not use the m ic roscope where his subjected to direct
sunlíght, high temperatura and hum1dity,dust or vibrations.
(For operating environment conditions.refer to and adhere 10
the conditions specifiedin Section 6. SPEC
IFICATIONS on
page 16.)
3. The tenstonof 1he coarse focus adjustment knob should ontv
be adjusted by means of the tension adjustment ring.
4. Heat from this microscopeis 10dawa" by naturalconvection.
Consequently,do not use it ina oonstJicted space andensure
that tho room is well ventilated.
5. When moving the mictoscope,carry it with one hand uoder
the base© and theother hand hold ngat the recessedhandle
on the rear of the afm @as shown in theillusrration on the
Jeft. Handle 1he m1croscope vJith care when movil'lg it.
* When moving the m icroscope,never hold the stage or
binocu
lar section of the observat on tube. etc. Also
matee surethat eyep eces, specimen,etc.do not fall off.
* Sliding the microscope on the surface of thetable may
damagc or tear off the rubber feet and/or scratch the
table rop surface.

a Maintenance and Storage

1. Clean all glass compooonts by wiping gently with g.auze. To remove fingerprints or oíl smudges,wipe with gau e
slightly mo stened with a mixrure of ether (70%) an-d alcohol (30%}.
* Do not use a mixture of ether (70%) and alcohol t30%) to c eanthe lower lens (made of opticalplastic) of
the eyepiece (NCWHK10X) because suc:h solvents cloud thelens.lf dust adhetes to thelens, blow it off
or w ipe it away gently w
ith a dry cloth.
A Since solvents such as etherand a coholare highly flammable,they must be hand led carefully.Be sure
to keepthese chemicals away from openftames or potentialsources of electrical sparks -for exampte.
electrical equipment that is being switched on or off. A lso remember to always use these chemicals
on y in a well-ventilated room.
2. Do l'IOt attempt to use organic solvents to clean the noo-optica1 oomponents of the microsoope.To clean these,
use a lintfree,soft clothlightty moistened with a d luted neutral detergent.
4

3. Do not disassemble any part of the microsoope as malfunction or damage may oecut.
4, When not using the microscope,keep it covered with the provided dust cove<.

8 Warninq Labels
warninglabels are affixedat parts where specialprecautionis requiced when handling and using the microscope.
Always h-éed the warnings.

(Caution for
Warning Label Position Base Underside
Bulb Replacement l

lf warninglabe s become so led,peel off.etc. contact your localOlympusiepresentati:ve to have them replaced.

a Caution
lf the mcroscope •S operat&din a manner not spec fied by th s manual, the safety of the user may be 1mperited. n
&dditi()C'l, theequ pment may a so be damagsd.Always opefate the equlpment as outlinedinthis instructioo manual.

¡¡
 CH30/CH40
CONTEN TS

• NOMENCLATURE 1

• ASSEMBl:Y 2

2-1 Assembly Diagram ................................................................................. 2

2-2 Detailed Assembly Procedure ........................................................... 3

..CONTROLS 1

• SUMMARY OF OBSERVATION PROCEDURES 8

• USING THE CONTROLS 10

5-1 Base.........................................................................................................10
5-2 Stage ................................................................................................... 10
53 Obse rvation Tube .................................................................• ............. 11
5-4 Condenser .............................................................................................. 12
5-5 Focusing Adjustment Knobs........................................................13
5-6 lmmersion Objectives ...................................................................13
5-7 Photomicrography.......................................................................... 14
5-B Phase Contras!Attachments .......................................................16

Cll SPECIFICATIONS 17

• OPTICAL CHARACTERISTICS 18

•• TROUBLESHOOTING GUIDE
OPTIONAL UNITS
19

21

• PROPER SELECTION OF THE POWER SUPPLV CORO .................. 22

 : NOM ENC LATU RE

Eyop10C4
..
• NCWHK10X (CH30J
• WHK10X (CH40f

Observation Tube
• CHl Bl45
• CH3-TR 45
• CH3-M045

Microscope Frame
• C>llORF
• C>llOlF
• CH..iRf
• CH<OlF

Objectiva
• EOA 4)(, 10X,40X,
100X oíl

Condensar

[g:: º]
BH2 PC
BH-OCO
CH3 COP
 AS SEM BLY

2-1 Assembly Diagram

The diagfam below shows how to assemble the various components.The numbers indica e the order of assembly.
* When assembling thé microsoope, make sure that all parts are free of dust and dirt, and avoid scratching
any parts or touching glass surfaces.
* Retainthe prov ded Allen wrench .lt shou d be used when units are replaced or added.

Cotd rest ·
(optional for CH30/CH40)

Objectlve

Condenser Field iri$ tJittphragm

tittaehment
(optionalfor CH30>

81ue fi ter
•

Filter ho der Hor CH30t

11 • •
6V,20W halogcn bulb
<forCHJlll ti3J!'
cz:P'
6V,30W halogen bulb
(for CH401
 CH30/CH40

2-2 Detaikd Assemb Procedure

a Mounting the Bulb (Replacemenl) tFig.1)

1.Tum the moaosccpe trame onrts s.ieand pu the lomp ho•JS">g knob
© on the unc1e1 .1e o4 the base to open the lomp housing OOYer.
2. the nologen bulb®contaoned on the polyethylene bag to aYOÓd
leaving f1ngerprin1s on the bu-lb,fu
ly insan thecontact pinsinto 1he
bu boockolQl.When properly S4310d, pull off the polyo1hyléne b•g.
(( Appllcable Bulb))
CH30; fN, 20W Halogen bulb:6V20W HAL IPtiillps 7388)
CH40; fN, 30W Halogcn bu b;6V30W HAL IP!i;fips 57611
Fog , 1 A Always use the designated bulb.Use of lmproper bulb may
result infir1.
A Do not touch the bulb with bare hends. lf tlngerprints a.re
aocidentalty lefl on the butb.wipe the bulb with a soft. lint·free
cloth moistaned with alcohol. Using a contamlnated bulb will
shonen thasarvlce l fe of the bulb.
3. With the lamp t\ous1ng knob still pulled out. close the lamp housang
oover. Thetn pushin thelamp housing knob co lock the oover.
* The lnmp houslng covercannot be closed lf tho knob is pushed
In before you iittempt to close covor.

Prec.aution.s for Halogen Bulb Replecemen1

A. Whenever you replace the bulb during use or right'after use,
firs1move the mainswitch to o ·«OFF},diaconneQ the power
cordfrom the wall ouUet.and allow the bulb 1nd pans around
the bulb 10 oool before touching.
* lf the bulb burns out during an observation and needs to be
replaoed, remove eyepieices, specimen,andother objects likely
to fall off, before tílting the microscope frame to replaoe the
bulb.

a Mounting the Observation Tube tFig.2)

1. UStng the provided AJlen wrench.k>osen the obsetvauon tube

ctampong ser-(!).
2. lnsert the orcull' dovetai mount at the bottom ot the observation
tube into 1he open1ng on the m icroscope frame.pos11ion n9 the
observat1on tube to peint the binocu ar eyopieoas towards the front.
Clamp the observat•on tube by tightenrng the clamp1ng screw ©·

Fig. 2
 11 Mounting the Eyepieces (Fig.3,4>

Binocular Tube (CH:l-8145),Monocu ar Tubc {CH3 M045) 1

t . Remove the eyepiece dust cap©. (Fig.3)

2. lnsert theeyepiece(tl nto the eyepiece sfeeveas far as it will go.
(Fig. 3)
3. Moont the other eyepiece in the same manner.

Fig.3
,. - J Uslng Eyepiece Micrometer Discs (optionaO 1 - - - ... - - - - .,
Eyepiece m
icrometer discs can beinserted into the NCWHK1OX
eyepieces.Use 1O mm/100 mic<ometer discs.
1. Unscrew the micrometer mount1ng frame @ from the eyepiece
by turning counterc ockwise.
2. Place a micrometer disc into the trame. The engraVing on the
micrometer disc should tace downwardinto the micrometer
mounting frame@.CF g. 4)
3. Screw the micrometer mounting frame backinto the eyepiece
tube.(Fig.4>
* Use 19 mm diameter micrometer discs.
Fig.4

CI
Mounting the Objectives {Fig. 5)
High Mount lhe objectives on the revolving nosepiece in such a manner
magnlfl·
c.;11ic)n that the magnificat on increases fromlow to h gher powers in a
counterclockwise d rection.
O lt there are empty sockets with no objective mounted,make sure to
Low
attach the provid&d dust plugs. ·
magnilie81ion

Fig.5

Cil Mounting the Condenser !Fig. 6)

1. Turn the coarse ad ustment knob © to raise the stage 10 its high&st
position.
2. Tum thecondenserheight adiustment knob@tok>wer thecondenser
holder to the position "vhere the clamp ng screw @ can be tui-ned.
3. lnsen the condensar © into the mounting hole ® and clamp by
tightening the clamping screw@. Position ngthe oondense1with the
diaphragm lever@ at the front facilitates operation.
4.Turn thecolldenserhe1gh1adjustment knob@tota se the.coodénset
holder to its highest position.
Fig.6
 CH30/CH40

a Mounting the Blue Filter (Fig.7)

OThebfoe fittet convens the cOlor temperatute of tungstcn illuminauon

into the color temperatureof daylight toshow the spacin n io oa1ural
colors.
Place the 32.5 mmdiameter b ue f lter @in the ftltet h01def CD or the
compensating lel"IS.

F g.7

8 Mountingthe Fliler Holder or the Compensating Lens (F g.8)

1.lnsertthe filter holder or the cO«'lpensalinglens ©into the condensef

ffom below untilit seats w ith ciick.
2. When mounting the oompensat ng lens.place the lens so that the
centeñng screws ® are at tl'le front.

Fig.8

a Mounting the Field Iris Oiaphragm Anachment

(CH30 only) (Fig.9)

Holding the fieldirisdiaphragmatt&ehment@over 1he light glass exit

<D with 1he rilter holder notches @ positioned as shownin F g.9,
inse-rt the atta'chment into the light glass exit opening unt l.it seats
wi1h a click.
* Attempting to insert the attachmcnt with the notches@ facing
inother dlrections than shownin Fig.9 mav break the mount-
ing clip.

Fig.9

C!I Connecting the Power Cord (Figs.10

,11,12)

A Cables and cords are vulnerable when bent or twisted.Never

subj ect them to excessive force.
A Make sure that the main switch © is on "Oº' (OFF} bef ore
connecting the power cord.(F g.10)
.k. A Always use the power cord proyided by Olympus.

u lf no power cordis prov ded,please select the proper power

cord by referring to the sect on "PROPER SELECT ONOF THE
POWER SUPPLY CORO" at the end of this instruc1ion manuat

Fig. 10
 1. Connect the power co<d plu9@ to the AC receptacle @.lF g. 11)

Fig. 11

2. P1ug the power c0<d plug © into a wall outlet. (Fig. 1'2)
A Connect the power cordcorrectly and ensure that the ground
terminal of the power suppty and that of the wall out et are
properfy connected.
lf thc cquipmcnt is not grounded,Olympus can no longer war·
rant the etectr calsafety and performance of the equlpment.

Fig. 12

cm Cord Rest (CH3-CH) (optional) ffigs. 13,14)

O V\'hen the cord rest (CH3..CH)is anached on the rear panelof the
microscope frame,the po...ver cord can be \YOund around it for
s1orage.
Alig.n the hooks @ on the cotd rest © with the air vent s its at @.
and insert the hooksinto theslits unt lthecord rest is pressed aga nst
the rear panel of the mic roscope trame .Then pu11 the oord rest down
to secura it.
Note:The illustration of thecord rest only shows thesections íelevant
ig. 13 for 1he explanation here.Theactualcord rest looks somewlla1
dJfferent.
* Do not grasp the cord rest when moving the microscope .The
cord rest may suddenly come loose and you may drop the
microscope causing material damage or personal injury.
Oetaching-Move themicroscope trame tothe edge of the table@.
Then apply a screwdnver@Or' Allan wn: ncl\ 10 thA 10..ver port en of
the cord rest © and pushin the OJ m directions to move the enti'e
cord rest upward to allow it to be detached.

Fig. 14
 CH30/CH40
CONTROLS

lnterpupillary d
istance adjustment se.ale

Diopter
adiustment ring

Main switch

Light intensity
knob

V-ax s knob

X-ax is knob

Apenure iris diaphragm knob

Filter holder For 45 mm

diameter filters Fine focus
etdjustment knob
Coarse focus
adjustmeqt knob
Coarsc adjustment
knob tension
adjustment ring

•Fie diris diaphragm dial

ll The CH30 is not equ1pped wnh the field iris diaphragm funct on but an op1ional field ifis
diaphragm attachment canbe mounted.
 g SUM MARY 0.F OBSERVATION PROC EDURES

-- Q•
•
1. Switch the main sw
itch to "I" (0NJ and adjust the br ghtness with the
light ntens ty knob.(Page 101

·. .
2. Turn he revolving nosepieoe to engage the 1OX objective. Make sure
that the revolving nosepioce stops with an audible cl ck.

(/)
w
a:
3. Place a spec men on the stage. f Page 10}
=>
o

z
o

w
g¡ 4. Tun\ the X-axis knob © and Y-axis knob@ to move the specimeninto
o
... the light path.(Page 11)
o,_
a:
:::¡

5. Looking throughthe right eyepiece withyrtight eye,t1.1rn the coarse

adjustment knob to bring the spec men into focus.
After obta n ng approx mate focus,use the fine adjustment knob to
make fine adiustments.

6. Looking through the left eyepiece with your left eye,turn the diopter
adjustment ring to focus the specimen.(Page 11)
 CH30/CH40

7. Adjust the interpuptlltrv distante of the eyepieces.(Page 111

8. Center the f191d 1ns dlaphragm by maniputatlng the two centeringsaews

on the compensati ng Jens.lCH40 onlyl (Paoe 121

..
In e.ase of CH30. cent'8tioo is not neces.sary. lf the optlonal field iris
diaphragm attachment is mounted, center the at achment in the same
manner as describad above.

9. Engage the ob¡ectlY9lO be used for observation and ad ust tlle r111ht in-
tensity to the desired level. then readj ust the locus.

10. Ad ust the field oris doaplvagm.

Thls only applies to the CH30 if the optoonal field iris diaphragm attach-
ment is mounted.

11. Ad¡ust the rture 1ns dlaphragm. (Page 121

1
USING THE CONTROLS

a Turning ON the Bulb (Fig.15)

1. Switch the main switch © to " I" (ON>.

2. . Turningthelight intensity knob@cloOk\vise in the direction of higher
numbe1s makes the illum inatjon brighter,and vice versa.

Fig. 15

fJ Field Iris Diaphragm (CH40 only) {Fig. 16)

Using the field

iris diaphragm dial©.adjust tho diameter of the field
iris for objective power to the extent that itjust circumscribes the f eld
of view.When the fieldiris diaphragm is stopped down to c rcu1n
scr be the field of view.ll will exclude extraneovslight and improve
image oonvast within the field of view.
* When using 100X objcct ve, thefleldiris diaphragmimage will
not be visib e within the fie d of v ew.Accordingly,stop down
the diaphragm toits sma11est diamet er.
O In case of CH30,the f1eld iris diaphragm anachment is mounted on
Fig. 16 the light exit glass of the microscope base.

a Specimen Placement .<F g. 17)

* Releasing the curved finger with great force,or suddenly re-

leas ng your griponthe curvedfinger knobwhife re easing the
curved finger, w ill crack or damage the slide glass.A lways
release the curved flnger with great care.

Specimen Holder for Single Slides

1. Tvcn the coarse adjustment knob CD counterclockwise in the direc·

tion of the arrow to lower the stage.
Fig. 17 2. Open the spring-loaded curved finger®on the specimen holder and
slide the spec1men s ide into the spec men holder from the front.
3. Alter plac ng the slide as far as i1will go,gently ralease the curved
finger(

Specimen Holder for 2 Specimen $lides

1. Place the first spec men slide as described in steps laod 2 above.
Then place the second spec men sl de so that t contacts the firs1
speoimen -$lide.
2. Gently tetease the curved finger ®·
Plecing the Specimen Slido with One Hand
Piece the spec1man sl de at 1he front of t.he stage. then shde the specimen shde on the stage surlaoe to slo\vty and
gradually open the curved f1nger in the d1rect1on of the arrow. nsert the specimen sl•de 1nto the speomen hokSer
unul111s fu ly and properly sea1ed in 1he specimen tiolder.
Cover Glass
Use cover glasses of 0.17 mm th1ckness in combinahon wnh objectives marked wi h the •160/0.17.. engraving for
opt1mum performance of these objectives.

Specimen Slide 1
Spec1men slodes w11ha thookness between O 9 mm and 1.2 mm are rocommended le< CH30/CH40 m1crosropes
Us1ng1hi(kef specmenshdes may p<event cOfrect formattOn of thefieJdiris diaphragm image onthe specimensuñace
ination w111 not be correct in case of darkfietd observation.
end illum

fl Moving the Specimen (Fi9.18)

Turn1ng th& Y..axis knob <!> moves tho spec1men in the Y axis direc·

-
tion. Turn1ng the X·axis knob @ moves the spec men in the X-ax.is
directJon
* Never move 1he specime.n by hotding the specimen holder or
stago directty as th s wHI damage the knob rotationmechan sm.
• Whan further movement of tho stage or speclmen holder is e
(/)
prevonted by theprov ded limiting mechanism, the tension of z
the Y·axis and X·exis ltnob rotation wlllincreaso.At this point, G>
do not rotate the knobs further. App
lyingexcess vo force cou d m
destroy the limiting mechan sm.

S.J
Frg 18 o

--
o

Observation Tube (CH3-Bl45 ) ¡¡;

a Adjusting the lnterpupillary Dístance (Fig.19)

While look1ng 'hrovgh tho eyepi es.adjust for bfnocutar v1s1on unhl
the left ond righlfields of view coincide completely.
Adjust so that the twO 1ndox dots • are hOritontaL

Fig.19
0 Placing the 1nterpupillary distanceindexes so that they are parallel
wnhthe h0<1zon1aline$ fac1buues the edtU$tmenL
Also l(;eep the dots parallol Vlrf'lth the horizontal linos 1n case of
1nterpvp1llory CfiSlances 01her 1han 50.60. 70. and 75.
O Note vovr ln1orpvp1llary dls1ance so 1ha111 can be qu ckly dvplica1ecr

fl DiopterAdjustment (Fig.20)

1. Look1ng through the right evepiece W11h your right eye,totate the
coarse and fine adjustment knobs to br1ng the specimen lnto focus.
2. Look ng 1hrough theleft eyepiece wi1h your ef1eve. turn 1he diopter
Frg 20 ad1ustmen1 nng © to focus 1he speamen
 5-4 Condenser

a Centering the Field Iris Diaphragm (CH40 only) (f igs.21,22)

1.With the 1OX objective engaged andthe specimenbrooght into focos.

turn the fieldiris diaphragm dtal© counterclock-wise to stop down
the diaphragm somewhat.{Fig.21>
2 Tufn the condenser height adjustment knob @ to bring the field iñs
d aphra9m ima9e into focus.(Fig. 211
3.While gradually opening thefietdiris diaphragm image,rotate the two
centering knobs@of thecompensatinglens to adjust so that the field
ir s diaphragm image is centered
in the eyepiece field of view.
(Figs.2 1,221
Fig. 21

·O
Fietd iri$ 4.To check centrat on, open the fieldiris diaphragm until itsimage
d iaphrag m lmage louclles the perimeterof the field of view. lf the image is not prec sely
inscribed 1n the field of view.center again.{Fig.22)
5.Whenused for actualobserva1ion.open the field ins diaphragm unt l
its image j ust c rcumscribeslhe field of v ew.

(/)

6 mountedon the CH30,center the diaphragm in the same manner as

a: Evepieice
O Whenthe compensat nglens and fieldiris dfaphragmat1achment are
field of view
o
Jw: Fig.22
.....

a
(!)
z Aperture ris
¡¡; d1aphr.,,gmi tnBge ApertureIris Oiaphragm lFigs. 23, 241
;;¡
7()-00%
O The aper1ureir s diaphragmdetermines the numefic-al aperture of the
30-20% illumination system. Matching the numericalapenure © of 1he
illumiriat•on system withthat of the objec t ve provides beuer image
reso ution and contrast,and atso increases the depthof focus.
O Since the contrastof m icroscope specimens is ordinarily low.sening
the condensar apertura iris diaphragm to 70-80% of the N.A .of the
object vein useis usualfy recommellded.When necessary,adjust the
Obfeef ve pup.il
rat o by removlng theeyepiece andlookingintothe eyepiece s eeve
while adjusting the aperture ins d1aphragm lever @ until the image
Fig. 23
shownin Fig. 23 is sean.

Fig.24
 CH30/CH40

5.5 Focusing Adjustment Knobs

a Adjusting the Coarse Adjustment Knob Tension (Fig.251

1. The coarse adjustment knob tenslonis preadjusted for easy use.
However.tt desired.one can change the tenSIOl'I US1ng the 1ensl0rl
a<11ustmen1nng (D.Applying a flal bladed screwdriver 10 any of the
grooves @on the c1rcumference of the rin9 and turning the ring in
the direct on of the arrow increases tension,and vice versa.
2. The tenslOrl os too low rt the s age droPS by n.11 or focus is quicldy
k>s1after &d1us1ment W'fth the fineadjustment knob@In this case.
turn the nng © 1n the d1rection of the arrow toincrease tension.

F1g 25 fl Pre.focusing ever (fig.26)

The pre focus1ng leve1 ensures that the objoctlve does not come in
oontact w1th thespecimen and s1mplihes focu.sing After focusi.ngon
lhe speomen WJth the coarse adiusunent knOb, 1utn thls '8-ver ©

-
ckx:kwise 1n the direc\JOn of the arrow 10 set a lower lim1t on coarse
8dJUS tment 1novement .
O Focusi11g with the fine adjustment knob is not affected by this pre
tocusinglewr Aceo<d1ngly.alter us ng the coerse adjustmenlknob
to tower thes1age for Chang:ing spec1mens or appfy1ng immersion oil e
(see Sect1on 5-6). re1ocusing is eas1ly accomphshed by rotating the (JJ

coarse adjustment knob 10 reach the pte-focus1ng posítion, then zQ

ma)::mg fine adJUstments wnh the hne adJustment knob.
• When not required, ea.ve tho pre 'focusing lever unlocked.
=i!
Fio.26 m
8
o
a Use of tmmers ion Objectlves
1.Focus on 1he specimen W1th a low power obiectJVe.
(f lg. 27)
!ñ

2 Place a drop of 1mmerscm 011fprovided) ontothe speamen at tl1e area

10 be obserl/9d.
3 urn the revolving nosepiece to engage the lmmersion objective.
menfocus uslnglhe hne adjusimen1knob.
• Sinceany bubb&es in me oilwill mpair the image.mako sure tha1
! e on is free of bubbles.
h
o. To checlc for bubbtes. remove the eyepiece and f ully openlhe
field and epetture iris d1aphrsgms. then look at the ex1t pupil of
the ObJeCliveins1de the observation tube.ffhe pupll shoukj
appear round and brtghtJ
F1g 27 b. To remove bubbles. roc:t< the nosepieoe shghtly to move the 011
1mmersion ob ect ve back and forth a few times.
0 lf the condenser marking shows a nt1merical aperture tNAl of 1.0 ot
more.the number appl1Gs only-oil is present betw<l80 the sltde
gtass and the top st1rf of tlle condenser.When oif 1s not present.
!he NA is ab0u10.9.
4. After use, remove oilfrom the ob ¡ective front lens by wiping with
gauze slightlv mo1stened with an e1her (7 perts) / alcohol 13 parts)
mucture or w1th EE-6310.
• Using too much alcoholcan dlssotve the lens adheslve.
A Ceut on on Using tho lmmerslon Oil:
lf theimmersion oíl comesinto contact wíth your eye or skln.
immediatety take the following aetion.
For eye:Rinse w
ith ctean water (for more than 15 min.}.
For skin:Wash wit h aoap and wetor.
lf tho appearance of tho eye or skln changes or pain continuas,
pfease consult your doctor.
 OMcam1r11 back

1 Automatlc: photomicrogf•phic sys,tem 1

Ff Photomicro ad&pter L
PM
·JO
Autom tio photomicrogr•phlc syshrfn 1
MOM ·L
PM·20
1
...__ _+
1 -----------'l Automatic exposu1e photomictographic ivttem 1
- ( PM -10AK
Pho10 cyepiece
8 NFK

cQ NFK photo eyepiece

O adaptar
PM·AOF
1

:.vr-... .-, Trinocular obMrvation

IUbe
CH3 TR'5
O Use the tr nocular obsorvatt0n tube CH3-TR 45 for photom1crography
* Use a heat absorbing íllter C 45HA> when using the PM-10,PM-20,and PM·30 photomicrographic systems.
a Selecting the Líght Path (CH3-TR45) (Flg. ZB)

O The interpupiltary distance adjustment procedure of the trinocular

obselllatJon tube (CH3-TR45) is dofferent from that of the binoc:Uar
observa•ion tube (CH3 8145) The interpupdlary distance doveta•I
slides (j)on thB CH3-TR45 sllould be moved to make the adjustment.
Movlir Ou:1light pathselector knob<l) toseleclthe requ1redlight path.

Fig.28
n O Far normalobservallOfl.!helight pacllselector l<nOb shouldbe pusl1ed"'·
For TV and stI photomicrography.pul out the knob.

Ligh1 palh tielocto1koob lntenshy ratio

·

Appl c&t!on

Pushed 1n Observat>On of dafk

Puíled OUI
............
1 0 0' * ' fO f binoo.Jf.at

20% fOl binoculaf

"'8cirneO>
Observation of bnght
evepieces.80% for specimons.
TV/phOtomicrogtaphy photomicrograi:ihy,
TV obse<vtt>On

•In case of long time exposure.ambiont light in tho room will

ontar through the obsetvotlon tube and the eyepiocos and may
ereate ghost mages or fiare.To block this extraneou1light dim
the room or cap Uie eyep
ieces or the focusing telest:ope.
 CH30/CH40

fl Mounting the Camera Unit (F g.29}

l. loosen the clamping screw ® of the NFK photo eyepiece adapte(

(PM-ADFl© .
2. Attach the NFK photo eyepieoe adaptar (PM ADF) © tothe trinocular
observation tube (CH3 TR45) @and tighten the clamp ng screw@,
3, lnsert the NFK photo eyepiece (3.3X or 5Xl ©into the NFK photo
eyepiece adapter <PM ADF)©.
4. Mount the camera unlt directly on the NFK photo eyepiece adaptar
(PM ADFI(j).

Fig.29 Using an DM Camera Back 1

Touse an OMcamera back, mount the photo eyepiece adaptar PM-
ADF © (into wh ch a choice of NFK photo eyepiece isinserted),the
photomicto adapter MOM-l® and the OMcamera back onthe photo
tube in the described orde<.
• Use the 3.3X or SX NFK photo eyepiece.
• Focus using the indar of the camera back.
• Photomicrographic magnificat on
= Objeciive magnification x Photo eyepie-ce magnification.
* To take a good photomicrography w ith a single lens reflex e
(J)
camera back.. a shutter speed at 1/2 second or slower is rec-
ommended so as to reduce the mirror-lcxkup shock. z
Gl
:i!
m

11 Focus Adjustment g
The focus of the eyep eces and the camera f lm plane are not the o
same. Consequently,always perform camera focus ng by using the ¡¡;
focusingte!escopeof the camera unit.For detaits,refer to the
instruc·
t on manual of the photomicrographic system.

a Color Temperatura Compensation (fig. 30)

OWhen using daylight type film in the camera.

* The bluelf filthis
ter is sole
filter isyengaged
designedwhlle
for visual observat
taklng on {day-with
photographs
light).
daylight film,the color reproduction will be poor.
1, Mount the light balancing filter (45LBD 2N) © in the filter holder@
of the m
icroscope frame.
2. Turn the light intensity knob @.To obta n illuminat on clase to day·
light, set the index of the knobat 5 incase of the CH30.and 4 in the
case of CH40,
Fig. 30
 5-8 Phase Contrast Attachments
a Nomenclatura

! L ght Annuli 1 Green F lter

45G533

CH2 RS10 CH2·RS40

1 Phaso Conrrast Objectives PCDA10X PVPCDA40X PL 1 1 Centering Telescope CT S 1

•
da:
§ G
.,
Mounting the Phase Contrast Attachment
w
..
J ::
(!)
z
Mount the phaseannulus (CH2-RS 10/CH2-RS40) in the same way as the filter holder.
* Replace prev ous y mounted objectives witllthe phase contrast object ves.

a Using the Phase Contrast Attachment (Fígs.31 & 32)

Before oentra1íon AfUtr oont1<11ion

1. Tutn lhe revolving nosep1ece to move the ObJéCtive w1th the same
nvmber as the mounted light annulus. ·
2. Set the specimen on the stage and br ng it into focus.
3. Remove either one of 1he eyepieces and insen the centefln9
telescope <CT-5> into the eyepiece tube.
4. Rotate the CT s·s upper lens assembfy so that the b<igh1ring © Uight
annulus in the condensen and !hedark ring @(phase annulusin the
obiecttve) come in10 focvs.<Fig.31)
Fig.31 5, Rotatethe twolight annulus oentering knobs@untilthe light annu us
aod phase annulus ate conceotric and supet mposed.(Figs.31 & 32)
6. Aemove the CT-5 and insert the evepiece back 1nto the eyepiece tube
to perlorm phase contrast observation.
7. To increase ima9e conuast.movnt the f ltar on the fllter holder.

Fig.32
 CH30/CH40
SPECIFICATION
S
Speeifications
lteM
CH30 CH40
Op1icalsystem LB optlcal svstem (f1nitorrected system)
Ulumi.nat1on muminator butlt-in
fN 20 W hllogen l>Jlb Ph
ips 7388 fN 30 W halogen bulb Pholops 5761
IA-oge senoce time:App<oxrnately too tws (Average service bme:Appfounatety 100 hrs.
wllM usod as diroctedl wl>en used as directedl
CH30RF100/LF100: 1oot1t o-t2ov.50/60 Hz CH40RF100/LF100 : 100/t 10-120V, 50/60 Hz
0.3A 100-120V or&a 0.42A 100-120V orea
CH30RF200/LF200: 220/230 240V,50/60 Hz CH40RF200/LF200: 220/230 240V,50/60 Hz
O. SA 220-240V are• 0.21A 220-240V area
Focus ng Steoe movement by ro!ler guide (Rack & Ptnlon>
Stroke per rotation:39
.6 mm
Fullrange stroke:25 mm
Upperl1mllstopper
Torquead ustment on ooarse focus knob
Rewlving Bullck>n qua<lruple nosepoece Build-on <µntuple noseooece
nosep;ece with 1nward tilt with inward 1J11
Observa1ioo rube MOllOCUlar Tube inchnat.on 45'
CH3 M045
B
inocular Tube inclinatton 45'
CH3-8145 lnterpup1llory distanc::e adjustment 43-75 mm
Stage Sito 188 mm x 134 mm
Movement 76 mm X-dlrection X 50 mm Y-directJOn
$J*l men holder Double shde hokler
Condense< Type Abbe condense<
NA 125 (with oíllmmerslOfll
Aperture ...,diaphragm PrOll1dod
Dimen...,•and ht 233(Wlx3921H)x301CD) mm;a 2 l<g (1811>1!CHJOW85 <o11a1lll)!CH40>

Operat ng •lndoor use

environment • Allitudo: Mex.2000 m
• Ambient tempera1ure: se to 4o•C( 41• to l04°F)
• Maxlmum relative hum1dity 80% for temperaturas up to 31.,C 188"'F) decrees1ng linearly
through70% et 34ºC t93•A. 60% ot37"C !99"FJ.to 50% relative
hum.duy at 40'C !104°FJ
• Mein supelfy vohage ftuc1uab0f\S not to exceed '% 10% of the nominal volt.IQI.
• PoluuonOegree 2 (In .ccordance wtth IEC 664)
• lnstalla1k>n/Owrvottage categoty lihn occordance wíth IEC 664)
 OPTICAL CHARAC1'ElJ STJCS

Magn ficatlon

tmmersion object ve
Nt1me1calaperture

Cover glass 1htcknC$$

Mechanicaltubelength
Color band

CH30
Eyep1ece
N.A. w.o. R13$0IU • NCWHK10X (FN. 18)
' Magni·
fieation (mm) tion Remarks
I (µm) Total F eld of
' Depth of
e mag. f(o¡cus view
.m)
EDAdl 4X 0.10 29 3.4 40X 175 4.5
Achromat IOX 0.25 6.30 1.3 100X 28.0 1.8
40X 0.65 0.53 0.52 400X 3.04 0.45 Spnng-k>aded
100X 125 0 20 o 27 1000X 0 66 0 18 Spring- ded
Oil imrnerSJOn

CH40
Eyepiece

s
w.o. Reso
lu- WHK10X {FN.20)
Magni- N.A. Remarks
tion
r fication (mm)
(p.m) Total Oepthof Field of
• mag. focus
(µm) view

EDAoh 4X 0.10 29 3.4 40X 175 5.0

Achromat lOX 0.25 6.30 1.3 100X 28 0 2.0
40X 0.65 0.53 .52
0 400X 3.04 0.5 Springloaded
IOOX 1.25 0.20 .27
0 IOOOX 0.66 0.2 SQnng-loaded
Oilimmerslon
• The resolving po..,.1er of immerst0n objectives is obt ined wit hthe apertura iris diaphragm fully open.

Glossary 1

Worl<ing distance fW.0.):
Nume ical apertura (N.A.>:
Resolving power:
Focal dépth:
Field number:
Field of view diameu r:
Total magrufication:
The distance f rom the cover glass surface 10 the nearest point of the objective.
The N.A .value tepresents a performance numboí wh ch can be compared to the
íelative apetture (f·number) of a camera lens.Th& higheí N.A.• the higheí th& íesotving
P0\V9r.
The abi!ity to differentiate two points,i.e.,the minimum distanoe by wtiich the objects
must be separated in order to be revea ed as two separate objects .
The depth in the image. throughwhich thefocused image willappear unif ormty sharp.
As the apertureiris diaphragmis stoppeddown. the focal depth becomes greater.The
gíooteí the N .A. of an objective,the shot1er the f0<:al depth.
A number lhat represents the diameter 1n mm of the 1mage of the f1eld d1aphragm
that is formed by the lens in front of it.
The actual size of the field ot viewin millimeters.
Equa
ls the objtive magnificat on mult plied by the eyepiece magnification.
 l
CH30/CH40
o TRO UBLES HOOTI NG GUI DE
Under cenain cond tions. performance of th1s untt mav be adversefy attected by factors othof 1hen delects.
lt problems occur,pleaseieview the toUowmg hst and take rornodialaett0n as appropnate .lf you eonnot solve the
problem after checking the entire L st. please cont&e1 your local Olympus representauve for ass1s1ance.

Probl.,...
1.Optlcet System
Ceute
-
a, Fieklof \/low.sobsc:ured, Nosepiece is not corroctly ílngaged
Remody

Make sore that the revolving nosopiece

Page

v1 fluhJ u( viuw i::; uut cllcKs propor1y 1010 p1ace.

8
eventv 1Uum1nated.
The coodenser is not co1rectly •neched. Ae-•nsert the coodenser all the wav 4
without tilt.
The fieldins d1aptvaom rs not prooerty 1 Center che fieJdiris di.apht&gm 12
centered.(CH40 only)

The fietcl ons d,.phrogmos stoweddown Opon lile fiekf •ns draphregm 12
100 far.

Dart/dust on the obtect1ve , eveP•ece.

b 01rt or dust is visiblein
thG fte&d of v1ew.
ooodenser.an<Vor l>gh1 ex1t gss.
Clean thoooughly -
Oirt/dus-1on1he light exit gtass.
Oirt/dust on the toplens of the c:nn·
denser.
OirVdust on tho spoc1men.
Clean thoroughly.
-
-
Oirt/dust on the eyepiece.

c. The 1mage shows

dilfract.on
The condenser is k>wefed 100 far,
The apertute iris diaphragm •s stopped
Adju$t the condenser he1ght posiuo
Open the apen.ure iris diaphragm.
-
down too far.
12

d Visibil1ty 1$ poor The objectove,.noteo<rectly engogedin Make sure that the r evo1v1ng nosepe<:e 8
• lmage IS not sharp. thelight path, cides 1ntoplace correctty.
•Con1ras1is pOOt.
•OetaJts are indisonct Front leos ol the ob,ecb"" os dirw. Oean the objec....,, -
lmme1sion oil ts not betng used w.th an Use wnm.ersion Oll. 13
oil 1mmers100 ob;ective

lbe 1mmerst0n 0tl oonta1ns bubblos. Remove bubbles. 13

Recommended 1mme1S10n ollnot u:sed. Use the provided 1mmersion 011 -
Specimen is dlny. Clean. -
Eyepiece.condansor is dnty. Clean. -
e. Part of the image 1s The objective is not stopped at thee-tick· Make sure that the revolving nosoplece 8
lurred.
b stop. clicks into place correctlv.

Thé specimen is not mounted c0trec1ty P'8ce the specimen conectly on 1op of
oo the stage. 1he stage and secure 1t with the spec,. 10."
men holder
1 The omage hasayellow The bl\le fitter isnot engo(IOd. E"!l<'ge the btue htter. 5
"'"
 ., . '
Prob em Cause Remedy 1 Page
2. CoarseJFine Adjustme-nt

• The coarse adjustment The tension adjustment ring is over-

knobis hard to rurn. tightened.
Loasen thé ting. 13

You are tryulQ to raise the stege withthe Unlock the pre-tocusing !ever.
ooarse ad ustment knob even thoug.h the 13
P'trfocvs1ng kJver islocked.
b.Thestage drifrs dowt'I by 'fl:le tensioo aclJUStmen.t ringis too loose. Tighten the ring.
nself, or f ocus is tos1 13
during observa tion.
c. Coarse ad1us1ment v111 The pre focusingieveris keepingthe stage Unloc·k the pre focusinglever. 13
not goal! the v.1ay up. down.
d.Coarse adjustment will The condensar holder is too k>w. Raise the condenser holder. -
oot 90 a\J tha way down.
e. The objective makes The sp&e:imen is rnounteef ups1de-cto..vn. Mount the spec men correcttv.
eontact w 11h the speci. 10,11
meo before foc us is
obtained.

3. Observation Tube
s. F efd ot view of one eve The interpupiUary distanceis 1nco1roo:t. Ad¡ust the 1n erpupi113tY dista1 ce. 11
does not match that of
lhe other. lncorrect diopter adjustmen1. Adjust the diopter. 11

Oriferent eyep1eces are usect on the left

and ogh1.
Chango one eyopiece to match the other
so tha1 botl\ sides are the same.
-
The opticat axes are oot paraUéf. Upon looking into the eyepieces. try
l oking 81lh• overall f etd before
o
concefltratingon the specimenrange.You
may a so find ilhélpfultolook upand into
-
the distaoce for amoment before k>oking
backinto the microscope.

4.Stagc
lmage blurs as you The specimenis not correc tly positioned Mount the specimen oorrectfv.
move the speomen. on the stage, -
w 5. Objeetive Change
o
5
CJ
Ftont rens of
• high Specimen is mounted ups de-down.
power objectíve comes
Mount the specimen co((ecUy. 10,11

into contact w1th speci· Covet glass is 100 1hick. Usea O.17 mm thck cover glass.
men whén it tS engaged 11
¡:: after a tow poy1er objec-
tive

6. Electr eal Systcm

_,
"' a The bu bdoes no1light. Bulbis not mounted. Mount the designate-d bulb. 3
,_ The bulb is borned ovt.. Repla.ce the bu b. 3

llB b. Th& bulbIS dark

The power cord plug is not connected.

The voltage selector switch is set

incorrectly.
Conn&et the powor oord.

Set the sv1tch to matc h thelocal AC

mains.
5.6

c The bvlb burns out almost Wrong rvpe of bulb used. Use the oorrect bulb type. 3
immediately.
The voltage se ector sw.ich is set Set the switch to match thelocal AC
inconectly. maíns. i
 CH30/CH40
OPTIONAL VNJ TS

Optlonal Un t Application Remarks

Brightlield/darkfield pilase Centration of phase contrast
contrast attachment insert possibfe.
CH2-PCO Centrationof fieldiris diaphragm
not possible.

Ory darkfíeki condensar Centration of darkfield field of

BH-OCD view possible by us ng the
centering knobs of che con-
denser.

Phase contrast anachment Centration of phase contrast

BH2-PC insen possible.
Centrationof fietditís d aphragm
not possib e.

Simple polariz nglght attachment

set CH3·POL
The CH3-POL is províded with
the CH40. A ttach
ing the com-
*The standard condensar does
not allow polarized light
fContainsl pensating lens bek>w the CH3- observation.
Condenser CH3-COP CDP allows centration of the In case of CH30, centration of
Analyzer CH3-AN field iris diaphragm. the fie!diris diaphragm becomes
Polarizer U-POT possible if the compensat ng
lens and f ie d iris diaphragm
attachment are added.

o
o

e
This<levice complies wilh 1he requ rements ot both dlrecllve 89/336/EEC concemlngelecuomagnetic z
¡;j
compatibility and directive 73123/EEC conoern ng low voltage.The CE markingindicates comp!ianoe
with tha above dlfectives.
• PROPER SELECTION OF THE POWER SUPPLY CORO
lf no power supply cordis prov ded,please select the proper power supply cord for the equipment by referring
to "Specifications" and "Certif ed Cord" below:
CAUTION: In cese yOu use a non-approved power supply cord for Olympus products,Olympus can nolonger
warrant the electricalsafoty of the oquipment.

Specifications

Voltage Aating 125V ACifor 100-120V AC areal or,250V AC ifor 220-240V AC eres)
Cu"ent Rating 6A minimum
Temperature Rating 60ºC minimum
Léngth 3.05 m maximum
Fittings Conf 1guration Ground1ng rype attachmen1 plug cap.Opposite term
inatesin molded--on
IEC configuration appliance coupting.

Table 1 Certified Cord

A poi.ver suppty cord should be certified by one of the agenc1es listed 1n Tab e 1, or comprised of cordage
marked withanagency marking per Table 1or markeó per Table 2 .The fitt ngs areto be marked w ithat east
one of agencias tistedin Tab e 1.In case you ª'ª unable to buy loc.allyinvovr countcy the power SIJPPly cord
which is approved by one of the agencies mentioned in Table 1,please use replacements approved by any
other equivalent and authorized agencies in your countrv.

Certificat on Cercificat on
Country Agency Mark . Country Agency Mar·k

Ausualia SAA w ltaly IMO @

Avstua Ove <8> Japan MITI
w
Bcfgium CEBEC @J Nethertands KEMA
lhlm R

Ce11<1da CSA et!· Norway NEMKO

®
Denmaík DEMKO @ Spain AEE (i=i ee)
Finland FEI
® Sweden SEMKO
®
Franca UTE SW1tzer1and SEV (D
Germany VOE <fu United
Kingdom
ASTA
BSI .
líeland NSAI
1
U.S.A. Ul @
 1
CH30/CH40

Table 2 HAR Flexible Cord

APPROVAL ORGANIZAT ONS ANO CORDAGE HARMONIZATION MARKING METHOOS

Prlnte-d or emboued Harmo-- A

lternative Marking Utilizing
nizat1on Marking ( May be Black-Red-Vellow Thread
Approvat Organitation (Length of colorsection
inmm)
loctued on jacketor insulation
oflnternal w ir ng} Black Re<I Yellow
Comite Electrotechnique Beige
ICEBEC) CEVEC <HAR> 10 30 10

Verband Oevtscher E ektrotechnilcer

!VDEI e.V.Prüfstelle <VDE> <HAR::. 30 10 10

Union Technique de d'Electricite'

(UTE) USE <HAA> 30 30 10

Instituto Italiano del Marchio di

Ouali1a·(IMO) IEMMEOU <HAR> 10 30 50

British Approva s Service for Electr c

Cab esIBASEC) BASEC <HAR> 10 10 30

N.V ,l(EMA KEMA-l(EUR <HAR> 10 30 30

SEMKO AB Svenska Elek1riska

SEMKO <HAR> 10 10 50
Matenelk.ontrollanstalter

óste1reichischer Verband für

Elek1101echniklOVKJ
<ÓVE> <HAA> 30 10 so
Danmarks Elektt ske Materielk:ontrol
IDEMKO} <DEMKO> <HAR> 30 10 30

National Standards Authority of

lre and (NSA
l) <NSAI> <HAR> 30 30 50

Norges Elektrisk.e Materiellkontroll

NEMKO <HAA> 10 10 70
1NEMK0)

Asociacion Eleetrotecnica Y
<UNDE> <HAA> 30 10 70
E ewonica Espanola
IAEEI

Hellen c Organ zation for

Standardization (ELOT) ELOT <HAA> 30 30 70

Instituto Portugues da Qualidade

UPO) 1 np 1 <HAR> 10 10 90

Schweizerischer Elektro Techn scher

Verem (SEVJ SEV <HAR> 10 30 90

Efektriska lnspektoratet sen <HAR> 10 30 90

Undetwnters Laboiato1ies lnc. (UL> SV,SVT.SJ or SJT,3 X 18AWG

Canadian Standards Association (CSA) SV,SVT,SJ or SJT,3 X 18AWG
 OLVMPUSe

OlYMPUS OPTICALCQ,LTD.
2-43 2,Hatagaya,Shibuya
·ku,Tokyo,Japan
OLYMPUS OPTICALCQ (ElJROPA) GMBH.
Postfach 1O 49 08,20034,Hamburg,Germany
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OIYMPUS SINGAPORE PTE LTD.
4918 River Valley Road,#12-01/04 Valley Point Office Tower,S
ingapore 248373
OLYMPUS OPTICAL ca(UKJ l.TD.
2 8 Honduras Street,London EC1Y OTX, United Kingdom.
OIYMPUS AUSTRALIA PTY. LTD.
104 Femtree Gully Road,Oaklelgh,Victoria,3166,Australia

Thls pubtlcation Is pnnled on reo¡ded paper.

Printed fn Japen 2000 02 M 200-©