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Mantenimiento para HPLC
Mantenimiento para HPLC
M i t
Maintenance
Edward Kim
Application Engineer
Agilent Technologies, Inc
May 20, 2009
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Agilent
g e t Technologies
ec o og es
1100/1200 Series HPLC
Maintenance
Pump
Modules
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Agilent HPLC System Flexibility
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The Agilent 1200 LC Systems
One Comprehensive Series of Solutions for your Applications
High-resolution YES NO NO
column length 100 – 150mm Pressure limit too low Pressure limit too low
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Maximum Utilization of Entire Pressure Range
with Agilent 1100/1200 HPLC Systems
• Agilent 1100 and 1200 Instruments are Designed to Operate at Maximum
Pressure for Extended Periods
• Max Pressure Can be Maintained Across Full Flow Rate Range
– Flows of < 5mL/min Binary and < 10mL/min Quat are Easily Maintained
• Wide Flow Rate and Pressure Range Allows More Utilization
– One Instrument Configuration (1200SL) Can Run Current, Legacy and
Higher Performance Methods
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Quaternary Pump System
(G1354A)
Solvent Cabinet
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Quaternary Pump
Principle of Operation
AIV
MCGV
Purge Valve
MCGV Proportioning Valve
AIV Active Inlet Valve
OBV Outlet Ball Valve
OBV Damper
Vacuum Chamber
Authoring Division Name File Name
Security Notice (if required)
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Binary Pump
with Solvent Selection Valve
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Binaryy Pumpp
Principle of Operation
Damper
Purge
valve Mixer
A B
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1100 and 1200 Pumps
Exploded
p View
Holding Screw
Purge
Outlet Valve Valve
P
Pump H
Housing
i
Plunger Housing Seals
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Pump Check Valves
New style
G1312-60010
Cartridge
5062-8562 7
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Pump Head with Seal Wash
Exploded View
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Seal Wash
(Common to all 1100 pumps)
Washer
z Reduces seal wear when using mobile
phases
h with
ith salt
lt concentrations
t ti > 0.1
01M
Seal
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Agilent
g Technologies
g
1100 Series HPLC Maintenance
Autosampler
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Autosampler Plumbing
From pump
To column compartment
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Autosampler
P i i l off Operation
Principle O i
Standard loop volume300µl
Total delay volume 300µl + Vinj
Minimal (bypass) delay volume 6.2ul
Sampling
unitt
u
Rheodyne 7750
From pump
To column
z 4-port rotor seal
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Standard Injection Sequence
Metering
device
Sampling
unit
From pump
Prepare position
To column
6-port valve
To waste
Load position
0.35 0.35
0.3 0.3
0.25 0.25
Primidone Phenacetine
0.2 0.2
0.15 0.15
0.1 0.1
0 05
0.05 0 05
0.05
0 0
0.1 0.5 1 5 0.1 0.5 1 5
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Autosampler
p Special
p Functions
z NEEDLE WASH
to reduce carry-over to absolute minimum
z MULTI- DRAW
for injection volumes greater than 100ul
z BYPASS MODE
for minimum delay volume
z INJECTOR PROGRAM
f iinjection
for j ti sequence flexibility
fl ibilit
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Autosampler
Rheodyne 7750 Injection Valve
z Motor-driven (36V motor)
z Switch <150 ms.
z End positions detected by microswitches.
z Ceramic stator.
z Vespel and Tefzel rotor seals available.
z No set screws required.
z Complete assembly as repair part
part.
Ceramic stator
z Maintenance Positions:
– Change needle
– Change needle seat
– Change metering seal
– Change gripper arm
z Step commands
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Agilent
g Technologies
g
1100 Series HPLC Maintenance
Thermostatted
Column
Compartment
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Column Compartment
F t
Features
z Hold 3 30cm columns
z O
Operates
t from
f 10deg.
10d Cb below
l ambient
bi t ttemperature
t up tto 80
degrees C
z Peltier heating and cooling
z Two separate
p heating/cooling
g g zones
z Column ID module for column traceability
z Optional column switching valve
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Column ID Modules
Product number 79916OD-
Serial number 552 951001
Batch number 1675
Geometry [mm] 100x2.1
Stationary phase ODS
Particle size Hypersil
10 µm
Number of injections 999
Maximum pressure allowed [bar] 400
Maximum temperature recommended [°C] 70
Maximum pH recommended 12
ID ttag mustt b
be within
ithi 2mm
2 off the
th antenna,
t with
ith the
th HP logo
l up.
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Agilent
g Technologies
g
1100 Series HPLC Maintenance
Variable
Wavelength
g
Detector
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V i bl Wavelength
Variable W l th Detector
D t t
Principle
p
Deuterium lamp
Source lens
Filter (holmium/cutoff)
Entrance slit
Don't disturb it's
holder!
Beam splitter
Mirror #2
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Flow Cells
High Pressure
Standard and Micro
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Agilent
g Technologies
g
1100 Series HPLC Maintenance
Diode Array
Detector
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Diode Array
y Detector
Features
E
Extended
d d wavelength
l h range up to 950nm
9 0
Micro-mechanical slit
Improved noise specification
Ch i off flow
Choice fl cells
ll
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Diode Array
y Detector
Principle of Operation
Support lens
Grating
Holmium oxide
filter
Spectro
lens
Deuterium lamp
Flow cell
Array
Tungsten lamp
Source lens
(Achromat) Micro mechanical Slit
Micro-mechanical
Coupling lens
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Flow Cells
Standard and Semi-micro Micro
Maximum pressure 400 Bar
z Maximum pressure 120 Bar
z M t i l SST,
Materials: SST quartz,Teflon
t T fl M t i l SST,
Materials: SST quartz,
t Kapton
K t
z Apertures of front and rear
6mm, 1.7ul
gaskets have different diameters
z Standard cell uses cell body for
additional
dditi l heat
h t exchange
h
z Semi-micro cell: 6mm, 5uL
z Standard: 10mm, 13uL
Mi
Micro C
Cellll
Different apertures
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Chromatographic Results with Second Source
Lamp at 280 nm Wavelength - Small Peaks
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Second Long-Life
g Lampp - 2000 Hours
PN: 2140-0813 (March 2003)
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Summary
Over the past years we had been taken many efforts, invested money,
time and resources to make sure that we always have the best lamps for
our customers and made improvements for all our lamps.
Longer life time of our lamps reduces the cost of ownership of our
instruments substantially.y
We also make sure that we will continue our efforts to make the best
lamps available to our customers in the future.
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