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Exp 5 Carbfh
Exp 5 Carbfh
Exp 5 Carbfh
Experiment No. 5
CARBOHYDRATES
I. Objectives
1. To observe reaction of different carbohydrates using different test such as (a) Molish
Test (b) Benedict’s Test (c) Fehling’s Test (d) Barfoed’s Test (e) Picric Test (f) Iodine
Test (g) Seliwanoff’sTest (h) Phenylhydrazine
2. To examine the crystals formed under a low-power microscope
3. To draw and describe the crystals that is observe
4. To identify the unknown sugar
grfggger
II. Procedures
I. Mollsch Test:
- Label six test tubes with number 1-6. Place 1ml of different sugar
solutions into five test tubes. Add distilled water to the sixth test tube to
serve as control.
- To each test tubes, add 3 drops of Mollsch reagent. Shake each test
tube to ensure mixing.
- With the test tube held at a 45 degree angle, carefully and slowly run
10 drops of concentrated H2SO4 down the wall of the test tube so
that two layers form. Note the color at the point where the two layers
meet.