SET A Reg.No.

VIVEKANANDHA COLLEGE OF ENGINEERING FOR WOMEN

[AUTONOMOUS INSTITUTION AFFILIATED TO ANNA UNIVERSITY, CHENNAI]
Elayampalayam – 637 205, Tiruchengode, Namakkal Dt., Tamil Nadu.

INTERNAL TEST– III (B.E. / B.Tech)

(2015-Regulation)
Department of Biotechnology
U15BT406- BIOPROCESS ENGINEERING
Time : 1.30 Hours Maximum : 40 Marks
Answer ALL the questions
PART – A
(5 x 2 = 10 Marks)
Q.No. CO
Questions KL
Attainment
Write equation of extraction distribution coefficients for weak
1. KL1 CO5
acids and bases.
2. Cephalosporin is separated from fermentation broth by
adsorption on an ion-exchange resin in a moving-bed column.
The bed is 4 cm in diameter and contains 0.8 cm3 resin/cm3
bed. The density of the resin is 1.3 g/cm3, and the feed solution
contains 5 g/l of the antibiotic. If the superficial velocity of
liquid is 1.5 m/h and the overall mass transfer coefficient is 15
KL2 CO5
h-1, determine the height of the column for an effluent antibiotic
concentration of CL = 0.2 g/l. Assume an equilibrium
relationship of
CS = 25(CL*)1/2
where CS is g solute/l resin and CL is g solute/l solution. The
ratio of volumetric flow rate of broth to resin is 10.
3. Give any four Non-mechanical Methods of cell disruption. KL1 CO5
4. Identify the type of centrifuge by direction and symbol in the
fig. and indicate what the symbol denotes?

KL2 CO5

A B
5. Draw a Liquid-liquid extraction equipment (Podbielniak) KL1 CO5

1
 PART – B
(2 x 15 = 30 Marks)
Q.No CO
Questions KL
. Attainment
6. a.

KL4 CO5

(OR)
b. Cell-free fermentation liquor contains 9x10-5mol.l-1
immunoglobulin G. It is proposed to recover at least 80% of
this antibody by adsorption on synthetic, non-polar resin.
Experimental equilibrium data are correlated
as follows:
CO5
C*AS = 4.5 x 10-5 C*A 0.35, where C*AS is mol solute adsorbed KL4
per cm3 adsorbent and C*A is liquid-phase solute
concentration in mol 1-1. What minimum quantity of resin is
required to treat 5m3 fermentation liquor in a single-stage
mixed tank?
7. a. i) Explain the two phase aqueous extraction used for the
purification of biomolecules.
CO5
ii) Give detailed notes on theory of filtration and briefly describe
KL4
about batch filters.
(OR)
b. i) Explain working principle of ion exchange chromatography
along with its applications.
KL4 CO5
ii) Explain working principle of affinity exchange chromatography
along with its applications.

STAFF INCHARGE HOD/BT