How to make a "loading pen" How to prepare PTLC

(1) (2) (3)

(1) (2) draw guideline
by pencil
nipple
cotton make half
with cutter

1~1.5 cm
for authentic
Pasteur
sample
pippette
cut with 20cm x 20cm TLC
ample
cutter

How to load your sample

(1) (2) (3) (4)

push
slowly go development !
spot authentic sample
next to the sample band
suck up

repeat

sample
solution draw along
the guideline
How to detect PTLC band

1st: Check UV (254 nm) 2nd: Check TLC stain (as you like)

(1) (2) (3)

burn the TLC
cotton on hot plate
mark the band
soaked with
by pencil
TLC stain

background = green cut minimum edge of

UV active = black (no FL) apply the stain detect !
developed PTLC

Purpose of protocol

# PTLC (preparative TLC) is used for purification of sample in tough-separable mixture.

# Primary purpose is getting analytically pure sample.

# Usually you cannot determine precise yield because of inefficient extraction from scraped silica gel.

TIPS
# Generally, it takes very long time to develop full-size PTLC. This may cause undesired decomposition.
Half-size PTLC is highly recommended.

# If the sample is difficult to be separated, develop twice or more than using big TLC. This is faster way !

# Recommended loading amount : 0.5mm thick = < 10 mg; 1.0 mm thick = < 50 mg
(Max. loading amount : 0.5mm thick = 25 mg; 1.0mm thick = 100 mg)