Comparison Chart of Gram +ve & - Ve

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Comparison chart

Gram-negative Bacteria versus Gram-positive Bacteria comparison chart

Gram-negative Gram-positive
Bacteria Bacteria
Gram reaction Can be decolorized to accept Retain crystal violet dye and
counterstain (safranin) and stain stain blue or purple
pink or red

Cell Wall They have a wavy and double- Their cell wall is smooth and
layered cell-wall single-layered

Cell Wall thickness The thickness of the cell wall is 8 to The thickness of the cell wall
10 nanometres is 20 to 80 nanometres

Peptidoglycan Thin (single-layered) Thick (multilayered)


layer

Lipid and 20 to 30% Very low


lipoprotein content

Lipopolysaccharid High Virtually none


e (LPS) content

Teichoic acids Absent Present in many

Periplasmic space present Absent

Outer membrane Present Absent

Mesosome Mesosome is less prominent. Mesosome is more


prominent.

Porins Occurs in Outer Membrane Absent

Flagellar structure 4 rings in basal body 2 rings in basal body

Morphology Non-spore forming rods Cocci or spore-forming rods


Gram-negative Bacteria versus Gram-positive Bacteria comparison chart

Gram-negative Gram-positive
Bacteria Bacteria
Toxins produced Primarily Endotoxins Primarily Exotoxins

Resistance to Low High


physical disruption

Inhibition by basic Low High


dyes

Susceptibility to Low High


anionic detergents

Resistance to Low High


sodium azide

Resistance to Low High


drying

Cell Wall Low (requires pretreatment to High


Disruption by destabilize outer membrane)
Lysozyme

Antibiotic More resistant More susceptible


Resistance

Examples Escherichia, Salmonella, etc. Staphylococcus,


Streptococcus, etc.

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