BioLit 160 – Homework 5 (5 points) Name: Reagan Grady

Fall 2018

Read the Abstract, Introduction, Methods (2.1, 2.2, 2.3, 2.5, 2.6, and 2.10 only of the Methods), and
Results (3.1 – 3.3 only of the results) of Ali, I. et al. Biochemical responses and ultrastructural changes in
ethylene insensitive mutants of Arabidopsis thialiana subjected to bisphenol A exposure. 2017. 144:62-
71. Answer the following questions and turn in a typed, hard copy to your BioLit instructor at the
beginning of class.

1. How are plants affected by high doses of BPA? (0.5 pt)

They are hurt from the BPA in their growth through oxidative stress.

2. Ethylene is a plant hormone that is produced in excess in plants under stress. How are the
mutants (ein2-1 and etr1-3) different from wild-type? (1 pt)
The mutants are different than the wild-type in that they had significantly less damage from
oxidative stress because they have better antioxidant defense systems.

3. What is the research question that the authors addressed in the study? (0.5 pt)
What is the role, if any, BPA plays when inducing oxidative stress in Arabidopsis thaliana plants?

4. Identify one of the possible claims for each of the following figures. Also, describe how the
evidence in the figure supports the claim you identified. (3 pts)

Figure Claim Describe how the evidence supports your claim

The fresh weight of WT The letters indicated significant differences in the
and ein2-1 mutant numbers.
Figure significantly decreased
1B when increased from 20
µM BPA exposure to 30
µM BPA exposure.
The chlorophyll b The letters indicated significant differences in the
concentration of WT numbers.
Figure mutant significantly
2A or decreased when
2B increased from 10 µM
BPA exposure to 20 µM
BPA exposure.
There is a significant The letters indicated significant differences in the
difference in the MDA numbers.
Content of WT when BPA
concentration is
increased from 20 µM
Figure 3 BPA exposure to 30 µM
BPA exposure.