Skoog, 9th ed.

24.18 Evaluate the missing quantities in the accompanying table. Where needed, use 200 for the molar
mass of the analyte.

ꜫ b c

A %T L mol-1 cm-1 cm-1 ppm-1 cm M ppm

0.172 4.23x103 1.00

a.)
44.9 d 0.0258 1.35x10-4
b.)

3.73x103 0.100 1.71x10-3

e.)
83.6 1.00 8.07x10-6
f.)
Group 12 [Ong + Santos] Feb 20 (Wed)
0.798 1.50 33.6
g.)
11.1 1.35x104 7.07x10-5
h.)

24.20 Beryllium (II) forms a complex with acetylacetone (166.2 g/mol). Calculate the molar absorptivity
of the complex, given that 2.25 ppm solution has a transmittance of 37.5% when measured in a 1.00-cm
cell at 295 nm, the wavelength of maximum absorption.

Grp 13 [Yee + Reyes] Feb 20 (Wed)

(c) the transmittance of the solutions described in (a) and (b).

Sample Exercise: Spectroscopic analysis (Single point calibration)

Harris, 7th ed.

Correct solution:

1 x 10−6 L 1000 mL
C std =
10.0 μL ( 1 μL )( 1L ) −3
7.50 x 10 M
=1.5 x 10−6 M
50.0 mL

A
ꜫ=
bc

0.967−0.153
ꜫ= −6
=1.085 x 105 cm−1 M −1
( 5.00 cm ) ( 1.5 x 10 M )

The Absorbance of the blank should be subtracted to the Absorbance of the sample.

A
c=
ꜫb

0.622−0.153
c= 5 −1 −1
=8.65 x 10−7 M
( 5.00 cm ) ( 1.085 x 10 cm M )

mol colored complex 1 mol analyte 46.005 g

8.65 x 10−7 x x
L 1 mol colored complex 1 x 10 6 μg
1 mol NO 2−¿ x x 0.050 L ¿
1g
= 1.99 µg nitrite ion
Grp 15 [Audan + Calumpong] Feb 22 (Fri)

Grp 16 [Natividad + Manzo + Macapagal] Feb 22 (Fri)