IAL BIOLOGY UNIT 3

FREQUENTLY ASKED QUESTIONS

WORKING OF A COLORIMETER
Use a colorimeter to measure the colour intensity in arbitrary units.
Shake before measurement
Use of appropriate colour filter
Zeroing against blank
Shine light through the sample and measure the absorbance of light
RISK AND METHODS TO PREVENT IT
Hot water/scalding risk – wear a lab coat/ (heat resistant) gloves
(Bunsen) risk of burning – keep away from flame
Alkaline solution risk to skin or eyes – wear goggles or gloves/lab coat/wash
affected area immediately
Skin contact allergy – wear gloves/lab coat/wash affected area immediately/use
coated enzyme.
Inhalation allergy – wear mask/use coated enzyme
Spillages – clean up immediately
Ethanol is flammable – avoid contact with flame
Getting enzyme into blood through cut – wear plaster/gloves
CHECK IF AN EXPERIMENT IS RELIABLE
Check number of experiments performed for each independent variable, if large
amount, experiment is reliable.
Average found, reliable.
Sample size is large, reliable
No overlap
CHECK IF EXPERIMENT IS VALID
For section 1
Use of control hence valid
Comparing results, similar hence valid
No overlap hence valid
Standard deviation high, validity reduces.
For section 2
Check for any bias of sponsor
Check for credentials of contributor
Cross checking with another source
Evidence of peer reviewing
CONTROL OF VARIABLES
Temperature
Using water bath
Performing experiment inside a sealed room where temperature is constant
pH
Using a pH buffer
Light intensity
Idea of same light source e.g. light bulb
Idea of how same intensity/same distance
CONTROL OF VARIABLES
Humidity
Closed container/reduces evaporation
Carbon dioxide level
Cylinder/enclosed chamber
Wind speed
Cylinder/enclosed chamber
HOW TO CALCULATE DRY MASS
Remove water by placing in oven for sensible time, until constant mass
HOW TO MEASURE AREA OF NON-SYMMETRICAL
INHIBITION
Use graph paper, tracing diameter
Count squares
DIFFERENT IMPLICATION AND THEIR DEFINITION
Ethical implication
About the law e.g. ethics of keeping animals in zoo
Social implication
About the effects towards society e.g. educating the society
Environmental implication
Things that affect the environment both positive and negative
Economical implication
Money spent on projects
METHODS OF IMPROVING STUDY
Bigger sample size
Use of matched populations
Using people who have not come from failed drug treatment
Longer term study
More objective method of measurement of improvement needed
Double blind test stated
REFERENCE ORDER
1. Author
2. Year in brackets
3. Title
4. Journal
5. Volume
6. Issue in brackets
7. Page number