Urgent Cross-match

Cases:-

 In the first request of blood transfusion.

 Sever hemorrhage.

Procedures:-

No. of tubes 1 R.T 2 Alb 3 C

Patient' serum 2 drops 2 drops 2 drops
Patient's cells --------- --------- 2 drops
Donor's cells washed 2 drops 2 drops ---------
3 times
Albumin 30% --------- 1 drop ---------
* R.T = Room Temperature , Alb= Albumin , C= Control

- Incubate Alb tube in water bath at 37 C for 15 min.

- The reminder tubes: R.T. and C. in room temperature at 25 C for 15 min.
- Centrifuge all tubes.
- Examine under microscope.
The result:-

 If there is agglutination :- Uncombatible

 If there is no agglutination:- Combatible