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Hem 323 Week 7 Lec I Laboratory Evaluation of Hemostasis
Hem 323 Week 7 Lec I Laboratory Evaluation of Hemostasis
Hem 323 Week 7 Lec I Laboratory Evaluation of Hemostasis
1. EDTA
Don’t preserve labile clotting factors
Inhibits thrombin-fibrinogen reaction
Only for platelet counts; NOT for coagulation studies
2. Heparin
Binds with anti-thrombin III
Inhibits all reactions in coagulation
For platelet retention test/glass bead retention test only
3. Oxalates
Shortened clotting time because it forms insoluble complexes/precipitates
ETS is preferred than syringe method: it is because blood directly mixes with the anticoagulant reducing
chances of clotted samples
ORDER OF DRAW FOR COAGULATION TESTING:
1. Red top tube
2. Light Blue
3. EDTA
4. Plastic syringe
DRUGS TO BE AVOIDED
1. Aspirin
Inhibits cyclooxygenase
platelet aggregation test:
Bleeding time:
2. Heparin
Affects coagulation studies
Inhibits most clotting factors
3. Warfarin/Coumadin
Antagonist of prothrombin group/Vit K. dependent clotting factors
Prevents gamma-carboxylation of vitamin K on clotting factors
4. Penicillin
Alters blood vessel walls
Induces hemolysis