Thymox-EPO    

Thymox-EPO is a dry powdered extract obtained from Thymus vulgaris L aerial parts by

hydroalcoholic (ethanol 70% - water 30%) extraction (general method according to Eur. Ph. 8.0). It
is standardized to contain 0.3 % total phenols, calculated as thymol (spectrophotometric method,
DAB 10).

The botanical species is certified by DNA barcoding analysis. No harmful solvent is used in the
manufacturing process, which is entirely made in Italy, with full traceability from the field to the
final packaging and a production chain that is checked at every stage. The quantification of bioactive
compounds is accurately performed by sophisticated analytical methods and in compliance with EU
legislation on food and food supplements.

Its efficacy is based on preclinical evidence, providing its double effect as an anti-inflammatory and
expectorant agent, which makes Thymox-EPO suitable for respiratory tract inflammations, asthma,
dry and productive cough.

 Activity  

Thymox-EPO  has been tested on 3D in vitro cellular model, which faithfully reproduces the
physiological condition.  It was demonstrated that Thymox-EPO has a good expectorant and anti-
inflammatory activity.

Thymox is able to reduce inflammation induced by LPS (Lypopolysaccharide) treatment on human

respiratory epithelial cells (11), by decreasing pro-inflammatory mediator proteins as NF-kB p65 and
NF-kB p52 and pro-inflammatory cytokines IL-1β and IL-8, at both mRNA and protein levels,
evidencing so a strong anti-inflammatory property.

The respiratory tract has epithelial cells that are characterized by cilia on the surface. Expectoration
consists in the movement of mucus through the respiratory tract thanks to the efficient beating of the
cilia a Ca2+-mediated mechanism.

Thymox was shown to increase extracellular cAMP levels; it increases Ca 2+influx while decreasing
extracellular Ca2+level; therefore, enhancing ciliary beating frequency. By increasing the frequency
of beating, Thymox-     EPO helps mucus clearance (expectoration).

The recommended daily dosage is 10 g drug with 0.03% phenol, calculated as thymol, corresponding
to 1 g of Thymox-EPO (Thyme dry extract 0.3%).