APPENDICES

oo
O
O
CD
Absorbance 600 nm
O
O
CM

0.0 1 1 1 1
w----------------------- ------------------------ ------------------------ ------------------------ ------------------------

0 2 4 6 8 10

Ethanol (%) (v/v)

Appendix 1: Standard curve of ethanol by the method of Caputi et al., (1968).

 0.4

0.3
Absorbance at 680 nm

0.2

0.1

0.0
0 50 100 150 200 250

BSA (jag)

Appendix 2: Standard curve of protein by the method of Lowry et al., (1951).

 Absorbance at 765 nm

0.2 - /

0.0 V—------------------- 1------------------------ 1------------------------ 1------------------------ 1------------------------

0 20 40 60 80 100

Gallic acid (ug)

Appendix 3: Standard curve of gallic acid by the method of Waterhouse, (2002)

 0.8
O
CD
Absorbance 740nm
O
O
CM

0.0
0.00 0.02 0.04 0.06 0.08 0.10 0.12

FeS04.7hL0 (pmoles)

Appendix 4: Standard curve of FeS04.7H20 (pinoles) by the method of Benzie and Stra

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■v / ' '

f? ■' :

■ k .